Rorug07G0227400

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
N/A
Physical Location & Seq
Reverse (-)
20717978 .. 20740995
23018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0227400.1

Sequence Viewer

Length: 1533 bp
ATGACGAGACTACTATCGAATTCGAATTATGATTATAATGTTGCAACATATTCTAACTTTCGTAAAGTTATGAATTATTTAGAGCTCTTGAAGAGCTTATATGAGACATATCAATACACAAATATATTGATGTGTATGGCTAGCTGTGCAAAAGAAAATGAAGACATACTAACTTGTGCATGGAGAGTGCTGAACTATGTGTGGTTAAGGCCAAAAAAACTAGAAAGATGCTTAAGGAAGCAAGGCCTCAAAGGCAACTCCTACAGGCTTTTGATCGGAGACATGAAAGAGAGCTTTATGATGCTGAAACAAGCACAATCTAAACCAATCAACCTCTCATCCTGCCATGATATCGCACCACGAGTCATCCCTTTTGTGCATCAAACTGTGAAAAACTATGGTTTTGCAATCTATGAGGGTGAGAAATGGGCTAGACACCGAACTATTATCAACCCAGCATTTCATTTAGAGAAGCTAAAGCGTATGTTACCGGCCGTTTCTCAAAGTTGTGGTGAGATGATTAATGAATGGGAGACATTGCTTACCGAAAATGGTTCATTTGAGTTGGACGTGTGGCCTTATCTCCAAAATTTGACGGGTGATGTGATATCTCGAACAGCATTTGGAAGTAGTTACAAAGAAGGACTAAAAATATTCGAACTCTTGAGGGAACAAGCAGTACTTGTAACAAAAACTTCACATTTTGTTAATATTCCAGGGTGGAGGTTCCTACCAACTAAGATGAACAAGAGGATGAAGCAAAATGCCAAAGAAGTACAAGGTTTACTGGAGGGAATTATAAATAAAAGAAAAGAGGCAATTAAGGGTGGTGAAGCAACTAAAGATGACTTGTTAGGAATACTTTTGGAATCCAATTTTAAGAAAATTCAAGAACATGGGAACCGAAAAAACATTGGACTGAGTCTTCAAGACATCATTGATGAGTGTAAACTGTTTTACTTTGGAGGGCAAGAAACCACTTCAGTATTAGTTGTTTGGACAATAGTTTTGCTATCTCAAAATCCGAATTGGCAAACTCGTGCAAGAGAAGAGGTTCTGCAAGTATTTGGAAACAAAAGACCAGACTTTCAGGGGATGACACAACTAAAAGTTATGACCATGGTTTTACGTGAAGTTCTTCGGTTATATCCACCAGTAGCTTCTTTGAATCGAACCACAACACAACTTGGAACGTTATCATTACCAGCTGGAGTTGAAATGTCCATACCCATATTGCTTATTCATCACAATAAGAAACTGTGGGGTGATGATGCGGAAGAGTTTAATCCAGAAAGATTTTCAGAGGGAGTTTCAAAGGCAACTAAGGGACAAATATTTTCGTTCTTCCCTTTTGGGGGAGGTCCTCGCATTTGCATTGGTCAAAATTTTGCTATGATGGAAGCAAAAGTTGTGCTATCATTGATTTTGCAACACTTCACCTTTGAGCTTTCTCCATCATATGCTCATGCTCCTTCCTCATTTATAACTCTCCAGCCACAATACGGCGTTCATATCGTCTTAAAAAAACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

510

Amino Acids

58.5

Weight (kDa)

