Rroxscaffold_4G00325380

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
N/A
Physical Location & Seq
Reverse (-)
57082512 .. 57083162
651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325380.1

Sequence Viewer

Length: 564 bp
ATGGAGAAAGGAACAACATTATTGGAAGAGACTAACAAGCAAAGAGGACGAATTAGTTTTCACTTTAACCCTTTGGATGGAAGCCCTATAAAATATAACGTAGGCACACTTTTTTTTCTCGTCGAATACAATACACACTGTCCAACGGGAAATTATAGCAGTGGTAATGTGTGCAGTGGCCTTATTACCAGGCGACCAGTACTCCACACCCCCGTCACCAAAGTTCTCCAACAGTACATTCACCTTTCCCATGTCCAGCAACAGCACGCTCGAGCTGCCACCTCCATACACCATTTCTCTCACAAGAACAACTTCTTCGCATTGGGGTGTAACAGTGTAGCAGTGTATCAAGGTTACCAAAGCAAAGTCCCAGATCCAGAAGAAAGCTACAAACGCGGGTACACCGTGAGCCTGTGTCAAGATGCTCTGGCACAGCTCTTCCCATCAATTCTTGCAATGGTTTCGGGTGTAGCCAAAATGCGATCCCTAGTAGACTGCAAAACATGTCGGTGGAATTGTATAAACTTGATCGCATCATCAGAAATTGGAACCACTGGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

187

Amino Acids

20.78

Weight (kDa)

