Rroxscaffold_1G00053960

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
N/A
Physical Location & Seq
Forward (+)
75216016 .. 75224621
8606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00053960.1

Sequence Viewer

Length: 906 bp
ATGATCAACTTATTGTGCTTGCTCATCGGTTTCTCAAGCTTCGGACTTGTCATGGTGCAGCTACACTGTTCATTTGGAGACACGCTTAATCCGGGGGAGGCCCTCAATTCCTCGAGCTTTATGGTCTCCACTTCGGGGGAGTTTACGTTGGGTTTCAACTCGGTTACCTCATTCGAGGTGCAAGAACTATACGGTTTGGGTTGCCAACCTGCACACTCCGATTTCGGACCCTTCGTCGGCGCTTCTCACCTTAGACAAGAAACAAAACTCTCCTGCAAATTACCCCGAAAGGCAAGAATCCCATCCGTGGTTTCATATGAGAATGGAGACTACTTCGGTCACACGGTCGTTGGTAATCCAAATAGCAGATCCTCTTGGGTGCTAGACACGGGCGGAAAGCTTTATGATGTTTACTATAACATTTCTATTGCGTCACGGGTGAGTGATGGCTATAACCCCGATGGTGGTGGGACGTGCGAGAGATGGAGTGGCGGGCCACTCTTTGTTGTCTGTGGCAACATCCCTATGGTGCATTTATTTAGCGAGAGAGAAAGGCGCTCCCTTAGACCAATCGGTATGAACATGTCTATCAATTCAACCTGGGAGGAGACAAATTCAAGTCGATATGGGATTTATGAATGTATGGCTACCTGTTGGATGGACTGTGACTGCCTTGGCTTCGAATTTCTCTTTGATAATCGGATTTGGATGTACGTCGATTTTGGAGTTAATGTTCTACTTGATGAAAGCATGAACCCCAAAATTTCCGATTTTGGAATGGCGAGGATCTTCACGAATGATGAACAAGAAGCAAATACTATGAAGATTGTTGGGACATGTGTCTTCCCGAAGAACATGCGGGATCACAAAGTGAACTGTGGGTCCCTTGACAAAATCTATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

301

Amino Acids

33.53

Weight (kDa)

