Rroxscaffold_5G00374700

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
N/A
Physical Location & Seq
Reverse (-)
55686282 .. 55687018
737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00374700.1

Sequence Viewer

Length: 600 bp
ATGGAGGACATTATGCCATGCTTGGTGCACATATCTCCTAATGCTCGGCATGCGTCTTTGCTTTATGGAAATACTCAACTATCTACATTGGAGACGTTTTACAAGTATAAGAAGTTGAGGACATTTACATTGCTTCCTAAATCCCCATCTATCCCGAGGCCATTCATACCGTTATTGAACTCTTCGAAGCCTATAACAACAATATATGGGTTAGAGACATTGAAGCTCGAGAATTGTCCTAAGCTTCTTCATTTTCCCGAGAACTTAAAGGACTTGATTAAGCTAAGACATCTTGACTTTGACGGATCCTTCATGCCAATATATGTTGGGAAGTTAACTAGTCTTGAAACCCTTCATGCATTTAGAGTGGGAAAAGAGAAAGGATATCGGATTGAGGAGCTTAAGAACATGAGGTGTCTTCGTGGATCCATTTCTATTACGAATCTTGAGAATGTGGCAAATTCTATGCAGGATGAGGCTGCTATGTTACACAACAAGCAATACCGAGACAGTTTGGAGCTGAAATGGAACGGAACCGGAGGTCAAACGCAACCGGTTCAACAAAGCGGTTCTTGCGAGCTTGAACCACATGCGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

199

Amino Acids

22.6

Weight (kDa)

