Rroxscaffold_5G00357900

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
N/A
Physical Location & Seq
Forward (+)
37857523 .. 37865645
8123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00357900.1

Sequence Viewer

Length: 471 bp
ATGCTCCGACCCGTTTTCTACCGAGTGGATCCGTCGGGTGTACGAAATCAGAGAGGTAGCTTTGGGGAGGCTCTTGAGAAACTCGAAACCAGATTCAAAGAAATGAAGGGCGGCTCTTACAGAAGCAGCAAATTTGTCAGGGTGGCCTCTCTTGATCCATGGCAGGGGATGGGATCATTAGAATGTGACTGCCACTATCTTCTGGACTTGAGCTTGACATCAGATCTGATCTTGGACCCTAAAAAAAAGAGGGTTTCTCTACTAATGGCGGCTGCAACCTTTCAAGTGGCTACAGTTGGTTCTCTCATTGATCTGGCCATTACCCAAGTTGGTCAAACTGGTGAGTGTGGGACTGAACTTAACACTGGAGGCCGAACAGTGTGGGCTAACTTGAATTTATGTATACTTTACACAGTGTGGGCTAACTTTGAATTTATATATATATACTTTCCTAAACACAGTGTGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

156

Amino Acids

17.42

Weight (kDa)

8.36

Isoelectric Point (pI)