5.16

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 217
AciI CCGC 3 cut(s) 393, 492, 859
AclWI GGATC 3 cut(s) 363, 794, 870
AcsI RAATTY 3 cut(s) 613, 683, 762
AdeI CACNNNGTG 1 cut(s) 871
AfaI GTAC 1 cut(s) 713
AfiI CCNNNNNNNGG 4 cut(s) 135, 236, 307, 464
AflIII ACRYGT 2 cut(s) 582, 836
AgsI TTSAA 3 cut(s) 157, 597, 618
AhdI GACNNNNNGTC 1 cut(s) 233
AjiI CACGTC 1 cut(s) 474
AjnI CCWGG 1 cut(s) 599
AloI GAACNNNNNNTCC 4 cut(s) 717, 749, 866, 898
AluBI AGCT 4 cut(s) 39, 61, 117, 400
AluI AGCT 4 cut(s) 39, 61, 117, 400
Alw26I GTCTC 4 cut(s) 72, 130, 321, 602
AlwI GGATC 3 cut(s) 363, 794, 870
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 2 cut(s) 99, 494
ApeKI GCWGC 1 cut(s) 58
ApoI RAATTY 3 cut(s) 613, 683, 762
AspLEI GCGC 2 cut(s) 242, 558
AspS9I GGNCC 4 cut(s) 100, 227, 494, 882
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 2 cut(s) 239, 451
AsuII TTCGAA 1 cut(s) 681
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 2 cut(s) 227, 882
BbsI GAAGAC 1 cut(s) 835
BbvI GCAGC 1 cut(s) 70
BccI CCATC 5 cut(s) 310, 440, 455, 477, 652
BcgI CGANNNNNNTGC 2 cut(s) 838, 872
BciT130I CCWGG 1 cut(s) 601
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 4 cut(s) 72, 130, 321, 602
BfaI CTAG 1 cut(s) 383
BfoI RGCGCY 2 cut(s) 243, 559
BfuAI ACCTGC 1 cut(s) 217
BisI GCNGC 1 cut(s) 59
BlsI GCNGC 1 cut(s) 60
Bme1390I CCNGG 2 cut(s) 93, 601
Bme18I GGWCC 2 cut(s) 227, 882
BmeRI GACNNNNNGTC 1 cut(s) 233
BmeT110I CYCGRG 1 cut(s) 112
BmgBI CACGTC 1 cut(s) 474
BmgT120I GGNCC 4 cut(s) 100, 227, 494, 882
BmiI GGNNCC 3 cut(s) 229, 883, 884
BmrFI CCNGG 2 cut(s) 93, 601
BoxI GACNNNNGTC 1 cut(s) 839
BpiI GAAGAC 1 cut(s) 835
Bpu14I TTCGAA 1 cut(s) 681
BpuEI CTTGAG 1 cut(s) 19
BpuMI CCSGG 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 4 cut(s) 92, 306, 600, 673
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 717, 747
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 236, 307, 464
BseBI CCWGG 1 cut(s) 601
BseDI CCNNGG 4 cut(s) 92, 306, 600, 673
BseGI GGATG 4 cut(s) 302, 519, 663, 714
BseLI CCNNNNNNNGG 4 cut(s) 135, 236, 307, 464
BseRI GAGGAG 1 cut(s) 620
BseXI GCAGC 1 cut(s) 70
BsgI GTGCAG 2 cut(s) 77, 195
Bsh1285I CGRYCG 1 cut(s) 348
BshFI GGCC 2 cut(s) 101, 496
BsiEI CGRYCG 1 cut(s) 348
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 3 cut(s) 484, 847, 868
BslI CCNNNNNNNGG 4 cut(s) 135, 236, 307, 464
BsmAI GTCTC 4 cut(s) 72, 130, 321, 602
BsmFI GGGAC 3 cut(s) 484, 847, 868
BsnI GGCC 2 cut(s) 101, 496
Bso31I GGTCTC 1 cut(s) 130
BsoBI CYCGRG 1 cut(s) 112
Bsp119I TTCGAA 1 cut(s) 681
Bsp143I GATC 4 cut(s) 3, 368, 786, 862
BspACI CCGC 3 cut(s) 393, 492, 859
BspANI GGCC 2 cut(s) 101, 496
BspLI GGNNCC 3 cut(s) 229, 883, 884
BspMI ACCTGC 1 cut(s) 217
BspPI GGATC 3 cut(s) 363, 794, 870
BspT104I TTCGAA 1 cut(s) 681
BspTNI GGTCTC 1 cut(s) 130
BssECI CCNNGG 4 cut(s) 92, 306, 600, 673
BssMI GATC 4 cut(s) 3, 368, 786, 862
BssT1I CCWWGG 1 cut(s) 673
Bst2UI CCWGG 1 cut(s) 601
