Rroxscaffold_2G00126750

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
N/A
Physical Location & Seq
Forward (+)
62120890 .. 62134437
13548 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126750.1

Sequence Viewer

Length: 534 bp
ATGCTGGGAATCTTACATAGATTTTGTGTTTGGGGGATCAATGCCTTCATAGATAATGATCTTGCTAGAGGAGAAGAAATATCGGGAGAGCTTCTCAAAACAATTGAGGATTCAAGGATTTCTGTCATCATATTCTCTGAAAACTATGCATCGTCAAAGTGGTGTTTGGACGAACTTGTTAAGATCCTTGAATGTGGATCCATCGATGTGCGGAACCAAAGAGGTAGTTTTGGTGAGGCAATTGTTCAGCATGAACACAAGTTTAATGATGACATTGAGAAAGTAAAGAGATGGAGGGCAGCTCTTTCAAAAGCAGCAGCTTTGTCGTGCAACCATTTCTTTGACGGTTATGAATCCAAATTTATCCATGAGGTGGTTGAGGAGATTTCGGTCAAGGTATTGCGCCGCACATATTTGAATGTGGCTAAGCATCCGGTTGGGATACAGCATCGTCAACAATGTGTCGAAGAGCTCTTAGGTGTTGGGGAAGATGATGTTCGAATGGTAGGGATATGGGGCACACCGGTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

177

Amino Acids

20.16

Weight (kDa)

