Rorug07G0144200

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
N/A
Physical Location & Seq
Reverse (-)
11375997 .. 11381814
5818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0144200.1

Sequence Viewer

Length: 2121 bp
ATGGGCTCTTACAAGGACGCTGACCCAGCCCTAGGGTACCTAACCCGAAAGGATACGGAGGTCAAGCTACCACGGCCGACTAGGGTTAAGAATAAGACACCAGCCCCTGTTCAGATTACCGCCGAGCAAATCCTCCGGGAGGCTCGTGAGCGGCAGGAGGCGGAAATCCGGCCTCCCAAGCAGAAAATCACTGACCCATCTGAGCTTGCGGATTATCGTCTGCGAAAACGAAAGGAATTTGAGGATTTAATTCGGAGAGTGCGGTGGAATGTGAGTGTTTGGATCAAGTATGCGCAGTGGGAGGAGTCCCAGAAGGACTTCAAGCGTGCACGGTCTGTTTGGGAGCGTGCCCTTGAGGTGGATTATCGGAATCATACGCTATGGCTCAAGTATGCGGAGGTGGAGATGAAGAACAAGTTCATTAATCATGCTAGGAATGTGTGGGACCGTGCTGTTCAACTCTTGCCTAGAGTGGACCAGCTCTGGTACAAGTACATTCACATGGAGGAGATGATTGGGAATGTGGCTGGTGCTCGGCAAATTTTCGAGAGGTGGATGACTTGGATGCCGGACCAGCAAGGCTGGCTATCCTTCATCAAATTTGAGCTTCGGTATAATGAAGTTGAGCGTGCTAGAGCGATTTTCGAGCGGTTTGTACAATGTCATCCGAAAGTTGCAGCTTGGATTCGGTTTGCCAAATTTGAGATGAAGAATGGTGATGTTGCGAGGGCGAGGAATGTGTATGAGAGGGCAGTGGAGAAGTTAGCTGATGATGAGGAGGCCGAGCAGCTGTTTGTGGCTTTCGCTGAATTTGAAGAGCGGTGCAAAGAGACCGAGCGAGCTCGGTGTATCTATAAATTTGCACTGGATCACATACCCAAAGGAAGAGCTGAGGATTTGTATAAGAAGTTTGTATCTTTTGAGAAGCAATATGGCGATAGAGAAGGGATTGAGGATGCCATTGTGGGAAAGAGGAGGTTTCAGTATGAGGATGAGGTTAGGAAGAATCCTCTTAATTATGACTCTTGGTTTGATTATATAAGGCTGGAAGAGAGTGCAGGGAATAAGGACAGAATCAGAGAGGTTTATGAGCGAGCTGTTGCTAATGTTCCTCCTGCTCCTGAGAAGCGGTATTGGCAGCGATATATTTATCTGTGGATTAATTATGCACTTTACGAGGAACTTGATACTGGAGATGTGGAACGTACACGAGATGTCTATAGGGAGTGCCTTAAATTGATTCCTCATGAGAAATTTTCATTTGCAAAAATATGGCTTCTTGCTGCTCAGTTTGAGATACGGCAGCTAAATCTCACGGGTGCACGCCAGATTCTTGGTACTGCAATAGGCAAGGCACCAAAAGATAAGATTTTCAAGAAGTATATTGAGATTGAGCTGCAACTTGCAAATATAGATCGTTGTCGGAAGTTATATGAAAAGTATCTAGAGTGGGCACCACACAATTGCTATGCATGGAGCAAGTATGCAGAGTTAGAGAAATCTTTGGGTGAGACAGAACGAGCAAGGGCCCTTTTTGAACTCGCCATCAGCCAATTAGAACTTGACATGCCTGAGTTGTTATGGAAGGCATACATCGACTTTGAACTGTCAGAATTTGAGTTTGAAAGAACCAGACAACTTTATGAGAGACTCCTAGACCGGACAAAACATCTAAAGGTGTGGATCAGTTATGCAAAATTTGAGGCATCTGCTATGGTCGGGGGAAATGACGAGGATTCAGATTTGGCAGAAGCTCCTTCCGAGGATGATATAATTGAAGAGAAGAAACAATGTATTGAGCGTGCTAGAAGGGTTTTTGAGAAAGCTCTTAACTATTTTAGAACTTCAGCACCTGAATTGAAGGAAGAAAGAGGTATGTTGCTAGAAGAATGGTTTAATATGGAGGCTAGCTTTGGGGATCTGGGTGATATTAGCCTAGTTCAATCTAAGCTGCCAAAGAAACTGAAGAAGAGGAGGGCAATAGTAACCGAAGACGGTCCTGCAGGGTACGAGGAATACATTGATTACCTGTTCCCAGAAGAAAGTCAGACGACGAATCTCAAGATTTTGGAAGCTGCCTACAAGTGGAAGAAGCAAAAAGTTTCTTCTGATGAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

