Rroxscaffold_3G00227980

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
N/A
Physical Location & Seq
Reverse (-)
12300116 .. 12301527
1412 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00227980.1

Sequence Viewer

Length: 471 bp
ATGGGAGTTCAGGTACTGTTTACTGGAAGAGCAGCCAAAGCTGTGTTCACCCGGGGTTGGAATCGGTTTGTAAATGAGAGCCTATTGAATGCCAATGACACTATCAGATTCTATGAGTGCCACATTAGGGAAGGAGATCAGGAGGCCAAGTCATTCTACATGATTAAAATCGACCGGGCTGAAAGTTCTGATGTCATTGTTGAAAATCCTAGCCAATGTGATCCCCCCATCATTCACCCCACCCAACCCCCACCCCCCATTACCTCCTTGCATGCCAAGAAGTTTGGAGAGGAGAAAGATAACATACAGACACAAATTGCAGAAGTTATTGATGAAATCCCACTAGTTAATGGGGATAATAAAAAAAAATTAGGCTCTTCGGTGTTTGTATCGTTGGAGTTTGAAGTTTATGACAATAATTTTCGCGGACTTATGACTGATTTTGAGCTAGTACAGCATGTCTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

156

Amino Acids

17.72

Weight (kDa)

5.07

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 426
AciI CCGC 1 cut(s) 426
AclWI GGATC 1 cut(s) 215
AfaI GTAC 2 cut(s) 15, 453
AfiI CCNNNNNNNGG 2 cut(s) 57, 127
AgsI TTSAA 3 cut(s) 88, 203, 404
AhlI ACTAGT 1 cut(s) 343
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 2 cut(s) 41, 448
AluI AGCT 2 cut(s) 41, 448
AlwI GGATC 1 cut(s) 215
AlwNI CAGNNNCTG 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 51
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 1 cut(s) 32
AsuC2I CCSGG 3 cut(s) 52, 53, 176
AsuHPI GGTGA 2 cut(s) 40, 227
AvaI CYCGRG 1 cut(s) 51
BbvI GCAGC 1 cut(s) 44
BccI CCATC 1 cut(s) 236
BcnI CCSGG 3 cut(s) 52, 53, 176
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 3 cut(s) 210, 344, 449
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
Bme1390I CCNGG 3 cut(s) 52, 53, 176
BmeT110I CYCGRG 1 cut(s) 51
BmrFI CCNGG 3 cut(s) 52, 53, 176
BpuMI CCSGG 3 cut(s) 52, 53, 176
BsaBI GATNNNNATC 1 cut(s) 167
BsaJI CCNNGG 2 cut(s) 51, 52
BsaXI ACNNNNNCTCC 3 cut(s) 27, 134, 164
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 127
Bse1I ACTGG 1 cut(s) 28
Bse8I GATNNNNATC 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 51, 52
BseJI GATNNNNATC 1 cut(s) 167
BseLI CCNNNNNNNGG 2 cut(s) 57, 127
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 1 cut(s) 44
Bsh1236I CGCG 1 cut(s) 426
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 175
BsiHKCI CYCGRG 1 cut(s) 51
BsiSI CCGG 2 cut(s) 52, 175
BslI CCNNNNNNNGG 2 cut(s) 57, 127
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 51
Bsp143I GATC 2 cut(s) 136, 220
BspACI CCGC 1 cut(s) 426
BspANI GGCC 1 cut(s) 146
BspFNI CGCG 1 cut(s) 426
BspPI GGATC 1 cut(s) 215
BspQI GCTCTTC 2 cut(s) 22, 382
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 2 cut(s) 51, 52
BssMI GATC 2 cut(s) 136, 220
Bst4CI ACNGT 1 cut(s) 18
Bst6I CTCTTC 2 cut(s) 22, 382
BstC8I GCNNGC 1 cut(s) 273
BstFNI CGCG 1 cut(s) 426
BstKTI GATC 2 cut(s) 139, 223
BstMBI GATC 2 cut(s) 136, 220
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 2 cut(s) 38, 454
BstNSI RCATGY 3 cut(s) 275, 461, 469
BstSCI CCNGG 3 cut(s) 50, 51, 174
