Rroxscaffold_3G00251370
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
N/A
Physical Location & Seq
Reverse (-)
45278553 .. 45285910
7358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00251370.1

Sequence Viewer

Length: 453 bp
ATGGAGAAAGAGGGATCCAACAGCTATTGGTATGTGAATATATGCCTAATAGTAGCTAGCTTAGAAGATCATCTTTGCAACAGGGTTTTGAACCCTCTTCCTTGGATCACGAGGTTACAAATAATGCTTGGTGCTGCTCAAGCTAAGATTGGCTTATCTACACAAGGGACAGGAAGTCCAGTCTGTTCTCTAAAAGCTTTAGCTGTTGGGACCCTGTGGTTGTGTCACATGGTGATATATCGAGATTTCAAATCCTCCAACGTGCTCTTGGATGAGGACTTTAAGCCAAAGCACTCAGACTTTGGGCTTGCTAGAGAAGGGCCAAAGGGTGACCATACTCATGTATCGACAGCAGTGGCAGGGACCTATGGATATGCTGCCCCAGAGAATGTTGAAACAGGCCATCTTTCCATCCATAGTGACTTAGGGAGTTTTTGGTGTGGTGCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

150

Amino Acids

16.33

Weight (kDa)

6.13

Isoelectric Point (pI)