706

Amino Acids

84.49

Weight (kDa)

6.42

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAT_PRP39_C PF23241 76 - 191 7.2e-14 PRP39 C-terminal HAT repeat
HAT_PRP39_N PF23240 77 - 187 8e-09 PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 79 - 224 5.3e-18 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
Suf PF05843 80 - 319 7.6e-12 Suppressor of forked protein (Suf)
HAT_Syf1_CNRKL1_C PF23231 92 - 153 8.1e-08 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_Syf1_CNRKL1_C PF23231 125 - 240 1.1e-10 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_N PF23240 173 - 304 3.6e-12 PRP39 N-terminal HAT repeat
HAT_PRP39_C PF23241 193 - 294 5.3e-13 PRP39 C-terminal HAT repeat
HAT PF02184 207 - 235 8.7e-12 HAT (Half-A-TPR) repeat
HAT_Syf1_CNRKL1_C PF23231 241 - 321 8.4e-11 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_N PF23240 324 - 447 3.1e-07 PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 327 - 486 1.3e-14 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_C PF23241 339 - 449 2.3e-08 PRP39 C-terminal HAT repeat
HAT_Syf1_CNRKL1_C PF23231 399 - 537 1e-12 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 458 - 571 2.8e-07 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N PF23240 458 - 555 3.2e-11 PRP39 N-terminal HAT repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 294
Acc65I GGTACC 1 cut(s) 36
AccB1I GGYRCC 3 cut(s) 36, 1354, 1453
AccBSI CCGCTC 3 cut(s) 151, 649, 820
AciI CCGC 9 cut(s) 120, 151, 161, 209, 262, 395, 649, 820, 1129
AclWI GGATC 4 cut(s) 290, 876, 1691, 1926
AcoI YGGCCR 1 cut(s) 74
AcsI RAATTY 9 cut(s) 236, 540, 599, 698, 809, 857, 1253, 1613, 1697
AcuI CTGAAG 2 cut(s) 1830, 1985
AfaI GTAC 7 cut(s) 38, 488, 494, 657, 1207, 1339, 2009
AfiI CCNNNNNNNGG 4 cut(s) 32, 139, 358, 2085
Alw21I GWGCWC 4 cut(s) 331, 535, 844, 1324
Alw26I GTCTC 3 cut(s) 824, 1505, 1642
Alw44I GTGCAC 2 cut(s) 327, 1320
AlwI GGATC 4 cut(s) 290, 876, 1691, 1926
AlwNI CAGNNNCTG 2 cut(s) 107, 1853
AoxI GGCC 4 cut(s) 74, 170, 780, 1527
ApaI GGGCCC 1 cut(s) 1531
ApaLI GTGCAC 2 cut(s) 327, 1320
