Rh1AG071300

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
N/A
Physical Location & Seq
Reverse (-)
11934769 .. 11948617
13849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG071300.1

Sequence Viewer

Length: 4092 bp
ATGAATCTAAAACCATCCAATTTACTTTTGGATGCGAATGGTCATGCTGTGGTTTCTGATTATGGAGTTGCTGCTATTCTTAAGAAACCTTCCTGTCGCAAAACTCGATCAGAATGTGACACCTCAAGGATCCATTCGTGTATGGAGTGTACAATGCTAAGTCCGCACTATGCAGCACCAGAGGCATGGGAGCCGGTGAAGAAGTCACTGAATCCATTCTGGGATGAAGCTATTGGTATCTCTGCAGAGTCAGATGCATGGAGTTTTGGTTGTACATTGGTAGAAATGTGCACCGGTTCCATCCCATGGGCTGGTTTGAGCACAGAGGAAATTTACAAGGCGGTGGTCAAGGCTCGAAAACTGCCTCCACAATATGCAAGTGTAGTAGGTGTTGGAATACCTAGGGAATTGTGGAAAATGATTGGTGAGTGCCTGCAGTACAAGGCATCAAAAAGACCATCCTTTAATTTAATGCTAGCAACTTTCCTCCGCCATTTGCAGGAGATACCGCGGAGTTCTCCTGCTAGTCCTGATAATGACGTAGCTAAAAGCTTTGGATCCAATGTGAAGGAACCATCGCCTGTATCCTATTCAAAAGTATTTCAGGGTAATCCTGCCCTTCTGCATAGACTTGTGTCCGAAGGGGATGTATATGGTGTTAGAGATCTGCTTGGAAAGGCTGCAGCGGGAAGTGACAACAGTGTAATATCGTCTCTATTAGAAGCTCAAAATGCTGATGGCCAAACTGCTCTTCATTTGGCCTGTAGACGTGGTAGTGCAGAACTTGTCGATGCTATTTTGGAGTATAGAGAGGCAAATGTAGATGTGTTGGACAAAGATGGGGATCCTCCACTTGTTTTTGCTCTAGTAGCTGGATCCCCAGAGTGTGTTCATGTTCTCATTAAAAGAGGTGCTAATGTGAGATCTCGGTTGCGGGAGGGCTTTGGTCCCTCTGTTGCTCATGTTTGTGCTTATCATGGCCAGCCTGATTGTATGCATGAGTTACTAATGGCTGGAGCTGATCCTAATGCGGTGGATGAGGAAGGGGAATCTGTACTCCATAGAGCTATTACCAAGAAGTATACAGATTGTGCACTTGTGGTTTTGGAAAATGGGGGCTGTAGGTCAATGACTGTTCTAAATTCAGAAAAAATGACGCCATTGCACTTGTGTGTGCAAACATGGAATGTTGCTGTTGTTAGAAGATGGGTGGAAGTTGCAACACCAGAAGAGATTACCGACGCAATTGATATACCAAGTCCAGTTGGCACTGCACTGTGTATGGCTGCTGCTCTAAAGAAAGATCATGAAATTGAAGGAAGAGAATTGGTGCGTATTTTGCTTGCTTCTGGAGCAGATCCAACTGCTCAGGATGCCCAGCATGGACGTACAGCTTTGCATACGGCTTCAATGGCCAATGACGTTGAGTTGGTGAAGATTATACTTGATGCGGGAGTTGATGTGAACATCCGAAATGCACAAAATACAATTCCACTTCATGTAGCACTGGCCAGAGGGGCGAAGTCATGTGTTGGGATGCTTCTATCCGCTGGAGCAAATTATAATATGCAGGATGATGAAGGGGACAATGCTTTCCATATAGCAGCAGATGCAGCAAAAATGATACGTGAAAATCTTGAATGGCTTATTGTCATGCTTAGAAATCCTGATGCTTCTGTTGAAGCCAGAAATCACAGTGGCAAGACATTGCGTGACTTTCTGGAAGCTCTTCCCCGGGAATGGGTATCTGAAGACCTGATGGAGGCACTTATTAATAGGGGAGTTTATCTGTCTCCTACAATATTTGAAGTGGGGGATTGGATCAAATTTAAAAGAAGTATCACAAATCCGACATATGGATGGCAAGGTGCAAAGCATAGAAGTGTAGGCTTCGTGCAAAGTGTCCCAGATAAGGACAATCTAATAGTATCATTTTGTTCTGGAGAGGCTCATGAGGCTCGTGTCTTAGCAAATGAAGTTATAAAAGTTATTCCATTAGACAGGGGGCAGCATGTCCAGCTTAAACCCGATGTTAAAGAGCCAAGGTTTGGGTGGCGTGGGCAATCACGTGATAGCATTGGTACTGTATTATGTGTTGATGATGATGGTATCCTACGTGTTGGGTTTCCTGGAGCCTCCAGAGGATGGAAAGCTGATCCTGCAGAAATGGAGAGAGTTGAAGAATTCAAAGTTGGGGACTGGGTTCGTATTCGCCCGACTCTCACAACAGCAAAGCATGGATTAGGATCTGTTACACCTGGGAGCATTGGTATTGTGTATTGTATACGACCAGATAGTAGTCTGTTGTTAGAATTAAGTTATCTTCCAAATCCATGGCATTGTGAACCAGAGGAGGTTGAGCCTGTAATCCCCTTCAGGATTGGTGACAGGGTATGTGTGAAGCGATCAGTTGCAGAACCTAGGTATGCATGGGGTGGTGAGACCCACCATAGTGTTGGAAGAATAAGTGAGATAGAGAATGATGGCCTCCTGGTTATTGAAATTCCAAACCGACCAATATCATGGCAGGCTGATCCTTCTGACATGGAGAAGTTGGAAGATTTCAAGGTAGGAGATTGGGTTAGAGTCAAAGCTTCAGTACCCTCCCCCAAATACGGATGGGAGGATATCACTAGGAGTAGTATTGGGATAATCCATAGCTTGGAGGAAGATGGTGATATGGGTGTTGCTTTTTGCTTCAGGAGCAAGCCTTTTTCTTGTTCTGTCACAGATGTGGAAAAGCTACCTCCTTTTGAGGTGGGACAAGAAATTCATATTCTGTCATCTGTCAGTCAGCCGCGACTTGGATGGTCAAATGAAAGTCCTGCGACTGTTGGAAAAATAGATAGAATTGACATGGATGGAGCTTTAAATGTAAGGGTGCCTGGCAGACAGAGCTTGTGGAAAGTTTCTCCAGGAGATGCTGAACGACTCTCTGGATTTGAAGTTGGTGACTGGGTACGATCAAAACCAAGCCTGGGAACTCGACCAAGTTATGACTGGAACAGTATTGGAAAAGAAAGTTTAGCTGTTGTTCACAGTGTACAAGACACAGGTTATCTTGAGTTGGCTTGCTGTTTCCGTAAAGGCAGGTGGATAACTCATTACACAGACGTTGAAAAGGTTCCATGCTTCAAAGTTGGCCAATATGTTCGGTTTCGTTCTGGTTTAGTTGAACCAAGATGGGGATGGAGGGGGGCTCAACCTGATTCTCGAGGTATTATAACTAGTATTCATGCTGATGGGGAAGTGAGAGTGGCTTTCCCTGGTTTGCCAGGGTTGTGGAGAGGAGATCCTGCTGATTTTGAGATAGAGCAGATTTATGAAGTTGGAGAGTGGGTGAGATTAGAAGATCATGCAAATATGTGGAAATCTGTTGGGCCAGGGAGTGTTGGCGTGGTGCAAGGATTAGGATATGAGGGAGATAAATGGGATGGAACCACTTTTGTAGGATTCTGCGGGGAGCAAGAGAGATGGATTGGGCCTACTTCTGATCTTGCAAGGGCAAACAGGCTCTTGGTTGGCCAGAAGGTTAGGGTTAAATTATCTGTCAAGCAGCCCCGATTTGGATGGTCAGGCCACAGCCATGCAAGTGTTGGAACTATAGCCGGGATTGATGCTGATGGAAAGCTGAGAATATACACTCCAACTGGTTCAAAAGCTTGGATGTTGGATCCATCAGAAGTGCAACTTGTGGAGGAAGAGGAGCTTCATATTGGAGACTGGGTTAGGGTTAAAACATCAATATCAACCCCAACCCACCAATGGGGAGAGGTGAATCGTTCGAGCGTTGGGGTGGTGCATCGGATGGAAAATGAGGAGCTTTGGGTGGCCTTTTGCTTCACGGAGAGGTTGTGGCTTTGCAAGGCATTGGAAATGGAACGGGTCAGGCCCTTTAGAGTGGGGGACAAAGTGAGGATTAGAGAAGGGTTGGTGTCCCCACGGTGGGGTTGGGGAATGGAGACGCATGCCAGCAAAGGAGAGGTAGTTGGGGTGGATGCAAATGGAAAGTTAAGAATTAAGTTCCGGTGGAGAGAAGGGAGACCATGGATTGGAGATCCTGCTGATGTCACTCTTGATGAAAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Pkinase zf-C3HC4 zf-RING_2 zf-RING_5

Protein Analysis

1363

Amino Acids

150.61

Weight (kDa)

5.8

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 3 - 159 1.6e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 3 - 159 1.4e-15 Protein kinase domain
