Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Forward (+)

2536646 .. 2541590

Gene Structure 4945 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG036900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGATGAAGAAGAAGGTGACAAGAAATGTAGGCAAGTATGAGGTTGGGCGAACGATCGGCGAAGGAACCTTCGCCAAGGTTAAGTTCGCCAGGAACGCCGAGACCGGCGAGAGTGTTGCCGTGAAGGTTATGGCCAAAAGCACCATTCTCAAGCACAGAATGGTTGATCAGATTAAAAGAGAGATATCCATAATGAAGATTGTCAGGCATCCGAATATAGTGAGGTTGCATGAGGTTTTGGCTGGCCGGACAAAGATATATATAATTCTTGAGTTTGTAACCGGAGGAGAATTGTTTGACAAAATTGTTCATCAAGGAAGGCTTCAGGAAAATGAATCAAGGAAATACTTTCAGCAGCTTATAGATGCAGTGTCTCATTGTCACAGTAAGGGTGTATACCACAGAGACCTGAAGCCTGAAAACCTTCTCCTTGATTCTTATGGAAATTTGAAAGTTTCTGATTTTGGACTAAGTGCATTGCCCCAGCAAGGGGTCGGTCTTCTTCACACAACATGTGGAACCCCAAACTATGTTGCCCCTGAGGTGCTGGGCAATCAAGGTTATGATGGTGCAGCTGCTGATGTTTGGTCTTGTGGGGTCATCCTCTTTGTTTTAATGGCTGGATGCCTCCCATTTCATGAGACAAACCTTGCTACCTTATATGAAAAGATTAATGCAGCACAGTTCTCCTGCCCGTTTTGGTTCTCTCCAGGGGCAAATTCATTAATACATAAGATACTTGATCCCAATCCTAAAACTCGTATTCGGATTGATGGAATCAGAAAGGATCCTTGGTTTAGAAAACATTATGCGCCTGTTGAATATAGGGAAGATGAGGAAGTCAGTTTAGATGATGTTCGTGCAGTCTTTGAGGACATTGAGGATCAATATGCAGAAGAAAGAACAGATAATAAAGACGGCGGTCCTTTATTAATGAATGCATTTGAGATGATTACCCTCTCCCAAGGGCTAAATTTATCTGCTTTGTTCGACAGGAGACAGGATTGTATAAAACGACAAACTCGTTTTGTTTCCCGCAAACCAGCTAAAGTCATAATTTCAAATGTAGAAGCTGTTGCAGAATCAATGGGTCTCAAGGTCCATACACGGAATTTCAAGACAAGACTTGAAGGTATATCTGCAAATAAGGCTGGGCAATTTGCAGTTGTCCTCGAGGTAATTAAGCTTTATCTGCATGATATTTCAAGTATCTTTCCAGAGGATGCCTTGAATACAGTATACCACTATTTAATATGGTTGTTATCTATCTTGTATAGATCTTCGAAGTTGCACCATCCCTTTTTATGGTGGATGTTCGAAAGGCCGCTGGTGAAACTCTTGAATATCACAAGGTTAGATTCCTACTGGATTATTCAGATTCATGCACTCAAATATTCTTATTTTGTTCCCCTCCAAAACAAAAAAAAAAACACTATTTTGCAAAAATTACATGCCTGCAGTTCTCTTTTTGCAGTTTTACAAAAATTTCTGTGCCAAGTTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009705 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0031090 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

501

Amino Acids

57.34

Weight (kDa)

9.27

Isoelectric Point (pI)