ApeKI GCWGC 8 cut(s) 677, 787, 1138, 1283, 1303, 1396, 1951, 2075
ApoI RAATTY 9 cut(s) 236, 540, 599, 698, 809, 857, 1253, 1613, 1697
ArsI GACNNNNNNTTYG 2 cut(s) 1013, 1045
AseI ATTAAT 2 cut(s) 423, 1161
Asp700I GAANNNNTTC 2 cut(s) 317, 416
Asp718I GGTACC 1 cut(s) 36
AspA2I CCTAGG 1 cut(s) 31
AspLEI GCGC 1 cut(s) 295
AspS9I GGNCC 6 cut(s) 445, 475, 571, 1527, 1528, 1997
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 3 cut(s) 728, 1520, 1937
AsuNHI GCTAGC 1 cut(s) 1907
AvaII GGWCC 4 cut(s) 445, 475, 571, 1997
AvrII CCTAGG 1 cut(s) 31
BaeGI GKGCMC 5 cut(s) 331, 352, 1324, 1456, 1531
BaeI ACNNNNGTAYC 2 cut(s) 45, 78
BanI GGYRCC 3 cut(s) 36, 1354, 1453
BanII GRGCYC 3 cut(s) 8, 844, 1531
BauI CACGAG 2 cut(s) 144, 1209
BbsI GAAGAC 1 cut(s) 1998
Bbv12I GWGCWC 4 cut(s) 331, 535, 844, 1324
BbvCI CCTCAGC 1 cut(s) 891
BbvI GCAGC 8 cut(s) 689, 799, 1150, 1270, 1315, 1383, 1938, 2062
BccI CCATC 2 cut(s) 205, 1553
BceAI ACGGC 2 cut(s) 89, 1316
BciVI GTATCC 1 cut(s) 46
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 3 cut(s) 824, 1505, 1642
BfmI CTRYAG 2 cut(s) 1219, 2001
BfuI GTATCC 1 cut(s) 46
BisI GCNGC 9 cut(s) 152, 678, 788, 1139, 1284, 1304, 1397, 1952, 2076
BlnI CCTAGG 1 cut(s) 31
BlsI GCNGC 9 cut(s) 153, 679, 789, 1140, 1285, 1305, 1398, 1953, 2077
Bme1390I CCNGG 1 cut(s) 137
Bme18I GGWCC 4 cut(s) 445, 475, 571, 1997
BmgT120I GGNCC 6 cut(s) 445, 475, 571, 1527, 1528, 1997
BmiI GGNNCC 5 cut(s) 38, 446, 1356, 1455, 1529
BmrFI CCNGG 1 cut(s) 137
BmsI GCATC 3 cut(s) 555, 946, 1715
BmtI GCTAGC 1 cut(s) 1911
BpiI GAAGAC 1 cut(s) 1998
BpmI CTGGAG 1 cut(s) 1212
Bpu10I CCTNAGC 1 cut(s) 891
BpuEI CTTGAG 3 cut(s) 371, 374, 2045
BpuMI CCSGG 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 824
BsaJI CCNNGG 3 cut(s) 31, 71, 1761
BsaWI WCCGGW 1 cut(s) 1659
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 139, 358, 2085
Bse1I ACTGG 2 cut(s) 870, 1195
BseDI CCNNGG 3 cut(s) 31, 71, 1761
BseGI GGATG 6 cut(s) 561, 570, 664, 961, 997, 1771
BseLI CCNNNNNNNGG 4 cut(s) 32, 139, 358, 2085
BseMII CTCAG 5 cut(s) 192, 882, 1113, 1301, 1563
BseNI ACTGG 2 cut(s) 870, 1195
BseRI GAGGAG 5 cut(s) 317, 521, 791, 988, 1987
BseSI GKGCMC 5 cut(s) 331, 352, 1324, 1456, 1531
BseX3I CGGCCG 1 cut(s) 74
