Rroxscaffold_1G00044400

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
N/A
Physical Location & Seq
Forward (+)
63375047 .. 63377383
2337 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00044400.1

Sequence Viewer

Length: 726 bp
ATGACTGTGACAGGTCATGCTAATTTTGATATTAGAGGAGAAGGAAGTGGGTGCATACTCTGGCTTGGAGAACTGATTGATACTCGAGAATTCTCTGATGCTGGGCAGGACATATATATAAGGATGGCTGCCTCTGAGCTAGTGACATACAGAAGCTTGAAGGGAAAGACAAAAGTTGAGGTCATAGTTCTATCTGTATTAGCTGTGGGCATAATTCTTCTAGGCCAATGCTTGATACTACATTTATACAAGAGGAAGAAGAAGAAGAAGAAGCAGATTAAAAGAAAGTTAGGAAATATGTTTCATACTCCAGAGCAAGACAGCAATAACGAATGCCAAGATGAACATCTAGAGCTACCATTATTTGGTTTTAGTACTATAGCAGATGCCACAAATAATTGTTCTGTTGCTAACAAGCTTAAAAAGGGTGGTTTTGGAGGGAAAACTGATGGAGGGGCAAGAAATAGGGGTGAAAAGGCTTTCAAGAACGGTTACATGTCTCTGGAATATGCAATTGATGGTGTCTTCTCGGTGAATTCTGATGTTTTTAGCTTTGGTGTTTTGGTACTTGAGATAGTGAGTGGAAAGAAGAACAGAGGATATTTTCACCCAGACTACAAGCTTAACCTTCTTGGACATGCCTGGAGGCTCTTCAAAGAAGGCAAGCCATTTGAAATGATGCATACATCTATAAAGCACAAAGACGCAAGCAGTATGTCCAAGTGA

Protein Analysis

241

Amino Acids

26.88

Weight (kDa)

9.36

Isoelectric Point (pI)

