Rroxscaffold_5G00342650

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
N/A
Physical Location & Seq
Reverse (-)
11769811 .. 11772867
3057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00342650.1

Sequence Viewer

Length: 636 bp
ATGGCAGGTGGTGTTGTTCAAAATTCTGCCATACGAGAAGTAGCAACGGAAGTAGCCAAAAAGTGCGGAGGCTTGCCGGTTTTAGTGGTAACGATTGCAAGCGCCCTGAAGAATAGAAGTACATTACATGCTTGGAAAGATGTCTTGCGACGCCTAAAAACATTTGACAAACAAGGATTTACGGAAAAAGTATTCTTGGCTCTAGAGTGGAGTTACGAGCAATTAGATGATGAGGAGCTTAAGCCATTAGATTCCAAATATGTATTGGTTCTGCGTGGAAATGGGAAAGCTGTGGATGAAGCCCTCAATGCACTAAAGCTCCGACTCACCGCTAGCAATGAATTGGACCAAGGTCTAAGAATGTTGCTGAAAGGAACTGAAGACTTGTACTTGGAGGGACGGAGGGTTAGGTATCCAAACTTAACATGGTTGGCGGTGAATGAACTCAATGGTTTAAGATTCTTGTTATCAGCTCCAATGGCTAGAAGCCTTACACAACTCGAACGTCTTCAAGTATCGGGATGTCAAATAATGGAAGAGATAGTATCAATAGAAGAATATGATGAAGAAATTGTAGACAACTTCTTTTTGCCAGCTACAAATTCTGGAGCTAAAAGACCTTCCAAATCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

211

Amino Acids

23.68

Weight (kDa)

