Rorug01G0183400

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
N/A
Physical Location & Seq
Reverse (-)
25299608 .. 25299937
330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0183400.1

Sequence Viewer

Length: 330 bp
ATGAAGCCATTGATCCGACGTCTATCCGGCGATGCCGCAACAACTTGGAGACTGGAGTCCCGAATGGACACGTGCTGGTGTATGTCGGCGACGAGATGGAGCGGTTTGTCGTCTCCGCTGAGCTCCTCAACCACCCGGTGTTCGTCGAGCTCCTCAACAAGACGGCGCAAGAGTACGGCTACGAGTAGCGCGGCGTGCTCCAAATTCTCTGCAGCGTGGTGGTGTTTGAGCGTGTCCTCGAGACGCTCCGACGGAAGGCTGTTTCATCTTTCTCTCTCCGCCTGTTATCTCTGGAATCCAAAATGGAAAAAGCTCATAATTTTTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

109

Amino Acids

12.34

Weight (kDa)

11.13

Isoelectric Point (pI)

83.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 22
AccBSI CCGCTC 1 cut(s) 102
AccII CGCG 1 cut(s) 191
AciI CCGC 5 cut(s) 36, 102, 116, 191, 279
AclWI GGATC 1 cut(s) 7
AcsI RAATTY 1 cut(s) 203
AcvI CACGTG 1 cut(s) 72
AcyI GRCGYC 1 cut(s) 19
AdeI CACNNNGTG 1 cut(s) 138
AfaI GTAC 1 cut(s) 175
AfiI CCNNNNNNNGG 1 cut(s) 255
AflIII ACRYGT 1 cut(s) 69
AluBI AGCT 3 cut(s) 123, 150, 313
AluI AGCT 3 cut(s) 123, 150, 313
Alw21I GWGCWC 3 cut(s) 125, 152, 200
Alw26I GTCTC 3 cut(s) 43, 117, 235
AlwI GGATC 1 cut(s) 7
Ama87I CYCGRG 1 cut(s) 238
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 1 cut(s) 203
AspLEI GCGC 2 cut(s) 168, 191
AsuC2I CCSGG 1 cut(s) 136
AvaI CYCGRG 1 cut(s) 238
BanII GRGCYC 2 cut(s) 125, 152
BbrPI CACGTG 1 cut(s) 72
Bbv12I GWGCWC 3 cut(s) 125, 152, 200
BbvI GCAGC 1 cut(s) 224
BccI CCATC 1 cut(s) 90
BceAI ACGGC 2 cut(s) 179, 192
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 3 cut(s) 43, 117, 235
BfmI CTRYAG 1 cut(s) 210
BisI GCNGC 3 cut(s) 36, 192, 213
BlpI GCTNAGC 1 cut(s) 119
BlsI GCNGC 3 cut(s) 37, 193, 214
Bme1390I CCNGG 1 cut(s) 136
BmeT110I CYCGRG 1 cut(s) 238
BmrFI CCNGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 22
BoxI GACNNNNGTC 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 74
Bpu1102I GCTNAGC 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 136
BsaAI YACGTR 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 19
Bsc4I CCNNNNNNNGG 1 cut(s) 255
Bse1I ACTGG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 1 cut(s) 57
BseRI GAGGAG 2 cut(s) 115, 142
BseXI GCAGC 1 cut(s) 224
Bsh1236I CGCG 1 cut(s) 191
BsiHKAI GWGCWC 3 cut(s) 125, 152, 200
BsiHKCI CYCGRG 1 cut(s) 238
BsiSI CCGG 2 cut(s) 27, 136
BslFI GGGAC 1 cut(s) 43
BslI CCNNNNNNNGG 1 cut(s) 255
BsmAI GTCTC 3 cut(s) 43, 117, 235
BsmBI CGTCTC 2 cut(s) 117, 235
BsmFI GGGAC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 238
Bsp1286I GDGCHC 3 cut(s) 125, 152, 200
Bsp143I GATC 1 cut(s) 12
Bsp1720I GCTNAGC 1 cut(s) 119
BspACI CCGC 5 cut(s) 36, 102, 116, 191, 279
BspCNI CTCAG 1 cut(s) 111
BspFNI CGCG 1 cut(s) 191
BspMAI CTGCAG 1 cut(s) 214
BspPI GGATC 1 cut(s) 7
BsrBI CCGCTC 1 cut(s) 102
BsrI ACTGG 1 cut(s) 57
BssMI GATC 1 cut(s) 12
BssNI GRCGYC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 19
BstBAI YACGTR 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 1 cut(s) 119
BstFNI CGCG 1 cut(s) 191
BstHHI GCGC 2 cut(s) 168, 191
BstKTI GATC 1 cut(s) 15
BstMAI GTCTC 3 cut(s) 43, 117, 235
BstMBI GATC 1 cut(s) 12
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstPAI GACNNNNGTC 1 cut(s) 55
BstSCI CCNGG 1 cut(s) 134
BstSFI CTRYAG 1 cut(s) 210