37.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	13 - 266	1.4e-72	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	15 - 261	3.5e-36	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	112 - 215	1.3e-06	Kinase-like
NAF	PF03822	309 - 368	9.9e-20	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1172
AccI	GTMKAC	2 cut(s)	396, 1239
AciI	CCGC	3 cut(s)	921, 1036, 1325
AclWI	GGATC	4 cut(s)	737, 782, 795, 891
AcoI	YGGCCR	2 cut(s)	132, 244
AcsI	RAATTY	5 cut(s)	445, 718, 973, 1111, 1484
AcuI	CTGAAG	2 cut(s)	308, 431
AfiI	CCNNNNNNNGG	4 cut(s)	488, 489, 490, 1305
AflIII	ACRYGT	1 cut(s)	512
AgsI	TTSAA	8 cut(s)	451, 821, 1062, 1117, 1130, 1206, 1231, 1342
AjnI	CCWGG	2 cut(s)	89, 709
AjuI	GAANNNNNNNTTGG	4 cut(s)	68, 100, 775, 807
AluBI	AGCT	5 cut(s)	358, 575, 1046, 1073, 1186
AluI	AGCT	5 cut(s)	358, 575, 1046, 1073, 1186
Alw26I	GTCTC	6 cut(s)	95, 378, 399, 635, 991, 1097
AlwI	GGATC	4 cut(s)	737, 782, 795, 891
AlwNI	CAGNNNCTG	1 cut(s)	578
Ama87I	CYCGRG	1 cut(s)	1172
AoxI	GGCC	3 cut(s)	132, 244, 1322
ApeKI	GCWGC	4 cut(s)	355, 572, 575, 677
ApoI	RAATTY	5 cut(s)	445, 718, 973, 1111, 1484
AseI	ATTAAT	3 cut(s)	672, 725, 932
Asp700I	GAANNNNTTC	1 cut(s)	423
AspLEI	GCGC	1 cut(s)	814
AspS9I	GGNCC	2 cut(s)	923, 1099
AsuHPI	GGTGA	2 cut(s)	28, 1342
AsuII	TTCGAA	2 cut(s)	1283, 1317
AvaI	CYCGRG	1 cut(s)	1172
AvaII	GGWCC	2 cut(s)	923, 1099
AxyI	CCTNAGG	1 cut(s)	540
BalI	TGGCCA	1 cut(s)	134
BamHI	GGATCC	1 cut(s)	787
BbsI	GAAGAC	1 cut(s)	491
BbvI	GCAGC	4 cut(s)	367, 562, 584, 689
BccI	CCATC	3 cut(s)	560, 767, 1302
BceAI	ACGGC	2 cut(s)	104, 934
BcgI	CGANNNNNNTGC	2 cut(s)	98, 132
BciT130I	CCWGG	2 cut(s)	91, 711
BclI	TGATCA	1 cut(s)	166
BcoDI	GTCTC	6 cut(s)	95, 378, 399, 635, 991, 1097
BfmI	CTRYAG	1 cut(s)	1456
BglII	AGATCT	1 cut(s)	1277
BisI	GCNGC	5 cut(s)	356, 573, 576, 678, 1325
BlsI	GCNGC	5 cut(s)	357, 574, 577, 679, 1326
Bme1390I	CCNGG	2 cut(s)	91, 711
Bme18I	GGWCC	2 cut(s)	923, 1099
BmeT110I	CYCGRG	1 cut(s)	1172
BmgT120I	GGNCC	2 cut(s)	923, 1099
BmiI	GGNNCC	3 cut(s)	67, 520, 789
BmrFI	CCNGG	2 cut(s)	91, 711
BmsI	GCATC	4 cut(s)	217, 355, 614, 1213
BoxI	GACNNNNGTC	1 cut(s)	921
