Rroxscaffold_3G00267540

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
N/A
Physical Location & Seq
Forward (+)
60611966 .. 60620410
8445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267540.1

Sequence Viewer

Length: 555 bp
ATGAGTACTTCTATGACGAATCTGGGATCCTCTTCCTCTTCTTCCTTCACTCATTCATGGACATATCATGTCTTCTTGAGCTTTAGAGGTAAGGATACACGCAACGGTTTCACAGGCCACTTGTACAAGGCTCTAGTTGATAAGGGAATTTACACATTCATTGATGAAAGCAAACTTAAAAGAGTTGAAGAAATATCAACAGCACTTCTCGAAGCTTCACCTGTGAGCCTGCCCCTGCAGCACCTACGCGCCCCTGCGCACGTATCCCTGCTGCCCTTGCAGCACCGCTGCACATCTGCTGCCCCTGCAGCACCAACGCACACTAACTGCATCCTTCTCCTTTCCTGTGCACGTCTGTCTTCTTACAAGCCCCAAAACTTCTCGTTCGGAGTCTCAGATCAAAATCCTTTCTTCATCAAAGTTGTTCCTCTCTGTCTCTTCCATCTGACCTCCAAATTTCAGCCTTATCGGAGTTGTTTTGAGACCGGTACACCAATCGAACTGCAAGATGTTCAGAAGAGAATCTGTTCCGAATTTCAACAAGTAAGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

184

Amino Acids

20.6

Weight (kDa)

