Rroxscaffold_1G00074810

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
N/A
Physical Location & Seq
Forward (+)
95661436 .. 95662644
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00074810.1

Sequence Viewer

Length: 732 bp
ATGGAAGACTCAACTGAGACTGGTAGTGATAAATGGGTCGTGTGCTCTCCCACAAATAAAAGGCCTGATTCTTTAAATCAGGCTGGGCCGAAGAATCAGAAAAAAATCTGGAAAGCGAGGTTTCTTGGTGCGCAAACACGCTGGTGGTGCTCTGATCAGGATAAGGAGAATAAACTCAAATTGGAAGGCAAGATGCTGTTGGAGTGGAAAAGCCTTCTCTTAATATACAATATGGTTGATCCTATTGATCTTCTGTGCAAAACAATCCTCACTTTTACCACAGATCCCAGGAAACGGTCTGAGAAGATAAATAGATGGGGGCTTTGGGGCGGAATCAGATTCAGTTGTAGCAATAGCTTAAGCCCAATCCTGTATTCACTTCAATTTTCAATACAAGCAGTGAAGAGGTCTTTTACACTTGAACTTATAGACAAGTCTACTTTATCAAGGTTTGTGATGAATCAAGACAGCTCAGTTGTATATTTTACTTGGAAAGCAAGCAACTTTCAGTGGGGTGGTGTGGTGGCACTTCAACAAAGCACTTTCGATGGGAATGGGATCTGTGGTCCTTATGGCAGTTGTGACTCCAAAGGCCCTAGTTGTGTGTGTTTGAAAGGATTTGCTCCCAAGTCACCAGAGGAATGGCAGATGCTCACTGGACTGGAGGCGATGCGCGAGAATGGGACTTGGATTGTAAGAATGGAGATGGCTTTGGTAGGTACAATGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

243

Amino Acids

27.58

Weight (kDa)

