Rroxscaffold_2G00145920

Mitogen-activated protein kinase kinase kinase 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
N/A
Physical Location & Seq
Forward (+)
83777560 .. 83782971
5412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145920.1

Sequence Viewer

Length: 810 bp
ATGTTTTTAGGCCGATTTGTTCGTGCTGCTCAAGGGGCCAATAAGCTTCCATTATTGCAATTGGGGTGCTTGGCGAACAACCTTGCAGAACTATCTCTTCCAAATTACAGCATGCTTTGTTATGCCCCTTACAGCAGCTTCTGCAATTTTTTGGGCCAATTACATAATGCTCCCTTCAAAGAGGCCATGGAATGCTACCTTGCAACATTAATTACACACTTTACGTACCAGTCCTCGGATCTGCGCCACTCTGTCAAGGATGTCCATTGCCTATGTTGCGATAACCAAAGTTCTAGTTTACCTACCATCAGAAAGATAGTCAACATGAGTACAAATGTGTTGTCGAGAAGCACTGCCCACCAACAATCCCTACAGAGTACCAAGCATAGCAAGTGCACTTTGTCGGGTTCCAAGAACAGGCCGAGACCTCCAATTGTGATCAACAAGGAATGGTGGGCAAAAGCTATCTCGCTCTATCAGAACACCAATTGCGTTGAGCTTCACATCCACAACTTGCTTTCCTGCATCCCAATTGTCATTAAAGCGATTGAAATTTCAGGAGAAGTGTCAGGGTCGGATTTGAGTGAGATGCAAAGGAGAAAAAATTGTGTCAGCCCTTTATTGAAATTGAAACATTACAACGATACCATGCCAAAAGACATAAAAAATAAAGTAAACGATGCAAAAAGGAAAAGATGCAAAAATTGCAGCGAGAAATACAAAAGAATTAAAAAAATGCAAGAGCAAAGAGCGTCGAAACGCAGCGCGATTTTACACTACGGATTGCAGTCGTATAGTGATTTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1221 Pkinase_Tyr

Protein Analysis

269

Amino Acids

30.59

Weight (kDa)

9.5

Isoelectric Point (pI)