9.32

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 60 - 486 1.9e-80 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 36, 800, 1484
AciI CCGC 1 cut(s) 1274
AclI AACGTT 2 cut(s) 1193, 1528
AcoI YGGCCR 1 cut(s) 492
AcsI RAATTY 4 cut(s) 19, 589, 885, 1384
AcuI CTGAAG 1 cut(s) 966
AfaI GTAC 2 cut(s) 681, 777
AfiI CCNNNNNNNGG 3 cut(s) 1354, 1355, 1502
AflII CTTAAG 1 cut(s) 232
AflIII ACRYGT 1 cut(s) 570
AgsI TTSAA 6 cut(s) 91, 890, 929, 1168, 1217, 1314
AjiI CACGTC 1 cut(s) 571
AjnI CCWGG 1 cut(s) 715
AluBI AGCT 8 cut(s) 85, 96, 144, 294, 475, 1160, 1208, 1447
AluI AGCT 8 cut(s) 85, 96, 144, 294, 475, 1160, 1208, 1447
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 3 cut(s) 98, 273, 527
AoxI GGCC 4 cut(s) 209, 244, 492, 575
ApoI RAATTY 4 cut(s) 19, 589, 885, 1384
ArsI GACNNNNNNTTYG 2 cut(s) 991, 1023
AseI ATTAAT 1 cut(s) 522
Asp700I GAANNNNTTC 2 cut(s) 1053, 1137
AspS9I GGNCC 1 cut(s) 1361
AsuHPI GGTGA 6 cut(s) 431, 524, 611, 842, 1277, 1429
AsuII TTCGAA 2 cut(s) 23, 657
AsuNHI GCTAGC 1 cut(s) 140
AvaII GGWCC 1 cut(s) 1361
BanII GRGCYC 1 cut(s) 87
BauI CACGAG 2 cut(s) 360, 1038
BbsI GAAGAC 2 cut(s) 168, 917
Bbv12I GWGCWC 1 cut(s) 87
BccI CCATC 2 cut(s) 1390, 1462
BceAI ACGGC 2 cut(s) 479, 1519
BciT130I CCWGG 1 cut(s) 717
BcoDI GTCTC 3 cut(s) 98, 273, 527
BfaI CTAG 3 cut(s) 141, 221, 432
BfmI CTRYAG 1 cut(s) 262
BfrI CTTAAG 1 cut(s) 232
BglI GCCNNNNNGGC 1 cut(s) 252
BmcAI AGTACT 1 cut(s) 681
Bme1390I CCNGG 1 cut(s) 717
Bme18I GGWCC 1 cut(s) 1361
BmgBI CACGTC 1 cut(s) 571
BmgT120I GGNCC 1 cut(s) 1361
BmiI GGNNCC 2 cut(s) 728, 902
BmrFI CCNGG 1 cut(s) 717
BmsI GCATC 4 cut(s) 218, 291, 388, 1261
BmtI GCTAGC 1 cut(s) 144
BpiI GAAGAC 2 cut(s) 168, 917
BpmI CTGGAG 3 cut(s) 809, 1230, 1475
Bpu14I TTCGAA 2 cut(s) 23, 657
BpuEI CTTGAG 1 cut(s) 685
BsaAI YACGTR 1 cut(s) 1130
BsaJI CCNNGG 2 cut(s) 716, 1119
Bsc4I CCNNNNNNNGG 3 cut(s) 1354, 1355, 1502
Bse118I RCCGGY 1 cut(s) 490
Bse1I ACTGG 2 cut(s) 792, 1154
Bse3DI GCAATG 1 cut(s) 536
BseBI CCWGG 1 cut(s) 717
BseDI CCNNGG 2 cut(s) 716, 1119
BseGI GGATG 4 cut(s) 338, 366, 759, 1101
BseLI CCNNNNNNNGG 3 cut(s) 1354, 1355, 1502
BseMI GCAATG 1 cut(s) 536
BseMII CTCAG 1 cut(s) 911
BseNI ACTGG 2 cut(s) 792, 1154
BseX3I CGGCCG 1 cut(s) 492
BseYI CCCAGC 1 cut(s) 454
