Rroxscaffold_4G00321450

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
N/A
Physical Location & Seq
Reverse (-)
50954810 .. 50985756
30947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00321450.1

Sequence Viewer

Length: 1452 bp
ATGTACGGCATTATTGAAGAATTCTTTCAAAGCCAGAACAACCTCATGCCAATAAGGTACTCCTACTCCAATGTCAAGAAAATGACCAAAGGATTTAAGGATAAATTGGGGGAAGGAGGTTACGGCTCCGTGTATAAAGCAAAGCTTAGGAGTGGTCGCCTTGTTGCTGTTAAGATGTTGGGAAAGTCCAAAGATAATGGGCTAGATTTTATGAATGAAGTTGCTACCATTGGAAGAATTAATCATCCGCTGGAATATTCAAGAAGATTTGGTTTTGGACTTTTCGGGTTACTCGTACAAAAAGACTTACAGAACATTGAGAATCTCGGCTACTATCTCGTCTCTAACCTCGAATTACGGACCGAGCACCATGAGCTCGCTGCTTCTAGTCTTTCCAAGCAACAAGAAGGTTTTGTGAAATCTGCTTCTAATATAAGATATGCACAGGAAGCTGGAAACAATGAAAATGGACGAGATGCTGCTTATATCTTTTATTACATTATAATTTTTGCCTCGATTTCTTCTGGAGAGTTGTTAAGAACATCAAGACGAACTCACTTGGTTCAAGAATCACTCAAAACGAAGCTCATAGCTCGAGATATCTTGATCGAACCAAAACGAGCTCAAAACGGAATCGTGTCCTCCACCGCCGCCGCCGCCGTGTTGTGGTGCAAGGTAGCCACCGGAAGCGTGCAAGGGCGAGGCGAGAGCGTGGGATGTGGTCGGTGCCTTGAGGTGGCACGAGGTGGGAGAGATGAGTTTTTTTGGTGCGGTTTGCTTCGTTTGGTTATGGTTTCGGCAATGAGAGAGAGGAAGAGAGAGAGCGATTGCTCTTGGTTACTATTAGACTCCACCAACTCTACTTACATAAAAACAAAAGCCTCGATTTCTTCTGGAGAGTTGTTAAGAACATCAAGACGAACTCACTGGTTCAAGAATCACTCAAAACGAAGCTCTATAGCTCGAGATATCTTGATCGAACCAAAACGAGCTCAAAACGGAATCGTGTCCTCCACCGCCGCCGCCGCCGTGTTGTGGTGCAAGGTAGCCACCGGAAGCTGTGCAAGGGCGAGGCGAGAGCGTGGGATGTGGTCTGGTGCCTTGAGGTGGCCTGAGTATCCATCCCTAAGAAGTATACTTCAGGAGAAATCCATGGAGTACTTGTTACATCGATCGATTATGATACTCACCATGTTGAATGCGGAGATTCTGGTATACACGCACATATTCTTACTGTTGATATATCAAGTGTCTAACATTAATATGTCAACCGATATTATTGTTAAGATGTACTTCAGCTTATTGTTGTCTTCGAACAATGCAAACATCATGGATCGCCTCTGGGATAACATGATTAGCCGGTATATAATATATTGTGAGGGCTCTGTCAATCTTATTGTATTCATTCTTTCCGATCCTTTTCCCAACATCTTAATCACCTCTCAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

483

Amino Acids

54.6

Weight (kDa)

9.61

Isoelectric Point (pI)

