Rh1DG345800

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
N/A
Physical Location & Seq
Forward (+)
56955153 .. 56955608
456 bp
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UTR
Exon/CDS
Intron
Rh1DG345800.1

Sequence Viewer

Length: 456 bp
ATGGCGTCGGACGTTGCTGAATTTCTGTGGAGCAAGTTCGTGAGCTCTCTTGAAGAGGCAGAGACTGAATTATCAGGTTCTATTTCAATCAAGGATGTTATTTCTCACTTCAAAGCAATCAGAGATGGTGTGAAACTGATCGATATAATGCCGGAGCGATATTCCTTCTCGAAGAACATCCCTCTTCTCTACGAGCTCAACGACGCCTTGGCCGAGTATCGAATCTTCGCGAATGAGTGCAAGGAGCTTAATAAGAAGCTGCTGCTCTTCAACCCTGTAAAACATTACTTCATCAGAAGTATGAAGGGTCGGCTCATTAGAATCAAACAGAAGCTTGAGAAAAAGGAGATTCCCCACAAAAAAAGAAAAGAGGGAGGCTCCGCGACAAGAGCTTCGCAAGCCCATGAAGAAAGTAGAGCTGAGGACTACACTCTCGTCAAACGTTTTCGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

151

Amino Acids

17.59

Weight (kDa)