Ank_2 PF12796 227 - 309 7.1e-13 Ankyrin repeats (3 copies)
Ank PF00023 246 - 279 5.3e-06 Ankyrin repeat
Ank_2 PF12796 321 - 401 9.9e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 321 - 365 8.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 440 - 525 7.3e-14 Ankyrin repeats (3 copies)
Ank PF00023 461 - 492 1.6e-06 Ankyrin repeat
Ank_4 PF13637 466 - 515 3e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 473 - 549 1.1e-07 Ankyrin repeats (3 copies)
SH3_15 PF18346 658 - 723 7.5e-14 Mind bomb SH3 repeat domain
SH3_15 PF18346 792 - 849 2.7e-08 Mind bomb SH3 repeat domain
SH3_15 PF18346 855 - 899 6.3e-06 Mind bomb SH3 repeat domain
SH3_15 PF18346 911 - 973 1.6e-07 Mind bomb SH3 repeat domain
SH3_15 PF18346 981 - 1101 2.9e-16 Mind bomb SH3 repeat domain
SH3_15 PF18346 1108 - 1229 2.1e-16 Mind bomb SH3 repeat domain
SH3_15 PF18346 1238 - 1357 1.6e-14 Mind bomb SH3 repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1563, 1982, 3224
AarI CACCTGC 1 cut(s) 3081
Acc36I ACCTGC 1 cut(s) 3081
AccB1I GGYRCC 1 cut(s) 2881
AccB7I CCANNNNNTGG 6 cut(s) 306, 311, 2048, 2145, 2662, 4052
AccI GTMKAC 3 cut(s) 766, 1082, 2284
AccII CGCG 2 cut(s) 511, 2800
AcoI YGGCCR 6 cut(s) 739, 979, 1413, 1509, 3142, 3523
AcsI RAATTY 6 cut(s) 330, 1141, 1826, 2183, 2502, 2769
AcuI CTGAAG 4 cut(s) 1770, 2359, 2580, 2683
AcvI CACGTG 1 cut(s) 2069
AcyI GRCGYC 1 cut(s) 1157
AfaI GTAC 9 cut(s) 151, 274, 440, 1056, 1390, 2083, 2601, 2961, 3045
AflII CTTAAG 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 2116
AgeI ACCGGT 1 cut(s) 293
AhlI ACTAGT 1 cut(s) 3227
AjiI CACGTC 1 cut(s) 770
AjnI CCWGG 9 cut(s) 2128, 2257, 2490, 2884, 2914, 2976, 3265, 3274, 3382
AjuI GAANNNNNNNTTGG 4 cut(s) 735, 767, 2175, 2207
AleI CACNNNNGTG 3 cut(s) 1170, 2451, 2732
Alw21I GWGCWC 3 cut(s) 293, 323, 1096
Alw26I GTCTC 6 cut(s) 717, 1797, 2435, 3714, 3956, 4036
Alw44I GTGCAC 2 cut(s) 289, 1092
Ama87I CYCGRG 2 cut(s) 1734, 3213
ApaLI GTGCAC 2 cut(s) 289, 1092
ApoI RAATTY 6 cut(s) 330, 1141, 1826, 2183, 2502, 2769
AseI ATTAAT 1 cut(s) 1773
AsiGI ACCGGT 1 cut(s) 293
Asp700I GAANNNNTTC 3 cut(s) 215, 1728, 3123
AspA2I CCTAGG 2 cut(s) 401, 2420
AspS9I GGNCC 4 cut(s) 947, 3380, 3482, 3891
AsuC2I CCSGG 3 cut(s) 1735, 1736, 3610
AsuHPI GGTGA 9 cut(s) 208, 437, 1444, 2396, 2450, 2687, 2963, 3352, 3787
AsuNHI GCTAGC 1 cut(s) 475