53.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 503
AccB1I GGYRCC 2 cut(s) 726, 1097
AccI GTMKAC 2 cut(s) 1135, 1215
AclWI GGATC 2 cut(s) 1341, 1409
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 2 cut(s) 1124, 1279
AdeI CACNNNGTG 1 cut(s) 746
AfaI GTAC 5 cut(s) 5, 59, 297, 1160, 1292
AfiI CCNNNNNNNGG 4 cut(s) 666, 736, 1035, 1107
AgsI TTSAA 6 cut(s) 17, 29, 261, 566, 934, 1198
Alw21I GWGCWC 4 cut(s) 369, 378, 625, 994
Alw26I GTCTC 1 cut(s) 346
AlwI GGATC 2 cut(s) 1341, 1409
Ama87I CYCGRG 2 cut(s) 594, 963
AoxI GGCC 1 cut(s) 1109
ApeKI GCWGC 2 cut(s) 380, 479
ApoI RAATTY 1 cut(s) 20
AseI ATTAAT 2 cut(s) 240, 1260
Asp700I GAANNNNTTC 1 cut(s) 24
AspS9I GGNCC 1 cut(s) 360
AsuHPI GGTGA 2 cut(s) 1180, 1429
AsuII TTCGAA 1 cut(s) 1313
AvaI CYCGRG 2 cut(s) 594, 963
AvaII GGWCC 1 cut(s) 360
BanI GGYRCC 2 cut(s) 726, 1097
BanII GRGCYC 4 cut(s) 378, 625, 994, 1385
BauI CACGAG 1 cut(s) 741
BbsI GAAGAC 1 cut(s) 1302
Bbv12I GWGCWC 4 cut(s) 369, 378, 625, 994
BbvI GCAGC 2 cut(s) 367, 466
BccI CCATC 1 cut(s) 1129
BceAI ACGGC 4 cut(s) 22, 139, 644, 1013
BciVI GTATCC 1 cut(s) 1128
BcoDI GTCTC 1 cut(s) 346
BfaI CTAG 2 cut(s) 203, 387
BfmI CTRYAG 1 cut(s) 957
BfuI GTATCC 1 cut(s) 1128
BisI GCNGC 8 cut(s) 381, 480, 651, 654, 657, 1020, 1023, 1026
BlsI GCNGC 8 cut(s) 382, 481, 652, 655, 658, 1021, 1024, 1027
BmcAI AGTACT 1 cut(s) 1160
Bme18I GGWCC 1 cut(s) 360
BmeT110I CYCGRG 2 cut(s) 594, 963
BmgT120I GGNCC 1 cut(s) 360
BmiI GGNNCC 3 cut(s) 127, 728, 1099
BmsI GCATC 1 cut(s) 466
BpiI GAAGAC 1 cut(s) 1302
BpmI CTGGAG 2 cut(s) 546, 915
Bpu10I CCTNAGC 1 cut(s) 146
Bpu14I TTCGAA 1 cut(s) 1313
BpuEI CTTGAG 2 cut(s) 752, 1123
Bsa29I ATCGAT 2 cut(s) 1171, 1175
BsaJI CCNNGG 1 cut(s) 1152
BsaWI WCCGGW 2 cut(s) 683, 1052
BsaXI ACNNNNNCTCC 4 cut(s) 50, 80, 1148, 1178
Bsc4I CCNNNNNNNGG 4 cut(s) 666, 736, 1035, 1107
Bse118I RCCGGY 1 cut(s) 1359
Bse1I ACTGG 1 cut(s) 932
Bse3DI GCAATG 1 cut(s) 807
BseCI ATCGAT 2 cut(s) 1171, 1175
BseDI CCNNGG 1 cut(s) 1152
BseGI GGATG 4 cut(s) 244, 722, 1092, 1121
BseLI CCNNNNNNNGG 4 cut(s) 666, 736, 1035, 1107
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 1 cut(s) 1104
BseNI ACTGG 1 cut(s) 932
BseXI GCAGC 2 cut(s) 367, 466
Bsh1285I CGRYCG 1 cut(s) 1175
BshFI GGCC 1 cut(s) 1111
BshNI GGYRCC 2 cut(s) 726, 1097
BshVI ATCGAT 2 cut(s) 1171, 1175
BsiEI CGRYCG 1 cut(s) 1175
BsiHKAI GWGCWC 4 cut(s) 369, 378, 625, 994