BseXI GCAGC 8 cut(s) 689, 799, 1150, 1270, 1315, 1383, 1938, 2062
BseYI CCCAGC 1 cut(s) 25
BsgI GTGCAG 1 cut(s) 1077
Bsh1285I CGRYCG 1 cut(s) 77
BshFI GGCC 4 cut(s) 76, 172, 782, 1529
BshNI GGYRCC 3 cut(s) 36, 1354, 1453
BsiEI CGRYCG 1 cut(s) 77
BsiHKAI GWGCWC 4 cut(s) 331, 535, 844, 1324
BsiSI CCGG 4 cut(s) 136, 169, 569, 1660
BslFI GGGAC 2 cut(s) 292, 458
BslI CCNNNNNNNGG 4 cut(s) 32, 139, 358, 2085
BsmAI GTCTC 3 cut(s) 824, 1505, 1642
BsmFI GGGAC 2 cut(s) 292, 458
BsnI GGCC 4 cut(s) 76, 172, 782, 1529
Bso31I GGTCTC 1 cut(s) 824
Bsp120I GGGCCC 1 cut(s) 1527
Bsp1286I GDGCHC 8 cut(s) 8, 331, 352, 535, 844, 1324, 1456, 1531
Bsp1407I TGTACA 1 cut(s) 655
Bsp143I GATC 5 cut(s) 282, 868, 1414, 1683, 1918
BspACI CCGC 9 cut(s) 120, 151, 161, 209, 262, 395, 649, 820, 1129
BspANI GGCC 4 cut(s) 76, 172, 782, 1529
BspCNI CTCAG 5 cut(s) 193, 883, 1114, 1300, 1564
BspHI TCATGA 1 cut(s) 1246
BspLI GGNNCC 5 cut(s) 38, 446, 1356, 1455, 1529
BspMAI CTGCAG 1 cut(s) 2005
BspOI GCTAGC 1 cut(s) 1911
BspPI GGATC 4 cut(s) 290, 876, 1691, 1926
BspQI GCTCTTC 2 cut(s) 810, 880
BspT107I GGYRCC 3 cut(s) 36, 1354, 1453
BspTNI GGTCTC 1 cut(s) 824
BsrBI CCGCTC 3 cut(s) 151, 649, 820
BsrGI TGTACA 1 cut(s) 655
BsrI ACTGG 2 cut(s) 870, 1195
BssECI CCNNGG 3 cut(s) 31, 71, 1761
BssMI GATC 5 cut(s) 282, 868, 1414, 1683, 1918
BssSI CACGAG 2 cut(s) 144, 1209
BssT1I CCWWGG 1 cut(s) 31
Bst2BI CACGAG 2 cut(s) 144, 1209
Bst4CI ACNGT 4 cut(s) 333, 449, 1608, 1997
Bst6I CTCTTC 5 cut(s) 810, 880, 1044, 1773, 1964
BstAUI TGTACA 1 cut(s) 655
BstDEI CTNAG 6 cut(s) 201, 891, 1122, 1287, 1572, 1947
BstDSI CCRYGG 1 cut(s) 71
BstENI CCTNNNNNAGG 1 cut(s) 137
BstF5I GGATG 6 cut(s) 561, 570, 664, 961, 997, 1771
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 5 cut(s) 285, 871, 1417, 1686, 1921
BstMAI GTCTC 3 cut(s) 824, 1505, 1642
BstMBI GATC 5 cut(s) 282, 868, 1414, 1683, 1918
BstMCI CGRYCG 1 cut(s) 77
BstMWI GCNNNNNNNGC 6 cut(s) 26, 73, 178, 574, 583, 1135
BstNSI RCATGY 1 cut(s) 1570
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 2 cut(s) 1219, 2001
BstSLI GKGCMC 5 cut(s) 331, 352, 1324, 1456, 1531
BstV1I GCAGC 8 cut(s) 689, 799, 1150, 1270, 1315, 1383, 1938, 2062
BstV2I GAAGAC 1 cut(s) 1998
BstX2I RGATCY 1 cut(s) 1918
BstXI CCANNNNNNTGG 1 cut(s) 1334