AvaI CYCGRG 2 cut(s) 1734, 3213
AvaII GGWCC 1 cut(s) 947
AvrII CCTAGG 2 cut(s) 401, 2420
BaeGI GKGCMC 2 cut(s) 293, 1096
BalI TGGCCA 6 cut(s) 741, 981, 1415, 1511, 3144, 3525
BamHI GGATCC 5 cut(s) 129, 557, 844, 875, 3673
BanI GGYRCC 1 cut(s) 2881
BanII GRGCYC 1 cut(s) 3202
BauI CACGAG 1 cut(s) 1959
BbrPI CACGTG 1 cut(s) 2069
BbsI GAAGAC 1 cut(s) 1758
Bbv12I GWGCWC 3 cut(s) 293, 323, 1096
BceAI ACGGC 1 cut(s) 1419
BciT130I CCWGG 9 cut(s) 2130, 2259, 2492, 2886, 2916, 2978, 3267, 3276, 3384
BciVI GTATCC 2 cut(s) 595, 2120
BcnI CCSGG 3 cut(s) 1735, 1736, 3610
BcoDI GTCTC 6 cut(s) 717, 1797, 2435, 3714, 3956, 4036
BcuI ACTAGT 1 cut(s) 3227
BfaI CTAG 7 cut(s) 402, 476, 525, 866, 2421, 2634, 3228
BfmI CTRYAG 7 cut(s) 243, 434, 681, 763, 1120, 2160, 3603
BfrI CTTAAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 3081
BfuI GTATCC 2 cut(s) 595, 2120
BglI GCCNNNNNGGC 1 cut(s) 1517
BglII AGATCT 2 cut(s) 664, 923
BlnI CCTAGG 2 cut(s) 401, 2420
Bme18I GGWCC 1 cut(s) 947
BmeT110I CYCGRG 2 cut(s) 1734, 3213
BmgBI CACGTC 1 cut(s) 770
BmgT120I GGNCC 4 cut(s) 947, 3380, 3482, 3891
BmrI ACTGGG 3 cut(s) 2209, 2965, 3733
BmtI GCTAGC 1 cut(s) 479
BmuI ACTGGG 3 cut(s) 2209, 2965, 3733
BoxI GACNNNNGTC 1 cut(s) 634
BpiI GAAGAC 1 cut(s) 1758
BplI GAGNNNNNCTC 2 cut(s) 3184, 3216
BpmI CTGGAG 7 cut(s) 1035, 1371, 1572, 1962, 2122, 2151, 2898
Bpu10I CCTNAGC 1 cut(s) 1368
BpuEI CTTGAG 2 cut(s) 109, 3083
BpuMI CCSGG 3 cut(s) 1735, 1736, 3610
BsaAI YACGTR 3 cut(s) 1628, 2069, 2117
BsaBI GATNNNNATC 2 cut(s) 843, 2245
BsaHI GRCGYC 1 cut(s) 1157
BsaI GGTCTC 2 cut(s) 2435, 4036
BsaWI WCCGGW 2 cut(s) 293, 4026
Bse118I RCCGGY 2 cut(s) 193, 293
Bse1I ACTGG 7 cut(s) 1262, 1512, 2204, 2960, 3005, 3655, 3728
Bse3DI GCAATG 2 cut(s) 1160, 1706
Bse8I GATNNNNATC 2 cut(s) 843, 2245
BseBI CCWGG 9 cut(s) 2130, 2259, 2492, 2886, 2916, 2978, 3267, 3276, 3384
BseJI GATNNNNATC 2 cut(s) 843, 2245
BseMI GCAATG 2 cut(s) 1160, 1706
BseMII CTCAG 2 cut(s) 1382, 3623
BseNI ACTGG 7 cut(s) 1262, 1512, 2204, 2960, 3005, 3655, 3728
BseRI GAGGAG 4 cut(s) 2366, 3303, 3719, 3833
BseSI GKGCMC 2 cut(s) 293, 1096
BseYI CCCAGC 1 cut(s) 1377
BsgI GTGCAG 2 cut(s) 798, 1257
Bsh1236I CGCG 2 cut(s) 511, 2800
BshNI GGYRCC 1 cut(s) 2881
BshTI ACCGGT 1 cut(s) 293
BsiHKAI GWGCWC 3 cut(s) 293, 323, 1096
BsiHKCI CYCGRG 2 cut(s) 1734, 3213
