Rroxscaffold_4G00320100

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
N/A
Physical Location & Seq
Forward (+)
48484018 .. 48494413
10396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00320100.1

Sequence Viewer

Length: 561 bp
ATGCATTATGAAGAAGCGACCGTTCACTCCAAAGAAAGAGAATTTTCTTCGAGCTTCCACCACTTCCTCTCAACCTTCAACCTTTCTTTCGAAACTCAAATCCCTTCTTCACCACATCAATCCTTCTCTTCCTTATCTTTTCTTCCATCTCGGGAGGAAACTGCAGCAATAAGCTTGGAGAGACAAGTATATATATCAATGCTCAGCTTTTTGAGGTTGCTCATCTATCTTAGGAGAGGGGATCTCACCCCAACTTCGAAAGCAATTGAAGAGTCGAGAATCTCCATCATCATTTTCTCTAAGAACTACACATCATCAAGGTGGTGCTTGGATGAGCTCGTCAAAATTCTTGAGTGTAAAAATAAAAACCATCAAATGGTTTGGCCAATTTTTTACAAGGTGGATCCCTCACATGTGAGACACCAAACTAGTACTTTTGGTGATGCGTTTGTGGTTCATGAATGCAAATACAAGGACAACATGGAAAGAGTGCTCCTATGGAGAAAAGCTTTTGTGCAAGCAGCAAATTTGTCCGGGTGGACTTTCAAGAAGGGAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

186

Amino Acids

21.82

Weight (kDa)

8.75

Isoelectric Point (pI)