8.86

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 105 - 211 1.4e-16 S-locus glycoprotein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 132
AccI GTMKAC 1 cut(s) 437
AccII CGCG 1 cut(s) 675
AciI CCGC 1 cut(s) 330
AclWI GGATC 3 cut(s) 233, 278, 566
AfaI GTAC 1 cut(s) 721
AfiI CCNNNNNNNGG 1 cut(s) 294
AflII CTTAAG 1 cut(s) 358
AgsI TTSAA 5 cut(s) 383, 390, 422, 533, 613
AjnI CCWGG 1 cut(s) 287
AjuI GAANNNNNNNTTGG 2 cut(s) 358, 390
AleI CACNNNNGTG 1 cut(s) 142
AluBI AGCT 2 cut(s) 357, 471
AluI AGCT 2 cut(s) 357, 471
Alw21I GWGCWC 2 cut(s) 47, 152
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 3 cut(s) 233, 278, 566
AoxI GGCC 3 cut(s) 62, 86, 592
AspLEI GCGC 2 cut(s) 133, 675
AspS9I GGNCC 3 cut(s) 86, 566, 593
AsuHPI GGTGA 1 cut(s) 624
AvaII GGWCC 1 cut(s) 566
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 47, 152
BccI CCATC 3 cut(s) 309, 542, 700
BciT130I CCWGG 1 cut(s) 289
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 1 cut(s) 597
BfrI CTTAAG 1 cut(s) 358
Bme1390I CCNGG 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 566
BmgT120I GGNCC 3 cut(s) 86, 566, 593
BmrFI CCNGG 1 cut(s) 289
BmsI GCATC 3 cut(s) 183, 639, 660
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 1 cut(s) 683
BsaJI CCNNGG 1 cut(s) 287
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse1I ACTGG 3 cut(s) 25, 661, 666
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 1 cut(s) 287
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMII CTCAG 3 cut(s) 6, 291, 486
BseNI ACTGG 3 cut(s) 25, 661, 666
BseYI CCCAGC 1 cut(s) 83
Bsh1236I CGCG 1 cut(s) 675
BshFI GGCC 3 cut(s) 64, 88, 594
BsiHKAI GWGCWC 2 cut(s) 47, 152
BslFI GGGAC 1 cut(s) 697
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 697
BsnI GGCC 3 cut(s) 64, 88, 594
Bsp1286I GDGCHC 2 cut(s) 47, 152
Bsp143I GATC 5 cut(s) 154, 238, 247, 283, 558
BspACI CCGC 1 cut(s) 330
BspANI GGCC 3 cut(s) 64, 88, 594
BspCNI CTCAG 3 cut(s) 7, 292, 485
BspFNI CGCG 1 cut(s) 675
BspPI GGATC 3 cut(s) 233, 278, 566
BspTI CTTAAG 1 cut(s) 358
BsrI ACTGG 3 cut(s) 25, 661, 666
BssECI CCNNGG 1 cut(s) 287
BssMI GATC 5 cut(s) 154, 238, 247, 283, 558
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 1 cut(s) 297
Bst6I CTCTTC 1 cut(s) 398
BstAFI CTTAAG 1 cut(s) 358
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 3 cut(s) 15, 300, 472
BstFNI CGCG 1 cut(s) 675
BstHHI GCGC 2 cut(s) 133, 675
BstKTI GATC 5 cut(s) 157, 241, 250, 286, 561
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 5 cut(s) 154, 238, 247, 283, 558
BstMWI GCNNNNNNNGC 1 cut(s) 147
BstNI CCWGG 1 cut(s) 289
BstSCI CCNGG 1 cut(s) 287
BstUI CGCG 1 cut(s) 675
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 2 cut(s) 283, 558
BstXI CCANNNNNNTGG 1 cut(s) 642
BstYI RGATCY 2 cut(s) 283, 558
BsuRI GGCC 3 cut(s) 64, 88, 594
BtgZI GCGATG 1 cut(s) 683
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 3 cut(s) 405, 515, 654
Cac8I GCNNGC 1 cut(s) 499
CfoI GCGC 2 cut(s) 133, 675
Cfr13I GGNCC 3 cut(s) 86, 566, 593
Csp6I GTAC 1 cut(s) 720
CviQI GTAC 1 cut(s) 720
DdeI CTNAG 3 cut(s) 15, 300, 472
DpnI GATC 5 cut(s) 156, 240, 249, 285, 560
DpnII GATC 5 cut(s) 154, 238, 247, 283, 558
