Rorug01G0247100

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
N/A
Physical Location & Seq
Forward (+)
35457872 .. 35460708
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0247100.1

Sequence Viewer

Length: 984 bp
ATGGAAGGAGAGAAAGGTAGAGTATGTGTAACGGGAGGTTCAGGGTTCATAGGGTCCTGGTTGATTATGAGGCTTCTTGAGCATGGTTACTATGTCAATACCACTGTTAGATCAGACCCAGAACACAAGAAAGATGTAAGCTTCCTCACAGCTCTACCTGGAGCAGAAGAAAGGCTCCAAATTTTCAACGCGGACCTAAGCAACCCGGAAAGTTTCAATGCAGCCATCCAAGGATGCGTCGGAGTGTTCCATGTTGCTACTCCGGTTGACTTCAAAGACAAGGAACCTGAACCAGTAGTGACCAAACGATCAGTCGATGGAGCCCTTGGTATCCTCAAGGCGTGCCTATATGCAAAAACAGTGAAACGAGTTGTGTACACTTCTAGTTTATCTGCTGTTCAATTTAACAACAAGGATGTGGAAGACATGGATGAGAGTTTTTGGAGTGACACAGATTACATTAAAGCTCTAAAGCCATATGGAGGATCATACGTGATTTCGAAGACATTGACTGAAAGAGCAGTTCTTGAATTTTCGGAGAAAAATGGATTGGATGTTGTGACAGTAATTCCTTCTTTTGTTGTTGGCCCCTTCATTTGTCCCAAGTTCCCCAGCTCTATTCGATCCGCATTGGCTCTGGTTTTAGGTAACAGGGATGAATATCCTATTCTTATCAATTTATCAGTGGTACATGTGGATGATGTCGCCAGAGCACACATTTTCCTTTTTGAACATCATGATGCAAAAGGGAGATACAATTGCTCATCAAATGTCATAACAATTGAAGAAATGGCCAAGTTCCTTTCTGCCAAATTCCCCGAATTTCAAATACCCTCAACAGAGTCATTGAAGGAAGTTAAAGGTCCTAAGATTCCTGGTCTGTCATCAAAGAAGCTCTTGGATACGGGTTTCAAATTTAAGTATGGGGTTGATGAGATGTTCAGTGAGGCAATTCACTGCTGCAAAGAGAATGGTTATCTGTAA

Protein Analysis

327

Amino Acids

36.21

Weight (kDa)

5.95

Isoelectric Point (pI)

