Rorug05G0495700

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
N/A
Physical Location & Seq
Forward (+)
67784395 .. 67786704
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0495700.1

Sequence Viewer

Length: 1104 bp
ATGGCAACCATATCATCATTCTTCAACAGGTTCCTCTCTTTCTTCCTCCTTCTCTTCCACCTCAGCTGCTTCACTATCACCCCCACCGCCGCCGCCGCCTCCACCACCAATCCCCACCGCCATAAACGCAGACTCACTTCATTCCTCTCCAAACCCTCTCTAAAACCCCAAAAGGCTCTTTCCTCCTCTTGGTCCTTTCTCAAGAAAGTCTTCAAGTCCTCCAAAACCCAAACCCACCACCTCTCCACGCCGCCTCGCTCCTCCAGCCACTCCCTCGTCTCCCTCGTCCTTCCCGCCCAAATCAAATCAGACCCTCCGCCCAAAAACTCCGATGACTCTGATATCTTCTCCGGCGACCACCACTTTTTCCCTCTCCGAAACGACATCTTCCCCTGCACAACTTGCGGCGAGATTTTCCATAAACCTCAGGCGCTCGACCACCACCAAGCAGTCAAGCACGCCGTGTCGGAGCTCCACAACGGCGACACCGGTAAGAACATAGTCCAGATCATATTCAAAACAGGCTGGACCGACACCCAGAAAAGCCCAGTGATCCACCGCATTCTAAAGATCCACAACAGCGTCAAGATACTTGCCAGGTTCGAAGAGTACAGAGAGTTAGTGAAGTCCAAAGCAGCGAGAAACGGCGCCGTTGGAAGAGACGAAAGGTGCGTAGCTGATGGGAACGAGCTGCTGAGATTTCACTGCTCGACTTTTCTCTGCGACTTGGGAGTCGGCGGGAGCTCCGGGATTTGCAACCAGCAGTATTGCAGCGTTTGCGGGATTATCAAGTCAGGGTTCTCACCCAAGTTGGACGGAATCTCCACGCTGTCGAGCAGCTCCAGGGCACACGTGGCCATCCCGGAGGAGATCGAGGAGGAGTTCAAGTTCATGAACGTGAAGCGGGCCATGCTGGTCTGCCGCGTCGTGGCGGGTCGGGTCGGGTGCGACTCTGATGATGACGTGGACAAGGAGGGTGGCGGGTTTGACTCGGTGGTGGGGAGGAGTGGGAGTGGGACCCAGATGAGGGTGGATGAGGAGGAGCTCTTGGTGTTTAACCCGAGGGCTGTGCTTCCCTGCTTTGTGATTGTGTATACCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

367

Amino Acids

40.55

Weight (kDa)