BpiI	GAAGAC	1 cut(s)	491
BpmI	CTGGAG	1 cut(s)	693
Bpu14I	TTCGAA	2 cut(s)	1283, 1317
BpuEI	CTTGAG	3 cut(s)	134, 290, 1079
BsaBI	GATNNNNATC	1 cut(s)	747
BsaI	GGTCTC	3 cut(s)	95, 399, 1097
BsaJI	CCNNGG	4 cut(s)	75, 710, 791, 964
BsaWI	WCCGGW	1 cut(s)	281
Bsc4I	CCNNNNNNNGG	4 cut(s)	488, 489, 490, 1305
Bse118I	RCCGGY	1 cut(s)	104
Bse1I	ACTGG	1 cut(s)	1370
Bse21I	CCTNAGG	1 cut(s)	540
Bse3DI	GCAATG	1 cut(s)	476
Bse8I	GATNNNNATC	1 cut(s)	747
BseBI	CCWGG	2 cut(s)	91, 711
BseDI	CCNNGG	4 cut(s)	75, 710, 791, 964
BseGI	GGATG	6 cut(s)	208, 600, 629, 1228, 1294, 1317
BseJI	GATNNNNATC	1 cut(s)	747
BseLI	CCNNNNNNNGG	4 cut(s)	488, 489, 490, 1305
BseMI	GCAATG	1 cut(s)	476
BseMII	CTCAG	1 cut(s)	531
BseNI	ACTGG	1 cut(s)	1370
BseRI	GAGGAG	1 cut(s)	300
BseXI	GCAGC	4 cut(s)	367, 562, 584, 689
BseYI	CCCAGC	3 cut(s)	483, 547, 1151
BsgI	GTGCAG	2 cut(s)	591, 882
Bsh1285I	CGRYCG	1 cut(s)	57
BshFI	GGCC	3 cut(s)	134, 246, 1324
BsiEI	CGRYCG	1 cut(s)	57
BsiHKCI	CYCGRG	1 cut(s)	1172
BsiSI	CCGG	3 cut(s)	105, 247, 282
BslI	CCNNNNNNNGG	4 cut(s)	488, 489, 490, 1305
BsmAI	GTCTC	6 cut(s)	95, 378, 399, 635, 991, 1097
BsmI	GAATGC	1 cut(s)	943
BsnI	GGCC	3 cut(s)	134, 246, 1324
Bso31I	GGTCTC	3 cut(s)	95, 399, 1097
BsoBI	CYCGRG	1 cut(s)	1172
Bsp119I	TTCGAA	2 cut(s)	1283, 1317
Bsp143I	GATC	6 cut(s)	54, 166, 742, 787, 883, 1277
BspACI	CCGC	3 cut(s)	921, 1036, 1325
BspANI	GGCC	3 cut(s)	134, 246, 1324
BspCNI	CTCAG	1 cut(s)	532
BspHI	TCATGA	1 cut(s)	637
BspLI	GGNNCC	3 cut(s)	67, 520, 789
BspMAI	CTGCAG	1 cut(s)	1460
BspPI	GGATC	4 cut(s)	737, 782, 795, 891
BspT104I	TTCGAA	2 cut(s)	1283, 1317
BspTNI	GGTCTC	3 cut(s)	95, 399, 1097
BsrDI	GCAATG	1 cut(s)	476
BsrFI	RCCGGY	1 cut(s)	104
BsrI	ACTGG	1 cut(s)	1370
BssAI	RCCGGY	1 cut(s)	104
BssECI	CCNNGG	4 cut(s)	75, 710, 791, 964
BssMI	GATC	6 cut(s)	54, 166, 742, 787, 883, 1277
BssNAI	GTATAC	2 cut(s)	397, 1240
BssT1I	CCWWGG	3 cut(s)	75, 791, 964
Bst1107I	GTATAC	2 cut(s)	397, 1240
Bst2UI	CCWGG	2 cut(s)	91, 711
Bst4CI	ACNGT	3 cut(s)	386, 684, 1237
BstBI	TTCGAA	2 cut(s)	1283, 1317
BstC8I	GCNNGC	2 cut(s)	244, 1456