57.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 86 - 184 2.5e-32 TIR domain
TIR_2 PF13676 86 - 136 9.2e-07 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 376
AclWI GGATC 3 cut(s) 249, 398, 411
AcoI YGGCCR 1 cut(s) 383
AcsI RAATTY 3 cut(s) 41, 345, 526
AfaI GTAC 1 cut(s) 433
AfiI CCNNNNNNNGG 1 cut(s) 376
AflIII ACRYGT 1 cut(s) 412
AgsI TTSAA 3 cut(s) 79, 269, 547
AhlI ACTAGT 1 cut(s) 428
AluBI AGCT 5 cut(s) 54, 174, 207, 337, 509
AluI AGCT 5 cut(s) 54, 174, 207, 337, 509
Alw21I GWGCWC 2 cut(s) 339, 495
Alw26I GTCTC 2 cut(s) 175, 412
AlwI GGATC 3 cut(s) 249, 398, 411
Ama87I CYCGRG 1 cut(s) 150
AoxI GGCC 1 cut(s) 383
ApeKI GCWGC 2 cut(s) 164, 521
ApoI RAATTY 3 cut(s) 41, 345, 526
AsuC2I CCSGG 1 cut(s) 535
AsuHPI GGTGA 3 cut(s) 102, 238, 452
AsuII TTCGAA 2 cut(s) 90, 257
AvaI CYCGRG 1 cut(s) 150
BalI TGGCCA 1 cut(s) 385
BamHI GGATCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 2 cut(s) 339, 495
BbvI GCAGC 2 cut(s) 176, 533
BccI CCATC 3 cut(s) 154, 293, 378
BcnI CCSGG 1 cut(s) 535
BcoDI GTCTC 2 cut(s) 175, 412
BcuI ACTAGT 1 cut(s) 428
BfaI CTAG 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 162
BisI GCNGC 2 cut(s) 165, 522
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 2 cut(s) 166, 523
BmcAI AGTACT 1 cut(s) 433
Bme1390I CCNGG 1 cut(s) 535
BmeT110I CYCGRG 1 cut(s) 150
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 1 cut(s) 535
BmsI GCATC 1 cut(s) 433
BplI GAGNNNNNCTC 2 cut(s) 228, 260
Bpu1102I GCTNAGC 1 cut(s) 203
Bpu14I TTCGAA 2 cut(s) 90, 257
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 535
Bsc4I CCNNNNNNNGG 1 cut(s) 376
BseGI GGATG 1 cut(s) 337
BseLI CCNNNNNNNGG 1 cut(s) 376
BseMII CTCAG 1 cut(s) 217
BseXI GCAGC 2 cut(s) 176, 533
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 385
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 2 cut(s) 339, 495
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 1 cut(s) 534
BslI CCNNNNNNNGG 1 cut(s) 376
BsmAI GTCTC 2 cut(s) 175, 412
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 1 cut(s) 385
BsoBI CYCGRG 1 cut(s) 150
Bsp119I TTCGAA 2 cut(s) 90, 257
Bsp1286I GDGCHC 2 cut(s) 339, 495
Bsp143I GATC 2 cut(s) 241, 403
Bsp1720I GCTNAGC 1 cut(s) 203
BspANI GGCC 1 cut(s) 385
BspCNI CTCAG 1 cut(s) 216
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 1 cut(s) 405
BspMAI CTGCAG 1 cut(s) 166
BspPI GGATC 3 cut(s) 249, 398, 411
BspT104I TTCGAA 2 cut(s) 90, 257
BssMI GATC 2 cut(s) 241, 403
Bst4CI ACNGT 1 cut(s) 22
Bst6I CTCTTC 2 cut(s) 133, 264
BstBI TTCGAA 2 cut(s) 90, 257
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 3 cut(s) 203, 230, 300
BstF5I GGATG 1 cut(s) 337
BstKTI GATC 2 cut(s) 244, 406
BstMAI GTCTC 2 cut(s) 175, 412
BstMBI GATC 2 cut(s) 241, 403
BstMCI CGRYCG 1 cut(s) 21
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 533
BstSFI CTRYAG 1 cut(s) 162
BstV1I GCAGC 2 cut(s) 176, 533
BstX2I RGATCY 2 cut(s) 241, 403
BstYI RGATCY 2 cut(s) 241, 403
BsuRI GGCC 1 cut(s) 385
BtsCI GGATG 1 cut(s) 337
Cac8I GCNNGC 1 cut(s) 519
CciI TCATGA 1 cut(s) 457
Csp6I GTAC 1 cut(s) 432
CviAII CATG 3 cut(s) 413, 458, 481
CviJI RGCY 6 cut(s) 54, 174, 207, 337, 385, 509
CviKI_1 RGCY 6 cut(s) 54, 174, 207, 337, 385, 509
CviQI GTAC 1 cut(s) 432
DdeI CTNAG 3 cut(s) 203, 230, 300
DpnI GATC 2 cut(s) 243, 405
DpnII GATC 2 cut(s) 241, 403
EaeI YGGCCR 1 cut(s) 383
Eam1104I CTCTTC 2 cut(s) 133, 264
EarI CTCTTC 2 cut(s) 133, 264
