Rh2AG170600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
N/A
Physical Location & Seq
Forward (+)
16198193 .. 16217131
18939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG170600.1

Sequence Viewer

Length: 1593 bp
ATGGCTTCTGAGGGCGCTACGAAATCGAGCTCAGCGGCCCTCGGCTCCGTCGTCGTGGTTGGCTCCGCTGGAGCCGTGGCATTGATCGGAGGAACTGGTTTGCCAGTAACATTTTGGACGCTGTGTGATTCTGTTAAGCAAATGAAGGATATACTGATGTTCAAACCCCAACTAAGGGATATCAAATCCACTTTGGACTCTTTGGAACCACTGATCAAAGAGATGGCAGAGTGTGAGAAGGAATTGGATGGTCAAGAGGAGGAGCTAGAGAATCTTAGAGTGGAAATGGAGGGAGGAGTGGAACTTATCCGCAAGTGCTCGAAAGCAAGCAGGTTCAAGCAGTATAAGTACGGCAGCAGGCTTGTTGAATTCAGCAAGTCACTTCAGAGGCTGTTGGGGATATTGAAAGTGCAGGGAATAAGGGATGTGAAGAAGGCATTGGTTTCGGTGAGAAATATCGAGAATGTGGTGCATAGAATTGAAGGAAATTGTGTGACACAGAACAATCAGTCTGAAAAAATTGCGGGGTGGTGTGCTGTGCCTGAGCCTCCAACGATTACTGTTGGATTGGATGTGCCTTTGTGGGAATTGAAGAAGAAGCTTTTTACTGACGGAGTATCGATGGTGATAATTACTGCTCCTGGAGGATGTGGAAAAACTACATTGGCAACAAAGTTTTGCAAGGATGCAGAAGTCAAAGTTATTATGGAAGTGGTTACCACAGTTGTAGTGGCTGCTCTAGGAGTAGCGTTCCAGCAGCTCTTTGATAGTGTTATAGAGGCTAACAGAAAAGCTACAATGTTTGATACCGTCTTCAGGGATCTCGATCTCACGCTCCAGTCTTATCAAGAGTTGGTTAAAGGTATAGAACAAGTTGAAAATTTTCAACCCTTGCCGGGCCTGCTAGATGCTTTAAAAACGAAGATGGAGGAGGGCAGTCAGCTTGTTGACAAGTGCTCCAATATTCATCCCTCGTTGAAGATTAAATTGTTGAAAAAGGCGAAATATGTTGGAAAACTTCGTGCATTGGATGGATCTCTTAAACAACACTTCCACATATTGAAATGGCGAGTAGACATCAGTCTGGTGAACAAGGCGAGCAATTTGATTGGTAGGAAACGAACTGAAAATCAAATCAGCGGGTGCGTGGTACCCAATCCTCCGTCATTTACTGTTGGATTGGATAAGCCTTTGAAGGATTTGAAGATGAAGTTTCTAAATGATGATCAAGTGTCAATGCTTGTGCTCACTGCTCCTGGAGGATGGGGGAAAACCACTTTGGCTCAGATGTTCTGTCGAGATGAGGAAGTCAAAGAGTTAGGTGGTGTTTGTAGACTTGTAGTTATTATGGAAGTGGTTATTACTGCAGTTACCACAATTGTAGGGGCTGCTCTAGGAGCAGTGTTCCAGCAGCTCTTTGATGGTGTTATAAAGGCTAACAGAAAAGCTACAAAGTGTAATTCCATCTTCAGGGATCTCAATCTCACGCTCCAGTCTTATCAAGAATTGGTTAAAGATATAGAACAAGTTGAAAATTTTCAACCATTGCAGGGCCTAGATGGTTTCAAAACGAAGATGGAGGAGGGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

530

Amino Acids

58.35

Weight (kDa)

8.55

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 28 - 152 2.5e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 245 - 353 1.9e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 460 - 513 2.3e-09 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1430
Acc36I ACCTGC 1 cut(s) 321
Acc65I GGTACC 1 cut(s) 1150
AccB1I GGYRCC 1 cut(s) 1150
AccI GTMKAC 2 cut(s) 1074, 1333
AciI CCGC 5 cut(s) 35, 66, 310, 524, 1140
AclWI GGATC 3 cut(s) 828, 1042, 1482
AcsI RAATTY 3 cut(s) 368, 880, 1534
