Rorug06G0115700
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
N/A
Physical Location & Seq
Forward (+)
15739072 .. 15740714
1643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0115700.1

Sequence Viewer

Length: 585 bp
ATGATTGAACAAATTGTGGGATTAGCAAAGGCAATGATAAAGCACAGTTTGAAGATTGAGGGCTTGGATGATGCAGTTGACATTGTTGGAACTGGTGGTGACGAAGCAAACACAGTAAACATTTCAACCGGGGCTTCAATACTTGCTGCAGCTTGTGGTGCAAAAGTTGCAAAGCAAGGTAATCGTTCAAGTTCCTCTGCATGTGGAAGTGCAGATGTATTGGAAGCTTTGGGAATAATAATTGACTTGGACCCTGAGGGGGTCACGAGATGTGTTAATGAAGTAGGCATTGGTTTTATGATGGCACCAAAGTATCATCCTGCAATGAATGTCGTTCGTCCCGTAAGGAAAAAGCTAAAAGTAAAGACTGTATTCAACATTTTGGGACCTATGTTGAATCCAGCTAGAGTTCCTTTTGCTGTTGTTGGTGTATACAAGGAAGAATTAGTCCTAAAGATGGCTAAAGCATTACAAAGCTTTGGCATGAAGAGAGCATTGGTTGTTCACTCGGAGGGCTTGGATGAAATGAGTCCCCTTGGTAAACTCTATCTTTTACATGAGATTATGATTATTTGGCAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

194

Amino Acids

20.73

Weight (kDa)

