Rroxscaffold_4G00294020

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
N/A
Physical Location & Seq
Reverse (-)
14197483 .. 14198181
699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00294020.1

Sequence Viewer

Length: 699 bp
ATGGCGATTAATCCGGCGGAGTTTGTTGGAGGGGCTGGTCTAGGATCAGCGTTTAGTTTACTGACCAGTGGCGTTCAGGAGCTGCTAAGCAAGGCTGCCATGTTTGAGCCTCTCCTCAAAAGAATACAACCCACGCTAATCGCCTTGGATTCACAGGTCCCAGATACTGAAGAGGAGCGGAAAGGTTTAGAAGAAGTGAGAAAGATGGGAGAAAAGCTTGTCCGGGATTGCAAAGAAGTTAGTGAGTGGAACCCGTTCGCGAAGTACGCCGCCGCCGTCAAGCTTTTGGAATGGAATGATTCTCTTAAACACCAATTATGGATACACAAATTAAAGAAAGGAACGAAGACCGCGTCTACGTTTGAGAAGATCGCTTCTACGGCTGAGAAGACTGCTACATCGGTAGATAGTCTGCTGGATTCAGTAAAGATACTAAAGAATCGTGTGATACTGAATAAAGCCGCAACAAGTTCAAGTATTCCAGCTCTTCGACCTCCAGGGTTTCCACAACATATAGTTGGATTGGATACTCCTTTGATGGAATTGAAGGAAATACTTCTTAAGGATCATGGACTCTCGATGCTTGTGCTGACTGCTCCACCAGGATGTGGGAAAACTACTCTGGCCGCAATGTTTTGTGAAGACAAAGAAGTAAAAGGTACGGTTCATGTAGATTTAATGATTTATAGATCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

232

Amino Acids

25.42

Weight (kDa)

