Rroxscaffold_7G00202590

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
N/A
Physical Location & Seq
Forward (+)
50734405 .. 50774358
39954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00202590.1

Sequence Viewer

Length: 1284 bp
ATGGTTAGACCAGTTTTTTACAAGGTTGATCTCTCGGATATACGACACCACAAAGTCGGTTTCGGTGATGCCTTGGCTAAGCATGAAAGGAGGTTAAGGGAGAACCTGGAGAAGGTGAAGTTATGGAGGATAACACTTACAAAAGCGGCTAATTTGTCTGGTGACCATTTCAAGGAGCGGGATAATGAAATTAAGTTGATATATGAAGTCAAGACATTAGATTACCACCAAGCTCTTGAACTCTTCGGTTGGCATGCCTTTGATACTAATGAACCTCCCGTAGAATATGTTGCACTCGCAAGACGTGCAATAGACTATGTTCAACGCCTTCCACGGCTCGAAAGTTTTTGGCACTTACTTGGGTACTCTAGAAACCATAGACCCATCTTATTATGGATTCAATCATGCAATTCGAAACCTAAGTTGGCCATCAAAGAAATCGAAAACATGAAAGATGGGATTGAAGAACTAAGCTTCAAGAACGCTAGAAGGTCTTCAAAGGAGGTGGACGGCAAGGATGCTCACGGCAAGGATGGACAATGGAGGATGAACTTGCTTCACTGCTTCAAAGTCTTGAACAATTGGAGAGGCCGAAAATTTGAGATGGAATGGGCTTTCGTCTTGTCTCCTTTACTTGTTGACGAAAAATTCGGCGGGACCCCGAAACTTTCGAGGTGCAAGGGCTTAAAAGGTGACTTAGTTGAGTCTCCACCGCCAAGCCCTGCTGCCAACTTTCATAGAGGTTTGCAAACGTGCAGCAGGATTGCTATTGCTTTGCAATCCCCGTCGCACCGATGCATGTGTGCTTGTGCGCTACGTTGGCGTATGGGTCAGGTCGTAGAGCCCTATACGCACATGTGTGCAGGGCTAGAGGCCAATAAACGTGCGGGATTGCAACCGACTTACAATCTCGGTCGTCAAGATTCACTTACTTGCAGCGCTGCGGTGGTTCACGGTGAGGCGCTCATCTCATATGGCTTTCGACGCTTGACGGAGCCTCTTACTATCGTGTTCATCCTTCCCGCGTCAGTTGGTATCGGAGCGGGTATCGCCCGCTTCTTAGCAGACGACGATCCAAGGGAGAAGTTCACTACCACCAACCTCAACGACGCTGGTCGTAGAAGTTGGTACATGAAAAGCAATGGGATAGGCAAGAAATGGATGATTGATGAAGCAAGTTTCGGACCGAGTCGAATCAAAGCTGCTGTCCGGTTGGCTTGGGATGACCTAGAAGGTGAAAGGAAGTTGAGAAAGCATTCAGCCGCACTTCCGCACTCACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

427

Amino Acids

48.75

Weight (kDa)

