Rroxscaffold_7G00206000

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
N/A
Physical Location & Seq
Forward (+)
55649037 .. 55656546
7510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00206000.1

Sequence Viewer

Length: 579 bp
ATGTGTCAACCAATTGCTGGTCGTCACTCATTATTAAGAAACAATGAGACTTGGAAATTAGACTTCAAAGCACCAGATTTTTCCCTTACAGGAATCCACACCTTCGTGGATGATGAACATCTCGGTAGAGGAGAAGAGAAATCGTTTCATGTACTAAAAGCACTTGAAGAGTCAAGGATTTATGTCATCATATTCTCTCGAGATTATGCATCGTCAACATGGTGCTTGGATGAACTTGTCCAGATCCTTCACTGCGGAAAATCAAAGCAACAAAGGGTTATACCAATTTTTTACGAGGTGGATCCCTCAAATGTAAGAAACCAAAGTGGAGAGAGGCGTACGGAGATTGCCGGAGCAGGAGATTGCGCAGAAGGCGATGAGTTTGCCGACGGCGTAGTTGTTGAGGTGGAAGCCGTGGTGATGAAGACGGTGTGGGTGGGTTTGAGTGTGCGCATGTTGGTGCAGCATTGGGCTAAAGACAAGCACCGCTTCCATGCTTGGATTCTCGGTGTAGACCAATTGCTTCATATGCGTCTGCTACGTTCGGGAGTGCGCAATGGAACGGGGTTGTTGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

192

Amino Acids

21.83

Weight (kDa)

6.3

Isoelectric Point (pI)