AcuI CTGAAG 3 cut(s) 368, 799, 1453
AfaI GTAC 2 cut(s) 350, 1152
AfiI CCNNNNNNNGG 6 cut(s) 174, 175, 816, 896, 1470, 1550
AjnI CCWGG 2 cut(s) 640, 1255
AjuI GAANNNNNNNTTGG 4 cut(s) 422, 454, 1262, 1294
AluBI AGCT 8 cut(s) 30, 265, 601, 760, 794, 943, 1414, 1448
AluI AGCT 8 cut(s) 30, 265, 601, 760, 794, 943, 1414, 1448
Alw21I GWGCWC 4 cut(s) 32, 320, 959, 1248
AlwI GGATC 3 cut(s) 828, 1042, 1482
AlwNI CAGNNNCTG 1 cut(s) 391
AoxI GGCC 3 cut(s) 36, 898, 1552
ApeKI GCWGC 5 cut(s) 354, 734, 757, 1388, 1411
ApoI RAATTY 3 cut(s) 368, 880, 1534
Asp700I GAANNNNTTC 2 cut(s) 882, 1536
Asp718I GGTACC 1 cut(s) 1150
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 3 cut(s) 37, 898, 1552
AsuC2I CCSGG 1 cut(s) 897
AsuHPI GGTGA 3 cut(s) 460, 637, 1099
BanI GGYRCC 1 cut(s) 1150
BanII GRGCYC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 805
Bbv12I GWGCWC 4 cut(s) 32, 320, 959, 1248
BbvI GCAGC 5 cut(s) 366, 721, 769, 1375, 1423
BceAI ACGGC 2 cut(s) 59, 367
BcgI CGANNNNNNTGC 2 cut(s) 426, 460
BciT130I CCWGG 2 cut(s) 642, 1257
BclI TGATCA 2 cut(s) 213, 1225
BcnI CCSGG 1 cut(s) 897
BfaI CTAG 5 cut(s) 266, 740, 905, 1394, 1556
BfmI CTRYAG 1 cut(s) 1365
BfoI RGCGCY 1 cut(s) 18
BfuAI ACCTGC 1 cut(s) 321
BisI GCNGC 6 cut(s) 36, 355, 735, 758, 1389, 1412
BlpI GCTNAGC 1 cut(s) 31
BlsI GCNGC 6 cut(s) 37, 356, 736, 759, 1390, 1413
Bme1390I CCNGG 3 cut(s) 642, 897, 1257
BmgT120I GGNCC 3 cut(s) 37, 898, 1552
BmiI GGNNCC 5 cut(s) 46, 64, 73, 207, 1152
BmrFI CCNGG 3 cut(s) 642, 897, 1257
BmsI GCATC 2 cut(s) 676, 898
BoxI GACNNNNGTC 1 cut(s) 1080
BpiI GAAGAC 1 cut(s) 805
BpmI CTGGAG 5 cut(s) 90, 663, 821, 1278, 1475
Bpu10I CCTNAGC 1 cut(s) 543
Bpu1102I GCTNAGC 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 897
Bsa29I ATCGAT 1 cut(s) 620
BsaBI GATNNNNATC 2 cut(s) 825, 1479
BsaJI CCNNGG 2 cut(s) 40, 75
BsaXI ACNNNNNCTCC 2 cut(s) 941, 971
Bsc4I CCNNNNNNNGG 6 cut(s) 174, 175, 816, 896, 1470, 1550
Bse1I ACTGG 4 cut(s) 100, 104, 838, 1492
Bse3DI GCAATG 1 cut(s) 1544
Bse8I GATNNNNATC 2 cut(s) 825, 1479
BseBI CCWGG 2 cut(s) 642, 1257
BseCI ATCGAT 1 cut(s) 620
BseDI CCNNGG 2 cut(s) 40, 75
BseGI GGATG 8 cut(s) 253, 430, 577, 653, 691, 967, 1036, 1268
BseJI GATNNNNATC 2 cut(s) 825, 1479
BseLI CCNNNNNNNGG 6 cut(s) 174, 175, 816, 896, 1470, 1550
BseMI GCAATG 1 cut(s) 1544
BseMII CTCAG 3 cut(s) 45, 534, 1298
BseNI ACTGG 4 cut(s) 100, 104, 838, 1492
BseRI GAGGAG 4 cut(s) 272, 275, 309, 944
BseXI GCAGC 5 cut(s) 366, 721, 769, 1375, 1423