9.56

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 71 5e-08 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 178, 1043
AccII CGCG 1 cut(s) 1025
AclWI GGATC 1 cut(s) 1067
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 2 cut(s) 596, 647
AfaI GTAC 2 cut(s) 365, 1130
AfeI AGCGCT 1 cut(s) 940
AfiI CCNNNNNNNGG 2 cut(s) 112, 172
AflIII ACRYGT 1 cut(s) 855
AgsI TTSAA 9 cut(s) 172, 239, 323, 401, 464, 478, 498, 568, 577
AjiI CACGTC 1 cut(s) 305
AjnI CCWGG 1 cut(s) 105
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AleI CACNNNNGTG 1 cut(s) 858
AluBI AGCT 3 cut(s) 233, 474, 1202
AluI AGCT 3 cut(s) 233, 474, 1202
Alw26I GTCTC 2 cut(s) 630, 711
AlwI GGATC 1 cut(s) 1067
Aor51HI AGCGCT 1 cut(s) 940
AoxI GGCC 3 cut(s) 426, 589, 873
ApeKI GCWGC 5 cut(s) 725, 756, 936, 941, 1202
ApoI RAATTY 2 cut(s) 596, 647
ArsI GACNNNNNNTTYG 4 cut(s) 632, 664, 1191, 1223
Asp700I GAANNNNTTC 2 cut(s) 493, 1255
AspLEI GCGC 3 cut(s) 814, 941, 964
AspS9I GGNCC 2 cut(s) 657, 1184
AsuHPI GGTGA 6 cut(s) 77, 127, 173, 704, 968, 1247
AsuII TTCGAA 1 cut(s) 413
AvaII GGWCC 2 cut(s) 657, 1184
BalI TGGCCA 1 cut(s) 428
BanII GRGCYC 1 cut(s) 846
BbsI GAAGAC 1 cut(s) 486
BbvI GCAGC 5 cut(s) 712, 768, 928, 948, 1189
BccI CCATC 5 cut(s) 392, 437, 449, 527, 598
BceAI ACGGC 3 cut(s) 350, 526, 541
BciT130I CCWGG 1 cut(s) 107
BcoDI GTCTC 2 cut(s) 630, 711
BfaI CTAG 4 cut(s) 369, 486, 869, 1229
BfoI RGCGCY 2 cut(s) 942, 965
BisI GCNGC 7 cut(s) 147, 726, 757, 937, 942, 1203, 1263
BlpI GCTNAGC 1 cut(s) 78
BlsI GCNGC 7 cut(s) 148, 727, 758, 938, 943, 1204, 1264
Bme1390I CCNGG 1 cut(s) 107
Bme18I GGWCC 2 cut(s) 657, 1184
BmgBI CACGTC 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 657, 1184
BmiI GGNNCC 3 cut(s) 658, 659, 996
BmrFI CCNGG 1 cut(s) 107
BmsI GCATC 3 cut(s) 58, 508, 785
BoxI GACNNNNGTC 1 cut(s) 1113
BpiI GAAGAC 1 cut(s) 486
BpmI CTGGAG 1 cut(s) 128
Bpu1102I GCTNAGC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 413
BsaJI CCNNGG 3 cut(s) 72, 332, 1076
BsaWI WCCGGW 1 cut(s) 1209
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 172
Bse1I ACTGG 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 1147
BseBI CCWGG 1 cut(s) 107
BseDI CCNNGG 3 cut(s) 72, 332, 1076
BseGI GGATG 6 cut(s) 523, 538, 552, 1014, 1167, 1228
BseLI CCNNNNNNNGG 2 cut(s) 112, 172
BseMI GCAATG 1 cut(s) 1147
BseNI ACTGG 1 cut(s) 11
BseXI GCAGC 5 cut(s) 712, 768, 928, 948, 1189
BsgI GTGCAG 2 cut(s) 775, 882
Bsh1236I CGCG 1 cut(s) 1025
Bsh1285I CGRYCG 1 cut(s) 916
BshFI GGCC 3 cut(s) 428, 591, 875
BsiEI CGRYCG 1 cut(s) 916
BsiSI CCGG 1 cut(s) 1210
BslFI GGGAC 1 cut(s) 670
