Rh2CG175400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
N/A
Physical Location & Seq
Forward (+)
16027131 .. 16030492
3362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG175400.1

Sequence Viewer

Length: 411 bp
ATGGAAGTGGTTATTACTGCAGTTACCACAATTGTAGGGGCTGCTCTAGGAGCAGCGTTCCAGCAGCTCTTTGATGGTGTAATAAAGGCAAACAGAAAAGCTACAAAGTTTAATTCCGTCTTCGGGGATCTCAATCTCACGCTCCAGTCTTATCAAGAATTGGTTAAAGATATAGAACAAGTTGAAAATTTTCAACCATTGCAGGGCCTAGATGGTTTCAAAACGAAGATGGAGGAGGGCATACAGCTTGTTGACAAGTGCTCCAATATTCATCCCTTGCAGTTGAAGATCAATTTCTTCAAAAAGAAGAAATACGTTGGAAAACTTCGTGCATTGGATGGATCTCTTAAACAACTCTTCCTCATATTGAAATGGCAAGTAGACAACAATCTGAGGAACAGGGATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

136

Amino Acids

15.4

Weight (kDa)

9.38

Isoelectric Point (pI)

27.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 11 - 129 1.2e-20 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 381
AclWI GGATC 2 cut(s) 135, 349
AcsI RAATTY 1 cut(s) 187
AfiI CCNNNNNNNGG 2 cut(s) 123, 203
AgsI TTSAA 6 cut(s) 185, 194, 220, 286, 301, 370
AluBI AGCT 3 cut(s) 67, 101, 247
AluI AGCT 3 cut(s) 67, 101, 247
Alw21I GWGCWC 1 cut(s) 263
AlwI GGATC 2 cut(s) 135, 349
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 3 cut(s) 41, 53, 64
ApoI RAATTY 1 cut(s) 187
Asp700I GAANNNNTTC 1 cut(s) 189
AspS9I GGNCC 1 cut(s) 205
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 263
BbvI GCAGC 3 cut(s) 28, 65, 76
BccI CCATC 4 cut(s) 68, 206, 223, 332
BciVI GTATCC 1 cut(s) 397
BfaI CTAG 2 cut(s) 47, 209
BfmI CTRYAG 1 cut(s) 18
BfuI GTATCC 1 cut(s) 397
BisI GCNGC 3 cut(s) 42, 54, 65
BlsI GCNGC 3 cut(s) 43, 55, 66
BmgT120I GGNCC 1 cut(s) 205
BpiI GAAGAC 1 cut(s) 112
BpmI CTGGAG 1 cut(s) 128
BsaBI GATNNNNATC 1 cut(s) 132
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 203
Bse1I ACTGG 1 cut(s) 145
Bse3DI GCAATG 1 cut(s) 197
Bse8I GATNNNNATC 1 cut(s) 132
BseGI GGATG 2 cut(s) 271, 343
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 123, 203
BseMI GCAATG 1 cut(s) 197
BseMII CTCAG 1 cut(s) 383
BseNI ACTGG 1 cut(s) 145
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 3 cut(s) 28, 65, 76
BshFI GGCC 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 263
BslI CCNNNNNNNGG 2 cut(s) 123, 203
BsnI GGCC 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 263
Bsp143I GATC 3 cut(s) 127, 288, 341
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 384
BspMAI CTGCAG 1 cut(s) 22
BspPI GGATC 2 cut(s) 135, 349
BsrDI GCAATG 1 cut(s) 197
BsrI ACTGG 1 cut(s) 145
BssMI GATC 3 cut(s) 127, 288, 341
Bst6I CTCTTC 1 cut(s) 362
BstDEI CTNAG 1 cut(s) 392
BstF5I GGATG 2 cut(s) 271, 343
BstKTI GATC 3 cut(s) 130, 291, 344
BstMBI GATC 3 cut(s) 127, 288, 341
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstSFI CTRYAG 1 cut(s) 18
BstV1I GCAGC 3 cut(s) 28, 65, 76
