Rroxscaffold_4G00306790

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
N/A
Physical Location & Seq
Reverse (-)
27746272 .. 27757404
11133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306790.1

Sequence Viewer

Length: 483 bp
ATGGCTCCACCAAGGTCACCTTCACCATCCGGGCGCTCTACCCCCACGGGTCCACCAATTGGTCTGTCTTCACATGTTGCGGACCCCACTTGTCCACTACCATTTTTGTCTTCGCAGGATGCGGACCCCACCGGATTTGTGCAACTTGGGATCCATGGAAGCAATTATGATGTCCACCCCGGACATGCTATGTTGCGATTGGGTGTCCAACAACCCCCCCCCCCAAAATCCAAGGAGGACTACATGAATATGGTATTTTCAGCACATTTCTTGCTGGAAAAGACAACCAGGTTCCAGGCCAGTTCAGTTAAAGAAGTAAAAGGGCCCTCAATATCTTTTACTGTGCCTTTACTTCCTCCTAATCTCAAAATCAGAGGCTTGAACATCTTATCTGTTTATGAAAGGAGGTCCCCCATGATTTCCTTCCCTATTGGTTTCCTGAATCCACTAATTATTAGAATCAGGAATAAGAGCAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

160

Amino Acids

17.45

Weight (kDa)

10.07

Isoelectric Point (pI)

