Rorug01G0249800
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
N/A
Physical Location & Seq
Forward (+)
35817929 .. 35819589
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0249800.1

Sequence Viewer

Length: 966 bp
ATGGATCCGGTTGCAGCACACGGCCGACCACTTCCGCCTCCGTTTTTGACGAGAGATCTTCATCTAAATCACCCTCATCATCAGTTCCAACACCACCCTCATCTCCTCCACCACCAGAATTCTGAAGACGAGCAGAACAGCTCCGGCTTAAACCACCACCGCGGAATCAAGCGCGACCGGGACGAGAACATCTCCGGCGCCACCACCACGAACTCGCTGGAAGGCAAGGACCAGCTTGGTGGGTCCACTAGCGGAGAAGGCGGCGAGATCACGAGACGACCTCGTGGCCGACCCGCTGGCTCCAAGAACAAGGCCAAACCCCCAATCATCATCACGCGCGACAGCGCCAACGCGTTGCGGTCTCACGTCATGGAGGTCGCCAACGGCTGCGACATCATGGACTCCATCTCCACCTTCGCCCGCCGGAGACAAAGAGGCGTTTGCATTCTCAGCGGCAGCGGCACCGTCACCAATGTAACCCTCCGCCAGCCAGCTTCGCCCGGCGCCGTGGTCACCCTCCACGGTAGGTTCGAAATTCTTTCCCTTTCGGGATCATTCCTGCCCCCACCTGCTCCGCCCGCGGCGTCAGGACTCACCATTTACCTGGCTGGCGGGCAGGGACAGGTAGTCGGAGGCAGCGTCGTGGGCCCGCTCCTAGCGTCCGGCCCTGTGGTCATTATGGCAGCGAGCTTCGGTAATGCTGCCTACGAGAGACTGCCCCTGGAGGAGGAGGAGGCCACGGCGGTGACGCCTATGCCTGGAAGCGGGCCCCTTGGCTCCCCCGGAATTGGCGGAGGAGAGCACCAGCAACAGTCACAAGTTCAACAAGTACAGCAGCAGATGTTGCAAGATGCTAATAACTCATCACCTTCTTCCTCACTCTTTCATGGGATGCAGCAAAATCTTCTAAATTCGGTTCAATTGCCCGCTGAGGCATATTGGGGCACAGCTCGCCCTCCTTATTGA

Protein Analysis

321

Amino Acids

33.95

Weight (kDa)

6.98

Isoelectric Point (pI)