26.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 153 - 198 1.2e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 365
AcsI RAATTY 2 cut(s) 89, 535
AfaI GTAC 2 cut(s) 376, 567
AfiI CCNNNNNNNGG 1 cut(s) 365
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 4 cut(s) 160, 484, 655, 674
AjnI CCWGG 1 cut(s) 641
AluBI AGCT 7 cut(s) 139, 156, 203, 355, 418, 552, 622
AluI AGCT 7 cut(s) 139, 156, 203, 355, 418, 552, 622
Alw26I GTCTC 1 cut(s) 504
Ama87I CYCGRG 1 cut(s) 84
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 1 cut(s) 128
ApoI RAATTY 2 cut(s) 89, 535
AsuHPI GGTGA 3 cut(s) 482, 544, 599
AvaI CYCGRG 1 cut(s) 84
BbsI GAAGAC 1 cut(s) 517
BbvI GCAGC 1 cut(s) 115
BccI CCATC 3 cut(s) 118, 443, 512
BciT130I CCWGG 1 cut(s) 643
BcoDI GTCTC 1 cut(s) 504
BfaI CTAG 3 cut(s) 140, 221, 350
BfmI CTRYAG 1 cut(s) 378
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
BmcAI AGTACT 1 cut(s) 376
Bme1390I CCNGG 1 cut(s) 643
BmeT110I CYCGRG 1 cut(s) 84
BmrFI CCNGG 1 cut(s) 643
BmsI GCATC 3 cut(s) 88, 376, 669
BpiI GAAGAC 1 cut(s) 517
BpmI CTGGAG 2 cut(s) 294, 664
BpuEI CTTGAG 1 cut(s) 590
BsaBI GATNNNNATC 1 cut(s) 345
Bsc4I CCNNNNNNNGG 1 cut(s) 365
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 1 cut(s) 643
BseGI GGATG 1 cut(s) 129
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 1 cut(s) 365
BseMII CTCAG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 51
BseXI GCAGC 1 cut(s) 115
BseYI CCCAGC 1 cut(s) 101
BshFI GGCC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 365
BsmAI GTCTC 1 cut(s) 504
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 1 cut(s) 225
BsoBI CYCGRG 1 cut(s) 84
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 1 cut(s) 127
BspQI GCTCTTC 1 cut(s) 656
Bst2UI CCWGG 1 cut(s) 643
Bst4CI ACNGT 2 cut(s) 7, 491
Bst6I CTCTTC 1 cut(s) 656
BstC8I GCNNGC 2 cut(s) 665, 709
BstDEI CTNAG 1 cut(s) 135
BstF5I GGATG 1 cut(s) 129
BstMAI GTCTC 1 cut(s) 504
BstNI CCWGG 1 cut(s) 643
BstNSI RCATGY 2 cut(s) 499, 641
BstSCI CCNGG 1 cut(s) 641
BstSFI CTRYAG 1 cut(s) 378
BstV1I GCAGC 1 cut(s) 115
BstV2I GAAGAC 1 cut(s) 517
BsuRI GGCC 1 cut(s) 225
BtsCI GGATG 1 cut(s) 129
Cac8I GCNNGC 2 cut(s) 665, 709
CseI GACGC 1 cut(s) 713
Csp6I GTAC 2 cut(s) 375, 566
CviAII CATG 3 cut(s) 17, 496, 638
CviQI GTAC 2 cut(s) 375, 566
DdeI CTNAG 1 cut(s) 135
Eam1104I CTCTTC 1 cut(s) 656
EarI CTCTTC 1 cut(s) 656
Eco88I CYCGRG 1 cut(s) 84
EcoRI GAATTC 2 cut(s) 89, 535
EcoRII CCWGG 1 cut(s) 641
EcoT22I ATGCAT 1 cut(s) 684
FaeI CATG 3 cut(s) 20, 499, 641
FatI CATG 3 cut(s) 16, 495, 637
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 1 cut(s) 136
Fsp4HI GCNGC 1 cut(s) 129
FspBI CTAG 3 cut(s) 140, 221, 350
GluI GCNGC 1 cut(s) 129
GsaI CCCAGC 1 cut(s) 105
GsuI CTGGAG 2 cut(s) 294, 664
HaeIII GGCC 1 cut(s) 225
HgaI GACGC 1 cut(s) 713
Hin1II CATG 3 cut(s) 20, 499, 641
HindIII AAGCTT 3 cut(s) 154, 416, 620
HphI GGTGA 3 cut(s) 482, 544, 599
Hpy188I TCNGA 3 cut(s) 97, 136, 541
Hpy188III TCNNGA 5 cut(s) 86, 311, 350, 484, 503
HpyAV CCTTC 4 cut(s) 35, 154, 638, 653
HpyCH4III ACNGT 2 cut(s) 7, 491
HpyCH4V TGCA 3 cut(s) 54, 512, 682
HpyF3I CTNAG 1 cut(s) 135
Hsp92II CATG 3 cut(s) 20, 499, 641
LguI GCTCTTC 1 cut(s) 656
LpnPI CCDG 8 cut(s) 46, 87, 92, 324, 488, 624, 628, 655
Lsp1109I GCAGC 1 cut(s) 115
LweI GCATC 3 cut(s) 88, 376, 669
MaeI CTAG 3 cut(s) 140, 221, 350
MaeIII GTNAC 3 cut(s) 7, 142, 491
MboII GAAGA 9 cut(s) 209, 268, 271, 274, 277, 280, 517, 601, 643
MfeI CAATTG 1 cut(s) 513
MluCI AATT 6 cut(s) 22, 89, 213, 397, 513, 535
MnlI CCTC 8 cut(s) 29, 142, 172, 246, 431, 446, 590, 639
Mph1103I ATGCAT 1 cut(s) 684
MseI TTAA 3 cut(s) 279, 420, 624
MspR9I CCNGG 1 cut(s) 643
MunI CAATTG 1 cut(s) 513
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 1 cut(s) 643
NlaIII CATG 3 cut(s) 20, 499, 641
NmuCI GTSAC 2 cut(s) 7, 142
NsiI ATGCAT 1 cut(s) 684
NspI RCATGY 2 cut(s) 499, 641
PaeR7I CTCGAG 1 cut(s) 84
PciI ACATGT 1 cut(s) 495
PciSI GCTCTTC 1 cut(s) 656
PctI GAATGC 1 cut(s) 338
PflMI CCANNNNNTGG 1 cut(s) 365
PkrI GCNGC 1 cut(s) 130
PscI ACATGT 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 641
PspFI CCCAGC 1 cut(s) 101
PspGI CCWGG 1 cut(s) 641
RsaI GTAC 2 cut(s) 376, 567
RsaNI GTAC 2 cut(s) 375, 566
SapI GCTCTTC 1 cut(s) 656
SaqAI TTAA 3 cut(s) 279, 420, 624
SatI GCNGC 1 cut(s) 129
ScaI AGTACT 1 cut(s) 376
ScrFI CCNGG 1 cut(s) 643
SfaNI GCATC 3 cut(s) 88, 376, 669
SfcI CTRYAG 1 cut(s) 378
Sfr274I CTCGAG 1 cut(s) 84
SlaI CTCGAG 1 cut(s) 84
SmlI CTYRAG 2 cut(s) 84, 569
SmoI CTYRAG 2 cut(s) 84, 569
Sse9I AATT 6 cut(s) 22, 89, 213, 397, 513, 535
SspMI CTAG 3 cut(s) 140, 221, 350
StyD4I CCNGG 1 cut(s) 641
TaaI ACNGT 2 cut(s) 7, 491
TaqI TCGA 1 cut(s) 85
TasI AATT 6 cut(s) 22, 89, 213, 397, 513, 535
TatI WGTACW 1 cut(s) 374
Tru1I TTAA 3 cut(s) 279, 420, 624
Tru9I TTAA 3 cut(s) 279, 420, 624
TseFI GTSAC 2 cut(s) 7, 142
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 2 cut(s) 7, 142
TspDTI ATGAA 2 cut(s) 293, 357
Van91I CCANNNNNTGG 1 cut(s) 365
XapI RAATTY 2 cut(s) 89, 535
XbaI TCTAGA 1 cut(s) 349
XceI RCATGY 2 cut(s) 499, 641
XhoI CTCGAG 1 cut(s) 84
XspI CTAG 3 cut(s) 140, 221, 350
ZrmI AGTACT 1 cut(s) 376
Zsp2I ATGCAT 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.