8.66

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 104 3.6e-09 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 608
AccBSI CCGCTC 1 cut(s) 178
AccI GTMKAC 1 cut(s) 356
AccII CGCG 2 cut(s) 260, 353
AciI CCGC 7 cut(s) 17, 178, 270, 273, 351, 462, 627
AclWI GGATC 3 cut(s) 52, 573, 684
AcoI YGGCCR 1 cut(s) 624
AcuI CTGAAG 1 cut(s) 189
AfaI GTAC 2 cut(s) 266, 661
AfiI CCNNNNNNNGG 1 cut(s) 608
AflII CTTAAG 1 cut(s) 560
AgsI TTSAA 2 cut(s) 474, 547
AjnI CCWGG 2 cut(s) 496, 601
AluBI AGCT 4 cut(s) 82, 217, 283, 485
AluI AGCT 4 cut(s) 82, 217, 283, 485
AlwI GGATC 3 cut(s) 52, 573, 684
AlwNI CAGNNNCTG 2 cut(s) 82, 167
AoxI GGCC 1 cut(s) 624
ApeKI GCWGC 2 cut(s) 82, 95
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
AseI ATTAAT 1 cut(s) 9
Asp700I GAANNNNTTC 2 cut(s) 254, 555
AspS9I GGNCC 1 cut(s) 157
AsuC2I CCSGG 1 cut(s) 224
AvaII GGWCC 1 cut(s) 157
BbsI GAAGAC 3 cut(s) 353, 395, 648
BbvI GCAGC 2 cut(s) 69, 82
BccI CCATC 2 cut(s) 199, 532
BceAI ACGGC 2 cut(s) 260, 396
BcgI CGANNNNNNTGC 2 cut(s) 568, 602
BciT130I CCWGG 2 cut(s) 498, 603
BciVI GTATCC 2 cut(s) 315, 520
BcnI CCSGG 1 cut(s) 224
BfaI CTAG 1 cut(s) 41
BfrI CTTAAG 1 cut(s) 560
BfuI GTATCC 2 cut(s) 315, 520
BisI GCNGC 6 cut(s) 83, 96, 270, 273, 462, 627
BlpI GCTNAGC 1 cut(s) 86
BlsI GCNGC 6 cut(s) 84, 97, 271, 274, 463, 628
Bme1390I CCNGG 3 cut(s) 224, 498, 603
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 2 cut(s) 159, 251
BmrFI CCNGG 3 cut(s) 224, 498, 603
BmsI GCATC 1 cut(s) 570
BpiI GAAGAC 3 cut(s) 353, 395, 648
BpmI CTGGAG 1 cut(s) 480
Bpu1102I GCTNAGC 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 144, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 608
Bse1I ACTGG 1 cut(s) 66
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 2 cut(s) 498, 603
BseDI CCNNGG 2 cut(s) 144, 497
BseGI GGATG 1 cut(s) 611
BseLI CCNNNNNNNGG 1 cut(s) 608
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 66
BseRI GAGGAG 2 cut(s) 104, 188
BseXI GCAGC 2 cut(s) 69, 82
Bsh1236I CGCG 2 cut(s) 260, 353
BshFI GGCC 1 cut(s) 626
BsiSI CCGG 2 cut(s) 14, 223
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 608
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 1 cut(s) 626
Bsp143I GATC 4 cut(s) 44, 369, 565, 689
Bsp1720I GCTNAGC 1 cut(s) 86
Bsp68I TCGCGA 1 cut(s) 260
BspACI CCGC 7 cut(s) 17, 178, 270, 273, 351, 462, 627
BspANI GGCC 1 cut(s) 626
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 2 cut(s) 260, 353
BspLI GGNNCC 2 cut(s) 159, 251
BspPI GGATC 3 cut(s) 52, 573, 684
BspQI GCTCTTC 1 cut(s) 492
BspTI CTTAAG 1 cut(s) 560
BsrBI CCGCTC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 1 cut(s) 66
BssECI CCNNGG 2 cut(s) 144, 497
BssMI GATC 4 cut(s) 44, 369, 565, 689
BssT1I CCWWGG 1 cut(s) 144
Bst2UI CCWGG 2 cut(s) 498, 603
Bst4CI ACNGT 1 cut(s) 664
Bst6I CTCTTC 2 cut(s) 165, 492
BstAFI CTTAAG 1 cut(s) 560
BstDEI CTNAG 2 cut(s) 86, 384
BstF5I GGATG 1 cut(s) 611
BstFNI CGCG 2 cut(s) 260, 353
BstKTI GATC 4 cut(s) 47, 372, 568, 692
BstMBI GATC 4 cut(s) 44, 369, 565, 689
BstMWI GCNNNNNNNGC 2 cut(s) 266, 380
BstNI CCWGG 2 cut(s) 498, 603
BstSCI CCNGG 3 cut(s) 222, 496, 601
BstUI CGCG 2 cut(s) 260, 353
BstV1I GCAGC 2 cut(s) 69, 82
BstV2I GAAGAC 3 cut(s) 353, 395, 648
BstX2I RGATCY 1 cut(s) 689
BstYI RGATCY 1 cut(s) 689
BsuI GTATCC 2 cut(s) 315, 520
BsuRI GGCC 1 cut(s) 626
BtsCI GGATG 1 cut(s) 611
BtsIMutI CAGTG 1 cut(s) 73
BtuMI TCGCGA 1 cut(s) 260
CaiI CAGNNNCTG 2 cut(s) 82, 167
Cfr13I GGNCC 1 cut(s) 157
CseI GACGC 1 cut(s) 342
Csp6I GTAC 2 cut(s) 265, 660
CviAII CATG 3 cut(s) 100, 569, 668
CviQI GTAC 2 cut(s) 265, 660
DdeI CTNAG 2 cut(s) 86, 384
DpnI GATC 4 cut(s) 46, 371, 567, 691
DpnII GATC 4 cut(s) 44, 369, 565, 689
EaeI YGGCCR 1 cut(s) 624
Eam1104I CTCTTC 2 cut(s) 165, 492
EarI CTCTTC 2 cut(s) 165, 492
EciI GGCGGA 1 cut(s) 32
Eco130I CCWWGG 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 496, 601
EcoT14I CCWWGG 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 3 cut(s) 103, 572, 671
