Rroxscaffold_6G00398180
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
N/A
Physical Location & Seq
Reverse (-)
20012014 .. 20015306
3293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00398180.1

Sequence Viewer

Length: 750 bp
ATGATCGGTCCCGTACGCATGTCCACCGCAGCCTTCTTCGCTTCGAGATCTCCTCCGGCTCACCGGCTTCTCCGCCACGGCCACGGCTCCGGCTCGCCACGTTGGACGGCTCCTCCGAGGTTTCTGGGCCGACTCGTCCTCGTCAACGGCTCGGATCCGGTCGCAGGTCGGATGGCTTGTGGGTATTCCAAATTGGAGAGTTTCGGTTGGGGGTGGCGAGTCCGATGGAGTTTGGCCCCAAAGTTGAAGGCTTGGAGGTCAACTAGTGCTAGGTTCAAGAGCAACCAGTGGTTTCAGTGTATCAAATGTGTTGGGAGAAGGGGAGGTGTAAAAGGGTACTGCGATAATTGTGAAAAGGATAAGGGGGTCAAGGCTGAGATTACCTTACTTTGTGGACTTGGTCATCAAAACTTGGTTGCTCTACTTATTGTTCCATTGGAAGCAAGAAAACCTAAGCCTTCTGGATTGGCCTACAAGACTCAATTGTCTTGGATATTGCAAATGGACACTGCAAGCACCGAGGCCTTTGTTCACCGGGACCTGAAGCCCTCAAACATATTACTTGCACATGATATGGAGGCTAAGATAATATGGAGTCGTCTTGTGGAGCTCATTAGGAGCAGAAAGTCGATCATGGAGATCGTGTATGATGCTGAGAAATCATTACCTACTTTGCTGACAGCCTCAATGGTAAAATCATTCAGCTTGTTGATATTTCTAGGCTTAGCACCATTGTTTTTCAACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

249

Amino Acids

27.84

Weight (kDa)