8.87

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 731
AccB1I GGYRCC 1 cut(s) 647
AccI GTMKAC 1 cut(s) 1094
AccII CGCG 1 cut(s) 924
AclWI GGATC 2 cut(s) 547, 565
AcoI YGGCCR 1 cut(s) 855
AcvI CACGTG 1 cut(s) 853
AcyI GRCGYC 1 cut(s) 648
AdeI CACNNNGTG 1 cut(s) 463
AfaI GTAC 1 cut(s) 611
AfiI CCNNNNNNNGG 4 cut(s) 189, 928, 1026, 1027
AflIII ACRYGT 1 cut(s) 850
AgeI ACCGGT 1 cut(s) 488
AgsI TTSAA 4 cut(s) 25, 214, 517, 886
AjiI CACGTC 1 cut(s) 964
AjnI CCWGG 2 cut(s) 596, 842
AloI GAACNNNNNNTCC 2 cut(s) 872, 904
AluBI AGCT 7 cut(s) 66, 472, 677, 691, 744, 840, 1045
AluI AGCT 7 cut(s) 66, 472, 677, 691, 744, 840, 1045
Alw21I GWGCWC 3 cut(s) 474, 746, 1047
Alw26I GTCTC 2 cut(s) 283, 654
AlwI GGATC 2 cut(s) 547, 565
Ama87I CYCGRG 1 cut(s) 1060
AoxI GGCC 2 cut(s) 855, 906
ApeKI GCWGC 5 cut(s) 66, 635, 691, 771, 837
AsiGI ACCGGT 1 cut(s) 488
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 2 cut(s) 433, 650
AspS9I GGNCC 4 cut(s) 192, 528, 906, 1017
AsuC2I CCSGG 2 cut(s) 748, 863
AsuHPI GGTGA 2 cut(s) 70, 795
AsuII TTCGAA 1 cut(s) 603
AvaI CYCGRG 1 cut(s) 1060
AvaII GGWCC 3 cut(s) 192, 528, 1017
AxyI CCTNAGG 1 cut(s) 426
BaeGI GKGCMC 1 cut(s) 850
BalI TGGCCA 1 cut(s) 857
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 3 cut(s) 474, 746, 1047
BbrPI CACGTG 1 cut(s) 853
BbsI GAAGAC 1 cut(s) 202
Bbv12I GWGCWC 3 cut(s) 474, 746, 1047
BbvCI CCTCAGC 1 cut(s) 62
BbvI GCAGC 5 cut(s) 53, 647, 678, 783, 849
BccI CCATC 2 cut(s) 674, 866
BceAI ACGGC 4 cut(s) 446, 496, 635, 661
BcgI CGANNNNNNTGC 2 cut(s) 1051, 1085
BciT130I CCWGG 2 cut(s) 598, 844
BcnI CCSGG 2 cut(s) 748, 863
BcoDI GTCTC 2 cut(s) 283, 654
BfoI RGCGCY 2 cut(s) 434, 651
Bme1390I CCNGG 4 cut(s) 598, 748, 844, 863
Bme18I GGWCC 3 cut(s) 192, 528, 1017
BmeT110I CYCGRG 1 cut(s) 1060
BmgBI CACGTC 1 cut(s) 964
BmgT120I GGNCC 4 cut(s) 192, 528, 906, 1017
BmiI GGNNCC 4 cut(s) 32, 649, 1018, 1019
BmrFI CCNGG 4 cut(s) 598, 748, 844, 863
BmrI ACTGGG 1 cut(s) 542
BmuI ACTGGG 1 cut(s) 542
BpiI GAAGAC 1 cut(s) 202
BpmI CTGGAG 2 cut(s) 247, 826
Bpu10I CCTNAGC 1 cut(s) 62
Bpu14I TTCGAA 1 cut(s) 603
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 2 cut(s) 748, 863
BsaAI YACGTR 1 cut(s) 853
BsaHI GRCGYC 1 cut(s) 648
BsaJI CCNNGG 2 cut(s) 843, 1061
BsaWI WCCGGW 1 cut(s) 488
BsaXI ACNNNNNCTCC 8 cut(s) 227, 257, 806, 836, 872, 902, 997, 1027
Bsc4I CCNNNNNNNGG 4 cut(s) 189, 928, 1026, 1027
Bse118I RCCGGY 1 cut(s) 488
Bse1I ACTGG 1 cut(s) 548
Bse21I CCTNAGG 1 cut(s) 426
BseBI CCWGG 2 cut(s) 598, 844
BseDI CCNNGG 2 cut(s) 843, 1061
BseGI GGATG 2 cut(s) 858, 1039
BseLI CCNNNNNNNGG 4 cut(s) 189, 928, 1026, 1027
BseMII CTCAG 3 cut(s) 76, 440, 686
BseNI ACTGG 1 cut(s) 548
