Rroxscaffold_3G00243640

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
N/A
Physical Location & Seq
Forward (+)
36101946 .. 36102655
710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00243640.1

Sequence Viewer

Length: 564 bp
ATGGGAAGTAGAATCATCATAGCAACAAGGGATAAGCAATTGCCGACCAGTCATAGTATTAATTTAATATACAAGGTCCAAGAATTGGGGCATCATGAAGCTCTCGAGCTTTTTAGTCGGATTGTATTTAAAAACGGACCTTTAGATGGTTTTGCGGTACTCACGAACGTGCAATATGCTATGCTCAAGGAAATTTTTCTTGACATTGCATGTTTCTTCAAAGGTGAATCTACAAACCATGTGATACAAACGCTAGAAGCTTATCGTGGCAAACCTGCTAAGTATGGTATCAAAGTGCTCGTAGACAAGGCCCACATCAATACATCTAGGGGTTGCATTTGGATGCAAGACTTGCTAGAAGAAATGAGTAGAGATAGTCCATCAACGATCCATTTGGATAACCACGAAAAACGTAGCAGGTTGTGGTTTCATGAAAATGTTTACCATGTTCTAACGAGAGTTAAAGATGTCTTGAAGCGACAACATACTCCGGAAAACTATGGGTGTTCATGTTCATACTCCAAACTATGGATTTGTTTGACCAAAAAACGGATGTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

187

Amino Acids

21.66

Weight (kDa)