BstDEI	CTNAG	2 cut(s)	470, 540
BstF5I	GGATG	6 cut(s)	208, 600, 629, 1228, 1294, 1317
BstHHI	GCGC	1 cut(s)	814
BstKTI	GATC	6 cut(s)	57, 169, 745, 790, 886, 1280
BstMAI	GTCTC	6 cut(s)	95, 378, 399, 635, 991, 1097
BstMBI	GATC	6 cut(s)	54, 166, 742, 787, 883, 1277
BstMCI	CGRYCG	1 cut(s)	57
BstMWI	GCNNNNNNNGC	3 cut(s)	95, 1148, 1192
BstNI	CCWGG	2 cut(s)	91, 711
BstNSI	RCATGY	2 cut(s)	516, 1454
BstPAI	GACNNNNGTC	1 cut(s)	921
BstSCI	CCNGG	2 cut(s)	89, 709
BstSFI	CTRYAG	1 cut(s)	1456
BstV1I	GCAGC	4 cut(s)	367, 562, 584, 689
BstV2I	GAAGAC	1 cut(s)	491
BstX2I	RGATCY	2 cut(s)	787, 1277
BstYI	RGATCY	2 cut(s)	787, 1277
BstZ17I	GTATAC	2 cut(s)	397, 1240
Bsu36I	CCTNAGG	1 cut(s)	540
BsuRI	GGCC	3 cut(s)	134, 246, 1324
BtsCI	GGATG	6 cut(s)	208, 600, 629, 1228, 1294, 1317
BtsI	GCAGTG	1 cut(s)	375
BtsIMutI	CAGTG	1 cut(s)	375
Cac8I	GCNNGC	2 cut(s)	244, 1456
CaiI	CAGNNNCTG	1 cut(s)	578
CciI	TCATGA	1 cut(s)	637
CfoI	GCGC	1 cut(s)	814
Cfr10I	RCCGGY	1 cut(s)	104
Cfr13I	GGNCC	2 cut(s)	923, 1099
CviAII	CATG	6 cut(s)	230, 513, 638, 1196, 1382, 1451
DdeI	CTNAG	2 cut(s)	470, 540
DpnI	GATC	6 cut(s)	56, 168, 744, 789, 885, 1279
DpnII	GATC	6 cut(s)	54, 166, 742, 787, 883, 1277
EaeI	YGGCCR	2 cut(s)	132, 244
Eco130I	CCWWGG	3 cut(s)	75, 791, 964
Eco31I	GGTCTC	3 cut(s)	95, 399, 1097
Eco32I	GATATC	1 cut(s)	186
Eco47I	GGWCC	2 cut(s)	923, 1099
Eco57I	CTGAAG	2 cut(s)	308, 431
Eco81I	CCTNAGG	1 cut(s)	540
Eco88I	CYCGRG	1 cut(s)	1172
EcoRII	CCWGG	2 cut(s)	89, 709
EcoRV	GATATC	1 cut(s)	186
EcoT14I	CCWWGG	3 cut(s)	75, 791, 964
EcoT22I	ATGCAT	1 cut(s)	943
ErhI	CCWWGG	3 cut(s)	75, 791, 964
FaeI	CATG	6 cut(s)	233, 516, 641, 1199, 1385, 1454
FalI	AAGNNNNNCTT	2 cut(s)	306, 338
FatI	CATG	6 cut(s)	229, 512, 637, 1195, 1381, 1450
FauI	CCCGC	1 cut(s)	1043
FbaI	TGATCA	1 cut(s)	166
FblI	GTMKAC	2 cut(s)	396, 1239
Fnu4HI	GCNGC	5 cut(s)	356, 573, 576, 678, 1325
FokI	GGATG	6 cut(s)	195, 587, 636, 1235, 1281, 1324
Fsp4HI	GCNGC	5 cut(s)	356, 573, 576, 678, 1325
GlaI	GCGC	1 cut(s)	813
GluI	GCNGC	5 cut(s)	356, 573, 576, 678, 1325
GsaI	CCCAGC	3 cut(s)	487, 551, 1155