27.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 25 - 116 4.9e-29 TIR domain
TIR_2 PF13676 30 - 102 2.4e-10 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 367, 452, 554
AccB7I CCANNNNNTGG 1 cut(s) 17
AccI GTMKAC 1 cut(s) 513
AciI CCGC 2 cut(s) 255, 487
AclWI GGATC 3 cut(s) 238, 296, 309
AfaI GTAC 2 cut(s) 153, 340
AfiI CCNNNNNNNGG 1 cut(s) 17
AgsI TTSAA 2 cut(s) 67, 167
AleI CACNNNNGTG 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 3 cut(s) 238, 296, 309
Ama87I CYCGRG 1 cut(s) 198
ApeKI GCWGC 1 cut(s) 463
AspLEI GCGC 3 cut(s) 368, 453, 555
AsuHPI GGTGA 1 cut(s) 430
AvaI CYCGRG 1 cut(s) 198
BamHI GGATCC 1 cut(s) 301
BbsI GAAGAC 1 cut(s) 431
BbvI GCAGC 1 cut(s) 475
BceAI ACGGC 2 cut(s) 398, 406
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BcoDI GTCTC 1 cut(s) 41
BisI GCNGC 1 cut(s) 464
BlsI GCNGC 1 cut(s) 465
BmeT110I CYCGRG 1 cut(s) 198
BmiI GGNNCC 1 cut(s) 303
BmsI GCATC 1 cut(s) 218
BpiI GAAGAC 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 117
BsaJI CCNNGG 1 cut(s) 414
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse3DI GCAATG 1 cut(s) 562
Bse8I GATNNNNATC 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 2 cut(s) 115, 235
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMI GCAATG 1 cut(s) 562
BseRI GAGGAG 1 cut(s) 144
BseXI GCAGC 1 cut(s) 475
BsgI GTGCAG 1 cut(s) 482
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 1 cut(s) 351
BsiWI CGTACG 1 cut(s) 338
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 198
Bsp143I GATC 2 cut(s) 243, 301
BspACI CCGC 2 cut(s) 255, 487
BspLI GGNNCC 1 cut(s) 303
BspPI GGATC 3 cut(s) 238, 296, 309
BsrDI GCAATG 1 cut(s) 562
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 2 cut(s) 243, 301
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 2 cut(s) 129, 162
BstDSI CCRYGG 1 cut(s) 414
BstF5I GGATG 2 cut(s) 115, 235
BstHHI GCGC 3 cut(s) 368, 453, 555
BstKTI GATC 2 cut(s) 246, 304
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 2 cut(s) 243, 301
BstMWI GCNNNNNNNGC 2 cut(s) 372, 529
BstNSI RCATGY 1 cut(s) 457
BstV1I GCAGC 1 cut(s) 475
BstV2I GAAGAC 1 cut(s) 431
BstX2I RGATCY 2 cut(s) 243, 301
BstYI RGATCY 2 cut(s) 243, 301
BtgI CCRYGG 1 cut(s) 414
BtgZI GCGATG 1 cut(s) 390
BtsCI GGATG 2 cut(s) 115, 235
BtsI GCAGTG 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 250
CfoI GCGC 3 cut(s) 368, 453, 555
CseI GACGC 1 cut(s) 521
Csp6I GTAC 2 cut(s) 152, 339
CviAII CATG 4 cut(s) 149, 219, 454, 494
CviJI RGCY 2 cut(s) 413, 473
CviKI_1 RGCY 2 cut(s) 413, 473
CviQI GTAC 2 cut(s) 152, 339
DpnI GATC 2 cut(s) 245, 303
DpnII GATC 2 cut(s) 243, 301
Eam1104I CTCTTC 2 cut(s) 129, 162
EarI CTCTTC 2 cut(s) 129, 162
Eco88I CYCGRG 1 cut(s) 198
EcoT22I ATGCAT 1 cut(s) 211
FaeI CATG 4 cut(s) 152, 222, 457, 497
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FatI CATG 4 cut(s) 148, 218, 453, 493
FauNDI CATATG 1 cut(s) 528
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 1 cut(s) 464
FokI GGATG 2 cut(s) 122, 242
Fsp4HI GCNGC 1 cut(s) 464
FspAI RTGCGCAY 1 cut(s) 452
FspI TGCGCA 3 cut(s) 367, 452, 554
GlaI GCGC 3 cut(s) 367, 452, 554
GluI GCNGC 1 cut(s) 464
HapII CCGG 1 cut(s) 351
HgaI GACGC 1 cut(s) 521
HhaI GCGC 3 cut(s) 368, 453, 555
Hin1II CATG 4 cut(s) 152, 222, 457, 497
Hin6I GCGC 3 cut(s) 366, 451, 553
HinP1I GCGC 3 cut(s) 366, 451, 553
HincII GTYRAC 2 cut(s) 8, 216
HindII GTYRAC 2 cut(s) 8, 216
HinfI GANTC 3 cut(s) 93, 170, 502
HpaII CCGG 1 cut(s) 351
HphI GGTGA 1 cut(s) 430
Hpy166II GTNNAC 3 cut(s) 8, 216, 514
Hpy188III TCNNGA 4 cut(s) 198, 200, 241, 546
Hpy8I GTNNAC 3 cut(s) 8, 216, 514
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 3 cut(s) 112, 257, 365
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 1 cut(s) 541
HpyCH4V TGCA 2 cut(s) 209, 463
HpyF10VI GCNNNNNNNGC 2 cut(s) 372, 529
HpySE526I ACGT 1 cut(s) 541
Hsp92II CATG 4 cut(s) 152, 222, 457, 497
HspAI GCGC 3 cut(s) 366, 451, 553
Kzo9I GATC 2 cut(s) 243, 301
LmnI GCTCC 1 cut(s) 353
LpnPI CCDG 6 cut(s) 3, 75, 87, 254, 342, 364
Lsp1109I GCAGC 1 cut(s) 475
LweI GCATC 1 cut(s) 218
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 1 cut(s) 23
MalI GATC 2 cut(s) 245, 303
MboI GATC 2 cut(s) 243, 301
MboII GAAGA 3 cut(s) 146, 179, 436
MfeI CAATTG 2 cut(s) 12, 518
MflI RGATCY 2 cut(s) 243, 301
MluCI AATT 4 cut(s) 12, 56, 285, 518
MlyI GAGTC 1 cut(s) 179
MnlI CCTC 5 cut(s) 122, 289, 316, 327, 397
Mph1103I ATGCAT 1 cut(s) 211
MseI TTAA 1 cut(s) 35
MslI CAYNNNNRTG 2 cut(s) 104, 458
MspI CCGG 1 cut(s) 351
MunI CAATTG 2 cut(s) 12, 518
MwoI GCNNNNNNNGC 2 cut(s) 372, 529
NdeI CATATG 1 cut(s) 528
NdeII GATC 2 cut(s) 243, 301
NlaIII CATG 4 cut(s) 152, 222, 457, 497
NlaIV GGNNCC 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 23
NsbI TGCGCA 3 cut(s) 367, 452, 554
NsiI ATGCAT 1 cut(s) 211
NspI RCATGY 1 cut(s) 457
OliI CACNNNNGTG 1 cut(s) 104
PaeR7I CTCGAG 1 cut(s) 198
PfeI GAWTC 2 cut(s) 93, 502
Pfl23II CGTACG 1 cut(s) 338
PflMI CCANNNNNTGG 1 cut(s) 17
PkrI GCNGC 1 cut(s) 465
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PspLI CGTACG 1 cut(s) 338
PspN4I GGNNCC 1 cut(s) 303
PsuI RGATCY 2 cut(s) 243, 301
RsaI GTAC 2 cut(s) 153, 340
RsaNI GTAC 2 cut(s) 152, 339
RseI CAYNNNNRTG 2 cut(s) 104, 458
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 464
Sau3AI GATC 2 cut(s) 243, 301
SchI GAGTC 1 cut(s) 179
SetI ASST 4 cut(s) 104, 300, 408, 544
SfaNI GCATC 1 cut(s) 218
Sfr274I CTCGAG 1 cut(s) 198
SlaI CTCGAG 1 cut(s) 198
SmiMI CAYNNNNRTG 2 cut(s) 104, 458
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 4 cut(s) 12, 56, 285, 518
SsiI CCGC 2 cut(s) 255, 487
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 1 cut(s) 544
TaqI TCGA 1 cut(s) 199
TasI AATT 4 cut(s) 12, 56, 285, 518
TatI WGTACW 1 cut(s) 151
TfiI GAWTC 2 cut(s) 93, 502
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 1 cut(s) 257
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 1 cut(s) 463
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 5 cut(s) 129, 137, 246, 437, 515
TspGWI ACGGA 1 cut(s) 356
TspRI CASTG 1 cut(s) 257
Van91I CCANNNNNTGG 1 cut(s) 17
XceI RCATGY 1 cut(s) 457
XhoI CTCGAG 1 cut(s) 198
XmiI GTMKAC 1 cut(s) 513
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.