BsiHKCI CYCGRG 2 cut(s) 594, 963
BsiSI CCGG 3 cut(s) 684, 1053, 1360
BslI CCNNNNNNNGG 4 cut(s) 666, 736, 1035, 1107
BsmAI GTCTC 1 cut(s) 346
BsmBI CGTCTC 1 cut(s) 346
BsmI GAATGC 1 cut(s) 1204
BsnI GGCC 1 cut(s) 1111
BsoBI CYCGRG 2 cut(s) 594, 963
Bsp119I TTCGAA 1 cut(s) 1313
Bsp1286I GDGCHC 5 cut(s) 369, 378, 625, 994, 1385
Bsp143I GATC 5 cut(s) 606, 975, 1172, 1333, 1414
Bsp19I CCATGG 1 cut(s) 1152
BspANI GGCC 1 cut(s) 1111
BspCNI CTCAG 1 cut(s) 1105
BspDI ATCGAT 2 cut(s) 1171, 1175
BspLI GGNNCC 3 cut(s) 127, 728, 1099
BspPI GGATC 2 cut(s) 1341, 1409
BspT104I TTCGAA 1 cut(s) 1313
BspT107I GGYRCC 2 cut(s) 726, 1097
BsrDI GCAATG 1 cut(s) 807
BsrFI RCCGGY 1 cut(s) 1359
BsrI ACTGG 1 cut(s) 932
BssAI RCCGGY 1 cut(s) 1359
BssECI CCNNGG 1 cut(s) 1152
BssMI GATC 5 cut(s) 606, 975, 1172, 1333, 1414
BssNAI GTATAC 2 cut(s) 1136, 1216
BssSI CACGAG 1 cut(s) 741
BssT1I CCWWGG 1 cut(s) 1152
Bst1107I GTATAC 2 cut(s) 1136, 1216
Bst2BI CACGAG 1 cut(s) 741
Bst4CI ACNGT 1 cut(s) 1236
Bst6I CTCTTC 1 cut(s) 809
BstBI TTCGAA 1 cut(s) 1313
BstC8I GCNNGC 2 cut(s) 378, 692
BstDEI CTNAG 4 cut(s) 146, 1113, 1127, 1443
BstDSI CCRYGG 1 cut(s) 1152
BstF5I GGATG 4 cut(s) 244, 722, 1092, 1121
BstKTI GATC 5 cut(s) 609, 978, 1175, 1336, 1417
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 5 cut(s) 606, 975, 1172, 1333, 1414
BstMCI CGRYCG 1 cut(s) 1175
BstMWI GCNNNNNNNGC 4 cut(s) 373, 449, 656, 1025
BstSFI CTRYAG 1 cut(s) 957
BstV1I GCAGC 2 cut(s) 367, 466
BstV2I GAAGAC 1 cut(s) 1302
BstZ17I GTATAC 2 cut(s) 1136, 1216
Bsu15I ATCGAT 2 cut(s) 1171, 1175
BsuI GTATCC 1 cut(s) 1128
BsuRI GGCC 1 cut(s) 1111
BsuTUI ATCGAT 2 cut(s) 1171, 1175
BtgI CCRYGG 1 cut(s) 1152
BtsCI GGATG 4 cut(s) 244, 722, 1092, 1121
BtsIMutI CAGTG 1 cut(s) 925
Cac8I GCNNGC 2 cut(s) 378, 692
Cfr10I RCCGGY 1 cut(s) 1359
Cfr13I GGNCC 1 cut(s) 360
ClaI ATCGAT 2 cut(s) 1171, 1175
CpoI CGGWCCG 1 cut(s) 360
Csp6I GTAC 5 cut(s) 4, 58, 296, 1159, 1291
CspI CGGWCCG 1 cut(s) 360
CviAII CATG 6 cut(s) 46, 371, 1153, 1192, 1330, 1351
CviQI GTAC 5 cut(s) 4, 58, 296, 1159, 1291
DdeI CTNAG 4 cut(s) 146, 1113, 1127, 1443
DpnI GATC 5 cut(s) 608, 977, 1174, 1335, 1416
DpnII GATC 5 cut(s) 606, 975, 1172, 1333, 1414
DraIII CACNNNGTG 1 cut(s) 746
Eam1104I CTCTTC 1 cut(s) 809
EarI CTCTTC 1 cut(s) 809
Ecl136II GAGCTC 3 cut(s) 376, 623, 992
Eco130I CCWWGG 1 cut(s) 1152
Eco24I GRGCYC 4 cut(s) 378, 625, 994, 1385
Eco32I GATATC 2 cut(s) 601, 970