9.47

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 230, 383
AciI CCGC 1 cut(s) 381
AclI AACGTT 1 cut(s) 442
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 1 cut(s) 20
AcyI GRCGYC 2 cut(s) 5, 204
AgsI TTSAA 4 cut(s) 53, 87, 112, 271
AluBI AGCT 7 cut(s) 45, 196, 247, 259, 334, 392, 419
AluI AGCT 7 cut(s) 45, 196, 247, 259, 334, 392, 419
Alw21I GWGCWC 2 cut(s) 47, 198
Alw26I GTCTC 1 cut(s) 56
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 1 cut(s) 210
ApeKI GCWGC 2 cut(s) 259, 262
ApoI RAATTY 1 cut(s) 20
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
BanII GRGCYC 2 cut(s) 47, 198
BarI GAAGNNNNNNTAC 2 cut(s) 209, 241
Bbv12I GWGCWC 2 cut(s) 47, 198
BbvCI CCTCAGC 1 cut(s) 420
BbvI GCAGC 2 cut(s) 246, 249
BccI CCATC 1 cut(s) 119
BcoDI GTCTC 1 cut(s) 56
BisI GCNGC 2 cut(s) 260, 263
BlsI GCNGC 2 cut(s) 261, 264
BmiI GGNNCC 1 cut(s) 379
BplI GAGNNNNNCTC 2 cut(s) 362, 394
Bpu10I CCTNAGC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 356
Bsa29I ATCGAT 1 cut(s) 141
BsaHI GRCGYC 2 cut(s) 5, 204
BsaJI CCNNGG 1 cut(s) 207
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 207
BseGI GGATG 2 cut(s) 100, 177
BseMII CTCAG 1 cut(s) 411
BseXI GCAGC 2 cut(s) 246, 249
Bsh1236I CGCG 2 cut(s) 230, 383
BshFI GGCC 1 cut(s) 212
BshVI ATCGAT 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 47, 198
BsiSI CCGG 1 cut(s) 152
BsmAI GTCTC 1 cut(s) 56
BsnI GGCC 1 cut(s) 212
Bsp1286I GDGCHC 2 cut(s) 47, 198
Bsp143I GATC 2 cut(s) 138, 450
Bsp68I TCGCGA 1 cut(s) 230
BspACI CCGC 1 cut(s) 381
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 1 cut(s) 412
BspDI ATCGAT 1 cut(s) 141
BspFNI CGCG 2 cut(s) 230, 383
BspLI GGNNCC 1 cut(s) 379
BspQI GCTCTTC 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 207
BssMI GATC 2 cut(s) 138, 450
BssNI GRCGYC 2 cut(s) 5, 204
BssT1I CCWWGG 1 cut(s) 207
Bst6I CTCTTC 3 cut(s) 48, 189, 272
BstACI GRCGYC 2 cut(s) 5, 204
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 1 cut(s) 420
BstF5I GGATG 2 cut(s) 100, 177
BstFNI CGCG 2 cut(s) 230, 383
BstKTI GATC 2 cut(s) 141, 453
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 2 cut(s) 138, 450
BstMWI GCNNNNNNNGC 2 cut(s) 389, 398
BstUI CGCG 2 cut(s) 230, 383
BstV1I GCAGC 2 cut(s) 246, 249
BstX2I RGATCY 1 cut(s) 450
BstYI RGATCY 1 cut(s) 450
Bsu15I ATCGAT 1 cut(s) 141
BsuRI GGCC 1 cut(s) 212
BsuTUI ATCGAT 1 cut(s) 141
BtsCI GGATG 2 cut(s) 100, 177
BtuMI TCGCGA 1 cut(s) 230
Cac8I GCNNGC 1 cut(s) 399
CaiI CAGNNNCTG 1 cut(s) 65
ClaI ATCGAT 1 cut(s) 141
CseI GACGC 1 cut(s) 212
CviAII CATG 1 cut(s) 404
DdeI CTNAG 1 cut(s) 420
DpnI GATC 2 cut(s) 140, 452
DpnII GATC 2 cut(s) 138, 450
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 3 cut(s) 48, 189, 272
EarI CTCTTC 3 cut(s) 48, 189, 272
Ecl136II GAGCTC 2 cut(s) 45, 196
Eco130I CCWWGG 1 cut(s) 207
Eco24I GRGCYC 2 cut(s) 47, 198
Eco53kI GAGCTC 2 cut(s) 45, 196
EcoICRI GAGCTC 2 cut(s) 45, 196
EcoT14I CCWWGG 1 cut(s) 207
EcoT38I GRGCYC 2 cut(s) 47, 198
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 1 cut(s) 407
FaiI YATR 3 cut(s) 146, 302, 405
FatI CATG 1 cut(s) 403
Fnu4HI GCNGC 2 cut(s) 260, 263
FokI GGATG 2 cut(s) 107, 164
FriOI GRGCYC 2 cut(s) 47, 198
Fsp4HI GCNGC 2 cut(s) 260, 263
GluI GCNGC 2 cut(s) 260, 263
HaeIII GGCC 1 cut(s) 212
HapII CCGG 1 cut(s) 152
HgaI GACGC 1 cut(s) 212
Hin1I GRCGYC 2 cut(s) 5, 204
Hin1II CATG 1 cut(s) 407
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 3 cut(s) 222, 321, 349
HpaII CCGG 1 cut(s) 152
Hpy188I TCNGA 5 cut(s) 10, 122, 296, 450, 455
Hpy188III TCNNGA 4 cut(s) 40, 50, 169, 229
Hpy99I CGWCG 2 cut(s) 10, 206
HpyAV CCTTC 2 cut(s) 175, 298
HpyCH4IV ACGT 2 cut(s) 12, 442
HpyCH4V TGCA 1 cut(s) 240
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 398
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 2 cut(s) 12, 442
Hsp92I GRCGYC 2 cut(s) 5, 204
Hsp92II CATG 1 cut(s) 407
Kzo9I GATC 2 cut(s) 138, 450
LguI GCTCTTC 1 cut(s) 272
LmnI GCTCC 4 cut(s) 30, 154, 244, 383
LpnPI CCDG 3 cut(s) 60, 165, 288
Lsp1109I GCAGC 2 cut(s) 246, 249
MaeII ACGT 2 cut(s) 12, 442
MalI GATC 2 cut(s) 140, 452
MboI GATC 2 cut(s) 138, 450
MboII GAAGA 6 cut(s) 65, 176, 184, 217, 259, 419
MflI RGATCY 1 cut(s) 450
MhlI GDGCHC 2 cut(s) 47, 198
MluCI AATT 2 cut(s) 20, 68
MnlI CCTC 5 cut(s) 49, 192, 364, 368, 415
MseI TTAA 1 cut(s) 249
MspI CCGG 1 cut(s) 152
MvnI CGCG 2 cut(s) 230, 383
MwoI GCNNNNNNNGC 2 cut(s) 389, 398
NdeII GATC 2 cut(s) 138, 450
NlaIII CATG 1 cut(s) 407
NlaIV GGNNCC 1 cut(s) 379
NmeAIII GCCGAG 1 cut(s) 238
NruI TCGCGA 1 cut(s) 230
PciSI GCTCTTC 1 cut(s) 272
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 210
PfeI GAWTC 3 cut(s) 222, 321, 349
PkrI GCNGC 2 cut(s) 261, 264
Psp124BI GAGCTC 2 cut(s) 47, 198
Psp1406I AACGTT 1 cut(s) 442
PspN4I GGNNCC 1 cut(s) 379
PstNI CAGNNNCTG 1 cut(s) 65
PsuI RGATCY 1 cut(s) 450
RruI TCGCGA 1 cut(s) 230
SacI GAGCTC 2 cut(s) 47, 198
SapI GCTCTTC 1 cut(s) 272
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 2 cut(s) 260, 263
Sau3AI GATC 2 cut(s) 138, 450
SduI GDGCHC 2 cut(s) 47, 198
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 2 cut(s) 20, 68
SsiI CCGC 1 cut(s) 381
SstI GAGCTC 2 cut(s) 47, 198
StyI CCWWGG 1 cut(s) 207
TaiI ACGT 2 cut(s) 15, 445
TaqI TCGA 3 cut(s) 141, 170, 220
TasI AATT 2 cut(s) 20, 68
TfiI GAWTC 3 cut(s) 222, 321, 349
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TseI GCWGC 2 cut(s) 259, 262
TspDTI ATGAA 3 cut(s) 280, 317, 420
XapI RAATTY 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.