Bst4CI ACNGT 5 cut(s) 68, 194, 346, 665, 878
BstBI TTCGAA 1 cut(s) 681
BstC8I GCNNGC 2 cut(s) 20, 494
BstDEI CTNAG 2 cut(s) 251, 563
BstDSI CCRYGG 1 cut(s) 306
BstEII GGTNACC 1 cut(s) 163
BstF5I GGATG 4 cut(s) 302, 519, 663, 714
BstH2I RGCGCY 2 cut(s) 243, 559
BstHHI GCGC 2 cut(s) 242, 558
BstKTI GATC 4 cut(s) 6, 371, 789, 865
BstMAI GTCTC 4 cut(s) 72, 130, 321, 602
BstMBI GATC 4 cut(s) 3, 368, 786, 862
BstMCI CGRYCG 1 cut(s) 348
BstNI CCWGG 1 cut(s) 601
BstNSI RCATGY 3 cut(s) 586, 840, 859
BstPAI GACNNNNGTC 1 cut(s) 839
BstPI GGTNACC 1 cut(s) 163
BstSCI CCNGG 2 cut(s) 91, 599
BstV1I GCAGC 1 cut(s) 70
BstV2I GAAGAC 1 cut(s) 835
BstX2I RGATCY 2 cut(s) 368, 786
BstYI RGATCY 2 cut(s) 368, 786
BsuRI GGCC 2 cut(s) 101, 496
BtgI CCRYGG 1 cut(s) 306
BtrI CACGTC 1 cut(s) 474
BtsCI GGATG 4 cut(s) 302, 519, 663, 714
BtsIMutI CAGTG 1 cut(s) 64
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 2 cut(s) 20, 494
CfoI GCGC 2 cut(s) 242, 558
Cfr13I GGNCC 4 cut(s) 100, 227, 494, 882
CseI GACGC 1 cut(s) 420
Csp6I GTAC 1 cut(s) 712
CviAII CATG 6 cut(s) 52, 583, 751, 837, 856, 903
CviJI RGCY 9 cut(s) 39, 61, 101, 117, 400, 450, 496, 647, 678
CviKI_1 RGCY 9 cut(s) 39, 61, 101, 117, 400, 450, 496, 647, 678
CviQI GTAC 1 cut(s) 712
DdeI CTNAG 2 cut(s) 251, 563
DpnI GATC 4 cut(s) 5, 370, 788, 864
DpnII GATC 4 cut(s) 3, 368, 786, 862
DraIII CACNNNGTG 1 cut(s) 871
DriI GACNNNNNGTC 1 cut(s) 233
Eam1105I GACNNNNNGTC 1 cut(s) 233
EciI GGCGGA 1 cut(s) 408
Eco130I CCWWGG 1 cut(s) 673
Eco31I GGTCTC 1 cut(s) 130
Eco47I GGWCC 2 cut(s) 227, 882
Eco88I CYCGRG 1 cut(s) 112
Eco91I GGTNACC 1 cut(s) 163
EcoO109I RGGNCCY 2 cut(s) 100, 882
EcoO65I GGTNACC 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 599
EcoT14I CCWWGG 1 cut(s) 673
EcoT22I ATGCAT 1 cut(s) 904
ErhI CCWWGG 1 cut(s) 673
FaeI CATG 6 cut(s) 55, 586, 754, 840, 859, 906
FaqI GGGAC 3 cut(s) 484, 847, 868
FatI CATG 6 cut(s) 51, 582, 750, 836, 855, 902
FauI CCCGC 2 cut(s) 485, 852
FauNDI CATATG 1 cut(s) 316
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 59
FokI GGATG 4 cut(s) 289, 506, 670, 721
Fsp4HI GCNGC 1 cut(s) 59
FspBI CTAG 1 cut(s) 383
GlaI GCGC 2 cut(s) 241, 557
GluI GCNGC 1 cut(s) 59
HaeII RGCGCY 2 cut(s) 243, 559
HaeIII GGCC 2 cut(s) 101, 496
HapII CCGG 1 cut(s) 92
HgaI GACGC 1 cut(s) 420
HhaI GCGC 2 cut(s) 242, 558
Hin1II CATG 6 cut(s) 55, 586, 754, 840, 859, 906
Hin6I GCGC 2 cut(s) 240, 556
HinP1I GCGC 2 cut(s) 240, 556
HindIII AAGCTT 2 cut(s) 37, 398
HinfI GANTC 1 cut(s) 297
HpaII CCGG 1 cut(s) 92
HphI GGTGA 2 cut(s) 239, 451
Hpy166II GTNNAC 3 cut(s) 144, 412, 874
Hpy188I TCNGA 5 cut(s) 44, 220, 227, 702, 769
Hpy188III TCNNGA 2 cut(s) 793, 847
Hpy8I GTNNAC 3 cut(s) 144, 412, 874
Hpy99I CGWCG 2 cut(s) 239, 719
HpyAV CCTTC 1 cut(s) 241
HpyCH4III ACNGT 5 cut(s) 68, 194, 346, 665, 878
HpyCH4IV ACGT 3 cut(s) 146, 473, 714
HpyCH4V TGCA 6 cut(s) 58, 181, 212, 276, 532, 902
HpyF3I CTNAG 2 cut(s) 251, 563
HpySE526I ACGT 3 cut(s) 146, 473, 714
Hsp92II CATG 6 cut(s) 55, 586, 754, 840, 859, 906