27.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 403
AciI CCGC 2 cut(s) 111, 269
AclWI GGATC 4 cut(s) 23, 36, 149, 181
AcoI YGGCCR 1 cut(s) 315
AcsI RAATTY 3 cut(s) 131, 394, 431
AdeI CACNNNGTG 2 cut(s) 417, 463
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 164
AgsI TTSAA 4 cut(s) 97, 284, 394, 431
AluBI AGCT 2 cut(s) 60, 213
AluI AGCT 2 cut(s) 60, 213
AlwI GGATC 4 cut(s) 23, 36, 149, 181
AoxI GGCC 3 cut(s) 144, 315, 370
ApeKI GCWGC 2 cut(s) 126, 272
ApoI RAATTY 3 cut(s) 131, 394, 431
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 353
AvaII GGWCC 1 cut(s) 235
BalI TGGCCA 1 cut(s) 317
BamHI GGATCC 1 cut(s) 28
BbvI GCAGC 2 cut(s) 138, 259
BccI CCATC 1 cut(s) 163
BfmI CTRYAG 1 cut(s) 291
BglII AGATCT 1 cut(s) 223
BisI GCNGC 4 cut(s) 112, 127, 270, 273
BlsI GCNGC 4 cut(s) 113, 128, 271, 274
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 2 cut(s) 30, 237
BplI GAGNNNNNCTC 2 cut(s) 241, 273
BpmI CTGGAG 1 cut(s) 387
BpuEI CTTGAG 2 cut(s) 95, 229
BsaJI CCNNGG 1 cut(s) 158
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 343, 370
BseDI CCNNGG 1 cut(s) 158
BseGI GGATG 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 164
BseNI ACTGG 2 cut(s) 343, 370
BseXI GCAGC 2 cut(s) 138, 259
BshFI GGCC 3 cut(s) 146, 317, 372
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 364
BsnI GGCC 3 cut(s) 146, 317, 372
Bsp143I GATC 6 cut(s) 28, 154, 173, 223, 228, 310
Bsp19I CCATGG 1 cut(s) 158
BspACI CCGC 2 cut(s) 111, 269
BspANI GGCC 3 cut(s) 146, 317, 372
BspLI GGNNCC 2 cut(s) 30, 237
BspPI GGATC 4 cut(s) 23, 36, 149, 181
BsrI ACTGG 2 cut(s) 343, 370
BssECI CCNNGG 1 cut(s) 158
BssMI GATC 6 cut(s) 28, 154, 173, 223, 228, 310
BssNAI GTATAC 1 cut(s) 404
BssT1I CCWWGG 1 cut(s) 158
Bst1107I GTATAC 1 cut(s) 404
Bst4CI ACNGT 4 cut(s) 295, 379, 415, 461
BstDSI CCRYGG 1 cut(s) 158
BstF5I GGATG 1 cut(s) 174
BstKTI GATC 6 cut(s) 31, 157, 176, 226, 231, 313
BstMBI GATC 6 cut(s) 28, 154, 173, 223, 228, 310
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 138, 259
BstX2I RGATCY 2 cut(s) 28, 223
BstYI RGATCY 2 cut(s) 28, 223
BstZ17I GTATAC 1 cut(s) 404
BsuRI GGCC 3 cut(s) 146, 317, 372
BtgI CCRYGG 1 cut(s) 158
BtsCI GGATG 1 cut(s) 174
BtsIMutI CAGTG 4 cut(s) 363, 384, 420, 466
Cfr13I GGNCC 1 cut(s) 235
Csp6I GTAC 1 cut(s) 41
CviAII CATG 1 cut(s) 159
CviQI GTAC 1 cut(s) 41
DpnI GATC 6 cut(s) 30, 156, 175, 225, 230, 312
DpnII GATC 6 cut(s) 28, 154, 173, 223, 228, 310
DraIII CACNNNGTG 2 cut(s) 417, 463
EaeI YGGCCR 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 158
ErhI CCWWGG 1 cut(s) 158
FaeI CATG 1 cut(s) 162
FaiI YATR 8 cut(s) 160, 400, 404, 437, 439, 441, 443, 445
FaqI GGGAC 1 cut(s) 364
FatI CATG 1 cut(s) 158
FblI GTMKAC 1 cut(s) 403
Fnu4HI GCNGC 4 cut(s) 112, 127, 270, 273
FokI GGATG 1 cut(s) 181
Fsp4HI GCNGC 4 cut(s) 112, 127, 270, 273
GluI GCNGC 4 cut(s) 112, 127, 270, 273
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 3 cut(s) 146, 317, 372
Hin1II CATG 1 cut(s) 162
HinfI GANTC 1 cut(s) 93
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 2 cut(s) 41, 404
Hpy188I TCNGA 4 cut(s) 8, 51, 223, 228
Hpy188III TCNNGA 3 cut(s) 74, 152, 203
Hpy8I GTNNAC 2 cut(s) 41, 404
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 4 cut(s) 295, 379, 415, 461
HpyCH4V TGCA 1 cut(s) 275
Hsp92II CATG 1 cut(s) 162
Kzo9I GATC 6 cut(s) 28, 154, 173, 223, 228, 310
LmnI GCTCC 1 cut(s) 9
LpnPI CCDG 7 cut(s) 103, 124, 149, 188, 299, 324, 351
Lsp1109I GCAGC 2 cut(s) 138, 259
MaeIII GTNAC 1 cut(s) 185
MalI GATC 6 cut(s) 30, 156, 175, 225, 230, 312
MboI GATC 6 cut(s) 28, 154, 173, 223, 228, 310
MboII GAAGA 1 cut(s) 191
MflI RGATCY 2 cut(s) 28, 223
MlsI TGGCCA 1 cut(s) 317
MluCI AATT 3 cut(s) 131, 394, 431
MluNI TGGCCA 1 cut(s) 317
MmeI TCCRAC 1 cut(s) 31
MnlI CCTC 5 cut(s) 47, 61, 157, 243, 362
Mox20I TGGCCA 1 cut(s) 317
MscI TGGCCA 1 cut(s) 317
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 1 cut(s) 181
Msp20I TGGCCA 1 cut(s) 317
NcoI CCATGG 1 cut(s) 158
NdeII GATC 6 cut(s) 28, 154, 173, 223, 228, 310
NlaIII CATG 1 cut(s) 162
NlaIV GGNNCC 2 cut(s) 30, 237
NmuCI GTSAC 1 cut(s) 185
PfeI GAWTC 1 cut(s) 93
PkrI GCNGC 4 cut(s) 113, 128, 271, 274
PspN4I GGNNCC 2 cut(s) 30, 237
PspPI GGNCC 1 cut(s) 235
PsuI RGATCY 2 cut(s) 28, 223
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
RseI CAYNNNNRTG 1 cut(s) 181
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 4 cut(s) 112, 127, 270, 273
Sau3AI GATC 6 cut(s) 28, 154, 173, 223, 228, 310
Sau96I GGNCC 1 cut(s) 235
SetI ASST 4 cut(s) 58, 62, 215, 281
SfcI CTRYAG 1 cut(s) 291
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 181
SmlI CTYRAG 2 cut(s) 74, 208
SmoI CTYRAG 2 cut(s) 74, 208
Sse9I AATT 3 cut(s) 131, 394, 431
SsiI CCGC 2 cut(s) 111, 269
StyI CCWWGG 1 cut(s) 158
TaaI ACNGT 4 cut(s) 295, 379, 415, 461
TaqI TCGA 1 cut(s) 84
TasI AATT 3 cut(s) 131, 394, 431
TauI GCSGC 2 cut(s) 114, 272
TfiI GAWTC 1 cut(s) 93
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 4 cut(s) 370, 384, 420, 466
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 2 cut(s) 126, 272
Tsp45I GTSAC 1 cut(s) 185
TspDTI ATGAA 1 cut(s) 119
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 4 cut(s) 370, 384, 420, 466
VpaK11BI GGWCC 1 cut(s) 235
XapI RAATTY 3 cut(s) 131, 394, 431
XmiI GTMKAC 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.