60.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclin_C PF02984 2 - 110 4e-06 Cyclin, C-terminal domain
DUF1221 PF06760 148 - 205 1.1e-22 Protein of unknown function (DUF1221)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 767
AclWI GGATC 1 cut(s) 246
AcsI RAATTY 1 cut(s) 552
AfaI GTAC 3 cut(s) 227, 331, 379
AfiI CCNNNNNNNGG 2 cut(s) 235, 417
AgsI TTSAA 4 cut(s) 178, 551, 625, 631
AluBI AGCT 4 cut(s) 46, 138, 464, 499
AluI AGCT 4 cut(s) 46, 138, 464, 499
Alw21I GWGCWC 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 418
Alw44I GTGCAC 1 cut(s) 394
AlwI GGATC 1 cut(s) 246
AlwNI CAGNNNCTG 1 cut(s) 141
AoxI GGCC 5 cut(s) 10, 36, 154, 183, 419
ApaLI GTGCAC 1 cut(s) 394
ApeKI GCWGC 4 cut(s) 26, 135, 708, 762
ApoI RAATTY 1 cut(s) 552
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AseI ATTAAT 1 cut(s) 209
AspLEI GCGC 2 cut(s) 246, 767
AspS9I GGNCC 2 cut(s) 36, 154
BaeGI GKGCMC 1 cut(s) 398
Bbv12I GWGCWC 1 cut(s) 398
BbvI GCAGC 4 cut(s) 13, 147, 720, 774
BccI CCATC 1 cut(s) 314
BclI TGATCA 1 cut(s) 438
BcoDI GTCTC 1 cut(s) 418
BfaI CTAG 1 cut(s) 294
BfmI CTRYAG 1 cut(s) 371
BisI GCNGC 4 cut(s) 27, 136, 709, 763
BlsI GCNGC 4 cut(s) 28, 137, 710, 764
BmgT120I GGNCC 2 cut(s) 36, 154
BmiI GGNNCC 2 cut(s) 37, 409
BmsI GCATC 4 cut(s) 534, 579, 670, 686
BpuEI CTTGAG 1 cut(s) 15
BsaAI YACGTR 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 418
BsaJI CCNNGG 2 cut(s) 186, 234
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 417
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 265
BseDI CCNNGG 2 cut(s) 186, 234
BseGI GGATG 3 cut(s) 265, 504, 525
BseLI CCNNNNNNNGG 2 cut(s) 235, 417
BseMI GCAATG 1 cut(s) 265
BseNI ACTGG 1 cut(s) 229
BseSI GKGCMC 1 cut(s) 398
BseXI GCAGC 4 cut(s) 13, 147, 720, 774
Bsh1236I CGCG 1 cut(s) 767
BshFI GGCC 5 cut(s) 12, 38, 156, 185, 421
BsiHKAI GWGCWC 1 cut(s) 398
BslI CCNNNNNNNGG 2 cut(s) 235, 417
BsmAI GTCTC 1 cut(s) 418
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 5 cut(s) 12, 38, 156, 185, 421
Bso31I GGTCTC 1 cut(s) 418
Bsp1286I GDGCHC 1 cut(s) 398
Bsp143I GATC 2 cut(s) 238, 438
Bsp19I CCATGG 1 cut(s) 186
BspANI GGCC 5 cut(s) 12, 38, 156, 185, 421
BspFNI CGCG 1 cut(s) 767
BspLI GGNNCC 2 cut(s) 37, 409
BspPI GGATC 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 418
BsrDI GCAATG 1 cut(s) 265
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 2 cut(s) 186, 234
BssMI GATC 2 cut(s) 238, 438
BssT1I CCWWGG 1 cut(s) 186
Bst6I CTCTTC 1 cut(s) 102
BstAPI GCANNNNNTGC 2 cut(s) 141, 705
BstBAI YACGTR 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 113
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 3 cut(s) 265, 504, 525
BstFNI CGCG 1 cut(s) 767
BstHHI GCGC 2 cut(s) 246, 767
BstKTI GATC 2 cut(s) 241, 441
BstMAI GTCTC 1 cut(s) 418
BstMBI GATC 2 cut(s) 238, 438
BstMWI GCNNNNNNNGC 4 cut(s) 35, 141, 276, 705
BstNSI RCATGY 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 371
BstSLI GKGCMC 1 cut(s) 398
BstSNI TACGTA 1 cut(s) 225
BstUI CGCG 1 cut(s) 767
BstV1I GCAGC 4 cut(s) 13, 147, 720, 774
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 5 cut(s) 12, 38, 156, 185, 421
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 3 cut(s) 265, 504, 525
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 1 cut(s) 351
Cac8I GCNNGC 1 cut(s) 113
CaiI CAGNNNCTG 1 cut(s) 141
CfoI GCGC 2 cut(s) 246, 767
Cfr13I GGNCC 2 cut(s) 36, 154