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 2 cut(s) 127, 341
BstYI RGATCY 2 cut(s) 127, 341
BsuI GTATCC 1 cut(s) 397
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 2 cut(s) 271, 343
Cfr13I GGNCC 1 cut(s) 205
CviAII CATG 1 cut(s) 408
CviJI RGCY 5 cut(s) 41, 67, 101, 207, 247
CviKI_1 RGCY 5 cut(s) 41, 67, 101, 207, 247
DdeI CTNAG 1 cut(s) 392
DpnI GATC 3 cut(s) 129, 290, 343
DpnII GATC 3 cut(s) 127, 288, 341
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
EcoO109I RGGNCCY 1 cut(s) 205
FaeI CATG 1 cut(s) 411
FaiI YATR 4 cut(s) 173, 242, 365, 409
FatI CATG 1 cut(s) 407
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 3 cut(s) 42, 54, 65
FokI GGATG 2 cut(s) 258, 350
Fsp4HI GCNGC 3 cut(s) 42, 54, 65
FspBI CTAG 2 cut(s) 47, 209
GluI GCNGC 3 cut(s) 42, 54, 65
GsuI CTGGAG 1 cut(s) 128
HaeIII GGCC 1 cut(s) 207
Hin1II CATG 1 cut(s) 411
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
Hpy166II GTNNAC 2 cut(s) 253, 382
Hpy188I TCNGA 1 cut(s) 393
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 2 cut(s) 253, 382
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 4 cut(s) 20, 202, 280, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 392
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 1 cut(s) 411
Kzo9I GATC 3 cut(s) 127, 288, 341
LmnI GCTCC 3 cut(s) 50, 147, 266
LpnPI CCDG 4 cut(s) 74, 158, 188, 385
Lsp1109I GCAGC 3 cut(s) 28, 65, 76
MaeI CTAG 2 cut(s) 47, 209
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 22
MalI GATC 3 cut(s) 129, 290, 343
MboI GATC 3 cut(s) 127, 288, 341
MboII GAAGA 6 cut(s) 112, 238, 289, 298, 319, 349
MfeI CAATTG 1 cut(s) 30
MflI RGATCY 2 cut(s) 127, 341
MhlI GDGCHC 1 cut(s) 263
MluCI AATT 5 cut(s) 30, 112, 158, 187, 292
MmeI TCCRAC 1 cut(s) 298
MnlI CCTC 4 cut(s) 226, 229, 371, 387
MroXI GAANNNNTTC 1 cut(s) 189
MseI TTAA 3 cut(s) 111, 165, 348
MunI CAATTG 1 cut(s) 30
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeII GATC 3 cut(s) 127, 288, 341
NlaIII CATG 1 cut(s) 411
PdmI GAANNNNTTC 1 cut(s) 189
PkrI GCNGC 3 cut(s) 43, 55, 66
PspPI GGNCC 1 cut(s) 205
PstI CTGCAG 1 cut(s) 22
PsuI RGATCY 2 cut(s) 127, 341
SaqAI TTAA 3 cut(s) 111, 165, 348
SatI GCNGC 3 cut(s) 42, 54, 65
Sau3AI GATC 3 cut(s) 127, 288, 341
Sau96I GGNCC 1 cut(s) 205
SduI GDGCHC 1 cut(s) 263
SetI ASST 4 cut(s) 69, 103, 249, 318
SfcI CTRYAG 1 cut(s) 18
Sse9I AATT 5 cut(s) 30, 112, 158, 187, 292
SspI AATATT 1 cut(s) 268
SspMI CTAG 2 cut(s) 47, 209
TaiI ACGT 1 cut(s) 318
TasI AATT 5 cut(s) 30, 112, 158, 187, 292
Tru1I TTAA 3 cut(s) 111, 165, 348
Tru9I TTAA 3 cut(s) 111, 165, 348
TseI GCWGC 3 cut(s) 41, 53, 64
TspDTI ATGAA 1 cut(s) 260
TspGWI ACGGA 1 cut(s) 106
XapI RAATTY 1 cut(s) 187
XmiI GTMKAC 1 cut(s) 381
XmnI GAANNNNTTC 1 cut(s) 189
XspI CTAG 2 cut(s) 47, 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.