BsiSI CCGG 5 cut(s) 194, 294, 1735, 3609, 4027
BslFI GGGAC 7 cut(s) 933, 1598, 1889, 2210, 2775, 3920, 3922
BsmAI GTCTC 6 cut(s) 717, 1797, 2435, 3714, 3956, 4036
BsmBI CGTCTC 2 cut(s) 717, 3956
BsmFI GGGAC 7 cut(s) 933, 1598, 1889, 2210, 2775, 3920, 3922
Bso31I GGTCTC 2 cut(s) 2435, 4036
BsoBI CYCGRG 2 cut(s) 1734, 3213
Bsp1286I GDGCHC 4 cut(s) 293, 323, 1096, 3202
Bsp1407I TGTACA 3 cut(s) 149, 272, 3043
Bsp19I CCATGG 3 cut(s) 305, 2333, 4046
BspCNI CTCAG 2 cut(s) 1381, 3624
BspFNI CGCG 2 cut(s) 511, 2800
BspHI TCATGA 2 cut(s) 1306, 1951
BspMAI CTGCAG 4 cut(s) 247, 438, 685, 2164
BspMI ACCTGC 1 cut(s) 3081
BspOI GCTAGC 1 cut(s) 479
BspQI GCTCTTC 2 cut(s) 756, 1734
BspT107I GGYRCC 1 cut(s) 2881
BspTI CTTAAG 1 cut(s) 80
BspTNI GGTCTC 2 cut(s) 2435, 4036
BsrDI GCAATG 2 cut(s) 1160, 1706
BsrFI RCCGGY 2 cut(s) 193, 293
BsrGI TGTACA 3 cut(s) 149, 272, 3043
BsrI ACTGG 7 cut(s) 1262, 1512, 2204, 2960, 3005, 3655, 3728
BssAI RCCGGY 2 cut(s) 193, 293
BssNAI GTATAC 2 cut(s) 1083, 2285
BssNI GRCGYC 1 cut(s) 1157
BssSI CACGAG 1 cut(s) 1959
BssT1I CCWWGG 6 cut(s) 305, 401, 2042, 2333, 2420, 4046
Bst1107I GTATAC 2 cut(s) 1083, 2285
Bst2BI CACGAG 1 cut(s) 1959
Bst2UI CCWGG 9 cut(s) 2130, 2259, 2492, 2886, 2916, 2978, 3267, 3276, 3384
Bst4CI ACNGT 9 cut(s) 701, 1135, 1278, 1697, 2086, 2833, 3008, 3041, 3945
Bst6I CTCTTC 5 cut(s) 756, 1224, 1315, 1734, 3696
BstACI GRCGYC 1 cut(s) 1157
BstAFI CTTAAG 1 cut(s) 80
BstAPI GCANNNNNTGC 1 cut(s) 1610
BstAUI TGTACA 3 cut(s) 149, 272, 3043
BstBAI YACGTR 3 cut(s) 1628, 2069, 2117
BstC8I GCNNGC 9 cut(s) 434, 477, 983, 1344, 2529, 2708, 3073, 3969, 3973
BstDEI CTNAG 5 cut(s) 158, 1368, 1658, 1966, 3632
BstDSI CCRYGG 5 cut(s) 305, 509, 2333, 3941, 4046
BstENI CCTNNNNNAGG 1 cut(s) 1760
BstFNI CGCG 2 cut(s) 511, 2800
BstMAI GTCTC 6 cut(s) 717, 1797, 2435, 3714, 3956, 4036
BstNI CCWGG 9 cut(s) 2130, 2259, 2492, 2886, 2916, 2978, 3267, 3276, 3384
BstNSI RCATGY 2 cut(s) 2015, 3971
BstPAI GACNNNNGTC 1 cut(s) 634
BstSFI CTRYAG 7 cut(s) 243, 434, 681, 763, 1120, 2160, 3603
BstSLI GKGCMC 2 cut(s) 293, 1096
BstUI CGCG 2 cut(s) 511, 2800
BstV2I GAAGAC 1 cut(s) 1758
BstXI CCANNNNNNTGG 5 cut(s) 186, 2334, 2456, 2523, 3282
BstZ17I GTATAC 2 cut(s) 1083, 2285
BsuI GTATCC 2 cut(s) 595, 2120
BtgI CCRYGG 5 cut(s) 305, 509, 2333, 3941, 4046
BtgZI GCGATG 1 cut(s) 561
BtrI CACGTC 1 cut(s) 770