FaiI YATR 7 cut(s) 101, 319, 513, 515, 570, 669, 687
FaqI GGGAC 1 cut(s) 143
FatI CATG 3 cut(s) 99, 568, 667
FblI GTMKAC 1 cut(s) 356
Fnu4HI GCNGC 6 cut(s) 83, 96, 270, 273, 462, 627
FokI GGATG 1 cut(s) 618
Fsp4HI GCNGC 6 cut(s) 83, 96, 270, 273, 462, 627
FspBI CTAG 1 cut(s) 41
GluI GCNGC 6 cut(s) 83, 96, 270, 273, 462, 627
GsuI CTGGAG 1 cut(s) 480
HaeIII GGCC 1 cut(s) 626
HapII CCGG 2 cut(s) 14, 223
HgaI GACGC 1 cut(s) 342
Hin1II CATG 3 cut(s) 103, 572, 671
HindIII AAGCTT 2 cut(s) 215, 281
HinfI GANTC 5 cut(s) 149, 299, 419, 439, 573
HpaII CCGG 2 cut(s) 14, 223
Hpy166II GTNNAC 2 cut(s) 59, 357
Hpy188III TCNNGA 4 cut(s) 77, 259, 577, 693
Hpy8I GTNNAC 2 cut(s) 59, 357
HpyAV CCTTC 1 cut(s) 541
HpyCH4III ACNGT 1 cut(s) 664
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 380
HpyF3I CTNAG 2 cut(s) 86, 384
HpySE526I ACGT 1 cut(s) 359
Hsp92II CATG 3 cut(s) 103, 572, 671
Kzo9I GATC 4 cut(s) 44, 369, 565, 689
LguI GCTCTTC 1 cut(s) 492
LmnI GCTCC 3 cut(s) 79, 175, 601
Lsp1109I GCAGC 2 cut(s) 69, 82
LweI GCATC 1 cut(s) 570
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 359
MalI GATC 4 cut(s) 46, 371, 567, 691
MbiI CCGCTC 1 cut(s) 178
MboI GATC 4 cut(s) 44, 369, 565, 689
MboII GAAGA 7 cut(s) 182, 203, 358, 379, 400, 479, 653
MflI RGATCY 1 cut(s) 689
MluCI AATT 3 cut(s) 314, 329, 542
MlyI GAGTC 1 cut(s) 567
MmeI TCCRAC 2 cut(s) 7, 499
MnlI CCTC 5 cut(s) 23, 120, 125, 166, 504
MroXI GAANNNNTTC 2 cut(s) 254, 555
MseI TTAA 5 cut(s) 9, 306, 332, 561, 677
MslI CAYNNNNRTG 1 cut(s) 604
MspCI CTTAAG 1 cut(s) 560
MspI CCGG 2 cut(s) 14, 223
MspR9I CCNGG 3 cut(s) 224, 498, 603
MvaI CCWGG 2 cut(s) 498, 603
MvnI CGCG 2 cut(s) 260, 353
MwoI GCNNNNNNNGC 2 cut(s) 266, 380
NciI CCSGG 1 cut(s) 224
NdeII GATC 4 cut(s) 44, 369, 565, 689
NlaIII CATG 3 cut(s) 103, 572, 671
NlaIV GGNNCC 2 cut(s) 159, 251
NruI TCGCGA 1 cut(s) 260
PciSI GCTCTTC 1 cut(s) 492
PcsI WCGNNNNNNNCGW 2 cut(s) 273, 350
PdmI GAANNNNTTC 2 cut(s) 254, 555
PfeI GAWTC 4 cut(s) 149, 299, 419, 439
PflFI GACNNNGTC 1 cut(s) 352
PflMI CCANNNNNTGG 1 cut(s) 608
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 6 cut(s) 84, 97, 271, 274, 463, 628
PleI GAGTC 1 cut(s) 567
PpsI GAGTC 1 cut(s) 567
PpuMI RGGWCCY 1 cut(s) 157
PshBI ATTAAT 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 2 cut(s) 496, 601
PspGI CCWGG 2 cut(s) 496, 601
PspN4I GGNNCC 2 cut(s) 159, 251
PspPI GGNCC 1 cut(s) 157
PspPPI RGGWCCY 1 cut(s) 157
PstNI CAGNNNCTG 2 cut(s) 82, 167
PsuI RGATCY 1 cut(s) 689
PsyI GACNNNGTC 1 cut(s) 352
RruI TCGCGA 1 cut(s) 260
RsaI GTAC 2 cut(s) 266, 661
RsaNI GTAC 2 cut(s) 265, 660
RseI CAYNNNNRTG 1 cut(s) 604
SapI GCTCTTC 1 cut(s) 492
SaqAI TTAA 5 cut(s) 9, 306, 332, 561, 677
SatI GCNGC 6 cut(s) 83, 96, 270, 273, 462, 627
Sau3AI GATC 4 cut(s) 44, 369, 565, 689
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 1 cut(s) 567
ScrFI CCNGG 3 cut(s) 224, 498, 603
SetI ASST 9 cut(s) 84, 159, 187, 219, 285, 362, 487, 496, 661
SfaNI GCATC 1 cut(s) 570
SinI GGWCC 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 604
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 3 cut(s) 314, 329, 542
SsiI CCGC 7 cut(s) 17, 178, 270, 273, 351, 462, 627
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 3 cut(s) 222, 496, 601
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 1 cut(s) 664
TaiI ACGT 1 cut(s) 362
TaqI TCGA 2 cut(s) 490, 578
TasI AATT 3 cut(s) 314, 329, 542
TauI GCSGC 4 cut(s) 272, 275, 464, 629
TfiI GAWTC 4 cut(s) 149, 299, 419, 439
Tru1I TTAA 5 cut(s) 9, 306, 332, 561, 677
Tru9I TTAA 5 cut(s) 9, 306, 332, 561, 677
TscAI CASTG 1 cut(s) 73
TseI GCWGC 2 cut(s) 82, 95
TspDTI ATGAA 1 cut(s) 656
TspRI CASTG 1 cut(s) 73
Tth111I GACNNNGTC 1 cut(s) 352
Van91I CCANNNNNTGG 1 cut(s) 608
Vha464I CTTAAG 1 cut(s) 560
VpaK11BI GGWCC 1 cut(s) 157
VspI ATTAAT 1 cut(s) 9
XmiI GTMKAC 1 cut(s) 356
XmnI GAANNNNTTC 2 cut(s) 254, 555
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.