53.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 120 - 231 6.4e-25 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 577
Acc36I ACCTGC 1 cut(s) 577
AccB1I GGYRCC 3 cut(s) 197, 461, 503
AccBSI CCGCTC 1 cut(s) 652
AccII CGCG 6 cut(s) 162, 174, 337, 339, 353, 581
AclWI GGATC 2 cut(s) 12, 559
AcoI YGGCCR 2 cut(s) 22, 286
AcsI RAATTY 3 cut(s) 118, 534, 910
AcuI CTGAAG 1 cut(s) 144
AcyI GRCGYC 4 cut(s) 198, 504, 584, 749
AfaI GTAC 1 cut(s) 831
AfiI CCNNNNNNNGG 4 cut(s) 727, 758, 764, 788
AflIII ACRYGT 1 cut(s) 351
AgsI TTSAA 2 cut(s) 824, 920
AhdI GACNNNNNGTC 1 cut(s) 626
AjiI CACGTC 1 cut(s) 367
AjnI CCWGG 3 cut(s) 603, 720, 757
AleI CACNNNNGTG 1 cut(s) 743
AluBI AGCT 5 cut(s) 141, 235, 494, 690, 950
AluI AGCT 5 cut(s) 141, 235, 494, 690, 950
Alw21I GWGCWC 1 cut(s) 804
Alw26I GTCTC 4 cut(s) 268, 366, 421, 706
AlwI GGATC 2 cut(s) 12, 559
AoxI GGCC 7 cut(s) 22, 286, 312, 646, 664, 735, 767
ApaI GGGCCC 2 cut(s) 650, 771
ApeKI GCWGC 8 cut(s) 14, 387, 456, 636, 683, 701, 835, 895
ApoI RAATTY 3 cut(s) 118, 534, 910
AspLEI GCGC 5 cut(s) 174, 200, 339, 347, 506
AspS9I GGNCC 7 cut(s) 229, 243, 646, 647, 665, 767, 768
AsuC2I CCSGG 3 cut(s) 179, 501, 783
AsuHPI GGTGA 6 cut(s) 62, 460, 505, 586, 757, 858
AsuII TTCGAA 1 cut(s) 531
AvaII GGWCC 2 cut(s) 229, 243
BaeGI GKGCMC 3 cut(s) 650, 771, 947
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 3 cut(s) 197, 461, 503
BanII GRGCYC 2 cut(s) 650, 771
BauI CACGAG 2 cut(s) 271, 282
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 804
BbvCI CCTCAGC 1 cut(s) 930
BbvI GCAGC 8 cut(s) 26, 374, 468, 648, 688, 695, 847, 907
BccI CCATC 1 cut(s) 413
BceAI ACGGC 4 cut(s) 37, 400, 491, 756
BciT130I CCWGG 3 cut(s) 605, 722, 759
BcnI CCSGG 3 cut(s) 179, 501, 783
BcoDI GTCTC 4 cut(s) 268, 366, 421, 706
BfaI CTAG 2 cut(s) 249, 656
BfoI RGCGCY 3 cut(s) 201, 348, 507
BfuAI ACCTGC 1 cut(s) 577
BglII AGATCT 1 cut(s) 55
Bme1390I CCNGG 6 cut(s) 179, 501, 605, 722, 759, 783
Bme18I GGWCC 2 cut(s) 229, 243
BmeRI GACNNNNNGTC 1 cut(s) 626
BmgBI CACGTC 1 cut(s) 367
BmgT120I GGNCC 7 cut(s) 229, 243, 646, 647, 665, 767, 768
BmrFI CCNGG 6 cut(s) 179, 501, 605, 722, 759, 783
BmsI GCATC 2 cut(s) 841, 882
BpiI GAAGAC 1 cut(s) 132
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 265, 297
BpmI CTGGAG 1 cut(s) 743
Bpu10I CCTNAGC 1 cut(s) 930
Bpu14I TTCGAA 1 cut(s) 531
BpuMI CCSGG 3 cut(s) 179, 501, 783
BsaBI GATNNNNATC 1 cut(s) 60
BsaHI GRCGYC 4 cut(s) 198, 504, 584, 749
BsaI GGTCTC 1 cut(s) 366
BsaJI CCNNGG 8 cut(s) 160, 507, 520, 579, 720, 738, 772, 781
BsaWI WCCGGW 1 cut(s) 7
BsaXI ACNNNNNCTCC 4 cut(s) 392, 422, 624, 654
Bsc4I CCNNNNNNNGG 4 cut(s) 727, 758, 764, 788
Bse8I GATNNNNATC 1 cut(s) 60
BseBI CCWGG 3 cut(s) 605, 722, 759
BseDI CCNNGG 8 cut(s) 160, 507, 520, 579, 720, 738, 772, 781
BseGI GGATG 1 cut(s) 897
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 4 cut(s) 727, 758, 764, 788