BtsI GCAGTG 1 cut(s) 1269
BtsIMutI CAGTG 7 cut(s) 206, 706, 1269, 1274, 1505, 1702, 3046
BveI ACCTGC 1 cut(s) 3081
Cac8I GCNNGC 9 cut(s) 434, 477, 983, 1344, 2529, 2708, 3073, 3969, 3973
CciI TCATGA 2 cut(s) 1306, 1951
Cfr10I RCCGGY 2 cut(s) 193, 293
Cfr13I GGNCC 4 cut(s) 947, 3380, 3482, 3891
Cfr42I CCGCGG 1 cut(s) 512
Cfr9I CCCGGG 1 cut(s) 1734
CseI GACGC 3 cut(s) 1165, 1250, 3973
Csp6I GTAC 9 cut(s) 150, 273, 439, 1055, 1389, 2082, 2600, 2960, 3044
CspAI ACCGGT 1 cut(s) 293
CviQI GTAC 9 cut(s) 150, 273, 439, 1055, 1389, 2082, 2600, 2960, 3044
DdeI CTNAG 5 cut(s) 158, 1368, 1658, 1966, 3632
DraI TTTAAA 2 cut(s) 1831, 2871
EaeI YGGCCR 6 cut(s) 739, 979, 1413, 1509, 3142, 3523
Eam1104I CTCTTC 5 cut(s) 756, 1224, 1315, 1734, 3696
EarI CTCTTC 5 cut(s) 756, 1224, 1315, 1734, 3696
EciI GGCGGA 1 cut(s) 479
Eco130I CCWWGG 6 cut(s) 305, 401, 2042, 2333, 2420, 4046
Eco24I GRGCYC 1 cut(s) 3202
Eco31I GGTCTC 2 cut(s) 2435, 4036
Eco32I GATATC 1 cut(s) 2629
Eco47I GGWCC 1 cut(s) 947
Eco57I CTGAAG 4 cut(s) 1770, 2359, 2580, 2683
Eco72I CACGTG 1 cut(s) 2069
Eco88I CYCGRG 2 cut(s) 1734, 3213
EcoNI CCTNNNNNAGG 1 cut(s) 1760
EcoO109I RGGNCCY 1 cut(s) 3891
EcoRI GAATTC 1 cut(s) 2183
EcoRII CCWGG 9 cut(s) 2128, 2257, 2490, 2884, 2914, 2976, 3265, 3274, 3382
EcoRV GATATC 1 cut(s) 2629
EcoT14I CCWWGG 6 cut(s) 305, 401, 2042, 2333, 2420, 4046
EcoT22I ATGCAT 3 cut(s) 259, 999, 2431
EcoT38I GRGCYC 1 cut(s) 3202
ErhI CCWWGG 6 cut(s) 305, 401, 2042, 2333, 2420, 4046
Esp3I CGTCTC 2 cut(s) 717, 3956
FalI AAGNNNNNCTT 4 cut(s) 3675, 3707, 3693, 3725
FaqI GGGAC 7 cut(s) 933, 1598, 1889, 2210, 2775, 3920, 3922
FauI CCCGC 4 cut(s) 679, 927, 1444, 3452
FauNDI CATATG 1 cut(s) 1855
FblI GTMKAC 3 cut(s) 766, 1082, 2284
FriOI GRGCYC 1 cut(s) 3202
FspBI CTAG 7 cut(s) 402, 476, 525, 866, 2421, 2634, 3228
GsaI CCCAGC 1 cut(s) 1381
GsuI CTGGAG 7 cut(s) 1035, 1371, 1572, 1962, 2122, 2151, 2898
HapII CCGG 5 cut(s) 194, 294, 1735, 3609, 4027
HgaI GACGC 3 cut(s) 1165, 1250, 3973
Hin1I GRCGYC 1 cut(s) 1157
HindIII AAGCTT 3 cut(s) 550, 2592, 3660
HpaII CCGG 5 cut(s) 194, 294, 1735, 3609, 4027
HphI GGTGA 9 cut(s) 208, 437, 1444, 2396, 2450, 2687, 2963, 3352, 3787
Hpy99I CGWCG 1 cut(s) 1244
HpyCH4III ACNGT 9 cut(s) 701, 1135, 1278, 1697, 2086, 2833, 3008, 3041, 3945
HpyCH4IV ACGT 8 cut(s) 540, 769, 1387, 1422, 1627, 2068, 2116, 3114