9.19

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 63 - 125 6e-11 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 283, 408
AccB7I CCANNNNNTGG 2 cut(s) 85, 528
AccI GTMKAC 1 cut(s) 303
AccIII TCCGGA 1 cut(s) 490
AciI CCGC 1 cut(s) 155
AclWI GGATC 1 cut(s) 382
AcsI RAATTY 1 cut(s) 192
AfaI GTAC 1 cut(s) 159
AfiI CCNNNNNNNGG 4 cut(s) 85, 146, 528, 549
AgsI TTSAA 2 cut(s) 220, 475
AleI CACNNNNGTG 1 cut(s) 167
AluBI AGCT 3 cut(s) 101, 109, 260
AluI AGCT 3 cut(s) 101, 109, 260
Alw21I GWGCWC 1 cut(s) 300
AlwI GGATC 1 cut(s) 382
Ama87I CYCGRG 1 cut(s) 104
Aor13HI TCCGGA 1 cut(s) 490
AoxI GGCC 1 cut(s) 309
ApoI RAATTY 1 cut(s) 192
AseI ATTAAT 1 cut(s) 60
Asp700I GAANNNNTTC 1 cut(s) 195
AspS9I GGNCC 3 cut(s) 76, 137, 310
AsuHPI GGTGA 1 cut(s) 236
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 2 cut(s) 76, 137
Bbv12I GWGCWC 1 cut(s) 300
BccI CCATC 2 cut(s) 140, 388
BfaI CTAG 3 cut(s) 254, 327, 356
BfuAI ACCTGC 2 cut(s) 283, 408
Bme18I GGWCC 2 cut(s) 76, 137
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 3 cut(s) 76, 137, 310
BmsI GCATC 2 cut(s) 100, 333
BpuEI CTTGAG 1 cut(s) 170
BsaWI WCCGGW 1 cut(s) 490
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 146, 528, 549
Bse1I ACTGG 1 cut(s) 48
Bse3DI GCAATG 1 cut(s) 204
BseAI TCCGGA 1 cut(s) 490
BseGI GGATG 2 cut(s) 348, 558
BseLI CCNNNNNNNGG 4 cut(s) 85, 146, 528, 549
BseMI GCAATG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 48
BshFI GGCC 1 cut(s) 311
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 491
BslI CCNNNNNNNGG 4 cut(s) 85, 146, 528, 549
BsnI GGCC 1 cut(s) 311
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 300
Bsp13I TCCGGA 1 cut(s) 490
Bsp143I GATC 1 cut(s) 387
BspACI CCGC 1 cut(s) 155
BspANI GGCC 1 cut(s) 311
BspEI TCCGGA 1 cut(s) 490
BspHI TCATGA 2 cut(s) 94, 430
BspMI ACCTGC 2 cut(s) 283, 408
BspPI GGATC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 204
BsrI ACTGG 1 cut(s) 48
BssMI GATC 1 cut(s) 387
BstAPI GCANNNNNTGC 1 cut(s) 352
BstDEI CTNAG 1 cut(s) 279
BstF5I GGATG 2 cut(s) 348, 558
BstKTI GATC 1 cut(s) 390
BstMBI GATC 1 cut(s) 387
BstMWI GCNNNNNNNGC 1 cut(s) 352
BstNSI RCATGY 1 cut(s) 213
BsuRI GGCC 1 cut(s) 311
BtsCI GGATG 2 cut(s) 348, 558
BveI ACCTGC 2 cut(s) 283, 408
CciI TCATGA 2 cut(s) 94, 430
Cfr13I GGNCC 3 cut(s) 76, 137, 310
Csp6I GTAC 1 cut(s) 158
CviAII CATG 6 cut(s) 95, 210, 239, 431, 446, 510
CviJI RGCY 4 cut(s) 101, 109, 260, 311
CviKI_1 RGCY 4 cut(s) 101, 109, 260, 311
CviQI GTAC 1 cut(s) 158
DdeI CTNAG 1 cut(s) 279
DpnI GATC 1 cut(s) 389
DpnII GATC 1 cut(s) 387
DraI TTTAAA 1 cut(s) 130
Eco47I GGWCC 2 cut(s) 76, 137
Eco88I CYCGRG 1 cut(s) 104
FaeI CATG 6 cut(s) 98, 213, 242, 434, 449, 513
FatI CATG 6 cut(s) 94, 209, 238, 430, 445, 509
FblI GTMKAC 1 cut(s) 303
FokI GGATG 1 cut(s) 355
FspBI CTAG 3 cut(s) 254, 327, 356
HaeIII GGCC 1 cut(s) 311
HapII CCGG 1 cut(s) 491
Hin1II CATG 6 cut(s) 98, 213, 242, 434, 449, 513
HindIII AAGCTT 1 cut(s) 258
HinfI GANTC 2 cut(s) 12, 227
HpaII CCGG 1 cut(s) 491
HphI GGTGA 1 cut(s) 236
Hpy166II GTNNAC 2 cut(s) 304, 442
Hpy188I TCNGA 1 cut(s) 120
Hpy188III TCNNGA 7 cut(s) 95, 104, 163, 200, 431, 472, 491
Hpy8I GTNNAC 2 cut(s) 304, 442
HpyCH4IV ACGT 2 cut(s) 168, 412
HpyCH4V TGCA 4 cut(s) 172, 209, 336, 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 352
HpyF3I CTNAG 1 cut(s) 279
HpySE526I ACGT 2 cut(s) 168, 412
Hsp92II CATG 6 cut(s) 98, 213, 242, 434, 449, 513
Kpn2I TCCGGA 1 cut(s) 490
Kzo9I GATC 1 cut(s) 387
LpnPI CCDG 4 cut(s) 61, 288, 403, 504
LweI GCATC 2 cut(s) 100, 333
MaeI CTAG 3 cut(s) 254, 327, 356
MaeII ACGT 2 cut(s) 168, 412
MalI GATC 1 cut(s) 389
MboI GATC 1 cut(s) 387
MboII GAAGA 2 cut(s) 208, 371
MfeI CAATTG 1 cut(s) 38
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 4 cut(s) 38, 61, 83, 192
MmeI TCCRAC 1 cut(s) 98
MroI TCCGGA 1 cut(s) 490
MroXI GAANNNNTTC 1 cut(s) 195
MseI TTAA 5 cut(s) 60, 65, 129, 462, 562
MslI CAYNNNNRTG 3 cut(s) 167, 341, 435
MspI CCGG 1 cut(s) 491
MunI CAATTG 1 cut(s) 38
MwoI GCNNNNNNNGC 1 cut(s) 352
NdeII GATC 1 cut(s) 387
NlaIII CATG 6 cut(s) 98, 213, 242, 434, 449, 513
NspI RCATGY 1 cut(s) 213
OliI CACNNNNGTG 1 cut(s) 167
PaeR7I CTCGAG 1 cut(s) 104
PagI TCATGA 2 cut(s) 94, 430
PdmI GAANNNNTTC 1 cut(s) 195
PfeI GAWTC 2 cut(s) 12, 227
PflMI CCANNNNNTGG 2 cut(s) 85, 528
PshBI ATTAAT 1 cut(s) 60
PspPI GGNCC 3 cut(s) 76, 137, 310
RsaI GTAC 1 cut(s) 159
RsaNI GTAC 1 cut(s) 158
RseI CAYNNNNRTG 3 cut(s) 167, 341, 435
SaqAI TTAA 5 cut(s) 60, 65, 129, 462, 562
Sau3AI GATC 1 cut(s) 387
Sau96I GGNCC 3 cut(s) 76, 137, 310
SduI GDGCHC 1 cut(s) 300
SfaNI GCATC 2 cut(s) 100, 333
Sfr274I CTCGAG 1 cut(s) 104
SinI GGWCC 2 cut(s) 76, 137
SlaI CTCGAG 1 cut(s) 104
SmiMI CAYNNNNRTG 3 cut(s) 167, 341, 435
SmlI CTYRAG 2 cut(s) 104, 185
SmoI CTYRAG 2 cut(s) 104, 185
Sse9I AATT 4 cut(s) 38, 61, 83, 192
SsiI CCGC 1 cut(s) 155
SspMI CTAG 3 cut(s) 254, 327, 356
TaiI ACGT 2 cut(s) 171, 415
TaqI TCGA 1 cut(s) 105
TasI AATT 4 cut(s) 38, 61, 83, 192
TfiI GAWTC 2 cut(s) 12, 227
Tru1I TTAA 5 cut(s) 60, 65, 129, 462, 562
Tru9I TTAA 5 cut(s) 60, 65, 129, 462, 562
TspDTI ATGAA 5 cut(s) 111, 419, 447, 498, 504
TspGWI ACGGA 1 cut(s) 150
Van91I CCANNNNNTGG 2 cut(s) 85, 528
VpaK11BI GGWCC 2 cut(s) 76, 137
VspI ATTAAT 1 cut(s) 60
XapI RAATTY 1 cut(s) 192
XceI RCATGY 1 cut(s) 213
XhoI CTCGAG 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 303
XmnI GAANNNNTTC 1 cut(s) 195
XspI CTAG 3 cut(s) 254, 327, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.