BseMII CTCAG 2 cut(s) 463, 921
BseRI GAGGAG 5 cut(s) 95, 740, 743, 746, 810
BseSI GKGCMC 3 cut(s) 650, 771, 947
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 8 cut(s) 26, 374, 468, 648, 688, 695, 847, 907
Bsh1236I CGCG 6 cut(s) 162, 174, 337, 339, 353, 581
Bsh1285I CGRYCG 2 cut(s) 25, 178
BshFI GGCC 7 cut(s) 24, 288, 314, 648, 666, 737, 769
BshNI GGYRCC 3 cut(s) 197, 461, 503
BsiEI CGRYCG 2 cut(s) 25, 178
BsiHKAI GWGCWC 1 cut(s) 804
BsiSI CCGG 8 cut(s) 8, 144, 178, 195, 424, 501, 663, 783
BslFI GGGAC 2 cut(s) 194, 633
BslI CCNNNNNNNGG 4 cut(s) 727, 758, 764, 788
BsmAI GTCTC 4 cut(s) 268, 366, 421, 706
BsmBI CGTCTC 1 cut(s) 268
BsmFI GGGAC 2 cut(s) 194, 633
BsmI GAATGC 1 cut(s) 444
BsnI GGCC 7 cut(s) 24, 288, 314, 648, 666, 737, 769
Bso31I GGTCTC 1 cut(s) 366
Bsp119I TTCGAA 1 cut(s) 531
Bsp120I GGGCCC 2 cut(s) 646, 767
Bsp1286I GDGCHC 4 cut(s) 650, 771, 804, 947
Bsp143I GATC 4 cut(s) 4, 55, 267, 551
BspANI GGCC 7 cut(s) 24, 288, 314, 648, 666, 737, 769
BspCNI CTCAG 2 cut(s) 462, 922
BspFNI CGCG 6 cut(s) 162, 174, 337, 339, 353, 581
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 2 cut(s) 12, 559
BspT104I TTCGAA 1 cut(s) 531
BspT107I GGYRCC 3 cut(s) 197, 461, 503
BspTNI GGTCTC 1 cut(s) 366
BsrBI CCGCTC 1 cut(s) 652
BssECI CCNNGG 8 cut(s) 160, 507, 520, 579, 720, 738, 772, 781
BssMI GATC 4 cut(s) 4, 55, 267, 551
BssNI GRCGYC 4 cut(s) 198, 504, 584, 749
BssSI CACGAG 2 cut(s) 271, 282
BssT1I CCWWGG 1 cut(s) 772
Bst2BI CACGAG 2 cut(s) 271, 282
Bst2UI CCWGG 3 cut(s) 605, 722, 759
Bst4CI ACNGT 3 cut(s) 466, 524, 813
BstACI GRCGYC 4 cut(s) 198, 504, 584, 749
BstAPI GCANNNNNTGC 1 cut(s) 844
BstBI TTCGAA 1 cut(s) 531
BstDEI CTNAG 2 cut(s) 449, 930
BstDSI CCRYGG 5 cut(s) 160, 507, 520, 579, 738
BstEII GGTNACC 1 cut(s) 511
BstENI CCTNNNNNAGG 1 cut(s) 725
BstF5I GGATG 1 cut(s) 897
BstFNI CGCG 6 cut(s) 162, 174, 337, 339, 353, 581
BstH2I RGCGCY 3 cut(s) 201, 348, 507
BstHHI GCGC 5 cut(s) 174, 200, 339, 347, 506
BstKTI GATC 4 cut(s) 7, 58, 270, 554
BstMAI GTCTC 4 cut(s) 268, 366, 421, 706
BstMBI GATC 4 cut(s) 4, 55, 267, 551
BstMCI CGRYCG 2 cut(s) 25, 178
BstMWI GCNNNNNNNGC 8 cut(s) 258, 450, 459, 496, 578, 645, 844, 951
BstNI CCWGG 3 cut(s) 605, 722, 759
BstPI GGTNACC 1 cut(s) 511
BstSCI CCNGG 6 cut(s) 177, 499, 603, 720, 757, 781
BstSLI GKGCMC 3 cut(s) 650, 771, 947
BstUI CGCG 6 cut(s) 162, 174, 337, 339, 353, 581
BstV1I GCAGC 8 cut(s) 26, 374, 468, 648, 688, 695, 847, 907
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 2 cut(s) 4, 55
BstXI CCANNNNNNTGG 2 cut(s) 239, 604
BstYI RGATCY 2 cut(s) 4, 55
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 7 cut(s) 24, 288, 314, 648, 666, 737, 769
BtgI CCRYGG 5 cut(s) 160, 507, 520, 579, 738
BtrI CACGTC 1 cut(s) 367
BtsCI GGATG 1 cut(s) 897
BveI ACCTGC 1 cut(s) 577
CfoI GCGC 5 cut(s) 174, 200, 339, 347, 506
Cfr13I GGNCC 7 cut(s) 229, 243, 646, 647, 665, 767, 768
