Rorug06G0185500

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
N/A
Physical Location & Seq
Forward (+)
29389430 .. 29391029
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0185500.1

Sequence Viewer

Length: 651 bp
ATGGCGGCTCCTCCGATCAAATCGGTCCAGTGCTTCGGGACCTACTGCAAGGAAGGCCGCGGCTTGATCAAGATCAACGGCTGCCCAATCGAGCTCGTGGAGCCGGAGATCCTCCGCTTCAAGGCCTACGAGCCCATCCTTCTGCTCGGACAACACCGTTTCGCCGGCGTCGACATGAGGATCCGCGTCAAGGGAGGAGGTCACACTTCTCATATATACGCCATCCGTCAGAGCATCGCTAAGGCCTTGGTCGCTTATTACCAGAAGTTTGTGGATGAGCAGAGCAGGAAGGAAATCAAGGACATTCTCGTCAGGTATGACCGTACTCTTCTCGTCGCCTATCTAAGGCGCTGCGAGCCCAAGAAGTTTGGTGGTCGTGGTGCCAGAGCTAGTTTACATTTGTTATCTATTTCGAATGCAATTGTTGGAAAGGGGTTGATGTTAAACAAGGAAGGGTATGCTTCTATAGGAGGTGGTTCACCCTTGCGTAAGATGACAGATGAGCAGGCAAATGCACTTAAAATGGCTTTGGAATCAAAGGACATGCCGGTGAATGTCTATGTGGGAATGCGGTACTGGTTCCCGTTCACTGAGGAAGTTTTTCATCAAATTAAGAGGGACAGAATAACCAGATTAAGAGGGACAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

216

Amino Acids

24.46

Weight (kDa)