HpyF3I CTNAG 5 cut(s) 158, 1368, 1658, 1966, 3632
HpySE526I ACGT 8 cut(s) 540, 769, 1387, 1422, 1627, 2068, 2116, 3114
Hsp92I GRCGYC 1 cut(s) 1157
KspI CCGCGG 1 cut(s) 512
LguI GCTCTTC 2 cut(s) 756, 1734
MaeI CTAG 7 cut(s) 402, 476, 525, 866, 2421, 2634, 3228
MaeII ACGT 8 cut(s) 540, 769, 1387, 1422, 1627, 2068, 2116, 3114
MfeI CAATTG 1 cut(s) 1245
MhlI GDGCHC 4 cut(s) 293, 323, 1096, 3202
MlsI TGGCCA 6 cut(s) 741, 981, 1415, 1511, 3144, 3525
MluNI TGGCCA 6 cut(s) 741, 981, 1415, 1511, 3144, 3525
MlyI GAGTC 4 cut(s) 257, 2212, 2595, 2925
Mox20I TGGCCA 6 cut(s) 741, 981, 1415, 1511, 3144, 3525
Mph1103I ATGCAT 3 cut(s) 259, 999, 2431
MroXI GAANNNNTTC 3 cut(s) 215, 1728, 3123
MscI TGGCCA 6 cut(s) 741, 981, 1415, 1511, 3144, 3525
MslI CAYNNNNRTG 9 cut(s) 966, 1170, 1858, 1881, 2451, 2732, 3240, 3585, 3591
Msp20I TGGCCA 6 cut(s) 741, 981, 1415, 1511, 3144, 3525
MspA1I CMGCKG 3 cut(s) 511, 686, 1550
MspCI CTTAAG 1 cut(s) 80
MspI CCGG 5 cut(s) 194, 294, 1735, 3609, 4027
MunI CAATTG 1 cut(s) 1245
MvaI CCWGG 9 cut(s) 2130, 2259, 2492, 2886, 2916, 2978, 3267, 3276, 3384
MvnI CGCG 2 cut(s) 511, 2800
NciI CCSGG 3 cut(s) 1735, 1736, 3610
NcoI CCATGG 3 cut(s) 305, 2333, 4046
NdeI CATATG 1 cut(s) 1855
NheI GCTAGC 1 cut(s) 475
NmuCI GTSAC 8 cut(s) 116, 204, 692, 1712, 2384, 2725, 2951, 4069
NsiI ATGCAT 3 cut(s) 259, 999, 2431
NspI RCATGY 2 cut(s) 2015, 3971
OliI CACNNNNGTG 3 cut(s) 1170, 2451, 2732
PaeI GCATGC 1 cut(s) 3971
PaeR7I CTCGAG 1 cut(s) 3213
PagI TCATGA 2 cut(s) 1306, 1951
PaqCI CACCTGC 1 cut(s) 3081
PciSI GCTCTTC 2 cut(s) 756, 1734
PcsI WCGNNNNNNNCGW 1 cut(s) 103
PdmI GAANNNNTTC 3 cut(s) 215, 1728, 3123
PfeI GAWTC 6 cut(s) 4, 211, 1049, 3209, 3453, 3778
PflMI CCANNNNNTGG 6 cut(s) 306, 311, 2048, 2145, 2662, 4052
PfoI TCCNGGA 2 cut(s) 2128, 2914
PinAI ACCGGT 1 cut(s) 293
PleI GAGTC 4 cut(s) 256, 2212, 2594, 2925
PmaCI CACGTG 1 cut(s) 2069
PmlI CACGTG 1 cut(s) 2069
PpsI GAGTC 4 cut(s) 256, 2212, 2594, 2925
Ppu21I YACGTR 3 cut(s) 1628, 2069, 2117
PshAI GACNNNNGTC 1 cut(s) 634
PshBI ATTAAT 1 cut(s) 1773
PsiI TTATAA 3 cut(s) 1563, 1982, 3224
Psp6I CCWGG 9 cut(s) 2128, 2257, 2490, 2884, 2914, 2976, 3265, 3274, 3382
PspCI CACGTG 1 cut(s) 2069
PspFI CCCAGC 1 cut(s) 1377
PspGI CCWGG 9 cut(s) 2128, 2257, 2490, 2884, 2914, 2976, 3265, 3274, 3382
PspPI GGNCC 4 cut(s) 947, 3380, 3482, 3891
