Rroxscaffold_7G00200460

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
N/A
Physical Location & Seq
Forward (+)
47866757 .. 47868028
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00200460.1

Sequence Viewer

Length: 909 bp
ATGGATTCTGTTTCTACTCAAGGGGCCTCTTCTTCCTCTTCTCCTTCTTCCTCCCATTCATTGACATACGATGCGTTCTTGAGTATTAGAAGTGAGGATAGAGGCAGCAAATTCGCACGCAAATTACACAGAAATCTGGTTGGGAAGGGGAGAACACCTTCAATCATGGCATTCAGGATGCAGAGGGACATGCAAAAGCAATTGAAGAATCAAAAAGAAAGTAACCATTTTGTTAGATATCTCATATGTGGTTCATCTGGCACGAGTCAATCCAAATTCATTGATAGCATCATTGAAGAGATTTTGGCACAATCGTTAAGACATACTAACTTAAAACGTACCTACTTACCTGTGGCAAAGCATCCTGTTGGAATAGAGTCTCGCATACAAGATATGAAATTTTTAGAAGTTGAGAAATGTGATGTCCGTATGGTAGGGATACTTGGAACTGGTGGTATAGGCAAGACAGCAGTTGCTAAAGCTGTGTATAATTCTATTGCTTATCGGCTTCAAGGAGGTTGTTTTCTAGCAAATATTGGGGATGTGGAAAAGTTCAACCGATGTGAAGGTCTATCAATTGATTCAATTCTACAAACCATTCTTCGTTCTAGGATTTTAGGTAGCAAAGTAAATGTAACCGATGTTGACCAAGGAATCACTGTGATAAAGAAGAGATTGAGGCATAAAAGGGTCGTCTTAATTCTTGATAATGTGAATCATCCAGACCAGTTAAACAAATTAGTTGGAGGTTTAAATTGGTCTGGTTCGGGTAGCAGAGTTATCATAACAACAAGATATAGGAATTTGCTTGTTGCTCATCAAGTTAATCGAATATACACGGTCAATAAACTAAATCGAGATGAAGCAATGCAGCTCCTGTTGGAATGCCTTCAGAGGAGGTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

302

Amino Acids

33.83

Weight (kDa)