BseRI GAGGAG 8 cut(s) 175, 250, 881, 890, 893, 1018, 1052, 1055
BseSI GKGCMC 1 cut(s) 850
BseXI GCAGC 5 cut(s) 53, 647, 678, 783, 849
BsgI GTGCAG 1 cut(s) 379
Bsh1236I CGCG 1 cut(s) 924
BshFI GGCC 2 cut(s) 857, 908
BshNI GGYRCC 1 cut(s) 647
BshTI ACCGGT 1 cut(s) 488
BsiHKAI GWGCWC 3 cut(s) 474, 746, 1047
BsiHKCI CYCGRG 1 cut(s) 1060
BsiSI CCGG 4 cut(s) 351, 489, 747, 863
BslFI GGGAC 1 cut(s) 1030
BslI CCNNNNNNNGG 4 cut(s) 189, 928, 1026, 1027
BsmAI GTCTC 2 cut(s) 283, 654
BsmBI CGTCTC 2 cut(s) 283, 654
BsmFI GGGAC 1 cut(s) 1030
BsmI GAATGC 1 cut(s) 561
BsnI GGCC 2 cut(s) 857, 908
BsoBI CYCGRG 1 cut(s) 1060
Bsp119I TTCGAA 1 cut(s) 603
Bsp1286I GDGCHC 4 cut(s) 474, 746, 850, 1047
Bsp143I GATC 4 cut(s) 507, 552, 570, 870
BspANI GGCC 2 cut(s) 857, 908
BspCNI CTCAG 3 cut(s) 75, 439, 687
BspFNI CGCG 1 cut(s) 924
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 4 cut(s) 32, 649, 1018, 1019
BspPI GGATC 2 cut(s) 547, 565
BspT104I TTCGAA 1 cut(s) 603
BspT107I GGYRCC 1 cut(s) 647
BsrFI RCCGGY 1 cut(s) 488
BsrI ACTGG 1 cut(s) 548
BssAI RCCGGY 1 cut(s) 488
BssECI CCNNGG 2 cut(s) 843, 1061
BssMI GATC 4 cut(s) 507, 552, 570, 870
BssNAI GTATAC 1 cut(s) 1095
BssNI GRCGYC 1 cut(s) 648
Bst1107I GTATAC 1 cut(s) 1095
Bst2UI CCWGG 2 cut(s) 598, 844
Bst4CI ACNGT 1 cut(s) 1099
Bst6I CTCTTC 3 cut(s) 59, 600, 652
BstACI GRCGYC 1 cut(s) 648
BstAPI GCANNNNNTGC 2 cut(s) 402, 777
BstBAI YACGTR 1 cut(s) 853
BstBI TTCGAA 1 cut(s) 603
BstC8I GCNNGC 2 cut(s) 459, 906
BstDEI CTNAG 3 cut(s) 62, 426, 695
BstF5I GGATG 2 cut(s) 858, 1039
BstFNI CGCG 1 cut(s) 924
BstH2I RGCGCY 2 cut(s) 434, 651
BstHHI GCGC 2 cut(s) 433, 650
BstKTI GATC 4 cut(s) 510, 555, 573, 873
BstMAI GTCTC 2 cut(s) 283, 654
BstMBI GATC 4 cut(s) 507, 552, 570, 870
BstMWI GCNNNNNNNGC 7 cut(s) 95, 126, 264, 402, 777, 854, 910
BstNI CCWGG 2 cut(s) 598, 844
BstSCI CCNGG 4 cut(s) 596, 746, 842, 861
BstSLI GKGCMC 1 cut(s) 850
BstUI CGCG 1 cut(s) 924
BstV1I GCAGC 5 cut(s) 53, 647, 678, 783, 849
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 1 cut(s) 570
BstYI RGATCY 1 cut(s) 570
BstZ17I GTATAC 1 cut(s) 1095
Bsu36I CCTNAGG 1 cut(s) 426
BsuRI GGCC 2 cut(s) 857, 908
BtrI CACGTC 1 cut(s) 964
BtsCI GGATG 2 cut(s) 858, 1039
BtsI GCAGTG 1 cut(s) 703
BtsIMutI CAGTG 2 cut(s) 555, 703
Cac8I GCNNGC 2 cut(s) 459, 906
CciI TCATGA 1 cut(s) 891
CfoI GCGC 2 cut(s) 433, 650
Cfr10I RCCGGY 1 cut(s) 488
Cfr13I GGNCC 4 cut(s) 192, 528, 906, 1017
CseI GACGC 2 cut(s) 571, 913
Csp6I GTAC 1 cut(s) 610
CspAI ACCGGT 1 cut(s) 488
CspCI CAANNNNNGTGG 2 cut(s) 958, 993
CviAII CATG 2 cut(s) 892, 910
CviQI GTAC 1 cut(s) 610
DdeI CTNAG 3 cut(s) 62, 426, 695
DinI GGCGCC 1 cut(s) 649