8.96

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 72 6.2e-12 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 258
AccII CGCG 1 cut(s) 249
AciI CCGC 1 cut(s) 286
AclWI GGATC 2 cut(s) 21, 34
AcsI RAATTY 3 cut(s) 147, 455, 533
AfaI GTAC 3 cut(s) 7, 125, 490
AgeI ACCGGT 1 cut(s) 485
AgsI TTSAA 2 cut(s) 188, 539
AjiI CACGTC 1 cut(s) 353
AluBI AGCT 2 cut(s) 81, 215
AluI AGCT 2 cut(s) 81, 215
Alw21I GWGCWC 1 cut(s) 352
Alw26I GTCTC 3 cut(s) 397, 440, 476
Alw44I GTGCAC 1 cut(s) 348
AlwI GGATC 2 cut(s) 21, 34
AoxI GGCC 1 cut(s) 115
ApaLI GTGCAC 1 cut(s) 348
ApeKI GCWGC 6 cut(s) 238, 271, 280, 288, 299, 308
ApoI RAATTY 3 cut(s) 147, 455, 533
AsiGI ACCGGT 1 cut(s) 485
Asp700I GAANNNNTTC 1 cut(s) 526
AspLEI GCGC 2 cut(s) 251, 259
AsuHPI GGTGA 1 cut(s) 210
BaeGI GKGCMC 1 cut(s) 352
BamHI GGATCC 1 cut(s) 26
BbsI GAAGAC 2 cut(s) 64, 351
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 6 cut(s) 250, 258, 275, 286, 292, 320
BccI CCATC 1 cut(s) 450
BciVI GTATCC 2 cut(s) 88, 274
BcoDI GTCTC 3 cut(s) 397, 440, 476
BfaI CTAG 1 cut(s) 134
BfmI CTRYAG 2 cut(s) 236, 306
BfuI GTATCC 2 cut(s) 88, 274
BisI GCNGC 6 cut(s) 239, 272, 281, 289, 300, 309
BlsI GCNGC 6 cut(s) 240, 273, 282, 290, 301, 310
BmcAI AGTACT 1 cut(s) 7
BmgBI CACGTC 1 cut(s) 353
BmiI GGNNCC 1 cut(s) 28
BmsI GCATC 1 cut(s) 339
BpiI GAAGAC 2 cut(s) 64, 351
BpuEI CTTGAG 1 cut(s) 97
BsaAI YACGTR 1 cut(s) 262
BsaBI GATNNNNATC 1 cut(s) 402
BsaI GGTCTC 1 cut(s) 476
BsaWI WCCGGW 1 cut(s) 485
Bse118I RCCGGY 1 cut(s) 485
Bse8I GATNNNNATC 1 cut(s) 402
BseGI GGATG 1 cut(s) 330
BseJI GATNNNNATC 1 cut(s) 402
BseMII CTCAG 1 cut(s) 408
BseSI GKGCMC 1 cut(s) 352
BseXI GCAGC 6 cut(s) 250, 258, 275, 286, 292, 320
BsgI GTGCAG 1 cut(s) 274
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 1 cut(s) 117
BshTI ACCGGT 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 352
BsiSI CCGG 1 cut(s) 486
BsmAI GTCTC 3 cut(s) 397, 440, 476
BsnI GGCC 1 cut(s) 117
Bso31I GGTCTC 1 cut(s) 476
Bsp1286I GDGCHC 1 cut(s) 352
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 2 cut(s) 26, 397
BspACI CCGC 1 cut(s) 286
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 407
BspFNI CGCG 1 cut(s) 249
BspLI GGNNCC 1 cut(s) 28
BspMAI CTGCAG 2 cut(s) 240, 310
BspPI GGATC 2 cut(s) 21, 34
BspTNI GGTCTC 1 cut(s) 476
BsrFI RCCGGY 1 cut(s) 485
BsrGI TGTACA 1 cut(s) 123
BssAI RCCGGY 1 cut(s) 485
BssMI GATC 2 cut(s) 26, 397
Bst4CI ACNGT 1 cut(s) 107
Bst6I CTCTTC 4 cut(s) 37, 43, 443, 512
BstAUI TGTACA 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 262
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 330
BstFNI CGCG 1 cut(s) 249
BstHHI GCGC 2 cut(s) 251, 259
BstKTI GATC 2 cut(s) 29, 400
BstMAI GTCTC 3 cut(s) 397, 440, 476
BstMBI GATC 2 cut(s) 26, 397
BstMWI GCNNNNNNNGC 5 cut(s) 238, 277, 280, 305, 308
BstSFI CTRYAG 2 cut(s) 236, 306
BstSLI GKGCMC 1 cut(s) 352
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 6 cut(s) 250, 258, 275, 286, 292, 320
BstV2I GAAGAC 2 cut(s) 64, 351
BstX2I RGATCY 1 cut(s) 26
BstYI RGATCY 1 cut(s) 26
BsuI GTATCC 2 cut(s) 88, 274
BsuRI GGCC 1 cut(s) 117
BtrI CACGTC 1 cut(s) 353
BtsCI GGATG 1 cut(s) 330
Cac8I GCNNGC 1 cut(s) 230
CfoI GCGC 2 cut(s) 251, 259
Cfr10I RCCGGY 1 cut(s) 485
Csp6I GTAC 3 cut(s) 6, 124, 489
CspAI ACCGGT 1 cut(s) 485
CviAII CATG 2 cut(s) 57, 68
CviJI RGCY 7 cut(s) 81, 117, 131, 215, 228, 370, 463
CviKI_1 RGCY 7 cut(s) 81, 117, 131, 215, 228, 370, 463
