Rroxscaffold_1G00071430

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
N/A
Physical Location & Seq
Reverse (-)
92249387 .. 92250929
1543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00071430.1

Sequence Viewer

Length: 720 bp
ATGCAGCTGCTGGGACAAACTATGAACAGCTTGGTGGTTCCTGCTGCTAATGCACCTTCAGGTGCGACAAAATTAGCAACAAAAGAAGTGAACTTCTCAGATTTTCAGCTGTTGTACACCCTTGTGCAGTGCACACCAGACCTGTCGAGCCAAAGTTGTGAACGTTGTCTTCGAGGAGCTATTGGAGTTCTTCCAGTTTGTTGTCATGGAAAGCAAGGGGGAAATGTCTTATTTCCTAGTTGCGATATTCGACATGAAATCTATCCATTTTACACAGCTCAAGCTGCCACACCTCAACCAGCGTCATCGCCACCATCACTGCTTCTTCCTCCCCCTCCTCCAGGTCCAGTAACGAGATCTCAAGAGTCTTTGCAGTTTGATTTGGCTTCCATTGAAGCAGCCACAAACCGTTTCTCTGTTAATAACAAGTTAGGCGAAGGTAGCTTTGGAGAGGTCTATAAGGGCACACTACCTAATGGGCAAGAGATAGCTGTGAAGAGGCTATCTAGAAGCTCCGGGCAAAGTGTCATGGAGTTTAAGATTGAGGTTGTTTTGGTAGCCAAGCTTCAGCACAGAAATTTAGTGAGGTTGCTGGGGTTTTGCTCTGAAGGAGAAGAAAAGATACTTGTTTTTGAACTTGTGCAGAACAGAAGCCTGGACCACTTTTTATTAGGTATGTGCCTTGTAAGATATATAATCATCAAGGGTGAATTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

239

Amino Acids

26.05

Weight (kDa)

6.89

Isoelectric Point (pI)