BsgI GTGCAG 1 cut(s) 431
BshFI GGCC 3 cut(s) 38, 900, 1554
BshNI GGYRCC 1 cut(s) 1150
BshVI ATCGAT 1 cut(s) 620
BsiHKAI GWGCWC 4 cut(s) 32, 320, 959, 1248
BsiSI CCGG 1 cut(s) 896
BslI CCNNNNNNNGG 6 cut(s) 174, 175, 816, 896, 1470, 1550
BsnI GGCC 3 cut(s) 38, 900, 1554
Bsp1286I GDGCHC 4 cut(s) 32, 320, 959, 1248
Bsp143I GATC 7 cut(s) 84, 213, 820, 826, 1034, 1225, 1474
Bsp1720I GCTNAGC 1 cut(s) 31
BspACI CCGC 5 cut(s) 35, 66, 310, 524, 1140
BspANI GGCC 3 cut(s) 38, 900, 1554
BspCNI CTCAG 3 cut(s) 44, 535, 1297
BspDI ATCGAT 1 cut(s) 620
BspLI GGNNCC 5 cut(s) 46, 64, 73, 207, 1152
BspMAI CTGCAG 1 cut(s) 1369
BspMI ACCTGC 1 cut(s) 321
BspPI GGATC 3 cut(s) 828, 1042, 1482
BspT107I GGYRCC 1 cut(s) 1150
BsrDI GCAATG 1 cut(s) 1544
BsrI ACTGG 4 cut(s) 100, 104, 838, 1492
BssECI CCNNGG 2 cut(s) 40, 75
BssMI GATC 7 cut(s) 84, 213, 820, 826, 1034, 1225, 1474
Bst2UI CCWGG 2 cut(s) 642, 1257
Bst4CI ACNGT 4 cut(s) 562, 724, 811, 1174
BstC8I GCNNGC 4 cut(s) 328, 359, 902, 1099
BstDEI CTNAG 6 cut(s) 9, 31, 173, 275, 543, 1284
BstDSI CCRYGG 1 cut(s) 75
BstEII GGTNACC 1 cut(s) 715
BstF5I GGATG 8 cut(s) 253, 430, 577, 653, 691, 967, 1036, 1268
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 7 cut(s) 87, 216, 823, 829, 1037, 1228, 1477
BstMBI GATC 7 cut(s) 84, 213, 820, 826, 1034, 1225, 1474
BstMWI GCNNNNNNNGC 2 cut(s) 901, 1397
BstNI CCWGG 2 cut(s) 642, 1257
BstPAI GACNNNNGTC 1 cut(s) 1080
BstPI GGTNACC 1 cut(s) 715
BstSCI CCNGG 3 cut(s) 640, 895, 1255
BstSFI CTRYAG 1 cut(s) 1365
BstV1I GCAGC 5 cut(s) 366, 721, 769, 1375, 1423
BstV2I GAAGAC 1 cut(s) 805
BstX2I RGATCY 3 cut(s) 820, 1034, 1474
BstYI RGATCY 3 cut(s) 820, 1034, 1474
Bsu15I ATCGAT 1 cut(s) 620
BsuRI GGCC 3 cut(s) 38, 900, 1554
BsuTUI ATCGAT 1 cut(s) 620
BtgI CCRYGG 1 cut(s) 75
BtsCI GGATG 8 cut(s) 253, 430, 577, 653, 691, 967, 1036, 1268
BtsI GCAGTG 2 cut(s) 1248, 1407
BtsIMutI CAGTG 3 cut(s) 209, 1248, 1407
BveI ACCTGC 1 cut(s) 321
Cac8I GCNNGC 4 cut(s) 328, 359, 902, 1099
CaiI CAGNNNCTG 1 cut(s) 391
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 3 cut(s) 37, 898, 1552
ClaI ATCGAT 1 cut(s) 620
CseI GACGC 1 cut(s) 127
Csp6I GTAC 2 cut(s) 349, 1151
CviQI GTAC 2 cut(s) 349, 1151
DdeI CTNAG 6 cut(s) 9, 31, 173, 275, 543, 1284
DpnI GATC 7 cut(s) 86, 215, 822, 828, 1036, 1227, 1476
DpnII GATC 7 cut(s) 84, 213, 820, 826, 1034, 1225, 1474
DraI TTTAAA 1 cut(s) 915
Ecl136II GAGCTC 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 32