Bsh1285I CGRYCG 1 cut(s) 495
BshFI GGCC 4 cut(s) 211, 246, 494, 577
BsiEI CGRYCG 1 cut(s) 495
BsiHKAI GWGCWC 1 cut(s) 87
BsiSI CCGG 1 cut(s) 491
BslFI GGGAC 1 cut(s) 1341
BslI CCNNNNNNNGG 3 cut(s) 1354, 1355, 1502
BsmAI GTCTC 3 cut(s) 98, 273, 527
BsmFI GGGAC 1 cut(s) 1341
BsnI GGCC 4 cut(s) 211, 246, 494, 577
Bsp119I TTCGAA 2 cut(s) 23, 657
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 1 cut(s) 273
Bsp19I CCATGG 1 cut(s) 1119
BspACI CCGC 1 cut(s) 1274
BspANI GGCC 4 cut(s) 211, 246, 494, 577
BspCNI CTCAG 1 cut(s) 912
BspLI GGNNCC 2 cut(s) 728, 902
BspOI GCTAGC 1 cut(s) 144
BspQI GCTCTTC 1 cut(s) 86
BspT104I TTCGAA 2 cut(s) 23, 657
BspTI CTTAAG 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 536
BsrFI RCCGGY 1 cut(s) 490
BsrI ACTGG 2 cut(s) 792, 1154
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 2 cut(s) 716, 1119
BssMI GATC 1 cut(s) 273
BssSI CACGAG 2 cut(s) 360, 1038
BssT1I CCWWGG 1 cut(s) 1119
Bst2BI CACGAG 2 cut(s) 360, 1038
Bst2UI CCWGG 1 cut(s) 717
Bst4CI ACNGT 3 cut(s) 388, 954, 1260
Bst6I CTCTTC 3 cut(s) 86, 1044, 1272
BstAFI CTTAAG 1 cut(s) 232
BstBAI YACGTR 1 cut(s) 1130
BstBI TTCGAA 2 cut(s) 23, 657
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 3 cut(s) 738, 920, 1323
BstDSI CCRYGG 1 cut(s) 1119
BstF5I GGATG 4 cut(s) 338, 366, 759, 1101
BstKTI GATC 1 cut(s) 276
BstMAI GTCTC 3 cut(s) 98, 273, 527
BstMBI GATC 1 cut(s) 273
BstMCI CGRYCG 1 cut(s) 495
BstMWI GCNNNNNNNGC 2 cut(s) 146, 252
BstNI CCWGG 1 cut(s) 717
BstSCI CCNGG 1 cut(s) 715
BstSFI CTRYAG 1 cut(s) 262
BstV2I GAAGAC 2 cut(s) 168, 917
BstZI CGGCCG 1 cut(s) 492
BsuRI GGCC 4 cut(s) 211, 246, 494, 577
BtgI CCRYGG 1 cut(s) 1119
BtrI CACGTC 1 cut(s) 571
BtsCI GGATG 4 cut(s) 338, 366, 759, 1101
Cac8I GCNNGC 1 cut(s) 142
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 1 cut(s) 1361
Csp6I GTAC 2 cut(s) 680, 776
CviAII CATG 6 cut(s) 180, 283, 347, 896, 1120, 1466
CviQI GTAC 2 cut(s) 680, 776
DdeI CTNAG 3 cut(s) 738, 920, 1323
DpnI GATC 1 cut(s) 275
DpnII GATC 1 cut(s) 273
EaeI YGGCCR 1 cut(s) 492
EagI CGGCCG 1 cut(s) 492
Eam1104I CTCTTC 3 cut(s) 86, 1044, 1272
EarI CTCTTC 3 cut(s) 86, 1044, 1272
Ecl136II GAGCTC 1 cut(s) 85
EclXI CGGCCG 1 cut(s) 492
Eco130I CCWWGG 1 cut(s) 1119
Eco147I AGGCCT 1 cut(s) 246
