Rroxscaffold_3G00218150

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
N/A
Physical Location & Seq
Reverse (-)
179352 .. 193624
14273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00218150.1

Sequence Viewer

Length: 477 bp
ATGCGCGGACAAACGTTGCGGAATTTTGACATCCATAAGAATTTTTCACCATCCCACGCGTCGCCCACGGTGGGCGAATCAAGATTTGCTCTCATTGTTCTCTCACAAAATTATGCATCGTCAACATGGTGCTTGGATGAACTCGTAAGAATTATTAAATGTATGAAAGCAAGAGAAACAGTGCTGCCAATTTTCTATGATGTTGATCCCTCCGATGTACGAAAGCAAACAAGCGACTTTAGAGAAGCCTTCGCTACTCATGAAGAAAGGTTTAGGGATGACAAAGAGAAAGACACTATTCTGCCCTACGAGACTTGTATTAGACGGCCACTACAGTGTCCCAAAAGCTTAGACTGTCAAGGAATAACCAACAATATATCTGTAGTTAGCACCGTTATTCACGGAGTCACGACCAATCCCCTAAACCTGCATATACCTAATTTGAAACCAGAACCTCTTGCGCAAACAAGGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

158

Amino Acids

18.06

Weight (kDa)

7.68

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 94 6.3e-22 TIR domain
TIR_2 PF13676 24 - 88 1.2e-06 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 462
Acc36I ACCTGC 1 cut(s) 435
AccII CGCG 2 cut(s) 6, 59
AciI CCGC 2 cut(s) 6, 19
AclI AACGTT 1 cut(s) 14
AclWI GGATC 1 cut(s) 200
AcoI YGGCCR 1 cut(s) 326
AcsI RAATTY 2 cut(s) 22, 40
AfaI GTAC 1 cut(s) 219
AfiI CCNNNNNNNGG 1 cut(s) 71
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 1 cut(s) 445
AleI CACNNNNGTG 1 cut(s) 334
AluBI AGCT 1 cut(s) 348
AluI AGCT 1 cut(s) 348
Alw26I GTCTC 1 cut(s) 305
AlwI GGATC 1 cut(s) 200
AoxI GGCC 1 cut(s) 326
ApeKI GCWGC 1 cut(s) 184
ApoI RAATTY 2 cut(s) 22, 40
AspLEI GCGC 2 cut(s) 6, 463
AsuHPI GGTGA 1 cut(s) 39
BbvI GCAGC 1 cut(s) 171
BccI CCATC 1 cut(s) 58
BceAI ACGGC 1 cut(s) 341
BcoDI GTCTC 1 cut(s) 305
BfmI CTRYAG 2 cut(s) 332, 381
BfuAI ACCTGC 1 cut(s) 435
BisI GCNGC 1 cut(s) 185
BlsI GCNGC 1 cut(s) 186
BmsI GCATC 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 66
Bsc4I CCNNNNNNNGG 1 cut(s) 71
Bse8I GATNNNNATC 1 cut(s) 204
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 4 cut(s) 30, 50, 142, 283
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 171
Bsh1236I CGCG 2 cut(s) 6, 59
BshFI GGCC 1 cut(s) 328
BslFI GGGAC 1 cut(s) 324
BslI CCNNNNNNNGG 1 cut(s) 71
BsmAI GTCTC 1 cut(s) 305
BsmFI GGGAC 1 cut(s) 324
BsnI GGCC 1 cut(s) 328
Bsp143I GATC 1 cut(s) 205
BspACI CCGC 2 cut(s) 6, 19
BspANI GGCC 1 cut(s) 328
BspFNI CGCG 2 cut(s) 6, 59
BspHI TCATGA 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 435
BspPI GGATC 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 1 cut(s) 205
Bst4CI ACNGT 5 cut(s) 70, 181, 336, 356, 394
BstDEI CTNAG 1 cut(s) 349
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 4 cut(s) 30, 50, 142, 283
BstFNI CGCG 2 cut(s) 6, 59
BstHHI GCGC 2 cut(s) 6, 463
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 1 cut(s) 469
BstSFI CTRYAG 2 cut(s) 332, 381
BstUI CGCG 2 cut(s) 6, 59
BstV1I GCAGC 1 cut(s) 171
BsuRI GGCC 1 cut(s) 328
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 4 cut(s) 30, 50, 142, 283
BtsIMutI CAGTG 2 cut(s) 186, 341
BveI ACCTGC 1 cut(s) 435
CciI TCATGA 1 cut(s) 259
CfoI GCGC 2 cut(s) 6, 463