9.8

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S9 PF00380 15 - 131 2.5e-25 Ribosomal protein S9/S16
Ferrochelatase PF00762 149 - 212 1.1e-15 Ferrochelatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
AccB1I GGYRCC 1 cut(s) 380
AccI GTMKAC 1 cut(s) 171
AccII CGCG 2 cut(s) 60, 186
AciI CCGC 6 cut(s) 5, 58, 60, 115, 184, 571
AclWI GGATC 3 cut(s) 103, 175, 188
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 2 cut(s) 325, 575
AfiI CCNNNNNNNGG 3 cut(s) 121, 190, 345
AgsI TTSAA 1 cut(s) 121
AluBI AGCT 2 cut(s) 94, 389
AluI AGCT 2 cut(s) 94, 389
Alw21I GWGCWC 1 cut(s) 96
AlwI GGATC 3 cut(s) 103, 175, 188
AoxI GGCC 3 cut(s) 55, 123, 243
ApeKI GCWGC 2 cut(s) 81, 351
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 2 cut(s) 25, 39
AsuHPI GGTGA 2 cut(s) 471, 562
AsuII TTCGAA 1 cut(s) 413
AvaII GGWCC 2 cut(s) 25, 39
BamHI GGATCC 1 cut(s) 180
BanI GGYRCC 1 cut(s) 380
BanII GRGCYC 3 cut(s) 96, 135, 360
BauI CACGAG 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 68, 338
BccI CCATC 2 cut(s) 143, 230
BceAI ACGGC 1 cut(s) 94
BclI TGATCA 1 cut(s) 66
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 352
BisI GCNGC 5 cut(s) 6, 58, 61, 82, 352
BlsI GCNGC 5 cut(s) 7, 59, 62, 83, 353
Bme18I GGWCC 2 cut(s) 25, 39
BmgT120I GGNCC 2 cut(s) 25, 39
BmiI GGNNCC 6 cut(s) 9, 40, 102, 182, 382, 581
BmsI GCATC 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 413
BsaBI GATNNNNATC 1 cut(s) 71
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 58, 246
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 190, 345
Bse118I RCCGGY 2 cut(s) 164, 547
Bse1I ACTGG 2 cut(s) 28, 581
Bse8I GATNNNNATC 1 cut(s) 71
BseDI CCNNGG 2 cut(s) 58, 246
BseGI GGATG 3 cut(s) 135, 222, 280
BseJI GATNNNNATC 1 cut(s) 71
BseLI CCNNNNNNNGG 3 cut(s) 121, 190, 345
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 2 cut(s) 28, 581
BseRI GAGGAG 1 cut(s) 210
BseXI GCAGC 2 cut(s) 68, 338
Bsh1236I CGCG 2 cut(s) 60, 186
BshFI GGCC 3 cut(s) 57, 125, 245
BshNI GGYRCC 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 3 cut(s) 104, 165, 548
BslFI GGGAC 2 cut(s) 52, 632
BslI CCNNNNNNNGG 3 cut(s) 121, 190, 345
BsmFI GGGAC 2 cut(s) 52, 632
BsmI GAATGC 2 cut(s) 421, 573
BsnI GGCC 3 cut(s) 57, 125, 245
Bsp119I TTCGAA 1 cut(s) 413
Bsp1286I GDGCHC 3 cut(s) 96, 135, 360
Bsp143I GATC 5 cut(s) 15, 66, 72, 108, 180
BspACI CCGC 6 cut(s) 5, 58, 60, 115, 184, 571
BspANI GGCC 3 cut(s) 57, 125, 245
BspCNI CTCAG 1 cut(s) 583
BspFNI CGCG 2 cut(s) 60, 186
BspLI GGNNCC 6 cut(s) 9, 40, 102, 182, 382, 581
BspPI GGATC 3 cut(s) 103, 175, 188
BspT104I TTCGAA 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 380
BsrFI RCCGGY 2 cut(s) 164, 547
BsrI ACTGG 2 cut(s) 28, 581
BssAI RCCGGY 2 cut(s) 164, 547
BssECI CCNNGG 2 cut(s) 58, 246
BssMI GATC 5 cut(s) 15, 66, 72, 108, 180
BssNI GRCGYC 1 cut(s) 168
BssSI CACGAG 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 246
Bst2BI CACGAG 1 cut(s) 95
Bst4CI ACNGT 2 cut(s) 158, 323
Bst6I CTCTTC 1 cut(s) 333
BstACI GRCGYC 1 cut(s) 168
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 3 cut(s) 166, 356, 507
BstDEI CTNAG 3 cut(s) 240, 344, 591
BstDSI CCRYGG 1 cut(s) 58
BstENI CCTNNNNNAGG 1 cut(s) 343
BstF5I GGATG 3 cut(s) 135, 222, 280
BstFNI CGCG 2 cut(s) 60, 186
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 5 cut(s) 18, 69, 75, 111, 183
BstMBI GATC 5 cut(s) 15, 66, 72, 108, 180
BstMWI GCNNNNNNNGC 4 cut(s) 54, 100, 251, 355
BstNSI RCATGY 1 cut(s) 547
BstSFI CTRYAG 1 cut(s) 465
BstUI CGCG 2 cut(s) 60, 186
BstV1I GCAGC 2 cut(s) 68, 338
BstX2I RGATCY 2 cut(s) 108, 180
BstYI RGATCY 2 cut(s) 108, 180
BsuRI GGCC 3 cut(s) 57, 125, 245
BtgI CCRYGG 1 cut(s) 58
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 3 cut(s) 135, 222, 280
BtsIMutI CAGTG 2 cut(s) 35, 588
Cac8I GCNNGC 3 cut(s) 166, 356, 507
CfoI GCGC 1 cut(s) 351
Cfr10I RCCGGY 2 cut(s) 164, 547
Cfr13I GGNCC 2 cut(s) 25, 39
Cfr42I CCGCGG 1 cut(s) 61
CseI GACGC 2 cut(s) 157, 175
Csp6I GTAC 2 cut(s) 324, 574
CviAII CATG 2 cut(s) 175, 544
CviQI GTAC 2 cut(s) 324, 574
DdeI CTNAG 3 cut(s) 240, 344, 591
DpnI GATC 5 cut(s) 17, 68, 74, 110, 182
DpnII GATC 5 cut(s) 15, 66, 72, 108, 180
DrdI GACNNNNNNGTC 1 cut(s) 308
DseDI GACNNNNNNGTC 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 246
Eco147I AGGCCT 2 cut(s) 125, 245
Eco24I GRGCYC 3 cut(s) 96, 135, 360
Eco47I GGWCC 2 cut(s) 25, 39
Eco53kI GAGCTC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 94
EcoNI CCTNNNNNAGG 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 246