Eco32I GATATC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 3 cut(s) 368, 799, 1453
Eco91I GGTNACC 1 cut(s) 715
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 1552
EcoO65I GGTNACC 1 cut(s) 715
EcoRI GAATTC 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 640, 1255
EcoRV GATATC 1 cut(s) 181
EcoT38I GRGCYC 1 cut(s) 32
FauI CCCGC 2 cut(s) 517, 1133
FbaI TGATCA 2 cut(s) 213, 1225
FblI GTMKAC 2 cut(s) 1074, 1333
Fnu4HI GCNGC 6 cut(s) 36, 355, 735, 758, 1389, 1412
FokI GGATG 8 cut(s) 260, 437, 584, 660, 698, 954, 1043, 1275
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 6 cut(s) 36, 355, 735, 758, 1389, 1412
FspBI CTAG 5 cut(s) 266, 740, 905, 1394, 1556
GlaI GCGC 1 cut(s) 16
GluI GCNGC 6 cut(s) 36, 355, 735, 758, 1389, 1412
GsuI CTGGAG 5 cut(s) 90, 663, 821, 1278, 1475
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 3 cut(s) 38, 900, 1554
HapII CCGG 1 cut(s) 896
HgaI GACGC 1 cut(s) 127
HhaI GCGC 1 cut(s) 17
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HincII GTYRAC 1 cut(s) 949
HindII GTYRAC 1 cut(s) 949
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 3 cut(s) 128, 197, 271
HpaII CCGG 1 cut(s) 896
HphI GGTGA 3 cut(s) 460, 637, 1099
Hpy166II GTNNAC 4 cut(s) 949, 1075, 1090, 1334
Hpy188I TCNGA 5 cut(s) 10, 89, 387, 514, 1287
Hpy188III TCNNGA 6 cut(s) 254, 460, 824, 848, 1298, 1502
Hpy8I GTNNAC 4 cut(s) 949, 1075, 1090, 1334
Hpy99I CGWCG 2 cut(s) 53, 56
HpyAV CCTTC 5 cut(s) 139, 232, 427, 476, 1189
HpyCH4III ACNGT 4 cut(s) 562, 724, 811, 1174
HpyCH4V TGCA 7 cut(s) 412, 472, 681, 689, 1025, 1367, 1549
HpyF10VI GCNNNNNNNGC 2 cut(s) 901, 1397
HpyF3I CTNAG 6 cut(s) 9, 31, 173, 275, 543, 1284
HspAI GCGC 1 cut(s) 15
KpnI GGTACC 1 cut(s) 1154
Ksp22I TGATCA 2 cut(s) 213, 1225
Kzo9I GATC 7 cut(s) 84, 213, 820, 826, 1034, 1225, 1474
Lsp1109I GCAGC 5 cut(s) 366, 721, 769, 1375, 1423
LweI GCATC 2 cut(s) 676, 898
MaeI CTAG 5 cut(s) 266, 740, 905, 1394, 1556
MaeIII GTNAC 5 cut(s) 106, 378, 493, 715, 1369
MalI GATC 7 cut(s) 86, 215, 822, 828, 1036, 1227, 1476
MboI GATC 7 cut(s) 84, 213, 820, 826, 1034, 1225, 1474
MboII GAAGA 9 cut(s) 442, 604, 607, 805, 934, 991, 1216, 1459, 1585
MfeI CAATTG 1 cut(s) 1377
MflI RGATCY 3 cut(s) 820, 1034, 1474
MhlI GDGCHC 4 cut(s) 32, 320, 959, 1248
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 4 cut(s) 544, 575, 991, 1156
MroXI GAANNNNTTC 2 cut(s) 882, 1536
MseI TTAA 6 cut(s) 135, 858, 914, 984, 1041, 1512