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 224
BtgZI GCGATG 1 cut(s) 45
Cac8I GCNNGC 1 cut(s) 196
CfoI GCGC 2 cut(s) 168, 191
CseI GACGC 1 cut(s) 252
Csp6I GTAC 1 cut(s) 174
CviJI RGCY 6 cut(s) 7, 123, 150, 179, 259, 313
CviKI_1 RGCY 6 cut(s) 7, 123, 150, 179, 259, 313
CviQI GTAC 1 cut(s) 174
DdeI CTNAG 1 cut(s) 119
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
DraIII CACNNNGTG 1 cut(s) 138
EciI GGCGGA 1 cut(s) 268
Ecl136II GAGCTC 2 cut(s) 123, 150
Eco24I GRGCYC 2 cut(s) 125, 152
Eco53kI GAGCTC 2 cut(s) 123, 150
Eco72I CACGTG 1 cut(s) 72
Eco88I CYCGRG 1 cut(s) 238
EcoICRI GAGCTC 2 cut(s) 123, 150
EcoT38I GRGCYC 2 cut(s) 125, 152
Esp3I CGTCTC 2 cut(s) 117, 235
FaiI YATR 2 cut(s) 83, 317
FaqI GGGAC 1 cut(s) 43
Fnu4HI GCNGC 3 cut(s) 36, 192, 213
FriOI GRGCYC 2 cut(s) 125, 152
Fsp4HI GCNGC 3 cut(s) 36, 192, 213
GlaI GCGC 2 cut(s) 167, 190
GluI GCNGC 3 cut(s) 36, 192, 213
GsuI CTGGAG 1 cut(s) 74
HapII CCGG 2 cut(s) 27, 136
HgaI GACGC 1 cut(s) 252
HhaI GCGC 2 cut(s) 168, 191
Hin1I GRCGYC 1 cut(s) 19
Hin6I GCGC 2 cut(s) 166, 189
HinP1I GCGC 2 cut(s) 166, 189
HinfI GANTC 2 cut(s) 56, 295
HpaII CCGG 2 cut(s) 27, 136
Hpy188I TCNGA 2 cut(s) 17, 250
Hpy188III TCNNGA 3 cut(s) 60, 240, 292
Hpy99I CGWCG 4 cut(s) 21, 94, 148, 254
HpyAV CCTTC 1 cut(s) 249
HpyCH4IV ACGT 2 cut(s) 19, 71
HpyCH4V TGCA 1 cut(s) 212
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
HpyF3I CTNAG 1 cut(s) 119
HpySE526I ACGT 2 cut(s) 19, 71
Hsp92I GRCGYC 1 cut(s) 19
HspAI GCGC 2 cut(s) 166, 189
Kzo9I GATC 1 cut(s) 12
LmnI GCTCC 5 cut(s) 99, 128, 155, 203, 251
LpnPI CCDG 6 cut(s) 38, 40, 61, 149, 277, 295
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 1 cut(s) 22
MaeII ACGT 2 cut(s) 19, 71
MalI GATC 1 cut(s) 14
MbiI CCGCTC 1 cut(s) 102
MboI GATC 1 cut(s) 12
MhlI GDGCHC 3 cut(s) 125, 152, 200
MluCI AATT 2 cut(s) 203, 318
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 2 cut(s) 40, 273
MnlI CCTC 3 cut(s) 136, 163, 247
MspA1I CMGCKG 1 cut(s) 118
MspI CCGG 2 cut(s) 27, 136
MspR9I CCNGG 1 cut(s) 136
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 1 cut(s) 195
NciI CCSGG 1 cut(s) 136
NdeII GATC 1 cut(s) 12
PaeR7I CTCGAG 1 cut(s) 238
PfeI GAWTC 1 cut(s) 295
PkrI GCNGC 3 cut(s) 37, 193, 214
PleI GAGTC 1 cut(s) 64
PmaCI CACGTG 1 cut(s) 72
PmlI CACGTG 1 cut(s) 72
PpsI GAGTC 1 cut(s) 64
Ppu21I YACGTR 1 cut(s) 72
PshAI GACNNNNGTC 1 cut(s) 55
Psp124BI GAGCTC 2 cut(s) 125, 152
PspCI CACGTG 1 cut(s) 72
PstI CTGCAG 1 cut(s) 214
RsaI GTAC 1 cut(s) 175
RsaNI GTAC 1 cut(s) 174
SacI GAGCTC 2 cut(s) 125, 152
SatI GCNGC 3 cut(s) 36, 192, 213
Sau3AI GATC 1 cut(s) 12
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 3 cut(s) 125, 152, 200
SetI ASST 5 cut(s) 22, 74, 125, 152, 315
SfaNI GCATC 1 cut(s) 22
SfcI CTRYAG 1 cut(s) 210
Sfr274I CTCGAG 1 cut(s) 238
SlaI CTCGAG 1 cut(s) 238
SmlI CTYRAG 1 cut(s) 238
SmoI CTYRAG 1 cut(s) 238
Sse9I AATT 2 cut(s) 203, 318
SsiI CCGC 5 cut(s) 36, 102, 116, 191, 279
SstI GAGCTC 2 cut(s) 125, 152
StyD4I CCNGG 1 cut(s) 134
TaiI ACGT 2 cut(s) 22, 74
TaqI TCGA 2 cut(s) 146, 239
TasI AATT 2 cut(s) 203, 318
TauI GCSGC 2 cut(s) 38, 194
TfiI GAWTC 1 cut(s) 295
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 2 cut(s) 17, 254
TspGWI ACGGA 1 cut(s) 267
XapI RAATTY 1 cut(s) 203
XhoI CTCGAG 1 cut(s) 238
ZraI GACGTC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.