Eco24I GRGCYC 1 cut(s) 87
Eco32I GATATC 2 cut(s) 352, 609
Eco47I GGWCC 1 cut(s) 1361
Eco52I CGGCCG 1 cut(s) 492
Eco53kI GAGCTC 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 966
EcoICRI GAGCTC 1 cut(s) 85
EcoO109I RGGNCCY 1 cut(s) 1361
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 715
EcoRV GATATC 2 cut(s) 352, 609
EcoT14I CCWWGG 1 cut(s) 1119
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 1119
FaeI CATG 6 cut(s) 183, 286, 350, 899, 1123, 1469
FalI AAGNNNNNCTT 2 cut(s) 666, 698
FaqI GGGAC 1 cut(s) 1341
FatI CATG 6 cut(s) 179, 282, 346, 895, 1119, 1465
FauNDI CATATG 1 cut(s) 1459
FokI GGATG 4 cut(s) 325, 353, 766, 1108
FriOI GRGCYC 1 cut(s) 87
FspBI CTAG 3 cut(s) 141, 221, 432
GsaI CCCAGC 1 cut(s) 458
GsuI CTGGAG 3 cut(s) 809, 1230, 1475
HaeIII GGCC 4 cut(s) 211, 246, 494, 577
HapII CCGG 1 cut(s) 491
Hin1II CATG 6 cut(s) 183, 286, 350, 899, 1123, 1469
HinfI GANTC 4 cut(s) 363, 869, 922, 1168
HpaII CCGG 1 cut(s) 491
HphI GGTGA 6 cut(s) 431, 524, 611, 842, 1277, 1429
Hpy166II GTNNAC 2 cut(s) 785, 950
Hpy188I TCNGA 3 cut(s) 278, 1026, 1303
Hpy188III TCNNGA 6 cut(s) 88, 612, 664, 890, 929, 1289
Hpy8I GTNNAC 2 cut(s) 785, 950
HpyAV CCTTC 2 cut(s) 635, 1482
HpyCH4III ACNGT 3 cut(s) 388, 954, 1260
HpyCH4IV ACGT 4 cut(s) 570, 1129, 1193, 1528
HpyCH4V TGCA 9 cut(s) 44, 149, 179, 379, 407, 1043, 1060, 1374, 1429
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 252
HpyF3I CTNAG 3 cut(s) 738, 920, 1323
HpySE526I ACGT 4 cut(s) 570, 1129, 1193, 1528
Hsp92II CATG 6 cut(s) 183, 286, 350, 899, 1123, 1469
Kzo9I GATC 1 cut(s) 273
LguI GCTCTTC 1 cut(s) 86
LmnI GCTCC 1 cut(s) 1474
LweI GCATC 4 cut(s) 218, 291, 388, 1261
MaeI CTAG 3 cut(s) 141, 221, 432
MaeII ACGT 4 cut(s) 570, 1129, 1193, 1528
MaeIII GTNAC 3 cut(s) 486, 632, 685
MalI GATC 1 cut(s) 275
MboI GATC 1 cut(s) 273
MboII GAAGA 7 cut(s) 103, 173, 917, 1061, 1130, 1289, 1336
MhlI GDGCHC 1 cut(s) 87
MlyI GAGTC 2 cut(s) 372, 931
MmeI TCCRAC 1 cut(s) 546
MroXI GAANNNNTTC 2 cut(s) 1053, 1137
MseI TTAA 9 cut(s) 206, 233, 522, 708, 822, 879, 1284, 1520, 1531
MspA1I CMGCKG 1 cut(s) 1208
MspCI CTTAAG 1 cut(s) 232
MspI CCGG 1 cut(s) 491
MspR9I CCNGG 1 cut(s) 717
MvaI CCWGG 1 cut(s) 717
MwoI GCNNNNNNNGC 2 cut(s) 146, 252
NcoI CCATGG 1 cut(s) 1119