10.29

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 484
Acc36I ACCTGC 1 cut(s) 155
AciI CCGC 2 cut(s) 27, 73
AclWI GGATC 2 cut(s) 149, 162
AcoI YGGCCR 1 cut(s) 79
AcuI CTGAAG 1 cut(s) 563
AfaI GTAC 2 cut(s) 15, 338
AfiI CCNNNNNNNGG 1 cut(s) 164
AgsI TTSAA 3 cut(s) 247, 277, 742
AhlI ACTAGT 1 cut(s) 263
AluBI AGCT 2 cut(s) 610, 705
AluI AGCT 2 cut(s) 610, 705
Alw21I GWGCWC 1 cut(s) 612
AlwI GGATC 2 cut(s) 149, 162
AoxI GGCC 5 cut(s) 79, 127, 234, 468, 522
ApeKI GCWGC 1 cut(s) 29
AspS9I GGNCC 4 cut(s) 8, 127, 235, 538
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 2 cut(s) 53, 524
AvaII GGWCC 2 cut(s) 8, 538
BamHI GGATCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 612
BbvI GCAGC 1 cut(s) 41
BccI CCATC 2 cut(s) 166, 219
BceAI ACGGC 4 cut(s) 94, 100, 123, 163
BcnI CCSGG 1 cut(s) 536
BcuI ACTAGT 1 cut(s) 263
BfaI CTAG 3 cut(s) 264, 270, 719
BfuAI ACCTGC 1 cut(s) 155
BglII AGATCT 1 cut(s) 47
BisI GCNGC 1 cut(s) 30
BlpI GCTNAGC 1 cut(s) 724
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 1 cut(s) 536
Bme18I GGWCC 2 cut(s) 8, 538
BmgT120I GGNCC 4 cut(s) 8, 127, 235, 538
BmiI GGNNCC 6 cut(s) 10, 88, 111, 156, 237, 539
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 1 cut(s) 640
BplI GAGNNNNNCTC 2 cut(s) 37, 69
Bpu10I CCTNAGC 1 cut(s) 453
Bpu1102I GCTNAGC 1 cut(s) 724
BpuMI CCSGG 1 cut(s) 536
BsaJI CCNNGG 4 cut(s) 76, 82, 116, 519
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 4 cut(s) 97, 127, 629, 659
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse118I RCCGGY 1 cut(s) 63
Bse1I ACTGG 1 cut(s) 286
BseDI CCNNGG 4 cut(s) 76, 82, 116, 519
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMII CTCAG 2 cut(s) 366, 645
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 2 cut(s) 42, 102
BseXI GCAGC 1 cut(s) 41
Bsh1285I CGRYCG 1 cut(s) 162
BshFI GGCC 5 cut(s) 81, 129, 236, 470, 524
BsiEI CGRYCG 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 5 cut(s) 56, 64, 90, 158, 535
BsiWI CGTACG 1 cut(s) 13
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 551
BsnI GGCC 5 cut(s) 81, 129, 236, 470, 524
Bsp1286I GDGCHC 1 cut(s) 612
Bsp143I GATC 5 cut(s) 3, 47, 154, 630, 639
Bsp1720I GCTNAGC 1 cut(s) 724
BspACI CCGC 2 cut(s) 27, 73
BspANI GGCC 5 cut(s) 81, 129, 236, 470, 524
BspCNI CTCAG 2 cut(s) 367, 646
BspLI GGNNCC 6 cut(s) 10, 88, 111, 156, 237, 539
BspMI ACCTGC 1 cut(s) 155
BspPI GGATC 2 cut(s) 149, 162
BsrFI RCCGGY 1 cut(s) 63
BsrI ACTGG 1 cut(s) 286
BssAI RCCGGY 1 cut(s) 63
BssECI CCNNGG 4 cut(s) 76, 82, 116, 519
BssMI GATC 5 cut(s) 3, 47, 154, 630, 639
Bst4CI ACNGT 1 cut(s) 746
BstC8I GCNNGC 2 cut(s) 95, 514
BstDEI CTNAG 5 cut(s) 375, 453, 582, 654, 724
BstDSI CCRYGG 2 cut(s) 76, 82
BstF5I GGATG 1 cut(s) 177
BstKTI GATC 5 cut(s) 6, 50, 157, 633, 642
BstMBI GATC 5 cut(s) 3, 47, 154, 630, 639
BstMCI CGRYCG 1 cut(s) 162
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNSI RCATGY 1 cut(s) 22
BstSCI CCNGG 1 cut(s) 534
BstV1I GCAGC 1 cut(s) 41
BstX2I RGATCY 2 cut(s) 47, 154
BstYI RGATCY 2 cut(s) 47, 154
BsuRI GGCC 5 cut(s) 81, 129, 236, 470, 524
BtgI CCRYGG 2 cut(s) 76, 82
BtsCI GGATG 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 3 cut(s) 293, 302, 507
BveI ACCTGC 1 cut(s) 155
Cac8I GCNNGC 2 cut(s) 95, 514
Cfr10I RCCGGY 1 cut(s) 63
Cfr13I GGNCC 4 cut(s) 8, 127, 235, 538
Csp6I GTAC 2 cut(s) 14, 337
CviAII CATG 3 cut(s) 19, 569, 634
CviQI GTAC 2 cut(s) 14, 337
DdeI CTNAG 5 cut(s) 375, 453, 582, 654, 724
DpnI GATC 5 cut(s) 5, 49, 156, 632, 641
DpnII GATC 5 cut(s) 3, 47, 154, 630, 639
DrdI GACNNNNNNGTC 1 cut(s) 484
DseDI GACNNNNNNGTC 1 cut(s) 484
EaeI YGGCCR 1 cut(s) 79