19.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 67 - 145 8.2e-09 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 75 - 142 1.2e-12 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 9, 22, 113
AdeI CACNNNGTG 1 cut(s) 232
AgsI TTSAA 3 cut(s) 91, 250, 395
AhdI GACNNNNNGTC 1 cut(s) 174
AluBI AGCT 6 cut(s) 24, 56, 60, 143, 197, 203
AluI AGCT 6 cut(s) 24, 56, 60, 143, 197, 203
Alw21I GWGCWC 1 cut(s) 267
AlwI GGATC 3 cut(s) 9, 22, 113
AoxI GGCC 2 cut(s) 320, 400
ApeKI GCWGC 2 cut(s) 134, 377
AspS9I GGNCC 3 cut(s) 210, 320, 363
AsuHPI GGTGA 2 cut(s) 244, 341
AsuNHI GCTAGC 1 cut(s) 56
AvaII GGWCC 2 cut(s) 210, 363
BaeI ACNNNNGTAYC 2 cut(s) 327, 360
BamHI GGATCC 1 cut(s) 14
BauI CACGAG 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 2 cut(s) 121, 364
BccI CCATC 2 cut(s) 411, 419
BfaI CTAG 2 cut(s) 57, 312
BisI GCNGC 2 cut(s) 135, 378
BlsI GCNGC 2 cut(s) 136, 379
Bme18I GGWCC 2 cut(s) 210, 363
BmeRI GACNNNNNGTC 1 cut(s) 174
BmgT120I GGNCC 3 cut(s) 210, 320, 363
BmiI GGNNCC 4 cut(s) 16, 211, 212, 364
BmtI GCTAGC 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 179
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 2 cut(s) 277, 411
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 1 cut(s) 179
BseXI GCAGC 2 cut(s) 121, 364
BshFI GGCC 2 cut(s) 322, 402
BsiHKAI GWGCWC 1 cut(s) 267
BslFI GGGAC 3 cut(s) 181, 223, 376
BsmFI GGGAC 3 cut(s) 181, 223, 376
BsnI GGCC 2 cut(s) 322, 402
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 3 cut(s) 14, 67, 105
BspANI GGCC 2 cut(s) 322, 402
BspCNI CTCAG 1 cut(s) 308
BspLI GGNNCC 4 cut(s) 16, 211, 212, 364
BspOI GCTAGC 1 cut(s) 60
BspPI GGATC 3 cut(s) 9, 22, 113
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 3 cut(s) 14, 67, 105
BssSI CACGAG 1 cut(s) 109
BssT1I CCWWGG 1 cut(s) 101
Bst2BI CACGAG 1 cut(s) 109
Bst6I CTCTTC 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 58, 309
BstDEI CTNAG 4 cut(s) 61, 144, 295, 424
BstEII GGTNACC 1 cut(s) 329
BstF5I GGATG 2 cut(s) 277, 411
BstKTI GATC 3 cut(s) 17, 70, 108
BstMBI GATC 3 cut(s) 14, 67, 105
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstPI GGTNACC 1 cut(s) 329
BstV1I GCAGC 2 cut(s) 121, 364
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
BsuRI GGCC 2 cut(s) 322, 402
BtsCI GGATG 2 cut(s) 277, 411
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 2 cut(s) 58, 309
Cfr13I GGNCC 3 cut(s) 210, 320, 363
CviAII CATG 2 cut(s) 229, 341
DdeI CTNAG 4 cut(s) 61, 144, 295, 424
DpnI GATC 3 cut(s) 16, 69, 107
DpnII GATC 3 cut(s) 14, 67, 105
DraIII CACNNNGTG 1 cut(s) 232
DriI GACNNNNNGTC 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 102
Eam1105I GACNNNNNGTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 101
Eco47I GGWCC 2 cut(s) 210, 363
Eco91I GGTNACC 1 cut(s) 329
EcoO109I RGGNCCY 2 cut(s) 210, 363
EcoO65I GGTNACC 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 101
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 2 cut(s) 232, 344
FalI AAGNNNNNCTT 4 cut(s) 57, 89, 137, 169
FaqI GGGAC 3 cut(s) 181, 223, 376
FatI CATG 2 cut(s) 228, 340
Fnu4HI GCNGC 2 cut(s) 135, 378
FokI GGATG 2 cut(s) 284, 398
Fsp4HI GCNGC 2 cut(s) 135, 378
FspBI CTAG 2 cut(s) 57, 312
GluI GCNGC 2 cut(s) 135, 378
HaeIII GGCC 2 cut(s) 322, 402
Hin1II CATG 2 cut(s) 232, 344
HindIII AAGCTT 1 cut(s) 195
HphI GGTGA 2 cut(s) 244, 341
Hpy188I TCNGA 1 cut(s) 298
Hpy188III TCNNGA 2 cut(s) 109, 242
HpyAV CCTTC 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 1 cut(s) 78
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 4 cut(s) 61, 144, 295, 424
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 2 cut(s) 232, 344
KflI GGGWCCC 1 cut(s) 210
Kzo9I GATC 3 cut(s) 14, 67, 105
LpnPI CCDG 7 cut(s) 67, 156, 192, 227, 345, 384, 396
Lsp1109I GCAGC 2 cut(s) 121, 364
MaeI CTAG 2 cut(s) 57, 312
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 4 cut(s) 114, 224, 329, 419
MalI GATC 3 cut(s) 16, 69, 107
MboI GATC 3 cut(s) 14, 67, 105
MboII GAAGA 2 cut(s) 77, 89
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 267
MmeI TCCRAC 2 cut(s) 42, 282
MnlI CCTC 5 cut(s) 4, 105, 105, 265, 268
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 3 cut(s) 14, 67, 105
NheI GCTAGC 1 cut(s) 56
NlaIII CATG 2 cut(s) 232, 344
NlaIV GGNNCC 4 cut(s) 16, 211, 212, 364
NmuCI GTSAC 3 cut(s) 224, 329, 419
PkrI GCNGC 2 cut(s) 136, 379
PpuMI RGGWCCY 2 cut(s) 210, 363
Psp5II RGGWCCY 2 cut(s) 210, 363
PspEI GGTNACC 1 cut(s) 329
PspN4I GGNNCC 4 cut(s) 16, 211, 212, 364
PspPI GGNCC 3 cut(s) 210, 320, 363
PspPPI RGGWCCY 2 cut(s) 210, 363
PsuI RGATCY 1 cut(s) 14
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 2 cut(s) 135, 378
Sau3AI GATC 3 cut(s) 14, 67, 105
Sau96I GGNCC 3 cut(s) 210, 320, 363
SduI GDGCHC 1 cut(s) 267
SetI ASST 9 cut(s) 26, 58, 62, 116, 145, 199, 205, 264, 368
SinI GGWCC 2 cut(s) 210, 363
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
SspMI CTAG 2 cut(s) 57, 312
StyI CCWWGG 1 cut(s) 101
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 241, 347
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 360
TseFI GTSAC 3 cut(s) 224, 329, 419
TseI GCWGC 2 cut(s) 134, 377
Tsp45I GTSAC 3 cut(s) 224, 329, 419
TspRI CASTG 1 cut(s) 360
VpaK11BI GGWCC 2 cut(s) 210, 363
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XspI CTAG 2 cut(s) 57, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.