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 44
BsuRI GGCC 1 cut(s) 146
Cac8I GCNNGC 1 cut(s) 273
CaiI CAGNNNCTG 1 cut(s) 16
Cfr9I CCCGGG 1 cut(s) 51
Csp6I GTAC 2 cut(s) 14, 452
CviAII CATG 4 cut(s) 160, 272, 458, 466
CviJI RGCY 8 cut(s) 35, 41, 81, 146, 179, 213, 375, 448
CviKI_1 RGCY 8 cut(s) 35, 41, 81, 146, 179, 213, 375, 448
CviQI GTAC 2 cut(s) 14, 452
DpnI GATC 2 cut(s) 138, 222
DpnII GATC 2 cut(s) 136, 220
Eam1104I CTCTTC 2 cut(s) 22, 382
EarI CTCTTC 2 cut(s) 22, 382
Eco88I CYCGRG 1 cut(s) 51
FaeI CATG 4 cut(s) 163, 275, 461, 469
FaiI YATR 8 cut(s) 114, 161, 273, 305, 411, 434, 459, 467
FatI CATG 4 cut(s) 159, 271, 457, 465
Fnu4HI GCNGC 1 cut(s) 33
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 3 cut(s) 210, 344, 449
GluI GCNGC 1 cut(s) 33
HaeIII GGCC 1 cut(s) 146
HapII CCGG 2 cut(s) 52, 175
Hin1II CATG 4 cut(s) 163, 275, 461, 469
HinfI GANTC 2 cut(s) 61, 108
HpaII CCGG 2 cut(s) 52, 175
HphI GGTGA 2 cut(s) 40, 227
Hpy166II GTNNAC 2 cut(s) 21, 48
Hpy188I TCNGA 2 cut(s) 107, 190
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 2 cut(s) 21, 48
HpyAV CCTTC 1 cut(s) 125
HpyCH4III ACNGT 1 cut(s) 18
HpyCH4V TGCA 3 cut(s) 271, 320, 465
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 454
Hsp92II CATG 4 cut(s) 163, 275, 461, 469
Kzo9I GATC 2 cut(s) 136, 220
LguI GCTCTTC 2 cut(s) 22, 382
LpnPI CCDG 4 cut(s) 9, 65, 125, 188
Lsp1109I GCAGC 1 cut(s) 44
MaeI CTAG 3 cut(s) 210, 344, 449
MalI GATC 2 cut(s) 138, 222
MboI GATC 2 cut(s) 136, 220
MboII GAAGA 2 cut(s) 39, 369
MluCI AATT 3 cut(s) 315, 368, 418
MmeI TCCRAC 2 cut(s) 38, 375
MnlI CCTC 3 cut(s) 136, 274, 283
MseI TTAA 2 cut(s) 165, 348
MspI CCGG 2 cut(s) 52, 175
MspR9I CCNGG 3 cut(s) 52, 53, 176
Mva1269I GAATGC 1 cut(s) 94
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 2 cut(s) 38, 454
NciI CCSGG 3 cut(s) 52, 53, 176
NdeII GATC 2 cut(s) 136, 220
NlaIII CATG 4 cut(s) 163, 275, 461, 469
NspI RCATGY 3 cut(s) 275, 461, 469
PaeI GCATGC 1 cut(s) 275
PciSI GCTCTTC 2 cut(s) 22, 382
PctI GAATGC 1 cut(s) 94
PfeI GAWTC 2 cut(s) 61, 108
PkrI GCNGC 1 cut(s) 34
PstNI CAGNNNCTG 1 cut(s) 16
RsaI GTAC 2 cut(s) 15, 453
RsaNI GTAC 2 cut(s) 14, 452
SapI GCTCTTC 2 cut(s) 22, 382
SaqAI TTAA 2 cut(s) 165, 348
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 2 cut(s) 136, 220
ScrFI CCNGG 3 cut(s) 52, 53, 176
SetI ASST 4 cut(s) 15, 43, 266, 450
SmaI CCCGGG 1 cut(s) 53
SpeI ACTAGT 1 cut(s) 343
SphI GCATGC 1 cut(s) 275
Sse9I AATT 3 cut(s) 315, 368, 418
SsiI CCGC 1 cut(s) 426
SspMI CTAG 3 cut(s) 210, 344, 449
StyD4I CCNGG 3 cut(s) 50, 51, 174
TaaI ACNGT 1 cut(s) 18
TaqI TCGA 1 cut(s) 171
TasI AATT 3 cut(s) 315, 368, 418
TatI WGTACW 1 cut(s) 451
TfiI GAWTC 2 cut(s) 61, 108
Tru1I TTAA 2 cut(s) 165, 348
Tru9I TTAA 2 cut(s) 165, 348
TseI GCWGC 1 cut(s) 32
TspDTI ATGAA 1 cut(s) 348
TspMI CCCGGG 1 cut(s) 51
XceI RCATGY 3 cut(s) 275, 461, 469
XmaI CCCGGG 1 cut(s) 51
XspI CTAG 3 cut(s) 210, 344, 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.