Rroxscaffold_7G00214950

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
N/A
Physical Location & Seq
Forward (+)
65589191 .. 65590907
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214950.1

Sequence Viewer

Length: 684 bp
ATGTATGATCTAATAGAGAAGGTTGGCCGAGAAAAGGCCCTCCTAGAATCTCTTCATGGTCCTGGAGATCGCAGTAGGTTGTGGACCCATGAAGGAATTCAGTGCCTGACAACTGAAAATAAGAAAAGAGTGGTATGCTTCCGTGCAAAGGCTTTTGATGCAATGGTGAAACTAAGACTGCTCCAAATATATGATAACGGCTCCAATAACTGTAAACAACATGTGATTGGAGACTTCGAGTTTATTTCTCATGAGCTGAGCTTGAGGAGTATGCAATCATTGACGATCGGTGGCATGGAACAACTGCCACTCTCGATGAGAAGCAAACAAACAGAGAAGGAGGGAGTCTCTGTAGTCTCGGGGAGGGAAATTCATCAGGGTCGGGCGAAGGAACTCAAGGGAGAGATGATGGAGGTGGCAGGAGGCAAGGTAGGAGCCTTTGGCAGCTGCTTGGTTTTCTGTAACATCGAAAAAGATAGGAACGGGGAGGCACATAGGAACTCAGATGGGTCTTCTGATTCATATGCAAGTATCAACAGTTTGCAAACAACTGATCAGTTTTTCCAAATTGTTAAAGGAGCTTTCGGAGAATGCATGATTTGGCCAGGACGGAGAATGAATACACCTGTGGAATTGTGCCCCTCATGCTGCCTGGAGACAATTACCGCTGTGTCACTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

227

Amino Acids

25.24

Weight (kDa)

6.99

Isoelectric Point (pI)