GsuI	CTGGAG	1 cut(s)	693
HaeIII	GGCC	3 cut(s)	134, 246, 1324
HapII	CCGG	3 cut(s)	105, 247, 282
HhaI	GCGC	1 cut(s)	814
Hin1II	CATG	6 cut(s)	233, 516, 641, 1199, 1385, 1454
Hin6I	GCGC	1 cut(s)	812
HinP1I	GCGC	1 cut(s)	812
HindIII	AAGCTT	1 cut(s)	1184
HinfI	GANTC	6 cut(s)	335, 434, 777, 1082, 1358, 1378
HpaII	CCGG	3 cut(s)	105, 247, 282
HphI	GGTGA	2 cut(s)	28, 1342
Hpy166II	GTNNAC	2 cut(s)	397, 1240
Hpy188I	TCNGA	6 cut(s)	171, 213, 460, 768, 782, 1377
Hpy188III	TCNNGA	6 cut(s)	269, 326, 638, 1117, 1217, 1339
Hpy8I	GTNNAC	2 cut(s)	397, 1240
HpyAV	CCTTC	7 cut(s)	7, 56, 79, 118, 312, 434, 1124
HpyCH4III	ACNGT	3 cut(s)	386, 684, 1237
HpyF10VI	GCNNNNNNNGC	3 cut(s)	95, 1148, 1192
HpyF3I	CTNAG	2 cut(s)	470, 540
Hsp92II	CATG	6 cut(s)	233, 516, 641, 1199, 1385, 1454
HspAI	GCGC	1 cut(s)	812
Ksp22I	TGATCA	1 cut(s)	166
Kzo9I	GATC	6 cut(s)	54, 166, 742, 787, 883, 1277
Lsp1109I	GCAGC	4 cut(s)	367, 562, 584, 689
LweI	GCATC	4 cut(s)	217, 355, 614, 1213
MaeIII	GTNAC	3 cut(s)	16, 277, 380
MalI	GATC	6 cut(s)	56, 168, 744, 789, 885, 1279
MboI	GATC	6 cut(s)	54, 166, 742, 787, 883, 1277
MboII	GAAGA	8 cut(s)	19, 22, 208, 491, 494, 842, 908, 1272
MflI	RGATCY	2 cut(s)	787, 1277
MlsI	TGGCCA	1 cut(s)	134
MluNI	TGGCCA	1 cut(s)	134
Mox20I	TGGCCA	1 cut(s)	134
Mph1103I	ATGCAT	1 cut(s)	943
MroXI	GAANNNNTTC	1 cut(s)	423
MscI	TGGCCA	1 cut(s)	134
MseI	TTAA	8 cut(s)	81, 174, 614, 672, 725, 932, 1182, 1250
Msp20I	TGGCCA	1 cut(s)	134
MspA1I	CMGCKG	2 cut(s)	575, 1327
MspI	CCGG	3 cut(s)	105, 247, 282
MspR9I	CCNGG	2 cut(s)	91, 711
Mva1269I	GAATGC	1 cut(s)	943
MvaI	CCWGG	2 cut(s)	91, 711
MwoI	GCNNNNNNNGC	3 cut(s)	95, 1148, 1192
NdeII	GATC	6 cut(s)	54, 166, 742, 787, 883, 1277
NlaIII	CATG	6 cut(s)	233, 516, 641, 1199, 1385, 1454
NlaIV	GGNNCC	3 cut(s)	67, 520, 789
NmeAIII	GCCGAG	1 cut(s)	124
NmuCI	GTSAC	2 cut(s)	16, 380
NsiI	ATGCAT	1 cut(s)	943
NspI	RCATGY	2 cut(s)	516, 1454
NspV	TTCGAA	2 cut(s)	1283, 1317
PaeR7I	CTCGAG	1 cut(s)	1172
PagI	TCATGA	1 cut(s)	637
PciI	ACATGT	1 cut(s)	512
PcsI	WCGNNNNNNNCGW	1 cut(s)	1021
PctI	GAATGC	1 cut(s)	943