29.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 138 3.4e-08 NmrA-like family
Epimerase PF01370 8 - 254 1.2e-25 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 241 2.5e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 267 7.6e-16 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 196 3.2e-13 Male sterility protein
NAD_binding_10 PF13460 12 - 147 1e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 191
AciI CCGC 2 cut(s) 191, 627
AclWI GGATC 2 cut(s) 493, 618
AcoI YGGCCR 1 cut(s) 792
AcsI RAATTY 5 cut(s) 180, 530, 812, 821, 914
AfaI GTAC 2 cut(s) 377, 690
AfiI CCNNNNNNNGG 1 cut(s) 482
AflIII ACRYGT 1 cut(s) 691
AjnI CCWGG 3 cut(s) 56, 157, 874
AjuI GAANNNNNNNTTGG 2 cut(s) 533, 565
AluBI AGCT 5 cut(s) 141, 152, 467, 615, 895
AluI AGCT 5 cut(s) 141, 152, 467, 615, 895
Alw21I GWGCWC 1 cut(s) 715
AlwI GGATC 2 cut(s) 493, 618
AoxI GGCC 2 cut(s) 586, 792
ApeKI GCWGC 2 cut(s) 221, 960
ApoI RAATTY 5 cut(s) 180, 530, 812, 821, 914
ArsI GACNNNNNNTTYG 2 cut(s) 297, 329
AspS9I GGNCC 4 cut(s) 54, 193, 587, 863
AsuC2I CCSGG 1 cut(s) 206
AsuII TTCGAA 1 cut(s) 500
AvaII GGWCC 3 cut(s) 54, 193, 863
BalI TGGCCA 1 cut(s) 794
BanII GRGCYC 1 cut(s) 325
BbsI GAAGAC 2 cut(s) 429, 509
Bbv12I GWGCWC 1 cut(s) 715
BbvI GCAGC 2 cut(s) 233, 947
BccI CCATC 2 cut(s) 233, 311
BciT130I CCWGG 3 cut(s) 58, 159, 876
BciVI GTATCC 2 cut(s) 341, 895
BcnI CCSGG 1 cut(s) 206
BfaI CTAG 1 cut(s) 384
BfuI GTATCC 2 cut(s) 341, 895
BisI GCNGC 2 cut(s) 222, 961
BlsI GCNGC 2 cut(s) 223, 962
Bme1390I CCNGG 4 cut(s) 58, 159, 206, 876
Bme18I GGWCC 3 cut(s) 54, 193, 863
BmgT120I GGNCC 4 cut(s) 54, 193, 587, 863
BmiI GGNNCC 5 cut(s) 55, 176, 285, 322, 589
BmrFI CCNGG 4 cut(s) 58, 159, 206, 876
BmsI GCATC 2 cut(s) 224, 730
BpiI GAAGAC 2 cut(s) 429, 509
BpmI CTGGAG 1 cut(s) 180
Bpu10I CCTNAGC 1 cut(s) 197
Bpu14I TTCGAA 1 cut(s) 500
BpuEI CTTGAG 2 cut(s) 98, 320
BpuMI CCSGG 1 cut(s) 206
BsaAI YACGTR 1 cut(s) 493
BsaBI GATNNNNATC 1 cut(s) 660
BsaJI CCNNGG 2 cut(s) 229, 325
BsaWI WCCGGW 1 cut(s) 262
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse1I ACTGG 1 cut(s) 293
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 3 cut(s) 58, 159, 876
BseDI CCNNGG 2 cut(s) 229, 325
BseGI GGATG 7 cut(s) 225, 239, 421, 436, 559, 661, 703
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 1 cut(s) 482
BseNI ACTGG 1 cut(s) 293
BseXI GCAGC 2 cut(s) 233, 947
BseYI CCCAGC 1 cut(s) 611
Bsh1236I CGCG 1 cut(s) 191
BshFI GGCC 2 cut(s) 588, 794
BsiHKAI GWGCWC 1 cut(s) 715
BsiSI CCGG 2 cut(s) 206, 263
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 585
BsnI GGCC 2 cut(s) 588, 794
Bsp119I TTCGAA 1 cut(s) 500
Bsp1286I GDGCHC 2 cut(s) 325, 715
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 4 cut(s) 110, 308, 485, 623
BspACI CCGC 2 cut(s) 191, 627
BspANI GGCC 2 cut(s) 588, 794
BspFNI CGCG 1 cut(s) 191
BspHI TCATGA 1 cut(s) 736
BspLI GGNNCC 5 cut(s) 55, 176, 285, 322, 589