6.21

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 138 - 189 3.4e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 576
AciI CCGC 3 cut(s) 66, 330, 434
AcsI RAATTY 2 cut(s) 22, 601
AcuI CTGAAG 2 cut(s) 128, 399
AcyI GRCGYC 1 cut(s) 151
AfaI GTAC 2 cut(s) 121, 389
AflII CTTAAG 1 cut(s) 239
AgsI TTSAA 2 cut(s) 20, 512
AluBI AGCT 6 cut(s) 238, 290, 319, 473, 596, 611
AluI AGCT 6 cut(s) 238, 290, 319, 473, 596, 611
ApoI RAATTY 2 cut(s) 22, 601
Asp700I GAANNNNTTC 1 cut(s) 507
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 1 cut(s) 346
AsuHPI GGTGA 2 cut(s) 319, 448
AsuNHI GCTAGC 1 cut(s) 332
AvaII GGWCC 1 cut(s) 346
BbsI GAAGAC 2 cut(s) 387, 500
BciVI GTATCC 1 cut(s) 423
BfaI CTAG 3 cut(s) 203, 333, 483
BfoI RGCGCY 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 239
BfuI GTATCC 1 cut(s) 423
Bme18I GGWCC 1 cut(s) 346
BmgT120I GGNCC 1 cut(s) 346
BmtI GCTAGC 1 cut(s) 336
BoxI GACNNNNGTC 1 cut(s) 351
BpiI GAAGAC 2 cut(s) 387, 500
BpmI CTGGAG 1 cut(s) 627
BsaHI GRCGYC 1 cut(s) 151
BsaJI CCNNGG 1 cut(s) 349
BsaXI ACNNNNNCTCC 2 cut(s) 303, 333
Bse118I RCCGGY 1 cut(s) 76
Bse3DI GCAATG 1 cut(s) 343
BseDI CCNNGG 1 cut(s) 349
BseGI GGATG 2 cut(s) 301, 527
BseMI GCAATG 1 cut(s) 343
BseRI GAGGAG 1 cut(s) 248
BsiSI CCGG 1 cut(s) 77
BslFI GGGAC 1 cut(s) 411
BsmFI GGGAC 1 cut(s) 411
BspACI CCGC 3 cut(s) 66, 330, 434
BspOI GCTAGC 1 cut(s) 336
BspTI CTTAAG 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 76
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 349
BssNI GRCGYC 1 cut(s) 151
BssT1I CCWWGG 1 cut(s) 349
Bst6I CTCTTC 1 cut(s) 531
BstACI GRCGYC 1 cut(s) 151
BstAFI CTTAAG 1 cut(s) 239
BstC8I GCNNGC 4 cut(s) 74, 100, 334, 594
BstDEI CTNAG 1 cut(s) 356
BstF5I GGATG 2 cut(s) 301, 527
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 1 cut(s) 104
BstMWI GCNNNNNNNGC 2 cut(s) 308, 479
BstNSI RCATGY 1 cut(s) 131
BstPAI GACNNNNGTC 1 cut(s) 351
BstV2I GAAGAC 2 cut(s) 387, 500
BsuI GTATCC 1 cut(s) 423
BtsCI GGATG 2 cut(s) 301, 527
Cac8I GCNNGC 4 cut(s) 74, 100, 334, 594
CfoI GCGC 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 346
CseI GACGC 1 cut(s) 159
Csp6I GTAC 2 cut(s) 120, 388
CviAII CATG 2 cut(s) 128, 426
CviQI GTAC 2 cut(s) 120, 388
DdeI CTNAG 1 cut(s) 356
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
Eco130I CCWWGG 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 346
Eco57I CTGAAG 2 cut(s) 128, 399
EcoT14I CCWWGG 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 2 cut(s) 131, 429
FaiI YATR 5 cut(s) 32, 129, 261, 427, 561
FaqI GGGAC 1 cut(s) 411
FatI CATG 2 cut(s) 127, 425
FblI GTMKAC 1 cut(s) 576
FokI GGATG 2 cut(s) 308, 534
FspBI CTAG 3 cut(s) 203, 333, 483
GlaI GCGC 1 cut(s) 103
GsuI CTGGAG 1 cut(s) 627
HaeII RGCGCY 1 cut(s) 105
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 159
HhaI GCGC 1 cut(s) 104
Hin1I GRCGYC 1 cut(s) 151
Hin1II CATG 2 cut(s) 131, 429
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HinfI GANTC 3 cut(s) 251, 324, 459
HpaII CCGG 1 cut(s) 77
HphI GGTGA 2 cut(s) 319, 448
Hpy166II GTNNAC 1 cut(s) 577
Hpy188I TCNGA 1 cut(s) 323
Hpy188III TCNNGA 3 cut(s) 203, 519, 606
Hpy8I GTNNAC 1 cut(s) 577
Hpy99I CGWCG 1 cut(s) 153
HpyAV CCTTC 1 cut(s) 630
HpyCH4IV ACGT 1 cut(s) 505
HpyCH4V TGCA 2 cut(s) 98, 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 479
HpyF3I CTNAG 1 cut(s) 356
HpySE526I ACGT 1 cut(s) 505
Hsp92I GRCGYC 1 cut(s) 151
Hsp92II CATG 2 cut(s) 131, 429
HspAI GCGC 1 cut(s) 102
LmnI GCTCC 4 cut(s) 235, 324, 478, 608
LpnPI CCDG 4 cut(s) 90, 119, 591, 606
MaeI CTAG 3 cut(s) 203, 333, 483
MaeII ACGT 1 cut(s) 505
MaeIII GTNAC 2 cut(s) 88, 212
MboII GAAGA 6 cut(s) 121, 392, 500, 548, 566, 578
MluCI AATT 5 cut(s) 22, 221, 341, 570, 601
MlyI GAGTC 1 cut(s) 318
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 5 cut(s) 62, 226, 314, 388, 396
MroXI GAANNNNTTC 1 cut(s) 507
MseI TTAA 3 cut(s) 240, 422, 455
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 1 cut(s) 77
MwoI GCNNNNNNNGC 2 cut(s) 308, 479
NheI GCTAGC 1 cut(s) 332
NlaIII CATG 2 cut(s) 131, 429
NspI RCATGY 1 cut(s) 131
PdmI GAANNNNTTC 1 cut(s) 507
PfeI GAWTC 2 cut(s) 251, 459
PleI GAGTC 1 cut(s) 318
PpsI GAGTC 1 cut(s) 318
PshAI GACNNNNGTC 1 cut(s) 351
PspPI GGNCC 1 cut(s) 346
RsaI GTAC 2 cut(s) 121, 389
RsaNI GTAC 2 cut(s) 120, 388
SaqAI TTAA 3 cut(s) 240, 422, 455
Sau96I GGNCC 1 cut(s) 346
SchI GAGTC 1 cut(s) 318
SinI GGWCC 1 cut(s) 346
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 5 cut(s) 22, 221, 341, 570, 601
SsiI CCGC 3 cut(s) 66, 330, 434
SspMI CTAG 3 cut(s) 203, 333, 483
StyI CCWWGG 1 cut(s) 349
TaiI ACGT 1 cut(s) 508
TaqI TCGA 1 cut(s) 501
TasI AATT 5 cut(s) 22, 221, 341, 570, 601
TatI WGTACW 2 cut(s) 119, 387
TfiI GAWTC 2 cut(s) 251, 459
Tru1I TTAA 3 cut(s) 240, 422, 455
Tru9I TTAA 3 cut(s) 240, 422, 455
TspDTI ATGAA 4 cut(s) 312, 354, 456, 579
TspGWI ACGGA 3 cut(s) 62, 197, 415
Vha464I CTTAAG 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 346
XapI RAATTY 2 cut(s) 22, 601
XbaI TCTAGA 1 cut(s) 202
XceI RCATGY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 2 cut(s) 262, 423
XmiI GTMKAC 1 cut(s) 576
XmnI GAANNNNTTC 1 cut(s) 507
XspI CTAG 3 cut(s) 203, 333, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.