10.1

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 137 - 294 3.6e-15 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 4 cut(s) 110, 275, 398, 802
AcuI CTGAAG 1 cut(s) 875
AfaI GTAC 1 cut(s) 340
AgsI TTSAA 6 cut(s) 162, 205, 296, 512, 556, 585
AluBI AGCT 2 cut(s) 482, 874
AluI AGCT 2 cut(s) 482, 874
Alw26I GTCTC 1 cut(s) 384
AlwNI CAGNNNCTG 1 cut(s) 877
AoxI GGCC 1 cut(s) 24
ApeKI GCWGC 2 cut(s) 105, 871
ApoI RAATTY 4 cut(s) 110, 275, 398, 802
Asp700I GAANNNNTTC 2 cut(s) 157, 888
AspS9I GGNCC 1 cut(s) 24
BauI CACGAG 1 cut(s) 262
BbvI GCAGC 2 cut(s) 117, 883
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciVI GTATCC 1 cut(s) 432
BcoDI GTCTC 1 cut(s) 384
BfaI CTAG 2 cut(s) 527, 609
BfuI GTATCC 1 cut(s) 432
BisI GCNGC 2 cut(s) 106, 872
BlsI GCNGC 2 cut(s) 107, 873
BmgT120I GGNCC 1 cut(s) 24
BmiI GGNNCC 1 cut(s) 25
BmsI GCATC 4 cut(s) 61, 168, 297, 370
BpuEI CTTGAG 1 cut(s) 100
BsaJI CCNNGG 1 cut(s) 649
Bse1I ACTGG 2 cut(s) 454, 727
Bse3DI GCAATG 1 cut(s) 873
BseDI CCNNGG 1 cut(s) 649
BseGI GGATG 4 cut(s) 183, 361, 547, 718
BseMI GCAATG 1 cut(s) 873
BseNI ACTGG 2 cut(s) 454, 727
BseXI GCAGC 2 cut(s) 117, 883
BshFI GGCC 1 cut(s) 26
BslFI GGGAC 1 cut(s) 200
BsmAI GTCTC 1 cut(s) 384
BsmFI GGGAC 1 cut(s) 200
BsmI GAATGC 2 cut(s) 170, 890
BsnI GGCC 1 cut(s) 26
BspANI GGCC 1 cut(s) 26
BspLI GGNNCC 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 873
BsrI ACTGG 2 cut(s) 454, 727
BssECI CCNNGG 1 cut(s) 649
BssSI CACGAG 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 649
Bst2BI CACGAG 1 cut(s) 262
Bst4CI ACNGT 2 cut(s) 661, 841
Bst6I CTCTTC 4 cut(s) 34, 43, 291, 665
BstC8I GCNNGC 1 cut(s) 118
BstF5I GGATG 4 cut(s) 183, 361, 547, 718
BstMAI GTCTC 1 cut(s) 384
BstNSI RCATGY 1 cut(s) 193
BstV1I GCAGC 2 cut(s) 117, 883
BsuI GTATCC 1 cut(s) 432
BsuRI GGCC 1 cut(s) 26
BtsCI GGATG 4 cut(s) 183, 361, 547, 718
BtsIMutI CAGTG 1 cut(s) 657
Cac8I GCNNGC 1 cut(s) 118
CaiI CAGNNNCTG 1 cut(s) 877
Cfr13I GGNCC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 339
CviAII CATG 2 cut(s) 166, 190
CviJI RGCY 4 cut(s) 26, 482, 508, 874
CviKI_1 RGCY 4 cut(s) 26, 482, 508, 874
CviQI GTAC 1 cut(s) 339
DraI TTTAAA 1 cut(s) 753
Eam1104I CTCTTC 4 cut(s) 34, 43, 291, 665
EarI CTCTTC 4 cut(s) 34, 43, 291, 665
Eco130I CCWWGG 1 cut(s) 649
Eco32I GATATC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 875
EcoO109I RGGNCCY 1 cut(s) 24
EcoRV GATATC 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 649
ErhI CCWWGG 1 cut(s) 649
FaeI CATG 2 cut(s) 169, 193
FaqI GGGAC 1 cut(s) 200
FatI CATG 2 cut(s) 165, 189
FauNDI CATATG 1 cut(s) 245
Fnu4HI GCNGC 2 cut(s) 106, 872
FokI GGATG 4 cut(s) 190, 348, 554, 705
Fsp4HI GCNGC 2 cut(s) 106, 872
FspBI CTAG 2 cut(s) 527, 609
GluI GCNGC 2 cut(s) 106, 872
HaeIII GGCC 1 cut(s) 26
Hin1II CATG 2 cut(s) 169, 193
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 7 cut(s) 5, 208, 265, 377, 581, 654, 715
Hpy166II GTNNAC 1 cut(s) 646
Hpy188I TCNGA 1 cut(s) 894
Hpy188III TCNNGA 5 cut(s) 79, 175, 704, 722, 857
Hpy8I GTNNAC 1 cut(s) 646
HpyAV CCTTC 5 cut(s) 54, 139, 168, 560, 899
HpyCH4III ACNGT 2 cut(s) 661, 841
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 3 cut(s) 181, 193, 871
HpySE526I ACGT 1 cut(s) 337
Hsp92II CATG 2 cut(s) 169, 193
LmnI GCTCC 1 cut(s) 879
Lsp1109I GCAGC 2 cut(s) 117, 883
LweI GCATC 4 cut(s) 61, 168, 297, 370
MaeI CTAG 2 cut(s) 527, 609
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 2 cut(s) 221, 634
MboII GAAGA 8 cut(s) 21, 24, 30, 39, 217, 308, 593, 682
MfeI CAATTG 2 cut(s) 200, 576
MlyI GAGTC 2 cut(s) 274, 386
MmeI TCCRAC 3 cut(s) 349, 724, 861
MroXI GAANNNNTTC 2 cut(s) 157, 888
MseI TTAA 6 cut(s) 317, 332, 698, 731, 752, 825
MunI CAATTG 2 cut(s) 200, 576
Mva1269I GAATGC 2 cut(s) 170, 890
NdeI CATATG 1 cut(s) 245
NlaIII CATG 2 cut(s) 169, 193
NlaIV GGNNCC 1 cut(s) 25
NspI RCATGY 1 cut(s) 193
PctI GAATGC 2 cut(s) 170, 890
PdmI GAANNNNTTC 2 cut(s) 157, 888
PfeI GAWTC 5 cut(s) 5, 208, 581, 654, 715
PkrI GCNGC 2 cut(s) 107, 873
PleI GAGTC 2 cut(s) 273, 385
PpsI GAGTC 2 cut(s) 273, 385
PspN4I GGNNCC 1 cut(s) 25
PspPI GGNCC 1 cut(s) 24
PstNI CAGNNNCTG 1 cut(s) 877
RsaI GTAC 1 cut(s) 340
RsaNI GTAC 1 cut(s) 339
SaqAI TTAA 6 cut(s) 317, 332, 698, 731, 752, 825
SatI GCNGC 2 cut(s) 106, 872
Sau96I GGNCC 1 cut(s) 24
SchI GAGTC 2 cut(s) 274, 386
SfaNI GCATC 4 cut(s) 61, 168, 297, 370
SmlI CTYRAG 2 cut(s) 18, 79
SmoI CTYRAG 2 cut(s) 18, 79
SspI AATATT 1 cut(s) 535
SspMI CTAG 2 cut(s) 527, 609
StyI CCWWGG 1 cut(s) 649
TaaI ACNGT 2 cut(s) 661, 841
TaiI ACGT 1 cut(s) 340
TaqI TCGA 2 cut(s) 829, 856
TfiI GAWTC 5 cut(s) 5, 208, 581, 654, 715
Tru1I TTAA 6 cut(s) 317, 332, 698, 731, 752, 825
Tru9I TTAA 6 cut(s) 317, 332, 698, 731, 752, 825
TscAI CASTG 1 cut(s) 664
TseI GCWGC 2 cut(s) 105, 871
TspDTI ATGAA 5 cut(s) 48, 243, 268, 410, 876
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 1 cut(s) 664
XapI RAATTY 4 cut(s) 110, 275, 398, 802
XceI RCATGY 1 cut(s) 193
XmnI GAANNNNTTC 2 cut(s) 157, 888
XspI CTAG 2 cut(s) 527, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.