50.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 666
AcoI YGGCCR 2 cut(s) 25, 602
AcsI RAATTY 2 cut(s) 96, 369
AfiI CCNNNNNNNGG 2 cut(s) 34, 148
AflIII ACRYGT 1 cut(s) 220
AjnI CCWGG 3 cut(s) 61, 604, 651
AluBI AGCT 4 cut(s) 256, 261, 447, 581
AluI AGCT 4 cut(s) 256, 261, 447, 581
Alw26I GTCTC 4 cut(s) 225, 352, 361, 650
AlwNI CAGNNNCTG 1 cut(s) 106
Ama87I CYCGRG 1 cut(s) 358
AoxI GGCC 3 cut(s) 25, 36, 602
ApeKI GCWGC 3 cut(s) 444, 447, 648
ApoI RAATTY 2 cut(s) 96, 369
Asp700I GAANNNNTTC 2 cut(s) 51, 96
AspS9I GGNCC 3 cut(s) 37, 59, 84
AsuHPI GGTGA 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 2 cut(s) 59, 84
BaeGI GKGCMC 1 cut(s) 641
BalI TGGCCA 1 cut(s) 604
BbsI GAAGAC 1 cut(s) 504
BbvI GCAGC 3 cut(s) 434, 456, 635
BccI CCATC 2 cut(s) 403, 500
BceAI ACGGC 1 cut(s) 214
BciT130I CCWGG 3 cut(s) 63, 606, 653
BclI TGATCA 1 cut(s) 553
BcoDI GTCTC 4 cut(s) 225, 352, 361, 650
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 1 cut(s) 351
BisI GCNGC 3 cut(s) 445, 448, 649
BlpI GCTNAGC 1 cut(s) 257
BlsI GCNGC 3 cut(s) 446, 449, 650
Bme1390I CCNGG 3 cut(s) 63, 606, 653
Bme18I GGWCC 2 cut(s) 59, 84
BmeT110I CYCGRG 1 cut(s) 358
BmgT120I GGNCC 3 cut(s) 37, 59, 84
BmiI GGNNCC 3 cut(s) 86, 202, 436
BmrFI CCNGG 3 cut(s) 63, 606, 653
BmsI GCATC 1 cut(s) 148
BpiI GAAGAC 1 cut(s) 504
BplI GAGNNNNNCTC 2 cut(s) 332, 364
BpmI CTGGAG 2 cut(s) 84, 674
Bpu1102I GCTNAGC 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 283, 380
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 414, 444
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 148
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 3 cut(s) 63, 606, 653
BseLI CCNNNNNNNGG 2 cut(s) 34, 148
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 248, 516
BseRI GAGGAG 1 cut(s) 280
BseSI GKGCMC 1 cut(s) 641
BseXI GCAGC 3 cut(s) 434, 456, 635
Bsh1285I CGRYCG 1 cut(s) 288
BshFI GGCC 3 cut(s) 27, 38, 604
BsiEI CGRYCG 1 cut(s) 288
BsiHKCI CYCGRG 1 cut(s) 358
BslI CCNNNNNNNGG 2 cut(s) 34, 148
BsmAI GTCTC 4 cut(s) 225, 352, 361, 650
BsmI GAATGC 1 cut(s) 596
BsnI GGCC 3 cut(s) 27, 38, 604
BsoBI CYCGRG 1 cut(s) 358
Bsp1286I GDGCHC 1 cut(s) 641
Bsp143I GATC 4 cut(s) 7, 67, 285, 553
Bsp1720I GCTNAGC 1 cut(s) 257
BspACI CCGC 1 cut(s) 666
BspANI GGCC 3 cut(s) 27, 38, 604
BspCNI CTCAG 2 cut(s) 249, 515
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 3 cut(s) 86, 202, 436
BsrDI GCAATG 1 cut(s) 168
BssMI GATC 4 cut(s) 7, 67, 285, 553
Bst2UI CCWGG 3 cut(s) 63, 606, 653
Bst4CI ACNGT 2 cut(s) 212, 539
Bst6I CTCTTC 1 cut(s) 57
BstDEI CTNAG 3 cut(s) 173, 257, 502
BstKTI GATC 4 cut(s) 10, 70, 288, 556
BstMAI GTCTC 4 cut(s) 225, 352, 361, 650
BstMBI GATC 4 cut(s) 7, 67, 285, 553
BstMCI CGRYCG 1 cut(s) 288
BstMWI GCNNNNNNNGC 2 cut(s) 158, 645
BstNI CCWGG 3 cut(s) 63, 606, 653
BstNSI RCATGY 1 cut(s) 224
BstSCI CCNGG 3 cut(s) 61, 604, 651
BstSFI CTRYAG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 641
BstV1I GCAGC 3 cut(s) 434, 456, 635
BstV2I GAAGAC 1 cut(s) 504
BsuRI GGCC 3 cut(s) 27, 38, 604
BtsIMutI CAGTG 1 cut(s) 107
CaiI CAGNNNCTG 1 cut(s) 106
CciI TCATGA 1 cut(s) 250
Cfr13I GGNCC 3 cut(s) 37, 59, 84
CviAII CATG 7 cut(s) 56, 89, 221, 251, 295, 595, 645
DdeI CTNAG 3 cut(s) 173, 257, 502
DpnI GATC 4 cut(s) 9, 69, 287, 555
DpnII GATC 4 cut(s) 7, 67, 285, 553
EaeI YGGCCR 2 cut(s) 25, 602
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Eco47I GGWCC 2 cut(s) 59, 84
Eco88I CYCGRG 1 cut(s) 358
EcoO109I RGGNCCY 1 cut(s) 37
EcoRI GAATTC 1 cut(s) 96
EcoRII CCWGG 3 cut(s) 61, 604, 651
EcoT22I ATGCAT 1 cut(s) 596
FaeI CATG 7 cut(s) 59, 92, 224, 254, 298, 598, 648