62.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 59
AciI CCGC 2 cut(s) 80, 122
AclWI GGATC 2 cut(s) 145, 158
AfiI CCNNNNNNNGG 1 cut(s) 59
AflIII ACRYGT 1 cut(s) 73
AgsI TTSAA 1 cut(s) 382
AjnI CCWGG 2 cut(s) 287, 294
AjuI GAANNNNNNNTTGG 1 cut(s) 36
AlwI GGATC 2 cut(s) 145, 158
AoxI GGCC 2 cut(s) 297, 323
ApaI GGGCCC 1 cut(s) 327
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 6 cut(s) 50, 82, 124, 323, 324, 408
AsuC2I CCSGG 2 cut(s) 31, 180
AsuHPI GGTGA 2 cut(s) 9, 15
AvaII GGWCC 4 cut(s) 50, 82, 124, 408
BaeGI GKGCMC 1 cut(s) 327
BamHI GGATCC 1 cut(s) 150
BanII GRGCYC 1 cut(s) 327
BbsI GAAGAC 2 cut(s) 60, 102
BccI CCATC 1 cut(s) 34
BciT130I CCWGG 2 cut(s) 289, 296
BcnI CCSGG 2 cut(s) 31, 180
BfoI RGCGCY 1 cut(s) 37
Bme1390I CCNGG 4 cut(s) 31, 180, 289, 296
Bme18I GGWCC 4 cut(s) 50, 82, 124, 408
BmgT120I GGNCC 6 cut(s) 50, 82, 124, 323, 324, 408
BmiI GGNNCC 8 cut(s) 6, 51, 84, 126, 152, 293, 325, 410
BmrFI CCNGG 4 cut(s) 31, 180, 289, 296
BmsI GCATC 1 cut(s) 109
BpiI GAAGAC 2 cut(s) 60, 102
BpuMI CCSGG 2 cut(s) 31, 180
BsaJI CCNNGG 5 cut(s) 11, 45, 154, 178, 231
BsaWI WCCGGW 1 cut(s) 131
Bsc4I CCNNNNNNNGG 1 cut(s) 59
Bse1I ACTGG 1 cut(s) 300
BseBI CCWGG 2 cut(s) 289, 296
BseDI CCNNGG 5 cut(s) 11, 45, 154, 178, 231
BseGI GGATG 2 cut(s) 26, 124
BseLI CCNNNNNNNGG 1 cut(s) 59
BseNI ACTGG 1 cut(s) 300
BseSI GKGCMC 1 cut(s) 327
BshFI GGCC 2 cut(s) 299, 325
BsiSI CCGG 3 cut(s) 30, 132, 180
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 1 cut(s) 59
BsmFI GGGAC 1 cut(s) 394
BsnI GGCC 2 cut(s) 299, 325
Bsp120I GGGCCC 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 1 cut(s) 150
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 2 cut(s) 80, 122
BspANI GGCC 2 cut(s) 299, 325
BspLI GGNNCC 8 cut(s) 6, 51, 84, 126, 152, 293, 325, 410
BspPI GGATC 2 cut(s) 145, 158
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 5 cut(s) 11, 45, 154, 178, 231
BssMI GATC 1 cut(s) 150
BssT1I CCWWGG 3 cut(s) 11, 154, 231
Bst2UI CCWGG 2 cut(s) 289, 296
Bst4CI ACNGT 1 cut(s) 343
BstDSI CCRYGG 2 cut(s) 45, 154
BstEII GGTNACC 1 cut(s) 15
BstF5I GGATG 2 cut(s) 26, 124
BstH2I RGCGCY 1 cut(s) 37
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 1 cut(s) 153
BstMBI GATC 1 cut(s) 150
BstNI CCWGG 2 cut(s) 289, 296
BstNSI RCATGY 2 cut(s) 77, 188
BstPI GGTNACC 1 cut(s) 15
BstSCI CCNGG 4 cut(s) 29, 178, 287, 294
BstSLI GKGCMC 1 cut(s) 327
BstV2I GAAGAC 2 cut(s) 60, 102
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BsuRI GGCC 2 cut(s) 299, 325
BtgI CCRYGG 2 cut(s) 45, 154
BtsCI GGATG 2 cut(s) 26, 124
CfoI GCGC 1 cut(s) 36
Cfr13I GGNCC 6 cut(s) 50, 82, 124, 323, 324, 408
CsiI ACCWGGT 1 cut(s) 287
CspCI CAANNNNNGTGG 2 cut(s) 118, 153
CviAII CATG 5 cut(s) 74, 155, 185, 244, 415
CviJI RGCY 4 cut(s) 5, 299, 325, 378
CviKI_1 RGCY 4 cut(s) 5, 299, 325, 378
DpnI GATC 1 cut(s) 152
DpnII GATC 1 cut(s) 150
Eco130I CCWWGG 3 cut(s) 11, 154, 231
Eco24I GRGCYC 1 cut(s) 327
Eco47I GGWCC 4 cut(s) 50, 82, 124, 408
Eco91I GGTNACC 1 cut(s) 15
EcoO109I RGGNCCY 3 cut(s) 323, 324, 408
EcoO65I GGTNACC 1 cut(s) 15
EcoRII CCWGG 2 cut(s) 287, 294