DpnI GATC 4 cut(s) 509, 554, 572, 872
DpnII GATC 4 cut(s) 507, 552, 570, 870
DraIII CACNNNGTG 1 cut(s) 463
DrdI GACNNNNNNGTC 1 cut(s) 731
DseDI GACNNNNNNGTC 1 cut(s) 731
EaeI YGGCCR 1 cut(s) 855
Eam1104I CTCTTC 3 cut(s) 59, 600, 652
EarI CTCTTC 3 cut(s) 59, 600, 652
EciI GGCGGA 1 cut(s) 306
Ecl136II GAGCTC 3 cut(s) 472, 744, 1045
Eco24I GRGCYC 3 cut(s) 474, 746, 1047
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 3 cut(s) 192, 528, 1017
Eco53kI GAGCTC 3 cut(s) 472, 744, 1045
Eco72I CACGTG 1 cut(s) 853
Eco81I CCTNAGG 1 cut(s) 426
Eco88I CYCGRG 1 cut(s) 1060
EcoICRI GAGCTC 3 cut(s) 472, 744, 1045
EcoO109I RGGNCCY 1 cut(s) 1017
EcoRII CCWGG 2 cut(s) 596, 842
EcoRV GATATC 1 cut(s) 343
EcoT38I GRGCYC 3 cut(s) 474, 746, 1047
EgeI GGCGCC 1 cut(s) 649
EheI GGCGCC 1 cut(s) 649
Esp3I CGTCTC 2 cut(s) 283, 654
FaeI CATG 2 cut(s) 895, 913
FaiI YATR 8 cut(s) 11, 123, 420, 500, 512, 893, 911, 1095
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FaqI GGGAC 1 cut(s) 1030
FatI CATG 2 cut(s) 891, 909
FauI CCCGC 6 cut(s) 301, 731, 773, 897, 925, 974
FblI GTMKAC 1 cut(s) 1094
FokI GGATG 2 cut(s) 845, 1046
FriOI GRGCYC 3 cut(s) 474, 746, 1047
GlaI GCGC 2 cut(s) 432, 649
GsuI CTGGAG 2 cut(s) 247, 826
HaeII RGCGCY 2 cut(s) 434, 651
HaeIII GGCC 2 cut(s) 857, 908
HapII CCGG 4 cut(s) 351, 489, 747, 863
HgaI GACGC 2 cut(s) 571, 913
HhaI GCGC 2 cut(s) 433, 650
Hin1I GRCGYC 1 cut(s) 648
Hin1II CATG 2 cut(s) 895, 913
Hin6I GCGC 2 cut(s) 431, 648
HinP1I GCGC 2 cut(s) 431, 648
HinfI GANTC 6 cut(s) 132, 335, 732, 819, 950, 989
HpaII CCGG 4 cut(s) 351, 489, 747, 863
HphI GGTGA 2 cut(s) 70, 795
Hpy166II GTNNAC 2 cut(s) 967, 1095
Hpy188I TCNGA 6 cut(s) 310, 331, 340, 377, 469, 955
Hpy188III TCNNGA 4 cut(s) 202, 505, 586, 892
Hpy8I GTNNAC 2 cut(s) 967, 1095
Hpy99I CGWCG 1 cut(s) 929
HpyAV CCTTC 2 cut(s) 59, 299
HpyCH4III ACNGT 1 cut(s) 1099
HpyCH4IV ACGT 3 cut(s) 852, 897, 963
HpyCH4V TGCA 3 cut(s) 396, 756, 771
HpyF10VI GCNNNNNNNGC 7 cut(s) 95, 126, 264, 402, 777, 854, 910
HpyF3I CTNAG 3 cut(s) 62, 426, 695
HpySE526I ACGT 3 cut(s) 852, 897, 963
Hsp92I GRCGYC 1 cut(s) 648
Hsp92II CATG 2 cut(s) 895, 913
HspAI GCGC 2 cut(s) 431, 648
KasI GGCGCC 1 cut(s) 647
KflI GGGWCCC 1 cut(s) 1017
Kzo9I GATC 4 cut(s) 507, 552, 570, 870
LmnI GCTCC 7 cut(s) 263, 469, 477, 741, 749, 845, 1042
Lsp1109I GCAGC 5 cut(s) 53, 647, 678, 783, 849
MaeII ACGT 3 cut(s) 852, 897, 963
MalI GATC 4 cut(s) 509, 554, 572, 872
MboI GATC 4 cut(s) 507, 552, 570, 870
MboII GAAGA 8 cut(s) 13, 34, 46, 202, 337, 379, 617, 669
MflI RGATCY 1 cut(s) 570
MhlI GDGCHC 4 cut(s) 474, 746, 850, 1047
MlsI TGGCCA 1 cut(s) 857
MluNI TGGCCA 1 cut(s) 857
Mly113I GGCGCC 1 cut(s) 648