BslI CCNNNNNNNGG 2 cut(s) 112, 172
BsmAI GTCTC 2 cut(s) 630, 711
BsmFI GGGAC 1 cut(s) 670
BsmI GAATGC 1 cut(s) 1255
BsnI GGCC 3 cut(s) 428, 591, 875
Bsp119I TTCGAA 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 846
Bsp143I GATC 2 cut(s) 28, 1072
Bsp1720I GCTNAGC 1 cut(s) 78
BspANI GGCC 3 cut(s) 428, 591, 875
BspFNI CGCG 1 cut(s) 1025
BspLI GGNNCC 3 cut(s) 658, 659, 996
BspPI GGATC 1 cut(s) 1067
BspT104I TTCGAA 1 cut(s) 413
BsrBI CCGCTC 2 cut(s) 178, 1043
BsrDI GCAATG 1 cut(s) 1147
BsrI ACTGG 1 cut(s) 11
BssECI CCNNGG 3 cut(s) 72, 332, 1076
BssMI GATC 2 cut(s) 28, 1072
BssT1I CCWWGG 2 cut(s) 72, 1076
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 956
Bst6I CTCTTC 1 cut(s) 248
BstAPI GCANNNNNTGC 1 cut(s) 305
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 2 cut(s) 255, 1054
BstDEI CTNAG 5 cut(s) 78, 420, 470, 697, 1060
BstDSI CCRYGG 1 cut(s) 332
BstEII GGTNACC 1 cut(s) 161
BstENI CCTNNNNNAGG 1 cut(s) 110
BstF5I GGATG 6 cut(s) 523, 538, 552, 1014, 1167, 1228
BstFNI CGCG 1 cut(s) 1025
BstH2I RGCGCY 2 cut(s) 942, 965
BstHHI GCGC 3 cut(s) 814, 941, 964
BstKTI GATC 2 cut(s) 31, 1075
BstMAI GTCTC 2 cut(s) 630, 711
BstMBI GATC 2 cut(s) 28, 1072
BstMCI CGRYCG 1 cut(s) 916
BstMWI GCNNNNNNNGC 5 cut(s) 305, 820, 850, 984, 1049
BstNI CCWGG 1 cut(s) 107
BstNSI RCATGY 3 cut(s) 257, 802, 859
BstPAI GACNNNNGTC 1 cut(s) 1113
BstPI GGTNACC 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 105
BstUI CGCG 1 cut(s) 1025
BstV1I GCAGC 5 cut(s) 712, 768, 928, 948, 1189
BstV2I GAAGAC 1 cut(s) 486
BsuRI GGCC 3 cut(s) 428, 591, 875
BtgI CCRYGG 1 cut(s) 332
BtrI CACGTC 1 cut(s) 305
BtsCI GGATG 6 cut(s) 523, 538, 552, 1014, 1167, 1228
BtsI GCAGTG 1 cut(s) 559
BtsIMutI CAGTG 1 cut(s) 559
Cac8I GCNNGC 2 cut(s) 255, 1054
CfoI GCGC 3 cut(s) 814, 941, 964
Cfr13I GGNCC 2 cut(s) 657, 1184
CpoI CGGWCCG 1 cut(s) 1184
CseI GACGC 3 cut(s) 993, 1014, 1118
Csp6I GTAC 2 cut(s) 364, 1129
CspCI CAANNNNNGTGG 2 cut(s) 486, 521
CspI CGGWCCG 1 cut(s) 1184
CviAII CATG 7 cut(s) 83, 254, 405, 448, 799, 856, 1132
CviQI GTAC 2 cut(s) 364, 1129
DdeI CTNAG 5 cut(s) 78, 420, 470, 697, 1060
DpnI GATC 2 cut(s) 30, 1074
DpnII GATC 2 cut(s) 28, 1072
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Eco130I CCWWGG 2 cut(s) 72, 1076
Eco24I GRGCYC 1 cut(s) 846
Eco47I GGWCC 2 cut(s) 657, 1184
Eco47III AGCGCT 1 cut(s) 940
Eco91I GGTNACC 1 cut(s) 161
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 657
EcoO65I GGTNACC 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 105
EcoT14I CCWWGG 2 cut(s) 72, 1076
EcoT22I ATGCAT 1 cut(s) 800
EcoT38I GRGCYC 1 cut(s) 846