Eco47I GGWCC 1 cut(s) 360
Eco53kI GAGCTC 3 cut(s) 376, 623, 992
Eco57I CTGAAG 2 cut(s) 1124, 1279
Eco88I CYCGRG 2 cut(s) 594, 963
EcoICRI GAGCTC 3 cut(s) 376, 623, 992
EcoRI GAATTC 1 cut(s) 20
EcoRV GATATC 2 cut(s) 601, 970
EcoT14I CCWWGG 1 cut(s) 1152
EcoT38I GRGCYC 4 cut(s) 378, 625, 994, 1385
ErhI CCWWGG 1 cut(s) 1152
Esp3I CGTCTC 1 cut(s) 346
FaeI CATG 6 cut(s) 49, 374, 1156, 1195, 1333, 1354
FalI AAGNNNNNCTT 4 cut(s) 129, 161, 1277, 1309
FatI CATG 6 cut(s) 45, 370, 1152, 1191, 1329, 1350
FblI GTMKAC 2 cut(s) 1135, 1215
Fnu4HI GCNGC 8 cut(s) 381, 480, 651, 654, 657, 1020, 1023, 1026
FokI GGATG 4 cut(s) 231, 729, 1099, 1108
FriOI GRGCYC 4 cut(s) 378, 625, 994, 1385
Fsp4HI GCNGC 8 cut(s) 381, 480, 651, 654, 657, 1020, 1023, 1026
FspBI CTAG 2 cut(s) 203, 387
GluI GCNGC 8 cut(s) 381, 480, 651, 654, 657, 1020, 1023, 1026
GsuI CTGGAG 2 cut(s) 546, 915
HaeIII GGCC 1 cut(s) 1111
HapII CCGG 3 cut(s) 684, 1053, 1360
Hin1II CATG 6 cut(s) 49, 374, 1156, 1195, 1333, 1354
HincII GTYRAC 1 cut(s) 1269
HindII GTYRAC 1 cut(s) 1269
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 7 cut(s) 322, 569, 633, 848, 937, 1002, 1207
HpaII CCGG 3 cut(s) 684, 1053, 1360
HphI GGTGA 2 cut(s) 1180, 1429
Hpy166II GTNNAC 3 cut(s) 1136, 1216, 1269
Hpy188I TCNGA 1 cut(s) 1414
Hpy8I GTNNAC 3 cut(s) 1136, 1216, 1269
HpyAV CCTTC 2 cut(s) 107, 401
HpyCH4III ACNGT 1 cut(s) 1236
HpyCH4V TGCA 6 cut(s) 443, 672, 694, 1041, 1064, 1322
HpyF10VI GCNNNNNNNGC 4 cut(s) 373, 449, 656, 1025
HpyF3I CTNAG 4 cut(s) 146, 1113, 1127, 1443
Hsp92II CATG 6 cut(s) 49, 374, 1156, 1195, 1333, 1354
Kzo9I GATC 5 cut(s) 606, 975, 1172, 1333, 1414
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 2 cut(s) 367, 466
LweI GCATC 1 cut(s) 466
MaeI CTAG 2 cut(s) 203, 387
MaeIII GTNAC 4 cut(s) 119, 288, 837, 1164
MalI GATC 5 cut(s) 608, 977, 1174, 1335, 1416
MboI GATC 5 cut(s) 606, 975, 1172, 1333, 1414
MboII GAAGA 7 cut(s) 29, 246, 276, 513, 826, 882, 1302
MhlI GDGCHC 5 cut(s) 369, 378, 625, 994, 1385
MluCI AATT 5 cut(s) 20, 104, 237, 353, 504
MlyI GAGTC 1 cut(s) 842
MroXI GAANNNNTTC 1 cut(s) 24
MseI TTAA 9 cut(s) 96, 171, 240, 536, 905, 1260, 1284, 1433, 1450
MslI CAYNNNNRTG 1 cut(s) 1262
MspA1I CMGCKG 1 cut(s) 250
MspI CCGG 3 cut(s) 684, 1053, 1360
Mva1269I GAATGC 1 cut(s) 1204
MwoI GCNNNNNNNGC 4 cut(s) 373, 449, 656, 1025
NcoI CCATGG 1 cut(s) 1152
NdeII GATC 5 cut(s) 606, 975, 1172, 1333, 1414