5.53

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 4 - 133 2.3e-29 TIR domain
TIR_2 PF13676 12 - 64 7.8e-07 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 373
AciI CCGC 2 cut(s) 211, 406
AclWI GGATC 4 cut(s) 44, 178, 192, 205
AcsI RAATTY 1 cut(s) 359
AfiI CCNNNNNNNGG 1 cut(s) 373
AgeI ACCGGT 1 cut(s) 523
AgsI TTSAA 4 cut(s) 114, 191, 309, 418
AleI CACNNNNGTG 1 cut(s) 524
AluBI AGCT 4 cut(s) 91, 302, 320, 472
AluI AGCT 4 cut(s) 91, 302, 320, 472
Alw21I GWGCWC 1 cut(s) 474
AlwI GGATC 4 cut(s) 44, 178, 192, 205
ApeKI GCWGC 3 cut(s) 299, 314, 317
ApoI RAATTY 1 cut(s) 359
AsiGI ACCGGT 1 cut(s) 523
AspLEI GCGC 1 cut(s) 405
AsuHPI GGTGA 1 cut(s) 245
AsuII TTCGAA 1 cut(s) 499
BaeGI GKGCMC 1 cut(s) 521
BamHI GGATCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 474
Bbv12I GWGCWC 1 cut(s) 474
BbvI GCAGC 3 cut(s) 311, 326, 329
BccI CCATC 2 cut(s) 209, 285
BcgI CGANNNNNNTGC 2 cut(s) 306, 340
BciVI GTATCC 1 cut(s) 435
BfaI CTAG 1 cut(s) 66
BfuI GTATCC 1 cut(s) 435
BisI GCNGC 4 cut(s) 300, 315, 318, 406
BlpI GCTNAGC 1 cut(s) 426
BlsI GCNGC 4 cut(s) 301, 316, 319, 407
BmiI GGNNCC 2 cut(s) 199, 215
BmsI GCATC 3 cut(s) 158, 439, 457
BplI GAGNNNNNCTC 4 cut(s) 78, 110, 286, 318
Bpu1102I GCTNAGC 1 cut(s) 426
Bpu14I TTCGAA 1 cut(s) 499
Bsa29I ATCGAT 1 cut(s) 204
BsaBI GATNNNNATC 1 cut(s) 57
BsaWI WCCGGW 2 cut(s) 433, 523
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse118I RCCGGY 1 cut(s) 523
Bse8I GATNNNNATC 1 cut(s) 57
BseCI ATCGAT 1 cut(s) 204
BseGI GGATG 1 cut(s) 430
BseJI GATNNNNATC 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 373
BseRI GAGGAG 2 cut(s) 84, 395
BseSI GKGCMC 1 cut(s) 521
BseXI GCAGC 3 cut(s) 311, 326, 329
BseYI CCCAGC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 523
BshVI ATCGAT 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 474
BsiSI CCGG 2 cut(s) 434, 524
BslI CCNNNNNNNGG 1 cut(s) 373
Bsp119I TTCGAA 1 cut(s) 499
Bsp1286I GDGCHC 2 cut(s) 474, 521
Bsp143I GATC 4 cut(s) 36, 58, 183, 197
Bsp1720I GCTNAGC 1 cut(s) 426
BspACI CCGC 2 cut(s) 211, 406
BspDI ATCGAT 1 cut(s) 204
BspLI GGNNCC 2 cut(s) 199, 215
BspPI GGATC 4 cut(s) 44, 178, 192, 205
BspQI GCTCTTC 1 cut(s) 462
BspT104I TTCGAA 1 cut(s) 499
BsrFI RCCGGY 1 cut(s) 523
BssAI RCCGGY 1 cut(s) 523
BssMI GATC 4 cut(s) 36, 58, 183, 197
Bst4CI ACNGT 1 cut(s) 347
Bst6I CTCTTC 1 cut(s) 462
BstBI TTCGAA 1 cut(s) 499
BstDEI CTNAG 2 cut(s) 426, 475
BstF5I GGATG 1 cut(s) 430
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 4 cut(s) 39, 61, 186, 200
BstMBI GATC 4 cut(s) 36, 58, 183, 197
BstSLI GKGCMC 1 cut(s) 521
BstV1I GCAGC 3 cut(s) 311, 326, 329
BstX2I RGATCY 2 cut(s) 183, 197
BstYI RGATCY 2 cut(s) 183, 197
Bsu15I ATCGAT 1 cut(s) 204
BsuI GTATCC 1 cut(s) 435
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 1 cut(s) 430
CfoI GCGC 1 cut(s) 405
Cfr10I RCCGGY 1 cut(s) 523
ClaI ATCGAT 1 cut(s) 204
CspAI ACCGGT 1 cut(s) 523
CviAII CATG 2 cut(s) 251, 368
CviJI RGCY 5 cut(s) 91, 302, 320, 425, 472
CviKI_1 RGCY 5 cut(s) 91, 302, 320, 425, 472
DdeI CTNAG 2 cut(s) 426, 475
DpnI GATC 4 cut(s) 38, 60, 185, 199
DpnII GATC 4 cut(s) 36, 58, 183, 197