BspPI GGATC 2 cut(s) 493, 618
BspT104I TTCGAA 1 cut(s) 500
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 1 cut(s) 293
BssECI CCNNGG 2 cut(s) 229, 325
BssMI GATC 4 cut(s) 110, 308, 485, 623
BssT1I CCWWGG 2 cut(s) 229, 325
Bst2UI CCWGG 3 cut(s) 58, 159, 876
Bst4CI ACNGT 3 cut(s) 106, 361, 565
BstAUI TGTACA 1 cut(s) 375
BstBAI YACGTR 1 cut(s) 493
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 2 cut(s) 197, 867
BstF5I GGATG 7 cut(s) 225, 239, 421, 436, 559, 661, 703
BstFNI CGCG 1 cut(s) 191
BstKTI GATC 4 cut(s) 113, 311, 488, 626
BstMBI GATC 4 cut(s) 110, 308, 485, 623
BstMWI GCNNNNNNNGC 1 cut(s) 79
BstNI CCWGG 3 cut(s) 58, 159, 876
BstNSI RCATGY 1 cut(s) 695
BstSCI CCNGG 4 cut(s) 56, 157, 204, 874
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 2 cut(s) 233, 947
BstV2I GAAGAC 2 cut(s) 429, 509
BsuI GTATCC 2 cut(s) 341, 895
BsuRI GGCC 2 cut(s) 588, 794
BtsCI GGATG 7 cut(s) 225, 239, 421, 436, 559, 661, 703
BtsI GCAGTG 1 cut(s) 955
BtsIMutI CAGTG 5 cut(s) 102, 366, 690, 949, 955
Cac8I GCNNGC 1 cut(s) 343
CciI TCATGA 1 cut(s) 736
Cfr13I GGNCC 4 cut(s) 54, 193, 587, 863
CseI GACGC 1 cut(s) 226
Csp6I GTAC 2 cut(s) 376, 689
CviAII CATG 5 cut(s) 83, 251, 427, 692, 737
CviQI GTAC 2 cut(s) 376, 689
DdeI CTNAG 2 cut(s) 197, 867
DpnI GATC 4 cut(s) 112, 310, 487, 625
DpnII GATC 4 cut(s) 110, 308, 485, 623
EaeI YGGCCR 1 cut(s) 792
Eco130I CCWWGG 2 cut(s) 229, 325
Eco24I GRGCYC 1 cut(s) 325
Eco47I GGWCC 3 cut(s) 54, 193, 863
EcoO109I RGGNCCY 2 cut(s) 54, 863
EcoRII CCWGG 3 cut(s) 56, 157, 874
EcoT14I CCWWGG 2 cut(s) 229, 325
EcoT38I GRGCYC 1 cut(s) 325
ErhI CCWWGG 2 cut(s) 229, 325
FaeI CATG 5 cut(s) 86, 254, 430, 695, 740
FalI AAGNNNNNCTT 2 cut(s) 881, 913
FaqI GGGAC 1 cut(s) 585
FatI CATG 5 cut(s) 82, 250, 426, 691, 736
FauNDI CATATG 1 cut(s) 478
Fnu4HI GCNGC 2 cut(s) 222, 961
FokI GGATG 7 cut(s) 212, 246, 428, 443, 566, 668, 710
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 2 cut(s) 222, 961
FspBI CTAG 1 cut(s) 384
GluI GCNGC 2 cut(s) 222, 961
GsaI CCCAGC 1 cut(s) 615
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 2 cut(s) 588, 794
HapII CCGG 2 cut(s) 206, 263
HgaI GACGC 1 cut(s) 226
Hin1II CATG 5 cut(s) 86, 254, 430, 695, 740
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 139
HinfI GANTC 2 cut(s) 842, 871
HpaII CCGG 2 cut(s) 206, 263
Hpy166II GTNNAC 3 cut(s) 268, 376, 378
Hpy188I TCNGA 3 cut(s) 115, 242, 538
Hpy188III TCNNGA 3 cut(s) 77, 527, 737
Hpy8I GTNNAC 3 cut(s) 268, 376, 378
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 3 cut(s) 582, 601, 844
HpyCH4III ACNGT 3 cut(s) 106, 361, 565
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 4 cut(s) 221, 353, 743, 963
HpyF10VI GCNNNNNNNGC 1 cut(s) 79
HpyF3I CTNAG 2 cut(s) 197, 867
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 5 cut(s) 86, 254, 430, 695, 740
Kzo9I GATC 4 cut(s) 110, 308, 485, 623
LmnI GCTCC 3 cut(s) 161, 180, 320