EcoT14I CCWWGG 3 cut(s) 11, 154, 231
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 3 cut(s) 11, 154, 231
FaeI CATG 5 cut(s) 77, 158, 188, 247, 418
FaiI YATR 9 cut(s) 75, 156, 168, 186, 191, 245, 251, 399, 416
FalI AAGNNNNNCTT 1 cut(s) 36
FaqI GGGAC 1 cut(s) 394
FatI CATG 5 cut(s) 73, 154, 184, 243, 414
FokI GGATG 2 cut(s) 13, 131
FriOI GRGCYC 1 cut(s) 327
GlaI GCGC 1 cut(s) 35
HaeII RGCGCY 1 cut(s) 37
HaeIII GGCC 2 cut(s) 299, 325
HapII CCGG 3 cut(s) 30, 132, 180
HhaI GCGC 1 cut(s) 36
Hin1II CATG 5 cut(s) 77, 158, 188, 247, 418
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HinfI GANTC 2 cut(s) 442, 459
HpaII CCGG 3 cut(s) 30, 132, 180
HphI GGTGA 2 cut(s) 9, 15
Hpy166II GTNNAC 3 cut(s) 53, 95, 175
Hpy188I TCNGA 1 cut(s) 374
Hpy188III TCNNGA 2 cut(s) 439, 463
Hpy8I GTNNAC 3 cut(s) 53, 95, 175
HpyAV CCTTC 2 cut(s) 30, 433
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4V TGCA 1 cut(s) 142
Hsp92II CATG 5 cut(s) 77, 158, 188, 247, 418
HspAI GCGC 1 cut(s) 34
Kzo9I GATC 1 cut(s) 150
LmnI GCTCC 1 cut(s) 10
LweI GCATC 1 cut(s) 109
MabI ACCWGGT 1 cut(s) 287
MaeIII GTNAC 1 cut(s) 15
MalI GATC 1 cut(s) 152
MboI GATC 1 cut(s) 150
MboII GAAGA 2 cut(s) 60, 102
MfeI CAATTG 1 cut(s) 57
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 3 cut(s) 57, 163, 450
MmeI TCCRAC 1 cut(s) 232
MnlI CCTC 5 cut(s) 229, 337, 366, 368, 399
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 248
MspI CCGG 3 cut(s) 30, 132, 180
MspR9I CCNGG 4 cut(s) 31, 180, 289, 296
MunI CAATTG 1 cut(s) 57
MvaI CCWGG 2 cut(s) 289, 296
NciI CCSGG 2 cut(s) 31, 180
NcoI CCATGG 1 cut(s) 154
NdeII GATC 1 cut(s) 150
NlaIII CATG 5 cut(s) 77, 158, 188, 247, 418
NlaIV GGNNCC 8 cut(s) 6, 51, 84, 126, 152, 293, 325, 410
NmuCI GTSAC 1 cut(s) 15
NspI RCATGY 2 cut(s) 77, 188
PciI ACATGT 1 cut(s) 73
PfeI GAWTC 2 cut(s) 442, 459
PflMI CCANNNNNTGG 1 cut(s) 59
PpuMI RGGWCCY 1 cut(s) 408
PscI ACATGT 1 cut(s) 73
Psp5II RGGWCCY 1 cut(s) 408
Psp6I CCWGG 2 cut(s) 287, 294
PspEI GGTNACC 1 cut(s) 15
PspGI CCWGG 2 cut(s) 287, 294
PspN4I GGNNCC 8 cut(s) 6, 51, 84, 126, 152, 293, 325, 410
PspOMI GGGCCC 1 cut(s) 323
PspPI GGNCC 6 cut(s) 50, 82, 124, 323, 324, 408
PspPPI RGGWCCY 1 cut(s) 408
PsuI RGATCY 1 cut(s) 150
RseI CAYNNNNRTG 1 cut(s) 248
SaqAI TTAA 1 cut(s) 309
Sau3AI GATC 1 cut(s) 150
Sau96I GGNCC 6 cut(s) 50, 82, 124, 323, 324, 408
ScrFI CCNGG 4 cut(s) 31, 180, 289, 296
SduI GDGCHC 1 cut(s) 327
SetI ASST 5 cut(s) 17, 22, 293, 410, 480
SexAI ACCWGGT 1 cut(s) 287
SfaNI GCATC 1 cut(s) 109
SinI GGWCC 4 cut(s) 50, 82, 124, 408
SmiMI CAYNNNNRTG 1 cut(s) 248
Sse9I AATT 3 cut(s) 57, 163, 450
SsiI CCGC 2 cut(s) 80, 122
StyD4I CCNGG 4 cut(s) 29, 178, 287, 294
StyI CCWWGG 3 cut(s) 11, 154, 231
TaaI ACNGT 1 cut(s) 343
TasI AATT 3 cut(s) 57, 163, 450
TfiI GAWTC 2 cut(s) 442, 459
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TseFI GTSAC 1 cut(s) 15
Tsp45I GTSAC 1 cut(s) 15
TspDTI ATGAA 2 cut(s) 260, 414
Van91I CCANNNNNTGG 1 cut(s) 59
VpaK11BI GGWCC 4 cut(s) 50, 82, 124, 408
XceI RCATGY 2 cut(s) 77, 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.