59.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 27 - 86 2.1e-07 Salt stress response/antifungal
Pkinase PF00069 138 - 223 6.6e-14 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 140 - 223 1.3e-16 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 163
AcsI RAATTY 2 cut(s) 577, 710
AcuI CTGAAG 3 cut(s) 42, 551, 627
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 341
AgsI TTSAA 2 cut(s) 395, 635
AjnI CCWGG 2 cut(s) 340, 654
AjuI GAANNNNNNNTTGG 2 cut(s) 429, 461
AleI CACNNNNGTG 1 cut(s) 122
Alw21I GWGCWC 1 cut(s) 134
Alw44I GTGCAC 1 cut(s) 130
AlwNI CAGNNNCTG 1 cut(s) 10
ApaLI GTGCAC 1 cut(s) 130
ApeKI GCWGC 5 cut(s) 4, 7, 44, 284, 398
ApoI RAATTY 2 cut(s) 577, 710
AspS9I GGNCC 2 cut(s) 344, 658
AsuC2I CCSGG 1 cut(s) 517
AsuHPI GGTGA 1 cut(s) 719
AvaII GGWCC 2 cut(s) 344, 658
BaeGI GKGCMC 2 cut(s) 134, 467
BarI GAAGNNNNNNTAC 4 cut(s) 549, 581, 606, 638
BbsI GAAGAC 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 4 cut(s) 16, 31, 271, 410
BccI CCATC 1 cut(s) 322
BciT130I CCWGG 2 cut(s) 342, 656
BcnI CCSGG 1 cut(s) 517
BfaI CTAG 2 cut(s) 237, 507
BglII AGATCT 1 cut(s) 356
BisI GCNGC 5 cut(s) 5, 8, 45, 285, 399
BlsI GCNGC 5 cut(s) 6, 9, 46, 286, 400
Bme1390I CCNGG 3 cut(s) 342, 517, 656
Bme18I GGWCC 2 cut(s) 344, 658
BmgT120I GGNCC 2 cut(s) 344, 658
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 3 cut(s) 342, 517, 656
BpiI GAAGAC 1 cut(s) 161
BpmI CTGGAG 1 cut(s) 324
BpuEI CTTGAG 2 cut(s) 264, 345
BpuMI CCSGG 1 cut(s) 517
Bsc4I CCNNNNNNNGG 1 cut(s) 341
Bse1I ACTGG 2 cut(s) 194, 347
BseBI CCWGG 2 cut(s) 342, 656
BseLI CCNNNNNNNGG 1 cut(s) 341
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 2 cut(s) 194, 347
BseRI GAGGAG 2 cut(s) 189, 327
BseSI GKGCMC 2 cut(s) 134, 467
BseXI GCAGC 4 cut(s) 16, 31, 271, 410
BseYI CCCAGC 2 cut(s) 10, 592
BsgI GTGCAG 2 cut(s) 146, 662
BsiHKAI GWGCWC 1 cut(s) 134
BsiSI CCGG 1 cut(s) 516
BslFI GGGAC 1 cut(s) 27
BslI CCNNNNNNNGG 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 27
Bsp1286I GDGCHC 2 cut(s) 134, 467
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 1 cut(s) 356
BspCNI CTCAG 1 cut(s) 110
BspLI GGNNCC 1 cut(s) 39
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 2 cut(s) 194, 347
BssMI GATC 1 cut(s) 356
Bst2UI CCWGG 2 cut(s) 342, 656
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 1 cut(s) 491
BstAUI TGTACA 1 cut(s) 114
BstDEI CTNAG 1 cut(s) 97
BstENI CCTNNNNNAGG 1 cut(s) 339
BstKTI GATC 1 cut(s) 359
BstMBI GATC 1 cut(s) 356
BstMWI GCNNNNNNNGC 3 cut(s) 50, 284, 441
BstNI CCWGG 2 cut(s) 342, 656
BstSCI CCNGG 3 cut(s) 340, 515, 654
BstSLI GKGCMC 2 cut(s) 134, 467
BstV1I GCAGC 4 cut(s) 16, 31, 271, 410
BstV2I GAAGAC 1 cut(s) 161
BstX2I RGATCY 1 cut(s) 356
BstYI RGATCY 1 cut(s) 356
BtgZI GCGATG 1 cut(s) 291
BtsI GCAGTG 2 cut(s) 134, 317
BtsIMutI CAGTG 2 cut(s) 134, 317
CaiI CAGNNNCTG 1 cut(s) 10
Cfr13I GGNCC 2 cut(s) 344, 658
CseI GACGC 1 cut(s) 291
Csp6I GTAC 1 cut(s) 115
CviAII CATG 3 cut(s) 206, 254, 529
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 1 cut(s) 97
DpnI GATC 1 cut(s) 358
DpnII GATC 1 cut(s) 356
Eam1104I CTCTTC 1 cut(s) 491
EarI CTCTTC 1 cut(s) 491
Eco47I GGWCC 2 cut(s) 344, 658
Eco57I CTGAAG 3 cut(s) 42, 551, 627
EcoNI CCTNNNNNAGG 1 cut(s) 339
EcoRII CCWGG 2 cut(s) 340, 654