Lsp1109I GCAGC 2 cut(s) 233, 947
LweI GCATC 2 cut(s) 224, 730
MaeI CTAG 1 cut(s) 384
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 6 cut(s) 28, 86, 298, 446, 559, 647
MalI GATC 4 cut(s) 112, 310, 487, 625
MboI GATC 4 cut(s) 110, 308, 485, 623
MboII GAAGA 4 cut(s) 179, 434, 514, 797
MfeI CAATTG 2 cut(s) 757, 780
MhlI GDGCHC 2 cut(s) 325, 715
MlsI TGGCCA 1 cut(s) 794
MluNI TGGCCA 1 cut(s) 794
MlyI GAGTC 1 cut(s) 851
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 7 cut(s) 29, 63, 155, 344, 476, 844, 940
Mox20I TGGCCA 1 cut(s) 794
MscI TGGCCA 1 cut(s) 794
MseI TTAA 4 cut(s) 405, 462, 858, 918
MslI CAYNNNNRTG 2 cut(s) 696, 738
Msp20I TGGCCA 1 cut(s) 794
MspI CCGG 2 cut(s) 206, 263
MspR9I CCNGG 4 cut(s) 58, 159, 206, 876
MunI CAATTG 2 cut(s) 757, 780
MvaI CCWGG 3 cut(s) 58, 159, 876
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 1 cut(s) 79
NciI CCSGG 1 cut(s) 206
NdeI CATATG 1 cut(s) 478
NdeII GATC 4 cut(s) 110, 308, 485, 623
NlaIII CATG 5 cut(s) 86, 254, 430, 695, 740
NlaIV GGNNCC 5 cut(s) 55, 176, 285, 322, 589
NmuCI GTSAC 3 cut(s) 298, 446, 559
NspI RCATGY 1 cut(s) 695
NspV TTCGAA 1 cut(s) 500
PagI TCATGA 1 cut(s) 736
PciI ACATGT 1 cut(s) 691
PfeI GAWTC 1 cut(s) 871
PkrI GCNGC 2 cut(s) 223, 962
PleI GAGTC 1 cut(s) 850
PpsI GAGTC 1 cut(s) 850
Ppu21I YACGTR 1 cut(s) 493
PpuMI RGGWCCY 2 cut(s) 54, 863
PscI ACATGT 1 cut(s) 691
Psp5II RGGWCCY 2 cut(s) 54, 863
Psp6I CCWGG 3 cut(s) 56, 157, 874
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 3 cut(s) 56, 157, 874
PspN4I GGNNCC 5 cut(s) 55, 176, 285, 322, 589
PspPI GGNCC 4 cut(s) 54, 193, 587, 863
PspPPI RGGWCCY 2 cut(s) 54, 863
RsaI GTAC 2 cut(s) 377, 690
RsaNI GTAC 2 cut(s) 376, 689
RseI CAYNNNNRTG 2 cut(s) 696, 738
SaqAI TTAA 4 cut(s) 405, 462, 858, 918
SatI GCNGC 2 cut(s) 222, 961
Sau3AI GATC 4 cut(s) 110, 308, 485, 623
Sau96I GGNCC 4 cut(s) 54, 193, 587, 863
SchI GAGTC 1 cut(s) 851
ScrFI CCNGG 4 cut(s) 58, 159, 206, 876
SduI GDGCHC 2 cut(s) 325, 715
SfaNI GCATC 2 cut(s) 224, 730
SfuI TTCGAA 1 cut(s) 500
SinI GGWCC 3 cut(s) 54, 193, 863
SmiMI CAYNNNNRTG 2 cut(s) 696, 738
SmlI CTYRAG 2 cut(s) 77, 335
SmoI CTYRAG 2 cut(s) 77, 335
SsiI CCGC 2 cut(s) 191, 627
SspMI CTAG 1 cut(s) 384
StyD4I CCNGG 4 cut(s) 56, 157, 204, 874
StyI CCWWGG 2 cut(s) 229, 325
TaaI ACNGT 3 cut(s) 106, 361, 565
TaiI ACGT 1 cut(s) 495
TaqI TCGA 3 cut(s) 315, 500, 622
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 1 cut(s) 871
Tru1I TTAA 4 cut(s) 405, 462, 858, 918
Tru9I TTAA 4 cut(s) 405, 462, 858, 918
TscAI CASTG 5 cut(s) 109, 366, 690, 949, 962
TseFI GTSAC 3 cut(s) 298, 446, 559
TseI GCWGC 2 cut(s) 221, 960
Tsp45I GTSAC 3 cut(s) 298, 446, 559
TspDTI ATGAA 3 cut(s) 37, 583, 672
TspRI CASTG 5 cut(s) 109, 366, 690, 949, 962
VpaK11BI GGWCC 3 cut(s) 54, 193, 863
XapI RAATTY 5 cut(s) 180, 530, 812, 821, 914
XceI RCATGY 1 cut(s) 695
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.