NdeI CATATG 1 cut(s) 1459
NdeII GATC 1 cut(s) 273
NheI GCTAGC 1 cut(s) 140
NlaIII CATG 6 cut(s) 183, 286, 350, 899, 1123, 1469
NlaIV GGNNCC 2 cut(s) 728, 902
NspV TTCGAA 2 cut(s) 23, 657
PceI AGGCCT 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 86
PdmI GAANNNNTTC 2 cut(s) 1053, 1137
PfeI GAWTC 2 cut(s) 869, 1168
PflFI GACNNNGTC 1 cut(s) 921
PleI GAGTC 2 cut(s) 371, 930
PpsI GAGTC 2 cut(s) 371, 930
Ppu21I YACGTR 1 cut(s) 1130
PpuMI RGGWCCY 1 cut(s) 1361
PshBI ATTAAT 1 cut(s) 522
PsiI TTATAA 3 cut(s) 36, 800, 1484
Psp124BI GAGCTC 1 cut(s) 87
Psp1406I AACGTT 2 cut(s) 1193, 1528
Psp5II RGGWCCY 1 cut(s) 1361
Psp6I CCWGG 1 cut(s) 715
PspFI CCCAGC 1 cut(s) 454
PspGI CCWGG 1 cut(s) 715
PspN4I GGNNCC 2 cut(s) 728, 902
PspPI GGNCC 1 cut(s) 1361
PspPPI RGGWCCY 1 cut(s) 1361
PsrI GAACNNNNNNTAC 2 cut(s) 663, 695
PsyI GACNNNGTC 1 cut(s) 921
PvuII CAGCTG 1 cut(s) 1208
RsaI GTAC 2 cut(s) 681, 777
RsaNI GTAC 2 cut(s) 680, 776
SacI GAGCTC 1 cut(s) 87
SapI GCTCTTC 1 cut(s) 86
SaqAI TTAA 9 cut(s) 206, 233, 522, 708, 822, 879, 1284, 1520, 1531
Sau3AI GATC 1 cut(s) 273
Sau96I GGNCC 1 cut(s) 1361
ScaI AGTACT 1 cut(s) 681
SchI GAGTC 2 cut(s) 372, 931
ScrFI CCNGG 1 cut(s) 717
SduI GDGCHC 1 cut(s) 87
SfaNI GCATC 4 cut(s) 218, 291, 388, 1261
SfcI CTRYAG 1 cut(s) 262
SfuI TTCGAA 2 cut(s) 23, 657
SinI GGWCC 1 cut(s) 1361
SmlI CTYRAG 2 cut(s) 232, 664
SmoI CTYRAG 2 cut(s) 232, 664
SseBI AGGCCT 1 cut(s) 246
SsiI CCGC 1 cut(s) 1274
SspI AATATT 3 cut(s) 654, 712, 1335
SspMI CTAG 3 cut(s) 141, 221, 432
SstI GAGCTC 1 cut(s) 87
StuI AGGCCT 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 715
StyI CCWWGG 1 cut(s) 1119
TaaI ACNGT 3 cut(s) 388, 954, 1260
TaiI ACGT 4 cut(s) 573, 1132, 1196, 1531
TaqI TCGA 5 cut(s) 17, 23, 613, 657, 1171
TatI WGTACW 2 cut(s) 679, 775
TfiI GAWTC 2 cut(s) 869, 1168
Tru1I TTAA 9 cut(s) 206, 233, 522, 708, 822, 879, 1284, 1520, 1531
Tru9I TTAA 9 cut(s) 206, 233, 522, 708, 822, 879, 1284, 1520, 1531
Tth111I GACNNNGTC 1 cut(s) 921
Vha464I CTTAAG 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 1361
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 4 cut(s) 19, 589, 885, 1384
XmnI GAANNNNTTC 2 cut(s) 1053, 1137
XspI CTAG 3 cut(s) 141, 221, 432
ZrmI AGTACT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.