Rroxscaffold_5G00335360

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
N/A
Physical Location & Seq
Forward (+)
2753964 .. 2754352
389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00335360.1

Sequence Viewer

Length: 273 bp
ATGAACTGGTCAAGATACTTGACTCGCGAGAAAATTCAAAGGCCGGAGTTTAGAGCTGTTTTCTACAAGGTGGCTCCCTCGGATGTACGACACCAAACAGGTGCTTTCGGTGATGCATTTGCTAAAATTGATCAATGCGTATACAAGGATAGCATCGGCAAATGGAAGGCAGCTCTCAAGGAAGCAGCAGATTTGTCCGGATGGACTTTCGAGGAGGGCGGTACGAGACTGAATTTATCAATGGCATTGTTGGGGAGTTGTCTACCCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

90

Amino Acids

10.17

Weight (kDa)

8.64

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 71 1.4e-10 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 141, 262
AccII CGCG 1 cut(s) 27
AccIII TCCGGA 1 cut(s) 197
AciI CCGC 1 cut(s) 219
AcsI RAATTY 2 cut(s) 33, 232
AfaI GTAC 2 cut(s) 87, 223
AgsI TTSAA 1 cut(s) 38
AluBI AGCT 2 cut(s) 56, 173
AluI AGCT 2 cut(s) 56, 173
Alw26I GTCTC 1 cut(s) 220
Ama87I CYCGRG 1 cut(s) 266
Aor13HI TCCGGA 1 cut(s) 197
AoxI GGCC 1 cut(s) 41
ApeKI GCWGC 2 cut(s) 170, 185
ApoI RAATTY 2 cut(s) 33, 232
AsuHPI GGTGA 1 cut(s) 122
AvaI CYCGRG 1 cut(s) 266
BbvI GCAGC 2 cut(s) 182, 197
BccI CCATC 1 cut(s) 195
BclI TGATCA 1 cut(s) 130
BcoDI GTCTC 1 cut(s) 220
BisI GCNGC 2 cut(s) 171, 186
BlsI GCNGC 2 cut(s) 172, 187
BmeT110I CYCGRG 1 cut(s) 266
BmiI GGNNCC 1 cut(s) 75
BmsI GCATC 2 cut(s) 103, 162
BpuEI CTTGAG 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 78
BsaWI WCCGGW 1 cut(s) 197
Bse1I ACTGG 1 cut(s) 11
BseAI TCCGGA 1 cut(s) 197
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 2 cut(s) 88, 206
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 1 cut(s) 227
BseXI GCAGC 2 cut(s) 182, 197
Bsh1236I CGCG 1 cut(s) 27
BshFI GGCC 1 cut(s) 43
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 2 cut(s) 44, 198
BsmAI GTCTC 1 cut(s) 220
BsnI GGCC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 266
Bsp13I TCCGGA 1 cut(s) 197
Bsp143I GATC 1 cut(s) 130
Bsp68I TCGCGA 1 cut(s) 27
BspACI CCGC 1 cut(s) 219
BspANI GGCC 1 cut(s) 43
BspEI TCCGGA 1 cut(s) 197
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 1 cut(s) 75
BsrI ACTGG 1 cut(s) 11
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 1 cut(s) 130
BssNAI GTATAC 1 cut(s) 142
Bst1107I GTATAC 1 cut(s) 142
BstF5I GGATG 2 cut(s) 88, 206
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 1 cut(s) 133
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 1 cut(s) 130
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 2 cut(s) 182, 197
BstZ17I GTATAC 1 cut(s) 142
BsuRI GGCC 1 cut(s) 43
BtsCI GGATG 2 cut(s) 88, 206
BtuMI TCGCGA 1 cut(s) 27
Csp6I GTAC 2 cut(s) 86, 222
CviJI RGCY 4 cut(s) 43, 56, 74, 173
CviKI_1 RGCY 4 cut(s) 43, 56, 74, 173
CviQI GTAC 2 cut(s) 86, 222
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 266
EcoT22I ATGCAT 1 cut(s) 118
FaiI YATR 1 cut(s) 142
FbaI TGATCA 1 cut(s) 130
FblI GTMKAC 2 cut(s) 141, 262
Fnu4HI GCNGC 2 cut(s) 171, 186
FokI GGATG 2 cut(s) 95, 213
Fsp4HI GCNGC 2 cut(s) 171, 186
GluI GCNGC 2 cut(s) 171, 186
HaeIII GGCC 1 cut(s) 43
HapII CCGG 2 cut(s) 44, 198
HinfI GANTC 1 cut(s) 22
HpaII CCGG 2 cut(s) 44, 198
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 2 cut(s) 142, 263
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 3 cut(s) 12, 26, 198
Hpy8I GTNNAC 2 cut(s) 142, 263
HpyAV CCTTC 1 cut(s) 160
HpyCH4V TGCA 1 cut(s) 116
Kpn2I TCCGGA 1 cut(s) 197
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 1 cut(s) 130
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 3 cut(s) 57, 84, 211
Lsp1109I GCAGC 2 cut(s) 182, 197
LweI GCATC 2 cut(s) 103, 162
MalI GATC 1 cut(s) 132
MboI GATC 1 cut(s) 130
MluCI AATT 3 cut(s) 33, 126, 232
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 3 cut(s) 88, 205, 208
Mph1103I ATGCAT 1 cut(s) 118
MroI TCCGGA 1 cut(s) 197
MspI CCGG 2 cut(s) 44, 198
MvnI CGCG 1 cut(s) 27
NdeII GATC 1 cut(s) 130
NlaIV GGNNCC 1 cut(s) 75
NruI TCGCGA 1 cut(s) 27
NsiI ATGCAT 1 cut(s) 118
PkrI GCNGC 2 cut(s) 172, 187
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 75
RruI TCGCGA 1 cut(s) 27
RsaI GTAC 2 cut(s) 87, 223
RsaNI GTAC 2 cut(s) 86, 222
SatI GCNGC 2 cut(s) 171, 186
Sau3AI GATC 1 cut(s) 130
SchI GAGTC 1 cut(s) 16
SetI ASST 4 cut(s) 58, 72, 103, 175
SfaNI GCATC 2 cut(s) 103, 162
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 3 cut(s) 33, 126, 232
SsiI CCGC 1 cut(s) 219
TaqI TCGA 1 cut(s) 210
TasI AATT 3 cut(s) 33, 126, 232
TseI GCWGC 2 cut(s) 170, 185
TspDTI ATGAA 1 cut(s) 17
XapI RAATTY 2 cut(s) 33, 232
XmiI GTMKAC 2 cut(s) 141, 262
Zsp2I ATGCAT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.