8.83

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 68 - 158 4.1e-07 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 131 - 185 1.5e-10 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 567, 593
AclWI GGATC 4 cut(s) 300, 313, 420, 433
AcsI RAATTY 1 cut(s) 460
AfiI CCNNNNNNNGG 1 cut(s) 593
AflII CTTAAG 1 cut(s) 401
AgeI ACCGGT 1 cut(s) 553
AgsI TTSAA 5 cut(s) 178, 223, 347, 560, 584
AhlI ACTAGT 1 cut(s) 338
AluBI AGCT 6 cut(s) 226, 244, 283, 400, 520, 580
AluI AGCT 6 cut(s) 226, 244, 283, 400, 520, 580
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 3 cut(s) 86, 209, 501
Alw44I GTGCAC 1 cut(s) 26
AlwI GGATC 4 cut(s) 300, 313, 420, 433
Ama87I CYCGRG 3 cut(s) 154, 227, 257
AoxI GGCC 1 cut(s) 158
ApaLI GTGCAC 1 cut(s) 26
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 1 cut(s) 460
AsiGI ACCGGT 1 cut(s) 553
Asp700I GAANNNNTTC 1 cut(s) 351
AsuII TTCGAA 1 cut(s) 185
AvaI CYCGRG 3 cut(s) 154, 227, 257
BaeGI GKGCMC 1 cut(s) 30
BamHI GGATCC 2 cut(s) 305, 425
BbsI GAAGAC 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 466
BccI CCATC 1 cut(s) 154
BcoDI GTCTC 3 cut(s) 86, 209, 501
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 1 cut(s) 339
BfrI CTTAAG 1 cut(s) 401
BisI GCNGC 1 cut(s) 480
BlsI GCNGC 1 cut(s) 481
BmeT110I CYCGRG 3 cut(s) 154, 227, 257
BmiI GGNNCC 3 cut(s) 307, 427, 535
BpiI GAAGAC 1 cut(s) 410
Bpu10I CCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 467
BsaJI CCNNGG 1 cut(s) 155
BsaWI WCCGGW 2 cut(s) 536, 553
Bsc4I CCNNNNNNNGG 1 cut(s) 593
Bse118I RCCGGY 1 cut(s) 553
Bse3DI GCAATG 1 cut(s) 128
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 1 cut(s) 478
BseLI CCNNNNNNNGG 1 cut(s) 593
BseMI GCAATG 1 cut(s) 128
BseRI GAGGAG 1 cut(s) 410
BseSI GKGCMC 1 cut(s) 30
BseXI GCAGC 1 cut(s) 466
BshFI GGCC 1 cut(s) 160
BshTI ACCGGT 1 cut(s) 553
BsiHKAI GWGCWC 1 cut(s) 30
BsiHKCI CYCGRG 3 cut(s) 154, 227, 257
BsiSI CCGG 2 cut(s) 537, 554
BslI CCNNNNNNNGG 1 cut(s) 593
BsmAI GTCTC 3 cut(s) 86, 209, 501
BsmBI CGTCTC 1 cut(s) 86
BsnI GGCC 1 cut(s) 160
BsoBI CYCGRG 3 cut(s) 154, 227, 257
Bsp119I TTCGAA 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 2 cut(s) 305, 425
BspACI CCGC 2 cut(s) 567, 593
BspANI GGCC 1 cut(s) 160
BspLI GGNNCC 3 cut(s) 307, 427, 535
BspPI GGATC 4 cut(s) 300, 313, 420, 433
BspT104I TTCGAA 1 cut(s) 185
BspTI CTTAAG 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 553
BssAI RCCGGY 1 cut(s) 553
BssECI CCNNGG 1 cut(s) 155
BssMI GATC 2 cut(s) 305, 425
Bst4CI ACNGT 2 cut(s) 171, 512
Bst6I CTCTTC 1 cut(s) 187
BstAFI CTTAAG 1 cut(s) 401
BstBI TTCGAA 1 cut(s) 185
BstC8I GCNNGC 2 cut(s) 51, 578
BstDEI CTNAG 2 cut(s) 240, 284
BstF5I GGATG 1 cut(s) 478
BstKTI GATC 2 cut(s) 308, 428
BstMAI GTCTC 3 cut(s) 86, 209, 501
BstMBI GATC 2 cut(s) 305, 425
BstMWI GCNNNNNNNGC 2 cut(s) 50, 573
BstNSI RCATGY 2 cut(s) 53, 593
BstSLI GKGCMC 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 466
BstV2I GAAGAC 1 cut(s) 410
BstX2I RGATCY 2 cut(s) 305, 425
BstYI RGATCY 2 cut(s) 305, 425
BsuRI GGCC 1 cut(s) 160
BtsCI GGATG 1 cut(s) 478
Cac8I GCNNGC 2 cut(s) 51, 578
Cfr10I RCCGGY 1 cut(s) 553
CseI GACGC 1 cut(s) 42
CspAI ACCGGT 1 cut(s) 553
CviAII CATG 6 cut(s) 18, 50, 313, 356, 409, 590
CviJI RGCY 9 cut(s) 160, 190, 226, 244, 283, 400, 479, 520, 580
CviKI_1 RGCY 9 cut(s) 160, 190, 226, 244, 283, 400, 479, 520, 580
DdeI CTNAG 2 cut(s) 240, 284
DpnI GATC 2 cut(s) 307, 427