Cfr42I CCGCGG 2 cut(s) 163, 582
CseI GACGC 4 cut(s) 573, 628, 648, 757
Csp6I GTAC 1 cut(s) 830
CviAII CATG 3 cut(s) 370, 397, 887
CviQI GTAC 1 cut(s) 830
DdeI CTNAG 2 cut(s) 449, 930
DinI GGCGCC 2 cut(s) 199, 505
DpnI GATC 4 cut(s) 6, 57, 269, 553
DpnII GATC 4 cut(s) 4, 55, 267, 551
DriI GACNNNNNGTC 1 cut(s) 626
EaeI YGGCCR 2 cut(s) 22, 286
EagI CGGCCG 1 cut(s) 22
Eam1105I GACNNNNNGTC 1 cut(s) 626
EciI GGCGGA 4 cut(s) 24, 473, 564, 807
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 772
Eco24I GRGCYC 2 cut(s) 650, 771
Eco31I GGTCTC 1 cut(s) 366
Eco47I GGWCC 2 cut(s) 229, 243
Eco52I CGGCCG 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 144
Eco91I GGTNACC 1 cut(s) 511
EcoNI CCTNNNNNAGG 1 cut(s) 725
EcoO109I RGGNCCY 1 cut(s) 768
EcoO65I GGTNACC 1 cut(s) 511
EcoRI GAATTC 1 cut(s) 118
EcoRII CCWGG 3 cut(s) 603, 720, 757
EcoT14I CCWWGG 1 cut(s) 772
EcoT38I GRGCYC 2 cut(s) 650, 771
EgeI GGCGCC 2 cut(s) 199, 505
EheI GGCGCC 2 cut(s) 199, 505
ErhI CCWWGG 1 cut(s) 772
Esp3I CGTCTC 1 cut(s) 268
FaeI CATG 3 cut(s) 373, 400, 890
FaiI YATR 6 cut(s) 371, 398, 680, 755, 888, 937
FaqI GGGAC 2 cut(s) 194, 633
FatI CATG 3 cut(s) 369, 396, 886
FauI CCCGC 7 cut(s) 301, 428, 586, 605, 657, 758, 934
FokI GGATG 1 cut(s) 904
FriOI GRGCYC 2 cut(s) 650, 771
FspBI CTAG 2 cut(s) 249, 656
GlaI GCGC 5 cut(s) 173, 199, 338, 346, 505
GsuI CTGGAG 1 cut(s) 743
HaeII RGCGCY 3 cut(s) 201, 348, 507
HaeIII GGCC 7 cut(s) 24, 288, 314, 648, 666, 737, 769
HapII CCGG 8 cut(s) 8, 144, 178, 195, 424, 501, 663, 783
HgaI GACGC 4 cut(s) 573, 628, 648, 757
HhaI GCGC 5 cut(s) 174, 200, 339, 347, 506
Hin1I GRCGYC 4 cut(s) 198, 504, 584, 749
Hin1II CATG 3 cut(s) 373, 400, 890
Hin6I GCGC 5 cut(s) 172, 198, 337, 345, 504
HinP1I GCGC 5 cut(s) 172, 198, 337, 345, 504
HinfI GANTC 3 cut(s) 165, 401, 591
HpaII CCGG 8 cut(s) 8, 144, 178, 195, 424, 501, 663, 783
HphI GGTGA 6 cut(s) 62, 460, 505, 586, 757, 858
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 2 cut(s) 124, 632
Hpy188III TCNNGA 3 cut(s) 271, 549, 588
Hpy8I GTNNAC 1 cut(s) 246
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 4 cut(s) 215, 251, 424, 879
HpyCH4III ACNGT 3 cut(s) 466, 524, 813
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 4 cut(s) 14, 444, 847, 895
HpyF10VI GCNNNNNNNGC 8 cut(s) 258, 450, 459, 496, 578, 645, 844, 951
HpyF3I CTNAG 2 cut(s) 449, 930
HpySE526I ACGT 1 cut(s) 366
Hsp92I GRCGYC 4 cut(s) 198, 504, 584, 749
Hsp92II CATG 3 cut(s) 373, 400, 890
HspAI GCGC 5 cut(s) 172, 198, 337, 345, 504
KasI GGCGCC 2 cut(s) 197, 503
KspI CCGCGG 2 cut(s) 163, 582
Kzo9I GATC 4 cut(s) 4, 55, 267, 551
LmnI GCTCC 5 cut(s) 146, 305, 577, 657, 782
Lsp1109I GCAGC 8 cut(s) 26, 374, 468, 648, 688, 695, 847, 907
LweI GCATC 2 cut(s) 841, 882
MaeI CTAG 2 cut(s) 249, 656
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 5 cut(s) 466, 475, 511, 745, 813