EciI GGCGGA 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 610
Eco147I AGGCCT 1 cut(s) 524
Eco24I GRGCYC 1 cut(s) 612
Eco47I GGWCC 2 cut(s) 8, 538
Eco53kI GAGCTC 1 cut(s) 610
Eco57I CTGAAG 1 cut(s) 563
EcoICRI GAGCTC 1 cut(s) 610
EcoO109I RGGNCCY 1 cut(s) 538
EcoT38I GRGCYC 1 cut(s) 612
FaeI CATG 3 cut(s) 22, 572, 637
FaiI YATR 7 cut(s) 20, 557, 570, 575, 592, 635, 648
FaqI GGGAC 1 cut(s) 551
FatI CATG 3 cut(s) 18, 568, 633
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 1 cut(s) 184
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 3 cut(s) 264, 270, 719
GluI GCNGC 1 cut(s) 30
HaeIII GGCC 5 cut(s) 81, 129, 236, 470, 524
HapII CCGG 5 cut(s) 56, 64, 90, 158, 535
Hin1II CATG 3 cut(s) 22, 572, 637
HincII GTYRAC 2 cut(s) 145, 261
HindII GTYRAC 2 cut(s) 145, 261
HinfI GANTC 4 cut(s) 132, 219, 478, 595
HpaII CCGG 5 cut(s) 56, 64, 90, 158, 535
HphI GGTGA 2 cut(s) 53, 524
Hpy166II GTNNAC 5 cut(s) 24, 145, 261, 395, 532
Hpy188I TCNGA 4 cut(s) 117, 154, 171, 224
Hpy188III TCNNGA 3 cut(s) 45, 277, 462
Hpy8I GTNNAC 5 cut(s) 24, 145, 261, 395, 532
HpyAV CCTTC 4 cut(s) 43, 241, 312, 468
HpyCH4III ACNGT 1 cut(s) 746
HpyCH4IV ACGT 1 cut(s) 100
HpyCH4V TGCA 3 cut(s) 499, 512, 566
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 5 cut(s) 375, 453, 582, 654, 724
HpySE526I ACGT 1 cut(s) 100
Hsp92II CATG 3 cut(s) 22, 572, 637
Kzo9I GATC 5 cut(s) 3, 47, 154, 630, 639
LmnI GCTCC 4 cut(s) 92, 115, 607, 618
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 1 cut(s) 640
MaeI CTAG 3 cut(s) 264, 270, 719
MaeII ACGT 1 cut(s) 100
MalI GATC 5 cut(s) 5, 49, 156, 632, 641
MboI GATC 5 cut(s) 3, 47, 154, 630, 639
MboII GAAGA 1 cut(s) 28
MfeI CAATTG 1 cut(s) 482
MflI RGATCY 2 cut(s) 47, 154
MhlI GDGCHC 1 cut(s) 612
MluCI AATT 3 cut(s) 191, 346, 482
MlyI GAGTC 4 cut(s) 126, 228, 472, 604
MmeI TCCRAC 2 cut(s) 83, 149
MspI CCGG 5 cut(s) 56, 64, 90, 158, 535
MspR9I CCNGG 1 cut(s) 536
MunI CAATTG 1 cut(s) 482
MwoI GCNNNNNNNGC 1 cut(s) 38
NciI CCSGG 1 cut(s) 536
NdeII GATC 5 cut(s) 3, 47, 154, 630, 639
NlaIII CATG 3 cut(s) 22, 572, 637
NlaIV GGNNCC 6 cut(s) 10, 88, 111, 156, 237, 539
NspI RCATGY 1 cut(s) 22
PceI AGGCCT 1 cut(s) 524
PcsI WCGNNNNNNNCGW 1 cut(s) 113
Pfl23II CGTACG 1 cut(s) 13
PflFI GACNNNGTC 1 cut(s) 399
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 4 cut(s) 126, 227, 472, 603
PpsI GAGTC 4 cut(s) 126, 227, 472, 603
PpuMI RGGWCCY 1 cut(s) 538
Psp124BI GAGCTC 1 cut(s) 612
Psp5II RGGWCCY 1 cut(s) 538
PspLI CGTACG 1 cut(s) 13
PspN4I GGNNCC 6 cut(s) 10, 88, 111, 156, 237, 539
PspPI GGNCC 4 cut(s) 8, 127, 235, 538
PspPPI RGGWCCY 1 cut(s) 538
PsuI RGATCY 2 cut(s) 47, 154
PsyI GACNNNGTC 1 cut(s) 399
RsaI GTAC 2 cut(s) 15, 338
RsaNI GTAC 2 cut(s) 14, 337
SacI GAGCTC 1 cut(s) 612
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 5 cut(s) 3, 47, 154, 630, 639
Sau96I GGNCC 4 cut(s) 8, 127, 235, 538
SchI GAGTC 4 cut(s) 126, 228, 472, 604
ScrFI CCNGG 1 cut(s) 536
SduI GDGCHC 1 cut(s) 612
SfaNI GCATC 1 cut(s) 640
SinI GGWCC 2 cut(s) 8, 538
SpeI ACTAGT 1 cut(s) 263
Sse9I AATT 3 cut(s) 191, 346, 482
SseBI AGGCCT 1 cut(s) 524
SsiI CCGC 2 cut(s) 27, 73
SspMI CTAG 3 cut(s) 264, 270, 719
SstI GAGCTC 1 cut(s) 612
StuI AGGCCT 1 cut(s) 524
StyD4I CCNGG 1 cut(s) 534
TaaI ACNGT 1 cut(s) 746
TaiI ACGT 1 cut(s) 103
TaqI TCGA 2 cut(s) 44, 629
TasI AATT 3 cut(s) 191, 346, 482
TscAI CASTG 3 cut(s) 293, 302, 514
TseI GCWGC 1 cut(s) 29
TspRI CASTG 3 cut(s) 293, 302, 514
Tth111I GACNNNGTC 1 cut(s) 399
VpaK11BI GGWCC 2 cut(s) 8, 538
XceI RCATGY 1 cut(s) 22
XspI CTAG 3 cut(s) 264, 270, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.