FaeI CATG 3 cut(s) 209, 257, 532
FaiI YATR 8 cut(s) 23, 207, 255, 459, 530, 677, 693, 695
FaqI GGGAC 1 cut(s) 27
FatI CATG 3 cut(s) 205, 253, 528
Fnu4HI GCNGC 5 cut(s) 5, 8, 45, 285, 399
Fsp4HI GCNGC 5 cut(s) 5, 8, 45, 285, 399
FspBI CTAG 2 cut(s) 237, 507
GluI GCNGC 5 cut(s) 5, 8, 45, 285, 399
GsaI CCCAGC 2 cut(s) 14, 596
GsuI CTGGAG 1 cut(s) 324
HapII CCGG 1 cut(s) 516
HgaI GACGC 1 cut(s) 291
Hin1II CATG 3 cut(s) 209, 257, 532
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 1 cut(s) 365
HpaII CCGG 1 cut(s) 516
HphI GGTGA 1 cut(s) 719
Hpy166II GTNNAC 4 cut(s) 91, 117, 132, 161
Hpy188I TCNGA 2 cut(s) 100, 607
Hpy188III TCNNGA 2 cut(s) 362, 507
Hpy8I GTNNAC 4 cut(s) 91, 117, 132, 161
HpyAV CCTTC 3 cut(s) 66, 431, 602
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 6 cut(s) 4, 53, 127, 132, 373, 643
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 284, 441
HpyF3I CTNAG 1 cut(s) 97
HpySE526I ACGT 1 cut(s) 163
Hsp92II CATG 3 cut(s) 209, 257, 532
Kzo9I GATC 1 cut(s) 356
LmnI GCTCC 2 cut(s) 176, 518
Lsp1109I GCAGC 4 cut(s) 16, 31, 271, 410
MaeI CTAG 2 cut(s) 237, 507
MaeII ACGT 1 cut(s) 163
MaeIII GTNAC 1 cut(s) 349
MalI GATC 1 cut(s) 358
MboI GATC 1 cut(s) 356
MboII GAAGA 5 cut(s) 161, 182, 317, 508, 626
MflI RGATCY 1 cut(s) 356
MhlI GDGCHC 2 cut(s) 134, 467
MluCI AATT 3 cut(s) 71, 577, 710
MlyI GAGTC 1 cut(s) 374
MnlI CCTC 9 cut(s) 167, 303, 339, 345, 348, 445, 492, 538, 579
MseI TTAA 3 cut(s) 420, 537, 718
MslI CAYNNNNRTG 1 cut(s) 122
MspA1I CMGCKG 2 cut(s) 7, 109
MspI CCGG 1 cut(s) 516
MspR9I CCNGG 3 cut(s) 342, 517, 656
MvaI CCWGG 2 cut(s) 342, 656
MwoI GCNNNNNNNGC 3 cut(s) 50, 284, 441
NciI CCSGG 1 cut(s) 517
NdeII GATC 1 cut(s) 356
NlaIII CATG 3 cut(s) 209, 257, 532
NlaIV GGNNCC 1 cut(s) 39
OliI CACNNNNGTG 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 169
PkrI GCNGC 5 cut(s) 6, 9, 46, 286, 400
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
Psp1406I AACGTT 1 cut(s) 163
Psp6I CCWGG 2 cut(s) 340, 654
PspFI CCCAGC 2 cut(s) 10, 592
PspGI CCWGG 2 cut(s) 340, 654
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 344, 658
PstNI CAGNNNCTG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 356
PvuII CAGCTG 2 cut(s) 7, 109
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 122
SaqAI TTAA 3 cut(s) 420, 537, 718
SatI GCNGC 5 cut(s) 5, 8, 45, 285, 399
Sau3AI GATC 1 cut(s) 356
Sau96I GGNCC 2 cut(s) 344, 658
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 3 cut(s) 342, 517, 656
SduI GDGCHC 2 cut(s) 134, 467
SinI GGWCC 2 cut(s) 344, 658
SmiMI CAYNNNNRTG 1 cut(s) 122
SmlI CTYRAG 2 cut(s) 279, 360
SmoI CTYRAG 2 cut(s) 279, 360
Sse9I AATT 3 cut(s) 71, 577, 710
SspMI CTAG 2 cut(s) 237, 507
StyD4I CCNGG 3 cut(s) 340, 515, 654
TaaI ACNGT 1 cut(s) 410
TaiI ACGT 1 cut(s) 166
TaqI TCGA 3 cut(s) 146, 172, 250
TasI AATT 3 cut(s) 71, 577, 710
TatI WGTACW 1 cut(s) 114
Tru1I TTAA 3 cut(s) 420, 537, 718
Tru9I TTAA 3 cut(s) 420, 537, 718
TscAI CASTG 2 cut(s) 134, 324
TseI GCWGC 5 cut(s) 4, 7, 44, 284, 398
TspDTI ATGAA 2 cut(s) 38, 270
TspRI CASTG 2 cut(s) 134, 324
VneI GTGCAC 1 cut(s) 130
VpaK11BI GGWCC 2 cut(s) 344, 658
XagI CCTNNNNNAGG 1 cut(s) 339
XapI RAATTY 2 cut(s) 577, 710
XbaI TCTAGA 1 cut(s) 506
XspI CTAG 2 cut(s) 237, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.