Rroxscaffold_2G00085000

receptor-like protein kinase At1g67000

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
N/A
Physical Location & Seq
Forward (+)
7431583 .. 7433858
2276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00085000.1

Sequence Viewer

Length: 489 bp
ATGCCGGCTCTCGCCATCTCCGCCTCGAAACTCATCGTCGTGCTCTCTCGCGGCAAGGCTCGACCCCAAGCCGACGGTTCTCCAAGTTCACTTTCACCTGGTCCTGTCACGGGTTCTCCGAGTTCACTTTCACCTGGTCTTGTCGCAGGTTCTACGAGTTCACCTTCACCTGGTCCTGTCGCAGGTTCTCCGAGTTCACTTTCACCTGGTCTAGTCACGGGTTCTCCGAGTTCACTTTCACCTAGTCTTGTCGCAGGCATTTCGGCTGGTGTGGCTGGGACAATCATAGTTGCTGTCGGTATTATATACATATCAAGAAAAAGAATGGGCCTCCACAAGAAATTTACGAGGGATGAGTTTGATATCGAGGAATTCATAAGGAATAATGGATCGCTTACCCCTAAGCGGTATACTTATTCAAAGGTGAAGAAAATGACAAATGCATTCAAAGATAAAATAGGGAAAGTGTTGAAGGAAAATCAAGTTTAG

Protein Analysis

162

Amino Acids

16.61

Weight (kDa)

10.32

Isoelectric Point (pI)