ErhI CCWWGG 2 cut(s) 72, 1076
FaeI CATG 7 cut(s) 86, 257, 408, 451, 802, 859, 1135
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FaqI GGGAC 1 cut(s) 670
FatI CATG 7 cut(s) 82, 253, 404, 447, 798, 855, 1131
FauI CCCGC 6 cut(s) 171, 647, 880, 1030, 1036, 1061
FauNDI CATATG 1 cut(s) 973
Fnu4HI GCNGC 7 cut(s) 147, 726, 757, 937, 942, 1203, 1263
FokI GGATG 6 cut(s) 530, 545, 559, 1001, 1174, 1235
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 7 cut(s) 147, 726, 757, 937, 942, 1203, 1263
FspBI CTAG 4 cut(s) 369, 486, 869, 1229
GlaI GCGC 3 cut(s) 813, 940, 963
GluI GCNGC 7 cut(s) 147, 726, 757, 937, 942, 1203, 1263
GsuI CTGGAG 1 cut(s) 128
HaeII RGCGCY 2 cut(s) 942, 965
HaeIII GGCC 3 cut(s) 428, 591, 875
HapII CCGG 1 cut(s) 1210
HgaI GACGC 3 cut(s) 993, 1014, 1118
HhaI GCGC 3 cut(s) 814, 941, 964
Hin1II CATG 7 cut(s) 86, 257, 408, 451, 802, 859, 1135
Hin6I GCGC 3 cut(s) 812, 939, 962
HinP1I GCGC 3 cut(s) 812, 939, 962
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HindIII AAGCTT 1 cut(s) 472
HinfI GANTC 5 cut(s) 397, 704, 923, 1189, 1194
HpaII CCGG 1 cut(s) 1210
HphI GGTGA 6 cut(s) 77, 127, 173, 704, 968, 1247
Hpy166II GTNNAC 4 cut(s) 508, 640, 952, 1089
Hpy188I TCNGA 3 cut(s) 37, 1040, 1184
Hpy188III TCNNGA 7 cut(s) 211, 236, 369, 478, 574, 920, 1278
Hpy8I GTNNAC 4 cut(s) 508, 640, 952, 1089
Hpy99I CGWCG 4 cut(s) 790, 987, 1073, 1112
HpyAV CCTTC 5 cut(s) 106, 338, 483, 1028, 1226
HpyCH4III ACNGT 1 cut(s) 956
HpyCH4IV ACGT 4 cut(s) 304, 752, 817, 883
HpyF10VI GCNNNNNNNGC 5 cut(s) 305, 820, 850, 984, 1049
HpyF3I CTNAG 5 cut(s) 78, 420, 470, 697, 1060
HpySE526I ACGT 4 cut(s) 304, 752, 817, 883
Hsp92II CATG 7 cut(s) 86, 257, 408, 451, 802, 859, 1135
HspAI GCGC 3 cut(s) 812, 939, 962
KflI GGGWCCC 1 cut(s) 657
Kzo9I GATC 2 cut(s) 28, 1072
LmnI GCTCC 3 cut(s) 175, 994, 1040
Lsp1109I GCAGC 5 cut(s) 712, 768, 928, 948, 1189
LweI GCATC 3 cut(s) 58, 508, 785
MaeI CTAG 4 cut(s) 369, 486, 869, 1229
MaeII ACGT 4 cut(s) 304, 752, 817, 883
MaeIII GTNAC 2 cut(s) 161, 692
MalI GATC 2 cut(s) 30, 1074
MbiI CCGCTC 2 cut(s) 178, 1043
MboI GATC 2 cut(s) 28, 1072
MboII GAAGA 3 cut(s) 235, 476, 486
MfeI CAATTG 1 cut(s) 580
MhlI GDGCHC 1 cut(s) 846
MlsI TGGCCA 1 cut(s) 428
MluCI AATT 6 cut(s) 151, 189, 409, 580, 596, 647
MluNI TGGCCA 1 cut(s) 428
MlyI GAGTC 2 cut(s) 713, 1198
Mox20I TGGCCA 1 cut(s) 428
Mph1103I ATGCAT 1 cut(s) 800
MroXI GAANNNNTTC 2 cut(s) 493, 1255
MscI TGGCCA 1 cut(s) 428
MseI TTAA 3 cut(s) 95, 192, 686
MslI CAYNNNNRTG 1 cut(s) 858
Msp20I TGGCCA 1 cut(s) 428
MspI CCGG 1 cut(s) 1210
MspR9I CCNGG 1 cut(s) 107