EcoT38I GRGCYC 3 cut(s) 96, 135, 360
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 2 cut(s) 178, 547
FaiI YATR 9 cut(s) 176, 213, 215, 217, 318, 459, 467, 545, 561
FaqI GGGAC 2 cut(s) 52, 632
FatI CATG 2 cut(s) 174, 543
FbaI TGATCA 1 cut(s) 66
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 5 cut(s) 6, 58, 61, 82, 352
FokI GGATG 3 cut(s) 122, 209, 287
FriOI GRGCYC 3 cut(s) 96, 135, 360
Fsp4HI GCNGC 5 cut(s) 6, 58, 61, 82, 352
FspBI CTAG 1 cut(s) 390
GlaI GCGC 1 cut(s) 350
GluI GCNGC 5 cut(s) 6, 58, 61, 82, 352
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 3 cut(s) 57, 125, 245
HapII CCGG 3 cut(s) 104, 165, 548
HgaI GACGC 2 cut(s) 157, 175
HhaI GCGC 1 cut(s) 351
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 2 cut(s) 178, 547
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 1 cut(s) 533
HpaII CCGG 3 cut(s) 104, 165, 548
HphI GGTGA 2 cut(s) 471, 562
Hpy166II GTNNAC 4 cut(s) 172, 395, 479, 588
Hpy188I TCNGA 3 cut(s) 15, 149, 231
Hpy188III TCNNGA 2 cut(s) 37, 70
Hpy8I GTNNAC 4 cut(s) 172, 395, 479, 588
Hpy99I CGWCG 2 cut(s) 173, 338
HpyAV CCTTC 4 cut(s) 47, 149, 283, 446
HpyCH4III ACNGT 2 cut(s) 158, 323
HpyCH4V TGCA 3 cut(s) 48, 419, 515
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 100, 251, 355
HpyF3I CTNAG 3 cut(s) 240, 344, 591
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 2 cut(s) 178, 547
HspAI GCGC 1 cut(s) 349
KroI GCCGGC 1 cut(s) 164
KroNI GCCGGC 1 cut(s) 166
Ksp22I TGATCA 1 cut(s) 66
KspI CCGCGG 1 cut(s) 61
Kzo9I GATC 5 cut(s) 15, 66, 72, 108, 180
LmnI GCTCC 2 cut(s) 13, 100
Lsp1109I GCAGC 2 cut(s) 68, 338
LweI GCATC 1 cut(s) 243
MaeI CTAG 1 cut(s) 390
MaeIII GTNAC 1 cut(s) 200
MalI GATC 5 cut(s) 17, 68, 74, 110, 182
MboI GATC 5 cut(s) 15, 66, 72, 108, 180
MboII GAAGA 1 cut(s) 320
MfeI CAATTG 1 cut(s) 420
MflI RGATCY 2 cut(s) 108, 180
MhlI GDGCHC 3 cut(s) 96, 135, 360
MluCI AATT 2 cut(s) 420, 609
MmeI TCCRAC 1 cut(s) 406
MnlI CCTC 9 cut(s) 21, 122, 171, 188, 191, 464, 586, 609, 632
MreI CGCCGGCG 1 cut(s) 164
MroNI GCCGGC 1 cut(s) 164
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 4 cut(s) 443, 519, 612, 635
MslI CAYNNNNRTG 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 3 cut(s) 104, 165, 548
MunI CAATTG 1 cut(s) 420
Mva1269I GAATGC 2 cut(s) 421, 573
MvnI CGCG 2 cut(s) 60, 186
MwoI GCNNNNNNNGC 4 cut(s) 54, 100, 251, 355
NaeI GCCGGC 1 cut(s) 166
NdeII GATC 5 cut(s) 15, 66, 72, 108, 180
NgoMIV GCCGGC 1 cut(s) 164
NlaIII CATG 2 cut(s) 178, 547
NlaIV GGNNCC 6 cut(s) 9, 40, 102, 182, 382, 581
NmuCI GTSAC 1 cut(s) 200
NspI RCATGY 1 cut(s) 547
NspV TTCGAA 1 cut(s) 413
PceI AGGCCT 2 cut(s) 125, 245
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 2 cut(s) 421, 573
PdiI GCCGGC 1 cut(s) 166
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 1 cut(s) 533
PkrI GCNGC 5 cut(s) 7, 59, 62, 83, 353
PpuMI RGGWCCY 1 cut(s) 39
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 39
PspN4I GGNNCC 6 cut(s) 9, 40, 102, 182, 382, 581
PspPI GGNCC 2 cut(s) 25, 39
PspPPI RGGWCCY 1 cut(s) 39
PsuI RGATCY 2 cut(s) 108, 180
RsaI GTAC 2 cut(s) 325, 575
RsaNI GTAC 2 cut(s) 324, 574
RseI CAYNNNNRTG 1 cut(s) 548
SacI GAGCTC 1 cut(s) 96
SacII CCGCGG 1 cut(s) 61
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 4 cut(s) 443, 519, 612, 635
SatI GCNGC 5 cut(s) 6, 58, 61, 82, 352
Sau3AI GATC 5 cut(s) 15, 66, 72, 108, 180
Sau96I GGNCC 2 cut(s) 25, 39
SduI GDGCHC 3 cut(s) 96, 135, 360
SetI ASST 6 cut(s) 44, 96, 202, 317, 391, 475
SfaNI GCATC 1 cut(s) 243
SfcI CTRYAG 1 cut(s) 465
Sfr303I CCGCGG 1 cut(s) 61
SfuI TTCGAA 1 cut(s) 413
SgrAI CRCCGGYG 1 cut(s) 164
SgrBI CCGCGG 1 cut(s) 61
SinI GGWCC 2 cut(s) 25, 39
SmiMI CAYNNNNRTG 1 cut(s) 548
Sse9I AATT 2 cut(s) 420, 609
SseBI AGGCCT 2 cut(s) 125, 245
SsiI CCGC 6 cut(s) 5, 58, 60, 115, 184, 571
SspMI CTAG 1 cut(s) 390
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 2 cut(s) 125, 245
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 2 cut(s) 158, 323
TaqI TCGA 3 cut(s) 90, 171, 413
TaqII GACCGA 1 cut(s) 13
TasI AATT 2 cut(s) 420, 609
TauI GCSGC 3 cut(s) 8, 60, 63
TfiI GAWTC 1 cut(s) 533
Tru1I TTAA 4 cut(s) 443, 519, 612, 635
Tru9I TTAA 4 cut(s) 443, 519, 612, 635
TscAI CASTG 2 cut(s) 35, 595
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 2 cut(s) 81, 351
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 1 cut(s) 593
TspGWI ACGGA 1 cut(s) 215
TspRI CASTG 2 cut(s) 35, 595
VpaK11BI GGWCC 2 cut(s) 25, 39
XagI CCTNNNNNAGG 1 cut(s) 343
XceI RCATGY 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.