MalI GATC 4 cut(s) 6, 57, 269, 553
MbiI CCGCTC 1 cut(s) 652
MboI GATC 4 cut(s) 4, 55, 267, 551
MboII GAAGA 4 cut(s) 50, 137, 864, 896
MfeI CAATTG 1 cut(s) 920
MflI RGATCY 2 cut(s) 4, 55
MhlI GDGCHC 4 cut(s) 650, 771, 804, 947
MluCI AATT 5 cut(s) 118, 534, 786, 910, 920
MluI ACGCGT 1 cut(s) 351
Mly113I GGCGCC 2 cut(s) 198, 504
MlyI GAGTC 2 cut(s) 395, 585
MmeI TCCRAC 2 cut(s) 112, 610
MseI TTAA 1 cut(s) 149
MslI CAYNNNNRTG 1 cut(s) 743
MspA1I CMGCKG 6 cut(s) 162, 296, 453, 459, 581, 929
MspI CCGG 8 cut(s) 8, 144, 178, 195, 424, 501, 663, 783
MspR9I CCNGG 6 cut(s) 179, 501, 605, 722, 759, 783
MunI CAATTG 1 cut(s) 920
Mva1269I GAATGC 1 cut(s) 444
MvaI CCWGG 3 cut(s) 605, 722, 759
MvnI CGCG 6 cut(s) 162, 174, 337, 339, 353, 581
MwoI GCNNNNNNNGC 8 cut(s) 258, 450, 459, 496, 578, 645, 844, 951
NarI GGCGCC 2 cut(s) 198, 504
NciI CCSGG 3 cut(s) 179, 501, 783
NdeII GATC 4 cut(s) 4, 55, 267, 551
NlaIII CATG 3 cut(s) 373, 400, 890
NmuCI GTSAC 4 cut(s) 466, 511, 745, 813
NspV TTCGAA 1 cut(s) 531
OliI CACNNNNGTG 1 cut(s) 743
PaqCI CACCTGC 1 cut(s) 577
PcsI WCGNNNNNNNCGW 2 cut(s) 528, 636
PctI GAATGC 1 cut(s) 444
PfeI GAWTC 1 cut(s) 165
PleI GAGTC 2 cut(s) 395, 585
PluTI GGCGCC 2 cut(s) 201, 507
PpsI GAGTC 2 cut(s) 395, 585
Psp6I CCWGG 3 cut(s) 603, 720, 757
PspEI GGTNACC 1 cut(s) 511
PspGI CCWGG 3 cut(s) 603, 720, 757
PspOMI GGGCCC 2 cut(s) 646, 767
PspPI GGNCC 7 cut(s) 229, 243, 646, 647, 665, 767, 768
PsuI RGATCY 2 cut(s) 4, 55
RsaI GTAC 1 cut(s) 831
RsaNI GTAC 1 cut(s) 830
RseI CAYNNNNRTG 1 cut(s) 743
SacII CCGCGG 2 cut(s) 163, 582
SaqAI TTAA 1 cut(s) 149
Sau3AI GATC 4 cut(s) 4, 55, 267, 551
Sau96I GGNCC 7 cut(s) 229, 243, 646, 647, 665, 767, 768
SchI GAGTC 2 cut(s) 395, 585
ScrFI CCNGG 6 cut(s) 179, 501, 605, 722, 759, 783
SduI GDGCHC 4 cut(s) 650, 771, 804, 947
SfaNI GCATC 2 cut(s) 841, 882
SfoI GGCGCC 2 cut(s) 199, 505
Sfr303I CCGCGG 2 cut(s) 163, 582
SfuI TTCGAA 1 cut(s) 531
SgrBI CCGCGG 2 cut(s) 163, 582
SinI GGWCC 2 cut(s) 229, 243
SmiMI CAYNNNNRTG 1 cut(s) 743
Sse9I AATT 5 cut(s) 118, 534, 786, 910, 920
SspDI GGCGCC 2 cut(s) 197, 503
SspMI CTAG 2 cut(s) 249, 656
StyD4I CCNGG 6 cut(s) 177, 499, 603, 720, 757, 781
StyI CCWWGG 1 cut(s) 772
TaaI ACNGT 3 cut(s) 466, 524, 813
TaiI ACGT 1 cut(s) 369
TaqI TCGA 1 cut(s) 531
TasI AATT 5 cut(s) 118, 534, 786, 910, 920
TatI WGTACW 1 cut(s) 829
TauI GCSGC 4 cut(s) 264, 456, 462, 584
TfiI GAWTC 1 cut(s) 165
Tru1I TTAA 1 cut(s) 149
Tru9I TTAA 1 cut(s) 149
TseFI GTSAC 4 cut(s) 466, 511, 745, 813
TseI GCWGC 8 cut(s) 14, 387, 456, 636, 683, 701, 835, 895
Tsp45I GTSAC 4 cut(s) 466, 511, 745, 813
TspDTI ATGAA 2 cut(s) 50, 875
TspGWI ACGGA 1 cut(s) 30
VpaK11BI GGWCC 2 cut(s) 229, 243
XagI CCTNNNNNAGG 1 cut(s) 725
XapI RAATTY 3 cut(s) 118, 534, 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 214
XspI CTAG 2 cut(s) 249, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.