8.4

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycos_transf_3 PF00591 23 - 181 2e-71 Glycosyl transferase family, a/b domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 304
AccI GTMKAC 1 cut(s) 432
AfiI CCNNNNNNNGG 2 cut(s) 259, 457
AgsI TTSAA 7 cut(s) 8, 52, 126, 138, 189, 376, 397
AjuI GAANNNNNNNTTGG 4 cut(s) 273, 305, 479, 511
AluBI AGCT 5 cut(s) 152, 227, 355, 404, 477
AluI AGCT 5 cut(s) 152, 227, 355, 404, 477
ApeKI GCWGC 2 cut(s) 146, 149
AspS9I GGNCC 2 cut(s) 250, 386
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 110
AvaII GGWCC 2 cut(s) 250, 386
AxyI CCTNAGG 1 cut(s) 255
BaeI ACNNNNGTAYC 2 cut(s) 296, 329
BanI GGYRCC 1 cut(s) 304
BauI CACGAG 1 cut(s) 265
BbvI GCAGC 2 cut(s) 133, 161
BccI CCATC 2 cut(s) 295, 451
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BcnI CCSGG 1 cut(s) 130
BfaI CTAG 1 cut(s) 405
BfmI CTRYAG 1 cut(s) 147
BisI GCNGC 2 cut(s) 147, 150
BlsI GCNGC 2 cut(s) 148, 151
Bme1390I CCNGG 1 cut(s) 130
Bme18I GGWCC 2 cut(s) 250, 386
BmgT120I GGNCC 2 cut(s) 250, 386
BmiI GGNNCC 3 cut(s) 252, 306, 387
BmrFI CCNGG 1 cut(s) 130
BmsI GCATC 1 cut(s) 61
BpuMI CCSGG 1 cut(s) 130
BsaJI CCNNGG 2 cut(s) 129, 535
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 457
Bse1I ACTGG 1 cut(s) 97
Bse21I CCTNAGG 1 cut(s) 255
Bse3DI GCAATG 2 cut(s) 39, 330
BseDI CCNNGG 2 cut(s) 129, 535
BseGI GGATG 3 cut(s) 73, 316, 526
BseLI CCNNNNNNNGG 2 cut(s) 259, 457
BseMI GCAATG 2 cut(s) 39, 330
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 97
BseXI GCAGC 2 cut(s) 133, 161
BsgI GTGCAG 1 cut(s) 231
BshNI GGYRCC 1 cut(s) 304
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 3 cut(s) 324, 399, 516
BslI CCNNNNNNNGG 2 cut(s) 259, 457
BsmFI GGGAC 3 cut(s) 324, 399, 516
BspCNI CTCAG 1 cut(s) 247
BspLI GGNNCC 3 cut(s) 252, 306, 387
BspMAI CTGCAG 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 304
BsrDI GCAATG 2 cut(s) 39, 330
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 2 cut(s) 129, 535
BssNAI GTATAC 1 cut(s) 433
BssSI CACGAG 1 cut(s) 265
BssT1I CCWWGG 1 cut(s) 535
Bst1107I GTATAC 1 cut(s) 433
Bst2BI CACGAG 1 cut(s) 265
Bst4CI ACNGT 3 cut(s) 47, 115, 370
Bst6I CTCTTC 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 167
BstDEI CTNAG 1 cut(s) 255
BstF5I GGATG 3 cut(s) 73, 316, 526
BstMWI GCNNNNNNNGC 2 cut(s) 158, 167
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 1 cut(s) 128
BstSFI CTRYAG 1 cut(s) 147
BstV1I GCAGC 2 cut(s) 133, 161
BstZ17I GTATAC 1 cut(s) 433
Bsu36I CCTNAGG 1 cut(s) 255
BtsCI GGATG 3 cut(s) 73, 316, 526
Cfr13I GGNCC 2 cut(s) 250, 386
CviAII CATG 3 cut(s) 201, 484, 557
CviJI RGCY 9 cut(s) 63, 134, 152, 227, 355, 404, 461, 477, 516
CviKI_1 RGCY 9 cut(s) 63, 134, 152, 227, 355, 404, 461, 477, 516
DdeI CTNAG 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 482
EarI CTCTTC 1 cut(s) 482
Eco130I CCWWGG 1 cut(s) 535
Eco47I GGWCC 2 cut(s) 250, 386
Eco81I CCTNAGG 1 cut(s) 255
EcoO109I RGGNCCY 1 cut(s) 386
EcoT14I CCWWGG 1 cut(s) 535
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 3 cut(s) 204, 487, 560
FaiI YATR 7 cut(s) 202, 299, 392, 433, 485, 558, 566
FaqI GGGAC 3 cut(s) 324, 399, 516
FatI CATG 3 cut(s) 200, 483, 556
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 2 cut(s) 147, 150
FokI GGATG 3 cut(s) 80, 303, 533
Fsp4HI GCNGC 2 cut(s) 147, 150
FspBI CTAG 1 cut(s) 405
GluI GCNGC 2 cut(s) 147, 150
HapII CCGG 1 cut(s) 129
Hin1II CATG 3 cut(s) 204, 487, 560
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 2 cut(s) 225, 475
HinfI GANTC 2 cut(s) 397, 529
HpaII CCGG 1 cut(s) 129
HphI GGTGA 1 cut(s) 110
Hpy166II GTNNAC 5 cut(s) 79, 118, 433, 505, 542
Hpy188I TCNGA 1 cut(s) 511
Hpy188III TCNNGA 1 cut(s) 265
Hpy8I GTNNAC 5 cut(s) 79, 118, 433, 505, 542
HpyCH4III ACNGT 3 cut(s) 47, 115, 370
HpyCH4V TGCA 7 cut(s) 74, 149, 161, 170, 200, 212, 323
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 167
HpyF3I CTNAG 1 cut(s) 255
Hsp92II CATG 3 cut(s) 204, 487, 560
LpnPI CCDG 5 cut(s) 78, 142, 267, 333, 414
Lsp1109I GCAGC 2 cut(s) 133, 161
LweI GCATC 1 cut(s) 61
MaeI CTAG 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 98, 262
MboII GAAGA 3 cut(s) 64, 452, 499
MluCI AATT 4 cut(s) 12, 240, 443, 578
MlyI GAGTC 1 cut(s) 538
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 4 cut(s) 52, 205, 250, 505
MseI TTAA 1 cut(s) 276
MspI CCGG 1 cut(s) 129
MspR9I CCNGG 1 cut(s) 130
MwoI GCNNNNNNNGC 2 cut(s) 158, 167
NciI CCSGG 1 cut(s) 130
NlaIII CATG 3 cut(s) 204, 487, 560
NlaIV GGNNCC 3 cut(s) 252, 306, 387
NmuCI GTSAC 2 cut(s) 98, 262
NspI RCATGY 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 2 cut(s) 148, 151
PleI GAGTC 1 cut(s) 537
PpsI GAGTC 1 cut(s) 537
PpuMI RGGWCCY 1 cut(s) 386
Psp5II RGGWCCY 1 cut(s) 386
PspN4I GGNNCC 3 cut(s) 252, 306, 387
PspPI GGNCC 2 cut(s) 250, 386
PspPPI RGGWCCY 1 cut(s) 386
PstI CTGCAG 1 cut(s) 151
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 2 cut(s) 147, 150
Sau96I GGNCC 2 cut(s) 250, 386
SchI GAGTC 1 cut(s) 538
ScrFI CCNGG 1 cut(s) 130
SetI ASST 7 cut(s) 154, 181, 229, 357, 391, 406, 479
SfaNI GCATC 1 cut(s) 61
SfcI CTRYAG 1 cut(s) 147
SinI GGWCC 2 cut(s) 250, 386
Sse9I AATT 4 cut(s) 12, 240, 443, 578
SspMI CTAG 1 cut(s) 405
StyD4I CCNGG 1 cut(s) 128
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 3 cut(s) 47, 115, 370
TasI AATT 4 cut(s) 12, 240, 443, 578
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TseFI GTSAC 2 cut(s) 98, 262
TseI GCWGC 2 cut(s) 146, 149
Tsp45I GTSAC 2 cut(s) 98, 262
TspDTI ATGAA 4 cut(s) 294, 341, 500, 537
VpaK11BI GGWCC 2 cut(s) 250, 386
XceI RCATGY 1 cut(s) 204
XmiI GTMKAC 1 cut(s) 432
XspI CTAG 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.