MunI CAATTG 1 cut(s) 580
Mva1269I GAATGC 1 cut(s) 1255
MvaI CCWGG 1 cut(s) 107
MvnI CGCG 1 cut(s) 1025
MwoI GCNNNNNNNGC 5 cut(s) 305, 820, 850, 984, 1049
NdeI CATATG 1 cut(s) 973
NdeII GATC 2 cut(s) 28, 1072
NlaIII CATG 7 cut(s) 86, 257, 408, 451, 802, 859, 1135
NlaIV GGNNCC 3 cut(s) 658, 659, 996
NmuCI GTSAC 2 cut(s) 161, 692
NsiI ATGCAT 1 cut(s) 800
NspI RCATGY 3 cut(s) 257, 802, 859
NspV TTCGAA 1 cut(s) 413
OliI CACNNNNGTG 1 cut(s) 858
PaeI GCATGC 1 cut(s) 257
PciI ACATGT 1 cut(s) 855
PctI GAATGC 1 cut(s) 1255
PdmI GAANNNNTTC 2 cut(s) 493, 1255
PfeI GAWTC 3 cut(s) 397, 923, 1194
PflFI GACNNNGTC 1 cut(s) 1188
PkrI GCNGC 7 cut(s) 148, 727, 758, 938, 943, 1204, 1264
PleI GAGTC 2 cut(s) 712, 1197
PpsI GAGTC 2 cut(s) 712, 1197
PpuMI RGGWCCY 1 cut(s) 657
PscI ACATGT 1 cut(s) 855
PshAI GACNNNNGTC 1 cut(s) 1113
Psp5II RGGWCCY 1 cut(s) 657
Psp6I CCWGG 1 cut(s) 105
PspEI GGTNACC 1 cut(s) 161
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 3 cut(s) 658, 659, 996
PspPI GGNCC 2 cut(s) 657, 1184
PspPPI RGGWCCY 1 cut(s) 657
PsyI GACNNNGTC 1 cut(s) 1188
RsaI GTAC 2 cut(s) 365, 1130
RsaNI GTAC 2 cut(s) 364, 1129
RseI CAYNNNNRTG 1 cut(s) 858
Rsr2I CGGWCCG 1 cut(s) 1184
RsrII CGGWCCG 1 cut(s) 1184
SaqAI TTAA 3 cut(s) 95, 192, 686
SatI GCNGC 7 cut(s) 147, 726, 757, 937, 942, 1203, 1263
Sau3AI GATC 2 cut(s) 28, 1072
Sau96I GGNCC 2 cut(s) 657, 1184
SchI GAGTC 2 cut(s) 713, 1198
ScrFI CCNGG 1 cut(s) 107
SduI GDGCHC 1 cut(s) 846
SfaNI GCATC 3 cut(s) 58, 508, 785
SfuI TTCGAA 1 cut(s) 413
SinI GGWCC 2 cut(s) 657, 1184
SmiMI CAYNNNNRTG 1 cut(s) 858
SphI GCATGC 1 cut(s) 257
Sse9I AATT 6 cut(s) 151, 189, 409, 580, 596, 647
SspMI CTAG 4 cut(s) 369, 486, 869, 1229
StyD4I CCNGG 1 cut(s) 105
StyI CCWWGG 2 cut(s) 72, 1076
TaaI ACNGT 1 cut(s) 956
TaiI ACGT 4 cut(s) 307, 755, 820, 886
TaqI TCGA 6 cut(s) 339, 413, 441, 671, 982, 1192
TaqII GACCGA 2 cut(s) 902, 1201
TasI AATT 6 cut(s) 151, 189, 409, 580, 596, 647
TauI GCSGC 2 cut(s) 149, 1265
TfiI GAWTC 3 cut(s) 397, 923, 1194
Tru1I TTAA 3 cut(s) 95, 192, 686
Tru9I TTAA 3 cut(s) 95, 192, 686
TscAI CASTG 1 cut(s) 566
TseFI GTSAC 2 cut(s) 161, 692
TseI GCWGC 5 cut(s) 725, 756, 936, 941, 1202
Tsp45I GTSAC 2 cut(s) 161, 692
TspGWI ACGGA 1 cut(s) 1007
TspRI CASTG 1 cut(s) 566
Tth111I GACNNNGTC 1 cut(s) 1188
VpaK11BI GGWCC 2 cut(s) 657, 1184
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 2 cut(s) 596, 647
XbaI TCTAGA 1 cut(s) 368
XceI RCATGY 3 cut(s) 257, 802, 859
XmnI GAANNNNTTC 2 cut(s) 493, 1255
XspI CTAG 4 cut(s) 369, 486, 869, 1229
Zsp2I ATGCAT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.