BstYI RGATCY 1 cut(s) 1918
BstZI CGGCCG 1 cut(s) 74
BsuI GTATCC 1 cut(s) 46
BsuRI GGCC 4 cut(s) 76, 172, 782, 1529
BtgI CCRYGG 1 cut(s) 71
BtsCI GGATG 6 cut(s) 561, 570, 664, 961, 997, 1771
BtsI GCAGTG 2 cut(s) 302, 759
BtsIMutI CAGTG 4 cut(s) 189, 302, 759, 863
CaiI CAGNNNCTG 2 cut(s) 107, 1853
CciI TCATGA 1 cut(s) 1246
CfoI GCGC 1 cut(s) 295
Cfr13I GGNCC 6 cut(s) 445, 475, 571, 1527, 1528, 1997
CseI GACGC 1 cut(s) 26
Csp6I GTAC 7 cut(s) 37, 487, 493, 656, 1206, 1338, 2008
CviAII CATG 5 cut(s) 428, 502, 1247, 1473, 1567
CviQI GTAC 7 cut(s) 37, 487, 493, 656, 1206, 1338, 2008
DdeI CTNAG 6 cut(s) 201, 891, 1122, 1287, 1572, 1947
DpnI GATC 5 cut(s) 284, 870, 1416, 1685, 1920
DpnII GATC 5 cut(s) 282, 868, 1414, 1683, 1918
EaeI YGGCCR 1 cut(s) 74
EagI CGGCCG 1 cut(s) 74
Eam1104I CTCTTC 5 cut(s) 810, 880, 1044, 1773, 1964
EarI CTCTTC 5 cut(s) 810, 880, 1044, 1773, 1964
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 842
EclXI CGGCCG 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 3 cut(s) 8, 844, 1531
Eco31I GGTCTC 1 cut(s) 824
Eco47I GGWCC 4 cut(s) 445, 475, 571, 1997
Eco52I CGGCCG 1 cut(s) 74
Eco53kI GAGCTC 1 cut(s) 842
Eco57I CTGAAG 2 cut(s) 1830, 1985
EcoICRI GAGCTC 1 cut(s) 842
EcoNI CCTNNNNNAGG 1 cut(s) 137
EcoO109I RGGNCCY 2 cut(s) 1527, 1528
EcoT14I CCWWGG 1 cut(s) 31
EcoT22I ATGCAT 1 cut(s) 1474
EcoT38I GRGCYC 3 cut(s) 8, 844, 1531
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 5 cut(s) 431, 505, 1250, 1476, 1570
FaqI GGGAC 2 cut(s) 292, 458
FatI CATG 5 cut(s) 427, 501, 1246, 1472, 1566
Fnu4HI GCNGC 9 cut(s) 152, 678, 788, 1139, 1284, 1304, 1397, 1952, 2076
FokI GGATG 6 cut(s) 568, 577, 651, 968, 1004, 1778
FriOI GRGCYC 3 cut(s) 8, 844, 1531
Fsp4HI GCNGC 9 cut(s) 152, 678, 788, 1139, 1284, 1304, 1397, 1952, 2076
FspI TGCGCA 1 cut(s) 294
GlaI GCGC 1 cut(s) 294
GluI GCNGC 9 cut(s) 152, 678, 788, 1139, 1284, 1304, 1397, 1952, 2076
GsaI CCCAGC 1 cut(s) 29
GsuI CTGGAG 1 cut(s) 1212
HaeIII GGCC 4 cut(s) 76, 172, 782, 1529
HapII CCGG 4 cut(s) 136, 169, 569, 1660
HgaI GACGC 1 cut(s) 26
HhaI GCGC 1 cut(s) 295
Hin1II CATG 5 cut(s) 431, 505, 1250, 1476, 1570
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HpaII CCGG 4 cut(s) 136, 169, 569, 1660
HphI GGTGA 3 cut(s) 728, 1520, 1937