CseI GACGC 1 cut(s) 741
Csp6I GTAC 3 cut(s) 226, 330, 378
CviAII CATG 4 cut(s) 112, 187, 325, 649
CviQI GTAC 3 cut(s) 226, 330, 378
DpnI GATC 2 cut(s) 240, 440
DpnII GATC 2 cut(s) 238, 438
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Eco105I TACGTA 1 cut(s) 225
Eco130I CCWWGG 1 cut(s) 186
Eco31I GGTCTC 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 4 cut(s) 115, 190, 328, 652
FatI CATG 4 cut(s) 111, 186, 324, 648
FbaI TGATCA 1 cut(s) 438
Fnu4HI GCNGC 4 cut(s) 27, 136, 709, 763
FokI GGATG 3 cut(s) 272, 491, 512
Fsp4HI GCNGC 4 cut(s) 27, 136, 709, 763
FspBI CTAG 1 cut(s) 294
GlaI GCGC 2 cut(s) 245, 766
GluI GCNGC 4 cut(s) 27, 136, 709, 763
HaeIII GGCC 5 cut(s) 12, 38, 156, 185, 421
HgaI GACGC 1 cut(s) 741
HhaI GCGC 2 cut(s) 246, 767
Hin1II CATG 4 cut(s) 115, 190, 328, 652
Hin6I GCGC 2 cut(s) 244, 765
HinP1I GCGC 2 cut(s) 244, 765
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 44
Hpy166II GTNNAC 4 cut(s) 299, 322, 396, 676
Hpy188I TCNGA 4 cut(s) 238, 311, 480, 577
Hpy188III TCNNGA 2 cut(s) 345, 558
Hpy8I GTNNAC 4 cut(s) 299, 322, 396, 676
Hpy99I CGWCG 1 cut(s) 757
HpyAV CCTTC 1 cut(s) 184
HpyCH4IV ACGT 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 141, 276, 705
HpySE526I ACGT 1 cut(s) 224
Hsp92II CATG 4 cut(s) 115, 190, 328, 652
HspAI GCGC 2 cut(s) 244, 765
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 2 cut(s) 238, 438
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 5 cut(s) 242, 403, 535, 543, 555
Lsp1109I GCAGC 4 cut(s) 13, 147, 720, 774
LweI GCATC 4 cut(s) 534, 579, 670, 686
MaeI CTAG 1 cut(s) 294
MaeII ACGT 1 cut(s) 224
MalI GATC 2 cut(s) 240, 440
MboI GATC 2 cut(s) 238, 438
MboII GAAGA 1 cut(s) 89
MfeI CAATTG 4 cut(s) 59, 432, 487, 531
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 398
MmeI TCCRAC 1 cut(s) 555
MnlI CCTC 3 cut(s) 175, 244, 438
MseI TTAA 3 cut(s) 209, 540, 729
MunI CAATTG 4 cut(s) 59, 432, 487, 531
Mva1269I GAATGC 1 cut(s) 197
MvnI CGCG 1 cut(s) 767
MwoI GCNNNNNNNGC 4 cut(s) 35, 141, 276, 705
NcoI CCATGG 1 cut(s) 186
NdeII GATC 2 cut(s) 238, 438
NlaIII CATG 4 cut(s) 115, 190, 328, 652
NlaIV GGNNCC 2 cut(s) 37, 409
NmeAIII GCCGAG 1 cut(s) 447
NspI RCATGY 1 cut(s) 115
PaeI GCATGC 1 cut(s) 115
PctI GAATGC 1 cut(s) 197
PkrI GCNGC 4 cut(s) 28, 137, 710, 764
Ppu21I YACGTR 1 cut(s) 225
PshBI ATTAAT 1 cut(s) 209
PspN4I GGNNCC 2 cut(s) 37, 409
PspPI GGNCC 2 cut(s) 36, 154
PstNI CAGNNNCTG 1 cut(s) 141
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 3 cut(s) 227, 331, 379
RsaNI GTAC 3 cut(s) 226, 330, 378
SaqAI TTAA 3 cut(s) 209, 540, 729
SatI GCNGC 4 cut(s) 27, 136, 709, 763
Sau3AI GATC 2 cut(s) 238, 438
Sau96I GGNCC 2 cut(s) 36, 154
SduI GDGCHC 1 cut(s) 398
SetI ASST 9 cut(s) 48, 84, 140, 201, 227, 304, 430, 466, 501
SfaNI GCATC 4 cut(s) 534, 579, 670, 686
SfcI CTRYAG 1 cut(s) 371
SmlI CTYRAG 1 cut(s) 30
SmoI CTYRAG 1 cut(s) 30
SnaBI TACGTA 1 cut(s) 225
SphI GCATGC 1 cut(s) 115
SspMI CTAG 1 cut(s) 294
StyI CCWWGG 1 cut(s) 186
TaiI ACGT 1 cut(s) 227
TaqI TCGA 2 cut(s) 344, 755
TatI WGTACW 1 cut(s) 329
Tru1I TTAA 3 cut(s) 209, 540, 729
Tru9I TTAA 3 cut(s) 209, 540, 729
TscAI CASTG 1 cut(s) 358
TseI GCWGC 4 cut(s) 26, 135, 708, 762
TspGWI ACGGA 1 cut(s) 795
TspRI CASTG 1 cut(s) 358
VneI GTGCAC 1 cut(s) 394
VspI ATTAAT 1 cut(s) 209
XapI RAATTY 1 cut(s) 552
XceI RCATGY 1 cut(s) 115
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.