9.0

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 492
AccII CGCG 1 cut(s) 396
AciI CCGC 1 cut(s) 396
AclWI GGATC 2 cut(s) 368, 477
AfaI GTAC 3 cut(s) 201, 236, 401
AfiI CCNNNNNNNGG 1 cut(s) 77
AflIII ACRYGT 1 cut(s) 503
AjnI CCWGG 1 cut(s) 188
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AluBI AGCT 3 cut(s) 275, 387, 436
AluI AGCT 3 cut(s) 275, 387, 436
Alw26I GTCTC 1 cut(s) 23
AlwI GGATC 2 cut(s) 368, 477
Ama87I CYCGRG 1 cut(s) 270
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 1 cut(s) 275
Asp700I GAANNNNTTC 1 cut(s) 311
AsuHPI GGTGA 2 cut(s) 208, 233
AvaI CYCGRG 1 cut(s) 270
BbvI GCAGC 1 cut(s) 262
BccI CCATC 2 cut(s) 71, 451
BcgI CGANNNNNNTGC 2 cut(s) 444, 478
BciT130I CCWGG 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 1 cut(s) 488
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
BmcAI AGTACT 1 cut(s) 201
Bme1390I CCNGG 1 cut(s) 190
BmeT110I CYCGRG 1 cut(s) 270
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 2 cut(s) 412, 542
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 197, 559
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 1 cut(s) 190
BseGI GGATG 1 cut(s) 82
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMI GCAATG 1 cut(s) 462
BseNI ACTGG 2 cut(s) 197, 559
BseXI GCAGC 1 cut(s) 262
BsgI GTGCAG 1 cut(s) 193
Bsh1236I CGCG 1 cut(s) 396
BshFI GGCC 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 270
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 353
BsnI GGCC 1 cut(s) 180
BsoBI CYCGRG 1 cut(s) 270
Bsp143I GATC 3 cut(s) 373, 482, 528
BspACI CCGC 1 cut(s) 396
BspANI GGCC 1 cut(s) 180
BspFNI CGCG 1 cut(s) 396
BspLI GGNNCC 1 cut(s) 550
BspPI GGATC 2 cut(s) 368, 477
BspQI GCTCTTC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 462
BsrI ACTGG 2 cut(s) 197, 559
BssMI GATC 3 cut(s) 373, 482, 528
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 4 cut(s) 140, 234, 335, 406
Bst6I CTCTTC 2 cut(s) 21, 443
BstC8I GCNNGC 1 cut(s) 267
BstEII GGTNACC 1 cut(s) 353
BstF5I GGATG 1 cut(s) 82
BstFNI CGCG 1 cut(s) 396
BstKTI GATC 3 cut(s) 376, 485, 531
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 3 cut(s) 373, 482, 528
BstMWI GCNNNNNNNGC 2 cut(s) 275, 393
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 507
BstPI GGTNACC 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 188
BstUI CGCG 1 cut(s) 396
BstV1I GCAGC 1 cut(s) 262
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BsuRI GGCC 1 cut(s) 180
BtsCI GGATG 1 cut(s) 82
BtsI GCAGTG 3 cut(s) 166, 181, 348
BtsIMutI CAGTG 6 cut(s) 136, 166, 181, 340, 348, 552
Cac8I GCNNGC 1 cut(s) 267
Csp6I GTAC 3 cut(s) 200, 235, 400
CviAII CATG 2 cut(s) 251, 504
CviJI RGCY 7 cut(s) 84, 180, 275, 387, 411, 436, 473
CviKI_1 RGCY 7 cut(s) 84, 180, 275, 387, 411, 436, 473
CviQI GTAC 3 cut(s) 200, 235, 400
DpnI GATC 3 cut(s) 375, 484, 530
DpnII GATC 3 cut(s) 373, 482, 528
Eam1104I CTCTTC 2 cut(s) 21, 443
EarI CTCTTC 2 cut(s) 21, 443
Eco88I CYCGRG 1 cut(s) 270
Eco91I GGTNACC 1 cut(s) 353
EcoO65I GGTNACC 1 cut(s) 353
EcoRII CCWGG 1 cut(s) 188
FaeI CATG 2 cut(s) 254, 507
FaiI YATR 8 cut(s) 89, 96, 156, 252, 287, 505, 521, 562
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 1 cut(s) 353
FatI CATG 2 cut(s) 250, 503
FauI CCCGC 1 cut(s) 389
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 1 cut(s) 488
GluI GCNGC 1 cut(s) 276
HaeIII GGCC 1 cut(s) 180
Hin1II CATG 2 cut(s) 254, 507
HphI GGTGA 2 cut(s) 208, 233
Hpy166II GTNNAC 2 cut(s) 402, 493
Hpy188I TCNGA 1 cut(s) 541
Hpy188III TCNNGA 2 cut(s) 377, 419
Hpy8I GTNNAC 2 cut(s) 402, 493
Hpy99I CGWCG 1 cut(s) 125
HpyCH4III ACNGT 4 cut(s) 140, 234, 335, 406
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 3 cut(s) 174, 455, 498
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 393
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 2 cut(s) 254, 507
Kzo9I GATC 3 cut(s) 373, 482, 528
LguI GCTCTTC 1 cut(s) 443
LpnPI CCDG 9 cut(s) 175, 202, 210, 269, 384, 390, 413, 425, 540
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 2 cut(s) 412, 542
MaeI CTAG 1 cut(s) 488
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 3 cut(s) 214, 329, 353
MalI GATC 3 cut(s) 375, 484, 530
MboI GATC 3 cut(s) 373, 482, 528
MboII GAAGA 4 cut(s) 38, 307, 392, 430
MflI RGATCY 1 cut(s) 373
MluCI AATT 5 cut(s) 51, 151, 447, 514, 543
MmeI TCCRAC 2 cut(s) 167, 253
MnlI CCTC 2 cut(s) 38, 292
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 1 cut(s) 66
MslI CAYNNNNRTG 2 cut(s) 325, 508
MspR9I CCNGG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 1 cut(s) 396
MwoI GCNNNNNNNGC 2 cut(s) 275, 393
NdeII GATC 3 cut(s) 373, 482, 528
NlaIII CATG 2 cut(s) 254, 507
NlaIV GGNNCC 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 214
NspI RCATGY 1 cut(s) 507
PaeR7I CTCGAG 1 cut(s) 270
PciI ACATGT 1 cut(s) 503
PciSI GCTCTTC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 311
PkrI GCNGC 1 cut(s) 277
PscI ACATGT 1 cut(s) 503
Psp6I CCWGG 1 cut(s) 188
PspEI GGTNACC 1 cut(s) 353
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 1 cut(s) 550
PspXI VCTCGAGB 1 cut(s) 270
PsuI RGATCY 1 cut(s) 373
RsaI GTAC 3 cut(s) 201, 236, 401
RsaNI GTAC 3 cut(s) 200, 235, 400
RseI CAYNNNNRTG 2 cut(s) 325, 508
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 3 cut(s) 373, 482, 528
ScaI AGTACT 1 cut(s) 201
ScrFI CCNGG 1 cut(s) 190
SetI ASST 7 cut(s) 102, 246, 277, 284, 355, 389, 438
SfaNI GCATC 2 cut(s) 412, 542
Sfr274I CTCGAG 1 cut(s) 270
SlaI CTCGAG 1 cut(s) 270
SmiMI CAYNNNNRTG 2 cut(s) 325, 508
SmlI CTYRAG 1 cut(s) 270
SmoI CTYRAG 1 cut(s) 270
Sse9I AATT 5 cut(s) 51, 151, 447, 514, 543
SsiI CCGC 1 cut(s) 396
SspMI CTAG 1 cut(s) 488
StyD4I CCNGG 1 cut(s) 188
TaaI ACNGT 4 cut(s) 140, 234, 335, 406
TaiI ACGT 1 cut(s) 102
TaqI TCGA 2 cut(s) 123, 271
TasI AATT 5 cut(s) 51, 151, 447, 514, 543
TatI WGTACW 2 cut(s) 199, 234
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TscAI CASTG 6 cut(s) 143, 166, 181, 340, 348, 559
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 1 cut(s) 214
TspRI CASTG 6 cut(s) 143, 166, 181, 340, 348, 559
XceI RCATGY 1 cut(s) 507
XhoI CTCGAG 1 cut(s) 270
XmiI GTMKAC 1 cut(s) 492
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 1 cut(s) 488
ZrmI AGTACT 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.