Hpy166II GTNNAC 4 cut(s) 329, 475, 1208, 1322
Hpy188III TCNNGA 7 cut(s) 146, 547, 1121, 1247, 1375, 1445, 2062
Hpy8I GTNNAC 4 cut(s) 329, 475, 1208, 1322
Hpy99I CGWCG 1 cut(s) 2056
HpyAV CCTTC 7 cut(s) 307, 601, 938, 1579, 1767, 1803, 1855
HpyCH4III ACNGT 4 cut(s) 333, 449, 1608, 1997
HpyCH4IV ACGT 1 cut(s) 1204
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 73, 178, 574, 583, 1135
HpyF3I CTNAG 6 cut(s) 201, 891, 1122, 1287, 1572, 1947
HpySE526I ACGT 1 cut(s) 1204
Hsp92II CATG 5 cut(s) 431, 505, 1250, 1476, 1570
HspAI GCGC 1 cut(s) 293
KpnI GGTACC 1 cut(s) 40
Kzo9I GATC 5 cut(s) 282, 868, 1414, 1683, 1918
LguI GCTCTTC 2 cut(s) 810, 880
LmnI GCTCC 4 cut(s) 343, 1123, 1476, 1759
Lsp1109I GCAGC 8 cut(s) 689, 799, 1150, 1270, 1315, 1383, 1938, 2062
LweI GCATC 3 cut(s) 555, 946, 1715
MaeII ACGT 1 cut(s) 1204
MaeIII GTNAC 1 cut(s) 1984
MalI GATC 5 cut(s) 284, 870, 1416, 1685, 1920
MbiI CCGCTC 3 cut(s) 151, 649, 820
MboI GATC 5 cut(s) 282, 868, 1414, 1683, 1918
MfeI CAATTG 1 cut(s) 1462
MflI RGATCY 1 cut(s) 1918
MhlI GDGCHC 8 cut(s) 8, 331, 352, 535, 844, 1324, 1456, 1531
MlyI GAGTC 3 cut(s) 314, 1016, 1644
MmeI TCCRAC 1 cut(s) 1403
Mph1103I ATGCAT 1 cut(s) 1474
MroXI GAANNNNTTC 2 cut(s) 317, 416
MseI TTAA 8 cut(s) 87, 248, 423, 1014, 1161, 1233, 1830, 1896
MslI CAYNNNNRTG 1 cut(s) 500
MspA1I CMGCKG 1 cut(s) 790
MspI CCGG 4 cut(s) 136, 169, 569, 1660
MspR9I CCNGG 1 cut(s) 137
MunI CAATTG 1 cut(s) 1462
MwoI GCNNNNNNNGC 6 cut(s) 26, 73, 178, 574, 583, 1135
NciI CCSGG 1 cut(s) 137
NdeII GATC 5 cut(s) 282, 868, 1414, 1683, 1918
NheI GCTAGC 1 cut(s) 1907
NlaIII CATG 5 cut(s) 431, 505, 1250, 1476, 1570
NlaIV GGNNCC 5 cut(s) 38, 446, 1356, 1455, 1529
NmeAIII GCCGAG 3 cut(s) 148, 514, 808
NsbI TGCGCA 1 cut(s) 294
NsiI ATGCAT 1 cut(s) 1474
NspI RCATGY 1 cut(s) 1570
PagI TCATGA 1 cut(s) 1246
PciSI GCTCTTC 2 cut(s) 810, 880
PdmI GAANNNNTTC 2 cut(s) 317, 416
PfeI GAWTC 8 cut(s) 370, 685, 1006, 1074, 1240, 1330, 1736, 2056
PfoI TCCNGGA 1 cut(s) 135
PkrI GCNGC 9 cut(s) 153, 679, 789, 1140, 1285, 1305, 1398, 1953, 2077
PleI GAGTC 3 cut(s) 313, 1016, 1644
PpsI GAGTC 3 cut(s) 313, 1016, 1644
PshBI ATTAAT 2 cut(s) 423, 1161
Psp124BI GAGCTC 1 cut(s) 844
PspFI CCCAGC 1 cut(s) 25