HspAI GCGC 2 cut(s) 240, 556
KflI GGGWCCC 1 cut(s) 882
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 368, 786, 862
LmnI GCTCC 1 cut(s) 563
LpnPI CCDG 6 cut(s) 105, 222, 286, 586, 613, 664
Lsp1109I GCAGC 1 cut(s) 70
MaeI CTAG 1 cut(s) 383
MaeII ACGT 3 cut(s) 146, 473, 714
MaeIII GTNAC 4 cut(s) 163, 338, 432, 665
MalI GATC 4 cut(s) 5, 370, 788, 864
MboI GATC 4 cut(s) 3, 368, 786, 862
MboII GAAGA 4 cut(s) 781, 835, 835, 862
MflI RGATCY 2 cut(s) 368, 786
MluCI AATT 6 cut(s) 106, 278, 592, 613, 683, 762
MmeI TCCRAC 1 cut(s) 635
MnlI CCTC 8 cut(s) 91, 113, 121, 169, 178, 382, 598, 777
Mph1103I ATGCAT 1 cut(s) 904
MseI TTAA 2 cut(s) 87, 729
MslI CAYNNNNRTG 1 cut(s) 524
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 2 cut(s) 93, 601
MvaI CCWGG 1 cut(s) 601
NciI CCSGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 316
NdeII GATC 4 cut(s) 3, 368, 786, 862
NlaIII CATG 6 cut(s) 55, 586, 754, 840, 859, 906
NlaIV GGNNCC 3 cut(s) 229, 883, 884
NmuCI GTSAC 3 cut(s) 338, 432, 665
NsiI ATGCAT 1 cut(s) 904
NspI RCATGY 3 cut(s) 586, 840, 859
NspV TTCGAA 1 cut(s) 681
PaeR7I CTCGAG 1 cut(s) 112
PciI ACATGT 2 cut(s) 582, 836
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PfeI GAWTC 1 cut(s) 297
PkrI GCNGC 1 cut(s) 60
PpuMI RGGWCCY 1 cut(s) 882
PscI ACATGT 2 cut(s) 582, 836
PshAI GACNNNNGTC 1 cut(s) 839
Psp5II RGGWCCY 1 cut(s) 882
Psp6I CCWGG 1 cut(s) 599
PspEI GGTNACC 1 cut(s) 163
PspGI CCWGG 1 cut(s) 599
PspN4I GGNNCC 3 cut(s) 229, 883, 884
PspPI GGNCC 4 cut(s) 100, 227, 494, 882
PspPPI RGGWCCY 1 cut(s) 882
PspXI VCTCGAGB 1 cut(s) 112
PsuI RGATCY 2 cut(s) 368, 786
RsaI GTAC 1 cut(s) 713
RsaNI GTAC 1 cut(s) 712
RseI CAYNNNNRTG 1 cut(s) 524
SaqAI TTAA 2 cut(s) 87, 729
SatI GCNGC 1 cut(s) 59
Sau3AI GATC 4 cut(s) 3, 368, 786, 862
Sau96I GGNCC 4 cut(s) 100, 227, 494, 882
ScrFI CCNGG 2 cut(s) 93, 601
Sfr274I CTCGAG 1 cut(s) 112
SfuI TTCGAA 1 cut(s) 681
SinI GGWCC 2 cut(s) 227, 882
SlaI CTCGAG 1 cut(s) 112
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 2 cut(s) 34, 112
SmoI CTYRAG 2 cut(s) 34, 112
Sse9I AATT 6 cut(s) 106, 278, 592, 613, 683, 762
SsiI CCGC 3 cut(s) 393, 492, 859
SspMI CTAG 1 cut(s) 383
StyD4I CCNGG 2 cut(s) 91, 599
StyI CCWWGG 1 cut(s) 673
TaaI ACNGT 5 cut(s) 68, 194, 346, 665, 878
TaiI ACGT 3 cut(s) 149, 476, 717
TaqI TCGA 5 cut(s) 113, 174, 622, 681, 717
TaqII GACCGA 1 cut(s) 326
TasI AATT 6 cut(s) 106, 278, 592, 613, 683, 762
TfiI GAWTC 1 cut(s) 297
Tru1I TTAA 2 cut(s) 87, 729
Tru9I TTAA 2 cut(s) 87, 729
TscAI CASTG 1 cut(s) 71
TseFI GTSAC 3 cut(s) 338, 432, 665
TseI GCWGC 1 cut(s) 58
Tsp45I GTSAC 3 cut(s) 338, 432, 665
TspDTI ATGAA 8 cut(s) 60, 303, 593, 651, 759, 767, 816, 836
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 1 cut(s) 71
VpaK11BI GGWCC 2 cut(s) 227, 882
XapI RAATTY 3 cut(s) 613, 683, 762
XceI RCATGY 3 cut(s) 586, 840, 859
XhoI CTCGAG 1 cut(s) 112
XspI CTAG 1 cut(s) 383
Zsp2I ATGCAT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.