Ecl136II GAGCTC 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 337
Eco88I CYCGRG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 337
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 339
FaeI CATG 3 cut(s) 416, 461, 484
FaiI YATR 8 cut(s) 9, 190, 192, 194, 414, 459, 482, 499
FatI CATG 3 cut(s) 412, 457, 480
Fnu4HI GCNGC 2 cut(s) 165, 522
FokI GGATG 1 cut(s) 344
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 2 cut(s) 165, 522
FspBI CTAG 1 cut(s) 429
GluI GCNGC 2 cut(s) 165, 522
HaeIII GGCC 1 cut(s) 385
HapII CCGG 1 cut(s) 534
Hin1II CATG 3 cut(s) 416, 461, 484
HindIII AAGCTT 2 cut(s) 172, 507
HinfI GANTC 2 cut(s) 272, 279
HpaII CCGG 1 cut(s) 534
HphI GGTGA 3 cut(s) 102, 238, 452
Hpy166II GTNNAC 2 cut(s) 25, 540
Hpy188III TCNNGA 5 cut(s) 152, 276, 350, 458, 547
Hpy8I GTNNAC 2 cut(s) 25, 540
HpyAV CCTTC 4 cut(s) 85, 114, 133, 544
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4V TGCA 4 cut(s) 4, 164, 465, 517
HpyF3I CTNAG 3 cut(s) 203, 230, 300
Hsp92II CATG 3 cut(s) 416, 461, 484
Kzo9I GATC 2 cut(s) 241, 403
LmnI GCTCC 1 cut(s) 498
LpnPI CCDG 1 cut(s) 547
Lsp1109I GCAGC 2 cut(s) 176, 533
LweI GCATC 1 cut(s) 433
MaeI CTAG 1 cut(s) 429
MalI GATC 2 cut(s) 243, 405
MboI GATC 2 cut(s) 241, 403
MboII GAAGA 6 cut(s) 23, 39, 99, 120, 134, 281
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 2 cut(s) 241, 403
MhlI GDGCHC 2 cut(s) 339, 495
MlsI TGGCCA 1 cut(s) 385
MluCI AATT 5 cut(s) 41, 264, 345, 387, 526
MluNI TGGCCA 1 cut(s) 385
MlyI GAGTC 1 cut(s) 281
MnlI CCTC 5 cut(s) 77, 148, 207, 230, 418
Mox20I TGGCCA 1 cut(s) 385
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 385
MslI CAYNNNNRTG 1 cut(s) 319
Msp20I TGGCCA 1 cut(s) 385
MspI CCGG 1 cut(s) 534
MspR9I CCNGG 1 cut(s) 535
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 467
NciI CCSGG 1 cut(s) 535
NdeII GATC 2 cut(s) 241, 403
NlaIII CATG 3 cut(s) 416, 461, 484
NlaIV GGNNCC 1 cut(s) 405
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 416
NspV TTCGAA 2 cut(s) 90, 257
PagI TCATGA 1 cut(s) 457
PciI ACATGT 1 cut(s) 412
PctI GAATGC 1 cut(s) 467
PfeI GAWTC 1 cut(s) 279
PflMI CCANNNNNTGG 1 cut(s) 376
PkrI GCNGC 2 cut(s) 166, 523
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
PscI ACATGT 1 cut(s) 412
Psp124BI GAGCTC 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 405
PstI CTGCAG 1 cut(s) 166
PsuI RGATCY 2 cut(s) 241, 403
RsaI GTAC 1 cut(s) 433
RsaNI GTAC 1 cut(s) 432
RseI CAYNNNNRTG 1 cut(s) 319
SacI GAGCTC 1 cut(s) 339
SatI GCNGC 2 cut(s) 165, 522
Sau3AI GATC 2 cut(s) 241, 403
ScaI AGTACT 1 cut(s) 433
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 1 cut(s) 535
SduI GDGCHC 2 cut(s) 339, 495
SfaNI GCATC 1 cut(s) 433
SfcI CTRYAG 1 cut(s) 162
SfuI TTCGAA 2 cut(s) 90, 257
SmiMI CAYNNNNRTG 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
SpeI ACTAGT 1 cut(s) 428
Sse9I AATT 5 cut(s) 41, 264, 345, 387, 526
SspMI CTAG 1 cut(s) 429
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 533
TaaI ACNGT 1 cut(s) 22
TaqI TCGA 4 cut(s) 50, 90, 257, 275
TasI AATT 5 cut(s) 41, 264, 345, 387, 526
TatI WGTACW 1 cut(s) 431
TfiI GAWTC 1 cut(s) 279
TseI GCWGC 2 cut(s) 164, 521
TspDTI ATGAA 3 cut(s) 24, 446, 474
Van91I CCANNNNNTGG 1 cut(s) 376
XapI RAATTY 3 cut(s) 41, 345, 526
XceI RCATGY 1 cut(s) 416
XspI CTAG 1 cut(s) 429
ZrmI AGTACT 1 cut(s) 433
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.