CseI GACGC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 218
CviAII CATG 2 cut(s) 126, 260
CviJI RGCY 4 cut(s) 248, 328, 348, 472
CviKI_1 RGCY 4 cut(s) 248, 328, 348, 472
CviQI GTAC 1 cut(s) 218
DdeI CTNAG 1 cut(s) 349
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 326
EcoT22I ATGCAT 1 cut(s) 118
FaeI CATG 2 cut(s) 129, 263
FaiI YATR 9 cut(s) 36, 114, 127, 164, 198, 261, 377, 432, 434
FaqI GGGAC 1 cut(s) 324
FatI CATG 2 cut(s) 125, 259
Fnu4HI GCNGC 1 cut(s) 185
FokI GGATG 4 cut(s) 17, 37, 149, 290
Fsp4HI GCNGC 1 cut(s) 185
FspI TGCGCA 1 cut(s) 462
GlaI GCGC 2 cut(s) 5, 462
GluI GCNGC 1 cut(s) 185
HaeIII GGCC 1 cut(s) 328
HgaI GACGC 1 cut(s) 48
HhaI GCGC 2 cut(s) 6, 463
Hin1II CATG 2 cut(s) 129, 263
Hin6I GCGC 2 cut(s) 4, 461
HinP1I GCGC 2 cut(s) 4, 461
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 346
HinfI GANTC 2 cut(s) 77, 405
HphI GGTGA 1 cut(s) 39
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 2 cut(s) 214, 476
Hpy188III TCNNGA 3 cut(s) 81, 260, 409
Hpy8I GTNNAC 1 cut(s) 123
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 5 cut(s) 70, 181, 336, 356, 394
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 2 cut(s) 116, 430
HpyF10VI GCNNNNNNNGC 1 cut(s) 469
HpyF3I CTNAG 1 cut(s) 349
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 2 cut(s) 129, 263
HspAI GCGC 2 cut(s) 4, 461
Kzo9I GATC 1 cut(s) 205
LpnPI CCDG 2 cut(s) 440, 462
Lsp1109I GCAGC 1 cut(s) 171
LweI GCATC 1 cut(s) 125
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 1 cut(s) 406
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 1 cut(s) 275
MluCI AATT 6 cut(s) 22, 40, 109, 150, 189, 439
MluI ACGCGT 1 cut(s) 57
MlyI GAGTC 1 cut(s) 414
MnlI CCTC 2 cut(s) 220, 465
Mph1103I ATGCAT 1 cut(s) 118
MseI TTAA 1 cut(s) 156
MslI CAYNNNNRTG 1 cut(s) 334
MvnI CGCG 2 cut(s) 6, 59
MwoI GCNNNNNNNGC 1 cut(s) 469
NdeII GATC 1 cut(s) 205
NlaIII CATG 2 cut(s) 129, 263
NmuCI GTSAC 1 cut(s) 406
NsbI TGCGCA 1 cut(s) 462
NsiI ATGCAT 1 cut(s) 118
OliI CACNNNNGTG 1 cut(s) 334
PagI TCATGA 1 cut(s) 259
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 1 cut(s) 186
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
Psp1406I AACGTT 1 cut(s) 14
RsaI GTAC 1 cut(s) 219
RsaNI GTAC 1 cut(s) 218
RseI CAYNNNNRTG 1 cut(s) 334
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 1 cut(s) 185
Sau3AI GATC 1 cut(s) 205
SchI GAGTC 1 cut(s) 414
SetI ASST 6 cut(s) 17, 272, 350, 429, 439, 457
SfaNI GCATC 1 cut(s) 125
SfcI CTRYAG 2 cut(s) 332, 381
SmiMI CAYNNNNRTG 1 cut(s) 334
Sse9I AATT 6 cut(s) 22, 40, 109, 150, 189, 439
SsiI CCGC 2 cut(s) 6, 19
TaaI ACNGT 5 cut(s) 70, 181, 336, 356, 394
TaiI ACGT 1 cut(s) 17
TasI AATT 6 cut(s) 22, 40, 109, 150, 189, 439
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 2 cut(s) 186, 341
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 1 cut(s) 184
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 3 cut(s) 153, 179, 276
TspGWI ACGGA 1 cut(s) 417
TspRI CASTG 2 cut(s) 186, 341
XapI RAATTY 2 cut(s) 22, 40
Zsp2I ATGCAT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.