PdmI	GAANNNNTTC	1 cut(s)	423
PfeI	GAWTC	6 cut(s)	335, 434, 777, 1082, 1358, 1378
PkrI	GCNGC	5 cut(s)	357, 574, 577, 679, 1326
Ple19I	CGATCG	1 cut(s)	57
PscI	ACATGT	1 cut(s)	512
PshAI	GACNNNNGTC	1 cut(s)	921
PshBI	ATTAAT	3 cut(s)	672, 725, 932
Psp6I	CCWGG	2 cut(s)	89, 709
PspFI	CCCAGC	3 cut(s)	483, 547, 1151
PspGI	CCWGG	2 cut(s)	89, 709
PspN4I	GGNNCC	3 cut(s)	67, 520, 789
PspPI	GGNCC	2 cut(s)	923, 1099
PspXI	VCTCGAGB	1 cut(s)	1172
PstI	CTGCAG	1 cut(s)	1460
PstNI	CAGNNNCTG	1 cut(s)	578
PsuI	RGATCY	2 cut(s)	787, 1277
PvuI	CGATCG	1 cut(s)	57
PvuII	CAGCTG	1 cut(s)	575
SaqAI	TTAA	8 cut(s)	81, 174, 614, 672, 725, 932, 1182, 1250
SatI	GCNGC	5 cut(s)	356, 573, 576, 678, 1325
Sau3AI	GATC	6 cut(s)	54, 166, 742, 787, 883, 1277
Sau96I	GGNCC	2 cut(s)	923, 1099
ScrFI	CCNGG	2 cut(s)	91, 711
SfaNI	GCATC	4 cut(s)	217, 355, 614, 1213
SfcI	CTRYAG	1 cut(s)	1456
Sfr274I	CTCGAG	1 cut(s)	1172
SfuI	TTCGAA	2 cut(s)	1283, 1317
SinI	GGWCC	2 cut(s)	923, 1099
SlaI	CTCGAG	1 cut(s)	1172
SmlI	CTYRAG	4 cut(s)	149, 269, 1094, 1172
SmoI	CTYRAG	4 cut(s)	149, 269, 1094, 1172
SsiI	CCGC	3 cut(s)	921, 1036, 1325
SspI	AATATT	1 cut(s)	1394
StyD4I	CCNGG	2 cut(s)	89, 709
StyI	CCWWGG	3 cut(s)	75, 791, 964
TaaI	ACNGT	3 cut(s)	386, 684, 1237
TaqI	TCGA	4 cut(s)	990, 1173, 1283, 1317
TaqII	GACCGA	1 cut(s)	485
TauI	GCSGC	1 cut(s)	1327
TfiI	GAWTC	6 cut(s)	335, 434, 777, 1082, 1358, 1378
Tru1I	TTAA	8 cut(s)	81, 174, 614, 672, 725, 932, 1182, 1250
Tru9I	TTAA	8 cut(s)	81, 174, 614, 672, 725, 932, 1182, 1250
TscAI	CASTG	1 cut(s)	375
TseFI	GTSAC	2 cut(s)	16, 380
TseI	GCWGC	4 cut(s)	355, 572, 575, 677
Tsp45I	GTSAC	2 cut(s)	16, 380
TspDTI	ATGAA	9 cut(s)	20, 209, 299, 348, 626, 678, 711, 950, 1370
TspGWI	ACGGA	1 cut(s)	1123
TspRI	CASTG	1 cut(s)	375
VpaK11BI	GGWCC	2 cut(s)	923, 1099
VspI	ATTAAT	3 cut(s)	672, 725, 932
XapI	RAATTY	5 cut(s)	445, 718, 973, 1111, 1484
XceI	RCATGY	2 cut(s)	516, 1454
XhoI	CTCGAG	1 cut(s)	1172
XmiI	GTMKAC	2 cut(s)	396, 1239
XmnI	GAANNNNTTC	1 cut(s)	423
Zsp2I	ATGCAT	1 cut(s)	943

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.