DraI TTTAAA 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
EciI GGCGGA 1 cut(s) 345
Eco147I AGGCCT 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 593
EcoRII CCWGG 1 cut(s) 287
FaiI YATR 5 cut(s) 226, 233, 428, 481, 573
FaqI GGGAC 1 cut(s) 697
FbaI TGATCA 1 cut(s) 154
FblI GTMKAC 1 cut(s) 437
FspBI CTAG 1 cut(s) 597
FspI TGCGCA 1 cut(s) 132
GlaI GCGC 2 cut(s) 132, 674
GsaI CCCAGC 1 cut(s) 87
GsuI CTGGAG 1 cut(s) 683
HaeIII GGCC 3 cut(s) 64, 88, 594
HhaI GCGC 2 cut(s) 133, 675
Hin6I GCGC 2 cut(s) 131, 673
HinP1I GCGC 2 cut(s) 131, 673
HinfI GANTC 7 cut(s) 8, 68, 94, 333, 339, 460, 584
HphI GGTGA 1 cut(s) 624
Hpy166II GTNNAC 1 cut(s) 438
Hpy188I TCNGA 4 cut(s) 99, 154, 301, 338
Hpy188III TCNNGA 3 cut(s) 109, 158, 464
Hpy8I GTNNAC 1 cut(s) 438
HpyAV CCTTC 2 cut(s) 179, 224
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4V TGCA 1 cut(s) 258
HpyF10VI GCNNNNNNNGC 1 cut(s) 147
HpyF3I CTNAG 3 cut(s) 15, 300, 472
HspAI GCGC 2 cut(s) 131, 673
Ksp22I TGATCA 1 cut(s) 154
Kzo9I GATC 5 cut(s) 154, 238, 247, 283, 558
LmnI GCTCC 1 cut(s) 628
LweI GCATC 3 cut(s) 183, 639, 660
MaeI CTAG 1 cut(s) 597
MaeIII GTNAC 2 cut(s) 581, 630
MalI GATC 5 cut(s) 156, 240, 249, 285, 560
MboI GATC 5 cut(s) 154, 238, 247, 283, 558
MboII GAAGA 5 cut(s) 17, 103, 242, 316, 415
MflI RGATCY 2 cut(s) 283, 558
MhlI GDGCHC 2 cut(s) 47, 152
MluCI AATT 2 cut(s) 179, 383
MlyI GAGTC 2 cut(s) 2, 578
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 5 cut(s) 111, 278, 399, 631, 658
MseI TTAA 3 cut(s) 74, 221, 359
MslI CAYNNNNRTG 1 cut(s) 142
MspCI CTTAAG 1 cut(s) 358
MspR9I CCNGG 1 cut(s) 289
MvaI CCWGG 1 cut(s) 289
MvnI CGCG 1 cut(s) 675
MwoI GCNNNNNNNGC 1 cut(s) 147
NdeII GATC 5 cut(s) 154, 238, 247, 283, 558
NmuCI GTSAC 2 cut(s) 581, 630
NsbI TGCGCA 1 cut(s) 132
OliI CACNNNNGTG 1 cut(s) 142
PceI AGGCCT 1 cut(s) 64
PfeI GAWTC 5 cut(s) 68, 94, 333, 339, 460
PleI GAGTC 2 cut(s) 2, 578
PpsI GAGTC 2 cut(s) 2, 578
Psp6I CCWGG 1 cut(s) 287
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 1 cut(s) 287
PspPI GGNCC 3 cut(s) 86, 566, 593
PsuI RGATCY 2 cut(s) 283, 558
RsaI GTAC 1 cut(s) 721
RsaNI GTAC 1 cut(s) 720
RseI CAYNNNNRTG 1 cut(s) 142
SaqAI TTAA 3 cut(s) 74, 221, 359
Sau3AI GATC 5 cut(s) 154, 238, 247, 283, 558
Sau96I GGNCC 3 cut(s) 86, 566, 593
SchI GAGTC 2 cut(s) 2, 578
ScrFI CCNGG 1 cut(s) 289
SduI GDGCHC 2 cut(s) 47, 152
SetI ASST 6 cut(s) 122, 359, 410, 452, 473, 721
SfaNI GCATC 3 cut(s) 183, 639, 660
SinI GGWCC 1 cut(s) 566
SmiMI CAYNNNNRTG 1 cut(s) 142
SmlI CTYRAG 1 cut(s) 358
SmoI CTYRAG 1 cut(s) 358
Sse9I AATT 2 cut(s) 179, 383
SseBI AGGCCT 1 cut(s) 64
SsiI CCGC 1 cut(s) 330
SspMI CTAG 1 cut(s) 597
StuI AGGCCT 1 cut(s) 64
StyD4I CCNGG 1 cut(s) 287
TaaI ACNGT 1 cut(s) 297
TaqI TCGA 1 cut(s) 546
TasI AATT 2 cut(s) 179, 383
TfiI GAWTC 5 cut(s) 68, 94, 333, 339, 460
Tru1I TTAA 3 cut(s) 74, 221, 359
Tru9I TTAA 3 cut(s) 74, 221, 359
TscAI CASTG 3 cut(s) 405, 515, 661
TseFI GTSAC 2 cut(s) 581, 630
Tsp45I GTSAC 2 cut(s) 581, 630
TspDTI ATGAA 1 cut(s) 473
TspRI CASTG 3 cut(s) 405, 515, 661
Vha464I CTTAAG 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 437
XspI CTAG 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.