PspN4I GGNNCC 5 cut(s) 38, 446, 1356, 1455, 1529
PspOMI GGGCCC 1 cut(s) 1527
PspPI GGNCC 6 cut(s) 445, 475, 571, 1527, 1528, 1997
PstI CTGCAG 1 cut(s) 2005
PstNI CAGNNNCTG 2 cut(s) 107, 1853
PsuI RGATCY 1 cut(s) 1918
PvuII CAGCTG 1 cut(s) 790
RsaI GTAC 7 cut(s) 38, 488, 494, 657, 1207, 1339, 2009
RsaNI GTAC 7 cut(s) 37, 487, 493, 656, 1206, 1338, 2008
RseI CAYNNNNRTG 1 cut(s) 500
SacI GAGCTC 1 cut(s) 844
SapI GCTCTTC 2 cut(s) 810, 880
SaqAI TTAA 8 cut(s) 87, 248, 423, 1014, 1161, 1233, 1830, 1896
SatI GCNGC 9 cut(s) 152, 678, 788, 1139, 1284, 1304, 1397, 1952, 2076
Sau3AI GATC 5 cut(s) 282, 868, 1414, 1683, 1918
Sau96I GGNCC 6 cut(s) 445, 475, 571, 1527, 1528, 1997
SbfI CCTGCAGG 1 cut(s) 2005
SchI GAGTC 3 cut(s) 314, 1016, 1644
ScrFI CCNGG 1 cut(s) 137
SdaI CCTGCAGG 1 cut(s) 2005
SduI GDGCHC 8 cut(s) 8, 331, 352, 535, 844, 1324, 1456, 1531
SfaNI GCATC 3 cut(s) 555, 946, 1715
SfcI CTRYAG 2 cut(s) 1219, 2001
SinI GGWCC 4 cut(s) 445, 475, 571, 1997
SmiMI CAYNNNNRTG 1 cut(s) 500
SmlI CTYRAG 3 cut(s) 353, 386, 2060
SmoI CTYRAG 3 cut(s) 353, 386, 2060
Sse8387I CCTGCAGG 1 cut(s) 2005
SsiI CCGC 9 cut(s) 120, 151, 161, 209, 262, 395, 649, 820, 1129
SstI GAGCTC 1 cut(s) 844
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 4 cut(s) 333, 449, 1608, 1997
TaiI ACGT 1 cut(s) 1207
TaqI TCGA 3 cut(s) 546, 645, 1596
TaqII GACCGA 1 cut(s) 848
TatI WGTACW 2 cut(s) 492, 655
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 8 cut(s) 370, 685, 1006, 1074, 1240, 1330, 1736, 2056
Tru1I TTAA 8 cut(s) 87, 248, 423, 1014, 1161, 1233, 1830, 1896
Tru9I TTAA 8 cut(s) 87, 248, 423, 1014, 1161, 1233, 1830, 1896
TscAI CASTG 4 cut(s) 196, 302, 759, 870
TseI GCWGC 8 cut(s) 677, 787, 1138, 1283, 1303, 1396, 1951, 2075
TspDTI ATGAA 7 cut(s) 409, 422, 583, 633, 722, 1248, 1449
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 4 cut(s) 196, 302, 759, 870
VneI GTGCAC 2 cut(s) 327, 1320
VpaK11BI GGWCC 4 cut(s) 445, 475, 571, 1997
VspI ATTAAT 2 cut(s) 423, 1161
XagI CCTNNNNNAGG 1 cut(s) 137
XapI RAATTY 9 cut(s) 236, 540, 599, 698, 809, 857, 1253, 1613, 1697
XbaI TCTAGA 1 cut(s) 1444
XceI RCATGY 1 cut(s) 1570
XmaJI CCTAGG 1 cut(s) 31
XmnI GAANNNNTTC 2 cut(s) 317, 416
Zsp2I ATGCAT 1 cut(s) 1474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.