CviQI GTAC 3 cut(s) 6, 124, 489
DdeI CTNAG 1 cut(s) 394
DpnI GATC 2 cut(s) 28, 399
DpnII GATC 2 cut(s) 26, 397
Eam1104I CTCTTC 4 cut(s) 37, 43, 443, 512
EarI CTCTTC 4 cut(s) 37, 43, 443, 512
Eco31I GGTCTC 1 cut(s) 476
FaeI CATG 2 cut(s) 60, 71
FaiI YATR 4 cut(s) 14, 58, 64, 69
FatI CATG 2 cut(s) 56, 67
Fnu4HI GCNGC 6 cut(s) 239, 272, 281, 289, 300, 309
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 6 cut(s) 239, 272, 281, 289, 300, 309
FspBI CTAG 1 cut(s) 134
FspI TGCGCA 1 cut(s) 258
GlaI GCGC 2 cut(s) 250, 258
GluI GCNGC 6 cut(s) 239, 272, 281, 289, 300, 309
HaeIII GGCC 1 cut(s) 117
HapII CCGG 1 cut(s) 486
HhaI GCGC 2 cut(s) 251, 259
Hin1II CATG 2 cut(s) 60, 71
Hin6I GCGC 2 cut(s) 249, 257
HinP1I GCGC 2 cut(s) 249, 257
HindIII AAGCTT 1 cut(s) 213
HinfI GANTC 3 cut(s) 19, 390, 522
HpaII CCGG 1 cut(s) 486
HphI GGTGA 1 cut(s) 210
Hpy166II GTNNAC 2 cut(s) 350, 491
Hpy188I TCNGA 6 cut(s) 389, 397, 447, 471, 516, 532
Hpy188III TCNNGA 2 cut(s) 76, 209
Hpy8I GTNNAC 2 cut(s) 350, 491
HpyAV CCTTC 2 cut(s) 55, 344
HpyCH4III ACNGT 1 cut(s) 107
HpyCH4IV ACGT 2 cut(s) 261, 352
HpyCH4V TGCA 7 cut(s) 238, 280, 291, 308, 330, 350, 505
HpyF10VI GCNNNNNNNGC 5 cut(s) 238, 277, 280, 305, 308
HpyF3I CTNAG 1 cut(s) 394
HpySE526I ACGT 2 cut(s) 261, 352
Hsp92II CATG 2 cut(s) 60, 71
HspAI GCGC 2 cut(s) 249, 257
Kzo9I GATC 2 cut(s) 26, 397
Lsp1109I GCAGC 6 cut(s) 250, 258, 275, 286, 292, 320
LweI GCATC 1 cut(s) 339
MaeI CTAG 1 cut(s) 134
MaeII ACGT 2 cut(s) 261, 352
MalI GATC 2 cut(s) 28, 399
MboI GATC 2 cut(s) 26, 397
MboII GAAGA 9 cut(s) 24, 30, 33, 64, 200, 351, 403, 430, 529
MflI RGATCY 1 cut(s) 26
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 3 cut(s) 147, 455, 533
MlyI GAGTC 1 cut(s) 399
MnlI CCTC 5 cut(s) 40, 46, 80, 438, 460
MroXI GAANNNNTTC 1 cut(s) 526
MseI TTAA 2 cut(s) 177, 553
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 1 cut(s) 486
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 5 cut(s) 238, 277, 280, 305, 308
NdeII GATC 2 cut(s) 26, 397
NlaIII CATG 2 cut(s) 60, 71
NlaIV GGNNCC 1 cut(s) 28
NsbI TGCGCA 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 526
PfeI GAWTC 2 cut(s) 19, 522
PinAI ACCGGT 1 cut(s) 485
PkrI GCNGC 6 cut(s) 240, 273, 282, 290, 301, 310
PleI GAGTC 1 cut(s) 398
PpsI GAGTC 1 cut(s) 398
Ppu21I YACGTR 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 28
PstI CTGCAG 2 cut(s) 240, 310
PsuI RGATCY 1 cut(s) 26
RsaI GTAC 3 cut(s) 7, 125, 490
RsaNI GTAC 3 cut(s) 6, 124, 489
SaqAI TTAA 2 cut(s) 177, 553
SatI GCNGC 6 cut(s) 239, 272, 281, 289, 300, 309
Sau3AI GATC 2 cut(s) 26, 397
ScaI AGTACT 1 cut(s) 7
SchI GAGTC 1 cut(s) 399
SduI GDGCHC 1 cut(s) 352
SetI ASST 8 cut(s) 83, 91, 217, 223, 246, 264, 355, 452
SfaNI GCATC 1 cut(s) 339
SfcI CTRYAG 2 cut(s) 236, 306
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
Sse9I AATT 3 cut(s) 147, 455, 533
SsiI CCGC 1 cut(s) 286
SspMI CTAG 1 cut(s) 134
TaaI ACNGT 1 cut(s) 107
TaiI ACGT 2 cut(s) 264, 355
TaqI TCGA 2 cut(s) 210, 498
TasI AATT 3 cut(s) 147, 455, 533
TatI WGTACW 2 cut(s) 5, 123
TfiI GAWTC 2 cut(s) 19, 522
Tru1I TTAA 2 cut(s) 177, 553
Tru9I TTAA 2 cut(s) 177, 553
TseI GCWGC 6 cut(s) 238, 271, 280, 288, 299, 308
TspDTI ATGAA 4 cut(s) 45, 148, 180, 403
VneI GTGCAC 1 cut(s) 348
XapI RAATTY 3 cut(s) 147, 455, 533
XmnI GAANNNNTTC 1 cut(s) 526
XspI CTAG 1 cut(s) 134
ZrmI AGTACT 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.