DpnII GATC 2 cut(s) 305, 425
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
Eco32I GATATC 1 cut(s) 386
Eco88I CYCGRG 3 cut(s) 154, 227, 257
EcoRV GATATC 1 cut(s) 386
EcoT22I ATGCAT 1 cut(s) 361
Esp3I CGTCTC 1 cut(s) 86
FaeI CATG 6 cut(s) 21, 53, 316, 359, 412, 593
FalI AAGNNNNNCTT 2 cut(s) 556, 588
FatI CATG 6 cut(s) 17, 49, 312, 355, 408, 589
FauI CCCGC 1 cut(s) 586
Fnu4HI GCNGC 1 cut(s) 480
FokI GGATG 1 cut(s) 485
Fsp4HI GCNGC 1 cut(s) 480
FspBI CTAG 1 cut(s) 339
GluI GCNGC 1 cut(s) 480
HaeIII GGCC 1 cut(s) 160
HapII CCGG 2 cut(s) 537, 554
HgaI GACGC 1 cut(s) 42
Hin1II CATG 6 cut(s) 21, 53, 316, 359, 412, 593
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 1 cut(s) 442
HpaI GTTAAC 1 cut(s) 336
HpaII CCGG 2 cut(s) 537, 554
Hpy166II GTNNAC 2 cut(s) 28, 336
Hpy188I TCNGA 1 cut(s) 390
Hpy188III TCNNGA 6 cut(s) 154, 229, 257, 293, 344, 446
Hpy8I GTNNAC 2 cut(s) 28, 336
HpyAV CCTTC 2 cut(s) 319, 362
HpyCH4III ACNGT 2 cut(s) 171, 512
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 3 cut(s) 28, 359, 469
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 573
HpyF3I CTNAG 2 cut(s) 240, 284
HpySE526I ACGT 1 cut(s) 95
Hsp92II CATG 6 cut(s) 21, 53, 316, 359, 412, 593
KspAI GTTAAC 1 cut(s) 336
Kzo9I GATC 2 cut(s) 305, 425
LmnI GCTCC 2 cut(s) 397, 517
LpnPI CCDG 3 cut(s) 455, 550, 567
Lsp1109I GCAGC 1 cut(s) 466
MaeI CTAG 1 cut(s) 339
MaeII ACGT 1 cut(s) 95
MaeIII GTNAC 1 cut(s) 486
MalI GATC 2 cut(s) 307, 427
MboI GATC 2 cut(s) 305, 425
MboII GAAGA 3 cut(s) 174, 239, 410
MflI RGATCY 2 cut(s) 305, 425
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 2 cut(s) 232, 460
MnlI CCTC 6 cut(s) 111, 150, 388, 405, 469, 533
Mph1103I ATGCAT 1 cut(s) 361
MroXI GAANNNNTTC 1 cut(s) 351
MseI TTAA 4 cut(s) 266, 279, 335, 402
MspCI CTTAAG 1 cut(s) 401
MspI CCGG 2 cut(s) 537, 554
MwoI GCNNNNNNNGC 2 cut(s) 50, 573
NdeII GATC 2 cut(s) 305, 425
NlaIII CATG 6 cut(s) 21, 53, 316, 359, 412, 593
NlaIV GGNNCC 3 cut(s) 307, 427, 535
NmeAIII GCCGAG 1 cut(s) 25
NsiI ATGCAT 1 cut(s) 361
NspI RCATGY 2 cut(s) 53, 593
NspV TTCGAA 1 cut(s) 185
PaeI GCATGC 1 cut(s) 53
PaeR7I CTCGAG 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 351
PfeI GAWTC 1 cut(s) 442
PinAI ACCGGT 1 cut(s) 553
PkrI GCNGC 1 cut(s) 481
PspN4I GGNNCC 3 cut(s) 307, 427, 535
PsuI RGATCY 2 cut(s) 305, 425
SaqAI TTAA 4 cut(s) 266, 279, 335, 402
SatI GCNGC 1 cut(s) 480
Sau3AI GATC 2 cut(s) 305, 425
SduI GDGCHC 1 cut(s) 30
SetI ASST 9 cut(s) 98, 228, 246, 285, 402, 416, 522, 544, 582
Sfr274I CTCGAG 1 cut(s) 227
SfuI TTCGAA 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 227
SmlI CTYRAG 3 cut(s) 227, 401, 446
SmoI CTYRAG 3 cut(s) 227, 401, 446
SpeI ACTAGT 1 cut(s) 338
SphI GCATGC 1 cut(s) 53
Sse9I AATT 2 cut(s) 232, 460
SsiI CCGC 2 cut(s) 567, 593
SspMI CTAG 1 cut(s) 339
TaaI ACNGT 2 cut(s) 171, 512
TaiI ACGT 1 cut(s) 98
TaqI TCGA 2 cut(s) 185, 228
TasI AATT 2 cut(s) 232, 460
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 4 cut(s) 266, 279, 335, 402
Tru9I TTAA 4 cut(s) 266, 279, 335, 402
TseI GCWGC 1 cut(s) 479
TspDTI ATGAA 4 cut(s) 154, 239, 301, 344
TspGWI ACGGA 2 cut(s) 318, 546
Vha464I CTTAAG 1 cut(s) 401
VneI GTGCAC 1 cut(s) 26
XapI RAATTY 1 cut(s) 460
XceI RCATGY 2 cut(s) 53, 593
XhoI CTCGAG 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 351
XspI CTAG 1 cut(s) 339
Zsp2I ATGCAT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.