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Ecl136II GAGCTC 1 cut(s) 472
Eco24I GRGCYC 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 472
EcoT22I ATGCAT 1 cut(s) 151
EcoT38I GRGCYC 1 cut(s) 474
FaeI CATG 2 cut(s) 254, 371
FaiI YATR 9 cut(s) 18, 50, 131, 147, 252, 351, 369, 412, 514
FatI CATG 2 cut(s) 250, 367
Fnu4HI GCNGC 4 cut(s) 300, 315, 318, 406
FokI GGATG 1 cut(s) 417
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 4 cut(s) 300, 315, 318, 406
FspBI CTAG 1 cut(s) 66
GlaI GCGC 1 cut(s) 404
GluI GCNGC 4 cut(s) 300, 315, 318, 406
GsaI CCCAGC 1 cut(s) 8
HapII CCGG 2 cut(s) 434, 524
HhaI GCGC 1 cut(s) 405
Hin1II CATG 2 cut(s) 254, 371
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HincII GTYRAC 1 cut(s) 455
HindII GTYRAC 1 cut(s) 455
HinfI GANTC 3 cut(s) 9, 110, 353
HpaII CCGG 2 cut(s) 434, 524
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 1 cut(s) 455
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 1 cut(s) 84
Hpy8I GTNNAC 1 cut(s) 455
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4V TGCA 2 cut(s) 149, 330
HpyF3I CTNAG 2 cut(s) 426, 475
Hsp92II CATG 2 cut(s) 254, 371
HspAI GCGC 1 cut(s) 403
Kzo9I GATC 4 cut(s) 36, 58, 183, 197
LguI GCTCTTC 1 cut(s) 462
LpnPI CCDG 1 cut(s) 447
Lsp1109I GCAGC 3 cut(s) 311, 326, 329
LweI GCATC 3 cut(s) 158, 439, 457
MaeI CTAG 1 cut(s) 66
MalI GATC 4 cut(s) 38, 60, 185, 199
MboI GATC 4 cut(s) 36, 58, 183, 197
MboII GAAGA 3 cut(s) 86, 479, 500
MfeI CAATTG 2 cut(s) 102, 240
MflI RGATCY 2 cut(s) 183, 197
MhlI GDGCHC 2 cut(s) 474, 521
MluCI AATT 3 cut(s) 102, 240, 359
MnlI CCTC 7 cut(s) 62, 100, 215, 229, 288, 364, 373
Mph1103I ATGCAT 1 cut(s) 151
MseI TTAA 2 cut(s) 180, 264
MslI CAYNNNNRTG 2 cut(s) 206, 524
MspI CCGG 2 cut(s) 434, 524
MunI CAATTG 2 cut(s) 102, 240
NdeII GATC 4 cut(s) 36, 58, 183, 197
NlaIII CATG 2 cut(s) 254, 371
NlaIV GGNNCC 2 cut(s) 199, 215
NsiI ATGCAT 1 cut(s) 151
NspV TTCGAA 1 cut(s) 499
OliI CACNNNNGTG 1 cut(s) 524
PciSI GCTCTTC 1 cut(s) 462
PfeI GAWTC 3 cut(s) 9, 110, 353
PflMI CCANNNNNTGG 1 cut(s) 373
PinAI ACCGGT 1 cut(s) 523
PkrI GCNGC 4 cut(s) 301, 316, 319, 407
Psp124BI GAGCTC 1 cut(s) 474
PspFI CCCAGC 1 cut(s) 4
PspN4I GGNNCC 2 cut(s) 199, 215
PsuI RGATCY 2 cut(s) 183, 197
RseI CAYNNNNRTG 2 cut(s) 206, 524
SacI GAGCTC 1 cut(s) 474
SapI GCTCTTC 1 cut(s) 462
SaqAI TTAA 2 cut(s) 180, 264
SatI GCNGC 4 cut(s) 300, 315, 318, 406
Sau3AI GATC 4 cut(s) 36, 58, 183, 197
SduI GDGCHC 2 cut(s) 474, 521
SetI ASST 8 cut(s) 93, 226, 304, 322, 375, 399, 474, 481
SfaNI GCATC 3 cut(s) 158, 439, 457
SfuI TTCGAA 1 cut(s) 499
SgrAI CRCCGGYG 1 cut(s) 523
SmiMI CAYNNNNRTG 2 cut(s) 206, 524
Sse9I AATT 3 cut(s) 102, 240, 359
SsiI CCGC 2 cut(s) 211, 406
SspMI CTAG 1 cut(s) 66
SstI GAGCTC 1 cut(s) 474
TaaI ACNGT 1 cut(s) 347
TaqI TCGA 3 cut(s) 204, 465, 499
TaqII GACCGA 1 cut(s) 379
TasI AATT 3 cut(s) 102, 240, 359
TauI GCSGC 1 cut(s) 408
TfiI GAWTC 3 cut(s) 9, 110, 353
Tru1I TTAA 2 cut(s) 180, 264
Tru9I TTAA 2 cut(s) 180, 264
TseI GCWGC 3 cut(s) 299, 314, 317
TspDTI ATGAA 3 cut(s) 37, 267, 366
Van91I CCANNNNNTGG 1 cut(s) 373
XapI RAATTY 1 cut(s) 359
XspI CTAG 1 cut(s) 66
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.