FatI CATG 7 cut(s) 55, 88, 220, 250, 294, 594, 644
FauNDI CATATG 1 cut(s) 523
FbaI TGATCA 1 cut(s) 553
Fnu4HI GCNGC 3 cut(s) 445, 448, 649
Fsp4HI GCNGC 3 cut(s) 445, 448, 649
FspBI CTAG 1 cut(s) 44
GluI GCNGC 3 cut(s) 445, 448, 649
GsuI CTGGAG 2 cut(s) 84, 674
HaeIII GGCC 3 cut(s) 27, 38, 604
Hin1II CATG 7 cut(s) 59, 92, 224, 254, 298, 598, 648
HinfI GANTC 3 cut(s) 47, 345, 518
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 2 cut(s) 84, 215
Hpy188I TCNGA 3 cut(s) 505, 517, 587
Hpy188III TCNNGA 2 cut(s) 251, 313
Hpy8I GTNNAC 2 cut(s) 84, 215
HpyAV CCTTC 4 cut(s) 13, 86, 331, 382
HpyCH4III ACNGT 2 cut(s) 212, 539
HpyCH4V TGCA 6 cut(s) 146, 161, 274, 527, 544, 594
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 645
HpyF3I CTNAG 3 cut(s) 173, 257, 502
Hsp92II CATG 7 cut(s) 59, 92, 224, 254, 298, 598, 648
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 4 cut(s) 7, 67, 285, 553
LmnI GCTCC 4 cut(s) 186, 206, 434, 578
Lsp1109I GCAGC 3 cut(s) 434, 456, 635
LweI GCATC 1 cut(s) 148
MaeI CTAG 1 cut(s) 44
MaeIII GTNAC 2 cut(s) 461, 672
MalI GATC 4 cut(s) 9, 69, 287, 555
MboI GATC 4 cut(s) 7, 67, 285, 553
MboII GAAGA 2 cut(s) 44, 504
MhlI GDGCHC 1 cut(s) 641
MlsI TGGCCA 1 cut(s) 604
MluCI AATT 5 cut(s) 96, 369, 567, 632, 660
MluNI TGGCCA 1 cut(s) 604
MlyI GAGTC 1 cut(s) 354
MnlI CCTC 8 cut(s) 50, 258, 334, 357, 406, 416, 481, 652
Mox20I TGGCCA 1 cut(s) 604
Mph1103I ATGCAT 1 cut(s) 596
MroXI GAANNNNTTC 2 cut(s) 51, 96
MscI TGGCCA 1 cut(s) 604
MseI TTAA 2 cut(s) 573, 682
Msp20I TGGCCA 1 cut(s) 604
MspA1I CMGCKG 2 cut(s) 447, 668
MspR9I CCNGG 3 cut(s) 63, 606, 653
Mva1269I GAATGC 1 cut(s) 596
MvaI CCWGG 3 cut(s) 63, 606, 653
MwoI GCNNNNNNNGC 2 cut(s) 158, 645
NdeI CATATG 1 cut(s) 523
NdeII GATC 4 cut(s) 7, 67, 285, 553
NlaIII CATG 7 cut(s) 59, 92, 224, 254, 298, 598, 648
NlaIV GGNNCC 3 cut(s) 86, 202, 436
NmeAIII GCCGAG 1 cut(s) 53
NmuCI GTSAC 1 cut(s) 672
NsiI ATGCAT 1 cut(s) 596
NspI RCATGY 1 cut(s) 224
PagI TCATGA 1 cut(s) 250
PciI ACATGT 1 cut(s) 220
PctI GAATGC 1 cut(s) 596
PdmI GAANNNNTTC 2 cut(s) 51, 96
PfeI GAWTC 2 cut(s) 47, 518
PfoI TCCNGGA 1 cut(s) 61
PkrI GCNGC 3 cut(s) 446, 449, 650
Ple19I CGATCG 1 cut(s) 288
PleI GAGTC 1 cut(s) 353
PpsI GAGTC 1 cut(s) 353
PscI ACATGT 1 cut(s) 220
Psp6I CCWGG 3 cut(s) 61, 604, 651
PspGI CCWGG 3 cut(s) 61, 604, 651
PspN4I GGNNCC 3 cut(s) 86, 202, 436
PspPI GGNCC 3 cut(s) 37, 59, 84
PstNI CAGNNNCTG 1 cut(s) 106
PvuI CGATCG 1 cut(s) 288
PvuII CAGCTG 1 cut(s) 447
SaqAI TTAA 2 cut(s) 573, 682
SatI GCNGC 3 cut(s) 445, 448, 649
Sau3AI GATC 4 cut(s) 7, 67, 285, 553
Sau96I GGNCC 3 cut(s) 37, 59, 84
SchI GAGTC 1 cut(s) 354
ScrFI CCNGG 3 cut(s) 63, 606, 653
SduI GDGCHC 1 cut(s) 641
SetI ASST 9 cut(s) 24, 80, 258, 263, 417, 432, 449, 583, 628
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 351
SinI GGWCC 2 cut(s) 59, 84
SmlI CTYRAG 2 cut(s) 262, 395
SmoI CTYRAG 2 cut(s) 262, 395
Sse9I AATT 5 cut(s) 96, 369, 567, 632, 660
SsiI CCGC 1 cut(s) 666
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 3 cut(s) 61, 604, 651
TaaI ACNGT 2 cut(s) 212, 539
TaqI TCGA 3 cut(s) 237, 314, 468
TasI AATT 5 cut(s) 96, 369, 567, 632, 660
TfiI GAWTC 2 cut(s) 47, 518
Tru1I TTAA 2 cut(s) 573, 682
Tru9I TTAA 2 cut(s) 573, 682
TscAI CASTG 1 cut(s) 107
TseFI GTSAC 1 cut(s) 672
TseI GCWGC 3 cut(s) 444, 447, 648
Tsp45I GTSAC 1 cut(s) 672
TspDTI ATGAA 5 cut(s) 44, 105, 362, 510, 632
TspGWI ACGGA 2 cut(s) 131, 625
TspRI CASTG 1 cut(s) 107
VpaK11BI GGWCC 2 cut(s) 59, 84
XapI RAATTY 2 cut(s) 96, 369
XceI RCATGY 1 cut(s) 224
XmnI GAANNNNTTC 2 cut(s) 51, 96
XspI CTAG 1 cut(s) 44
Zsp2I ATGCAT 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.