59.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 137, 173
AccI GTMKAC 1 cut(s) 410
AccII CGCG 1 cut(s) 51
AciI CCGC 3 cut(s) 21, 51, 406
AclWI GGATC 1 cut(s) 397
AcsI RAATTY 2 cut(s) 341, 371
AfiI CCNNNNNNNGG 4 cut(s) 110, 170, 182, 405
AgsI TTSAA 3 cut(s) 420, 448, 472
AjnI CCWGG 4 cut(s) 97, 133, 169, 205
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
Alw21I GWGCWC 1 cut(s) 45
AlwI GGATC 1 cut(s) 397
AoxI GGCC 1 cut(s) 328
ApoI RAATTY 2 cut(s) 341, 371
AspS9I GGNCC 3 cut(s) 101, 173, 328
AsuHPI GGTGA 7 cut(s) 87, 123, 153, 159, 195, 231, 436
AvaII GGWCC 2 cut(s) 101, 173
Bbv12I GWGCWC 1 cut(s) 45
BccI CCATC 1 cut(s) 23
BcgI CGANNNNNNTGC 2 cut(s) 243, 277
BciT130I CCWGG 4 cut(s) 99, 135, 171, 207
BfaI CTAG 2 cut(s) 212, 243
BfuAI ACCTGC 2 cut(s) 137, 173
BisI GCNGC 1 cut(s) 52
BlsI GCNGC 1 cut(s) 53
Bme1390I CCNGG 4 cut(s) 99, 135, 171, 207
Bme18I GGWCC 2 cut(s) 101, 173
BmgT120I GGNCC 3 cut(s) 101, 173, 328
BmrFI CCNGG 4 cut(s) 99, 135, 171, 207
Bpu10I CCTNAGC 1 cut(s) 402
BsaXI ACNNNNNCTCC 4 cut(s) 100, 130, 208, 238
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 170, 182, 405
Bse118I RCCGGY 1 cut(s) 4
BseBI CCWGG 4 cut(s) 99, 135, 171, 207
BseGI GGATG 1 cut(s) 358
BseLI CCNNNNNNNGG 4 cut(s) 110, 170, 182, 405
BseYI CCCAGC 1 cut(s) 275
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 1 cut(s) 330
BsiHKAI GWGCWC 1 cut(s) 45
BsiSI CCGG 1 cut(s) 5
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 4 cut(s) 110, 170, 182, 405
BsmFI GGGAC 1 cut(s) 292
BsmI GAATGC 1 cut(s) 443
BsnI GGCC 1 cut(s) 330
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 1 cut(s) 389
BspACI CCGC 3 cut(s) 21, 51, 406
BspANI GGCC 1 cut(s) 330
BspFNI CGCG 1 cut(s) 51
BspMI ACCTGC 2 cut(s) 137, 173
BspPI GGATC 1 cut(s) 397
BsrFI RCCGGY 1 cut(s) 4
BssAI RCCGGY 1 cut(s) 4
BssMI GATC 1 cut(s) 389
BssNAI GTATAC 1 cut(s) 411
Bst1107I GTATAC 1 cut(s) 411
Bst2UI CCWGG 4 cut(s) 99, 135, 171, 207
Bst4CI ACNGT 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 6, 256
BstDEI CTNAG 1 cut(s) 402
BstENI CCTNNNNNAGG 1 cut(s) 180
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 1 cut(s) 51
BstKTI GATC 1 cut(s) 392
BstMBI GATC 1 cut(s) 389
BstMWI GCNNNNNNNGC 2 cut(s) 20, 272
BstNI CCWGG 4 cut(s) 99, 135, 171, 207
BstSCI CCNGG 4 cut(s) 97, 133, 169, 205
BstUI CGCG 1 cut(s) 51
BstZ17I GTATAC 1 cut(s) 411
BsuRI GGCC 1 cut(s) 330
BtsCI GGATG 1 cut(s) 358
BveI ACCTGC 2 cut(s) 137, 173
Cac8I GCNNGC 2 cut(s) 6, 256
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 3 cut(s) 101, 173, 328
CsiI ACCWGGT 4 cut(s) 97, 133, 169, 205
CviJI RGCY 6 cut(s) 8, 59, 71, 266, 275, 330
CviKI_1 RGCY 6 cut(s) 8, 59, 71, 266, 275, 330
DdeI CTNAG 1 cut(s) 402
DpnI GATC 1 cut(s) 391
DpnII GATC 1 cut(s) 389
EciI GGCGGA 1 cut(s) 10
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 101, 173
EcoNI CCTNNNNNAGG 1 cut(s) 180
EcoRI GAATTC 1 cut(s) 371
EcoRII CCWGG 4 cut(s) 97, 133, 169, 205
EcoRV GATATC 1 cut(s) 364
EcoT22I ATGCAT 1 cut(s) 445
FaiI YATR 6 cut(s) 287, 305, 307, 311, 377, 411
FaqI GGGAC 1 cut(s) 292
FblI GTMKAC 1 cut(s) 410
Fnu4HI GCNGC 1 cut(s) 52
FokI GGATG 1 cut(s) 365
Fsp4HI GCNGC 1 cut(s) 52
FspBI CTAG 2 cut(s) 212, 243
GluI GCNGC 1 cut(s) 52
GsaI CCCAGC 1 cut(s) 279
HaeIII GGCC 1 cut(s) 330
HapII CCGG 1 cut(s) 5
HpaII CCGG 1 cut(s) 5
HphI GGTGA 7 cut(s) 87, 123, 153, 159, 195, 231, 436
Hpy166II GTNNAC 6 cut(s) 89, 125, 161, 197, 233, 411
Hpy188I TCNGA 3 cut(s) 120, 192, 228
Hpy188III TCNNGA 1 cut(s) 315
Hpy8I GTNNAC 6 cut(s) 89, 125, 161, 197, 233, 411
Hpy99I CGWCG 2 cut(s) 41, 77
HpyAV CCTTC 2 cut(s) 174, 466
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4V TGCA 1 cut(s) 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 272
HpyF3I CTNAG 1 cut(s) 402
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 1 cut(s) 389
MabI ACCWGGT 4 cut(s) 97, 133, 169, 205
MaeI CTAG 2 cut(s) 212, 243
MaeIII GTNAC 2 cut(s) 106, 214
MalI GATC 1 cut(s) 391
MboI GATC 1 cut(s) 389
MboII GAAGA 1 cut(s) 439
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 2 cut(s) 341, 371
MnlI CCTC 4 cut(s) 34, 341, 342, 361
Mph1103I ATGCAT 1 cut(s) 445
MroNI GCCGGC 1 cut(s) 4
MslI CAYNNNNRTG 1 cut(s) 38
MspI CCGG 1 cut(s) 5
MspR9I CCNGG 4 cut(s) 99, 135, 171, 207
Mva1269I GAATGC 1 cut(s) 443
MvaI CCWGG 4 cut(s) 99, 135, 171, 207
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 2 cut(s) 20, 272
NaeI GCCGGC 1 cut(s) 6
NdeII GATC 1 cut(s) 389
NgoMIV GCCGGC 1 cut(s) 4
NmuCI GTSAC 2 cut(s) 106, 214
NsiI ATGCAT 1 cut(s) 445
PcsI WCGNNNNNNNCGW 2 cut(s) 116, 224
PctI GAATGC 1 cut(s) 443
PdiI GCCGGC 1 cut(s) 6
PkrI GCNGC 1 cut(s) 53
Psp6I CCWGG 4 cut(s) 97, 133, 169, 205
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 4 cut(s) 97, 133, 169, 205
PspPI GGNCC 3 cut(s) 101, 173, 328
RseI CAYNNNNRTG 1 cut(s) 38
SatI GCNGC 1 cut(s) 52
Sau3AI GATC 1 cut(s) 389
Sau96I GGNCC 3 cut(s) 101, 173, 328
ScrFI CCNGG 4 cut(s) 99, 135, 171, 207
SduI GDGCHC 1 cut(s) 45
SetI ASST 9 cut(s) 100, 136, 151, 166, 172, 187, 208, 244, 426
SexAI ACCWGGT 4 cut(s) 97, 133, 169, 205
SinI GGWCC 2 cut(s) 101, 173
SmiMI CAYNNNNRTG 1 cut(s) 38
Sse9I AATT 2 cut(s) 341, 371
SsiI CCGC 3 cut(s) 21, 51, 406
SspMI CTAG 2 cut(s) 212, 243
StyD4I CCNGG 4 cut(s) 97, 133, 169, 205
TaaI ACNGT 1 cut(s) 77
TaqI TCGA 3 cut(s) 26, 61, 366
TasI AATT 2 cut(s) 341, 371
TauI GCSGC 1 cut(s) 54
TseFI GTSAC 2 cut(s) 106, 214
Tsp45I GTSAC 2 cut(s) 106, 214
TspDTI ATGAA 1 cut(s) 364
VpaK11BI GGWCC 2 cut(s) 101, 173
XagI CCTNNNNNAGG 1 cut(s) 180
XapI RAATTY 2 cut(s) 341, 371
XmiI GTMKAC 1 cut(s) 410
XspI CTAG 2 cut(s) 212, 243
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.