PstI CTGCAG 4 cut(s) 247, 438, 685, 2164
RsaI GTAC 9 cut(s) 151, 274, 440, 1056, 1390, 2083, 2601, 2961, 3045
RsaNI GTAC 9 cut(s) 150, 273, 439, 1055, 1389, 2082, 2600, 2960, 3044
RseI CAYNNNNRTG 9 cut(s) 966, 1170, 1858, 1881, 2451, 2732, 3240, 3585, 3591
SacII CCGCGG 1 cut(s) 512
SapI GCTCTTC 2 cut(s) 756, 1734
Sau96I GGNCC 4 cut(s) 947, 3380, 3482, 3891
SchI GAGTC 4 cut(s) 257, 2212, 2595, 2925
SduI GDGCHC 4 cut(s) 293, 323, 1096, 3202
SfcI CTRYAG 7 cut(s) 243, 434, 681, 763, 1120, 2160, 3603
Sfr274I CTCGAG 1 cut(s) 3213
Sfr303I CCGCGG 1 cut(s) 512
SgrBI CCGCGG 1 cut(s) 512
SinI GGWCC 1 cut(s) 947
SlaI CTCGAG 1 cut(s) 3213
SmaI CCCGGG 1 cut(s) 1736
SmiMI CAYNNNNRTG 9 cut(s) 966, 1170, 1858, 1881, 2451, 2732, 3240, 3585, 3591
SmlI CTYRAG 4 cut(s) 80, 124, 3062, 3213
SmoI CTYRAG 4 cut(s) 80, 124, 3062, 3213
SpeI ACTAGT 1 cut(s) 3227
SphI GCATGC 1 cut(s) 3971
SspI AATATT 1 cut(s) 1803
SspMI CTAG 7 cut(s) 402, 476, 525, 866, 2421, 2634, 3228
StyI CCWWGG 6 cut(s) 305, 401, 2042, 2333, 2420, 4046
TaaI ACNGT 9 cut(s) 701, 1135, 1278, 1697, 2086, 2833, 3008, 3041, 3945
TaiI ACGT 8 cut(s) 543, 772, 1390, 1425, 1630, 2071, 2119, 3117
TaqI TCGA 6 cut(s) 106, 355, 789, 2986, 3214, 3785
TatI WGTACW 5 cut(s) 149, 272, 438, 1054, 3043
TauI GCSGC 1 cut(s) 2800
TfiI GAWTC 6 cut(s) 4, 211, 1049, 3209, 3453, 3778
TscAI CASTG 7 cut(s) 213, 706, 1276, 1281, 1512, 1702, 3046
TseFI GTSAC 8 cut(s) 116, 204, 692, 1712, 2384, 2725, 2951, 4069
Tsp45I GTSAC 8 cut(s) 116, 204, 692, 1712, 2384, 2725, 2951, 4069
TspGWI ACGGA 3 cut(s) 2631, 3071, 3860
TspMI CCCGGG 1 cut(s) 1734
TspRI CASTG 7 cut(s) 213, 706, 1276, 1281, 1512, 1702, 3046
Van91I CCANNNNNTGG 6 cut(s) 306, 311, 2048, 2145, 2662, 4052
Vha464I CTTAAG 1 cut(s) 80
VneI GTGCAC 2 cut(s) 289, 1092
VpaK11BI GGWCC 1 cut(s) 947
VspI ATTAAT 1 cut(s) 1773
XagI CCTNNNNNAGG 1 cut(s) 1760
XapI RAATTY 6 cut(s) 330, 1141, 1826, 2183, 2502, 2769
XceI RCATGY 2 cut(s) 2015, 3971
XcmI CCANNNNNNNNNTGG 8 cut(s) 25, 2049, 2453, 2997, 3186, 3593, 3762, 3948
XhoI CTCGAG 1 cut(s) 3213
XmaI CCCGGG 1 cut(s) 1734
XmaJI CCTAGG 2 cut(s) 401, 2420
XmiI GTMKAC 3 cut(s) 766, 1082, 2284
XmnI GAANNNNTTC 3 cut(s) 215, 1728, 3123
XspI CTAG 7 cut(s) 402, 476, 525, 866, 2421, 2634, 3228
Zsp2I ATGCAT 3 cut(s) 259, 999, 2431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.