NlaIII CATG 6 cut(s) 49, 374, 1156, 1195, 1333, 1354
NlaIV GGNNCC 3 cut(s) 127, 728, 1099
NmeAIII GCCGAG 1 cut(s) 306
NspV TTCGAA 1 cut(s) 1313
PaeR7I CTCGAG 2 cut(s) 594, 963
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 1 cut(s) 1204
PdmI GAANNNNTTC 1 cut(s) 24
PfeI GAWTC 6 cut(s) 322, 569, 633, 937, 1002, 1207
PkrI GCNGC 8 cut(s) 382, 481, 652, 655, 658, 1021, 1024, 1027
Ple19I CGATCG 1 cut(s) 1175
PleI GAGTC 1 cut(s) 842
PpsI GAGTC 1 cut(s) 842
PshBI ATTAAT 2 cut(s) 240, 1260
PsiI TTATAA 1 cut(s) 503
Psp124BI GAGCTC 3 cut(s) 378, 625, 994
PspN4I GGNNCC 3 cut(s) 127, 728, 1099
PspPI GGNCC 1 cut(s) 360
PvuI CGATCG 1 cut(s) 1175
RsaI GTAC 5 cut(s) 5, 59, 297, 1160, 1292
RsaNI GTAC 5 cut(s) 4, 58, 296, 1159, 1291
RseI CAYNNNNRTG 1 cut(s) 1262
Rsr2I CGGWCCG 1 cut(s) 360
RsrII CGGWCCG 1 cut(s) 360
SacI GAGCTC 3 cut(s) 378, 625, 994
SaqAI TTAA 9 cut(s) 96, 171, 240, 536, 905, 1260, 1284, 1433, 1450
SatI GCNGC 8 cut(s) 381, 480, 651, 654, 657, 1020, 1023, 1026
Sau3AI GATC 5 cut(s) 606, 975, 1172, 1333, 1414
Sau96I GGNCC 1 cut(s) 360
ScaI AGTACT 1 cut(s) 1160
SchI GAGTC 1 cut(s) 842
SduI GDGCHC 5 cut(s) 369, 378, 625, 994, 1385
SfaNI GCATC 1 cut(s) 466
SfcI CTRYAG 1 cut(s) 957
Sfr274I CTCGAG 2 cut(s) 594, 963
SfuI TTCGAA 1 cut(s) 1313
SinI GGWCC 1 cut(s) 360
SlaI CTCGAG 2 cut(s) 594, 963
SmiMI CAYNNNNRTG 1 cut(s) 1262
SmlI CTYRAG 4 cut(s) 594, 731, 963, 1102
SmoI CTYRAG 4 cut(s) 594, 731, 963, 1102
Sse9I AATT 5 cut(s) 20, 104, 237, 353, 504
SspI AATATT 1 cut(s) 257
SspMI CTAG 2 cut(s) 203, 387
SstI GAGCTC 3 cut(s) 378, 625, 994
StyI CCWWGG 1 cut(s) 1152
TaaI ACNGT 1 cut(s) 1236
TaqII GACCGA 1 cut(s) 377
TasI AATT 5 cut(s) 20, 104, 237, 353, 504
TatI WGTACW 2 cut(s) 1158, 1290
TauI GCSGC 6 cut(s) 653, 656, 659, 1022, 1025, 1028
TfiI GAWTC 6 cut(s) 322, 569, 633, 937, 1002, 1207
Tru1I TTAA 9 cut(s) 96, 171, 240, 536, 905, 1260, 1284, 1433, 1450
Tru9I TTAA 9 cut(s) 96, 171, 240, 536, 905, 1260, 1284, 1433, 1450
TscAI CASTG 1 cut(s) 932
TseI GCWGC 2 cut(s) 380, 479
TspDTI ATGAA 4 cut(s) 227, 231, 477, 1393
TspGWI ACGGA 4 cut(s) 118, 373, 645, 1014
TspRI CASTG 1 cut(s) 932
VpaK11BI GGWCC 1 cut(s) 360
VspI ATTAAT 2 cut(s) 240, 1260
XapI RAATTY 1 cut(s) 20
XhoI CTCGAG 2 cut(s) 594, 963
XmiI GTMKAC 2 cut(s) 1135, 1215
XmnI GAANNNNTTC 1 cut(s) 24
XspI CTAG 2 cut(s) 203, 387
ZrmI AGTACT 1 cut(s) 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.