MlyI GAGTC 5 cut(s) 126, 329, 741, 944, 983
MmeI TCCRAC 3 cut(s) 447, 634, 792
Mox20I TGGCCA 1 cut(s) 857
MroXI GAANNNNTTC 1 cut(s) 209
MscI TGGCCA 1 cut(s) 857
MseI TTAA 1 cut(s) 1056
MslI CAYNNNNRTG 1 cut(s) 896
Msp20I TGGCCA 1 cut(s) 857
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 4 cut(s) 351, 489, 747, 863
MspR9I CCNGG 4 cut(s) 598, 748, 844, 863
Mva1269I GAATGC 1 cut(s) 561
MvaI CCWGG 2 cut(s) 598, 844
MvnI CGCG 1 cut(s) 924
MwoI GCNNNNNNNGC 7 cut(s) 95, 126, 264, 402, 777, 854, 910
NarI GGCGCC 1 cut(s) 648
NciI CCSGG 2 cut(s) 748, 863
NdeII GATC 4 cut(s) 507, 552, 570, 870
NlaIII CATG 2 cut(s) 895, 913
NlaIV GGNNCC 4 cut(s) 32, 649, 1018, 1019
NspV TTCGAA 1 cut(s) 603
PagI TCATGA 1 cut(s) 891
PcsI WCGNNNNNNNCGW 2 cut(s) 282, 669
PctI GAATGC 1 cut(s) 561
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 1 cut(s) 819
PfoI TCCNGGA 2 cut(s) 746, 861
PinAI ACCGGT 1 cut(s) 488
PleI GAGTC 5 cut(s) 126, 329, 740, 944, 983
PluTI GGCGCC 1 cut(s) 651
PmaCI CACGTG 1 cut(s) 853
PmlI CACGTG 1 cut(s) 853
PpsI GAGTC 5 cut(s) 126, 329, 740, 944, 983
Ppu21I YACGTR 1 cut(s) 853
PpuMI RGGWCCY 1 cut(s) 1017
Psp124BI GAGCTC 3 cut(s) 474, 746, 1047
Psp5II RGGWCCY 1 cut(s) 1017
Psp6I CCWGG 2 cut(s) 596, 842
PspCI CACGTG 1 cut(s) 853
PspGI CCWGG 2 cut(s) 596, 842
PspN4I GGNNCC 4 cut(s) 32, 649, 1018, 1019
PspPI GGNCC 4 cut(s) 192, 528, 906, 1017
PspPPI RGGWCCY 1 cut(s) 1017
PsuI RGATCY 1 cut(s) 570
PvuII CAGCTG 1 cut(s) 66
RsaI GTAC 1 cut(s) 611
RsaNI GTAC 1 cut(s) 610
RseI CAYNNNNRTG 1 cut(s) 896
SacI GAGCTC 3 cut(s) 474, 746, 1047
SaqAI TTAA 1 cut(s) 1056
Sau3AI GATC 4 cut(s) 507, 552, 570, 870
Sau96I GGNCC 4 cut(s) 192, 528, 906, 1017
SchI GAGTC 5 cut(s) 126, 329, 741, 944, 983
ScrFI CCNGG 4 cut(s) 598, 748, 844, 863
SduI GDGCHC 4 cut(s) 474, 746, 850, 1047
SfoI GGCGCC 1 cut(s) 649
SfuI TTCGAA 1 cut(s) 603
SinI GGWCC 3 cut(s) 192, 528, 1017
SmiMI CAYNNNNRTG 1 cut(s) 896
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
SspDI GGCGCC 1 cut(s) 647
SstI GAGCTC 3 cut(s) 474, 746, 1047
StyD4I CCNGG 4 cut(s) 596, 746, 842, 861
TaaI ACNGT 1 cut(s) 1099
TaiI ACGT 3 cut(s) 855, 900, 966
TaqI TCGA 5 cut(s) 435, 603, 710, 833, 873
TaqII GACCGA 1 cut(s) 545
TatI WGTACW 1 cut(s) 609
TauI GCSGC 6 cut(s) 92, 95, 98, 253, 408, 924
TfiI GAWTC 1 cut(s) 819
Tru1I TTAA 1 cut(s) 1056
Tru9I TTAA 1 cut(s) 1056
TscAI CASTG 2 cut(s) 555, 710
TseI GCWGC 5 cut(s) 66, 635, 691, 771, 837
TspDTI ATGAA 3 cut(s) 129, 880, 908
TspGWI ACGGA 1 cut(s) 831
TspRI CASTG 2 cut(s) 555, 710
VpaK11BI GGWCC 3 cut(s) 192, 528, 1017
XcmI CCANNNNNNNNNTGG 1 cut(s) 850
XmiI GTMKAC 1 cut(s) 1094
XmnI GAANNNNTTC 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.