MspA1I CMGCKG 3 cut(s) 35, 68, 1140
MspI CCGG 1 cut(s) 896
MspR9I CCNGG 3 cut(s) 642, 897, 1257
MunI CAATTG 1 cut(s) 1377
MvaI CCWGG 2 cut(s) 642, 1257
MwoI GCNNNNNNNGC 2 cut(s) 901, 1397
NciI CCSGG 1 cut(s) 897
NdeII GATC 7 cut(s) 84, 213, 820, 826, 1034, 1225, 1474
NlaIV GGNNCC 5 cut(s) 46, 64, 73, 207, 1152
NmeAIII GCCGAG 1 cut(s) 21
NmuCI GTSAC 2 cut(s) 378, 493
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PdmI GAANNNNTTC 2 cut(s) 882, 1536
PfeI GAWTC 2 cut(s) 128, 271
PfoI TCCNGGA 2 cut(s) 640, 1255
PkrI GCNGC 6 cut(s) 37, 356, 736, 759, 1390, 1413
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PshAI GACNNNNGTC 1 cut(s) 1080
PsiI TTATAA 1 cut(s) 1430
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 2 cut(s) 640, 1255
PspEI GGTNACC 1 cut(s) 715
PspGI CCWGG 2 cut(s) 640, 1255
PspN4I GGNNCC 5 cut(s) 46, 64, 73, 207, 1152
PspPI GGNCC 3 cut(s) 37, 898, 1552
PstI CTGCAG 1 cut(s) 1369
PstNI CAGNNNCTG 1 cut(s) 391
PsuI RGATCY 3 cut(s) 820, 1034, 1474
RsaI GTAC 2 cut(s) 350, 1152
RsaNI GTAC 2 cut(s) 349, 1151
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 6 cut(s) 135, 858, 914, 984, 1041, 1512
SatI GCNGC 6 cut(s) 36, 355, 735, 758, 1389, 1412
Sau3AI GATC 7 cut(s) 84, 213, 820, 826, 1034, 1225, 1474
Sau96I GGNCC 3 cut(s) 37, 898, 1552
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 3 cut(s) 642, 897, 1257
SduI GDGCHC 4 cut(s) 32, 320, 959, 1248
SfaNI GCATC 2 cut(s) 676, 898
SfcI CTRYAG 1 cut(s) 1365
SsiI CCGC 5 cut(s) 35, 66, 310, 524, 1140
SspI AATATT 1 cut(s) 964
SspMI CTAG 5 cut(s) 266, 740, 905, 1394, 1556
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 3 cut(s) 640, 895, 1255
TaaI ACNGT 4 cut(s) 562, 724, 811, 1174
TaqI TCGA 6 cut(s) 26, 320, 459, 620, 825, 1297
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 2 cut(s) 128, 271
Tru1I TTAA 6 cut(s) 135, 858, 914, 984, 1041, 1512
Tru9I TTAA 6 cut(s) 135, 858, 914, 984, 1041, 1512
TscAI CASTG 3 cut(s) 216, 1255, 1407
TseFI GTSAC 2 cut(s) 378, 493
TseI GCWGC 5 cut(s) 354, 734, 757, 1388, 1411
Tsp45I GTSAC 2 cut(s) 378, 493
TspDTI ATGAA 3 cut(s) 158, 956, 1223
TspGWI ACGGA 3 cut(s) 37, 627, 1152
TspRI CASTG 3 cut(s) 216, 1255, 1407
XapI RAATTY 3 cut(s) 368, 880, 1534
XcmI CCANNNNNNNNNTGG 2 cut(s) 111, 727
XmiI GTMKAC 2 cut(s) 1074, 1333
XmnI GAANNNNTTC 2 cut(s) 882, 1536
XspI CTAG 5 cut(s) 266, 740, 905, 1394, 1556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.