Rh4BG140200

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
N/A
Physical Location & Seq
Reverse (-)
24896686 .. 24899552
2867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG140200.1

Sequence Viewer

Length: 1227 bp
ATGGGAAGTTGCGTTTGTACACCTGCCAAAGCAGGTTTACTACACAAAAAACACAGAGATCAGGTGATCAAGCCTATACCCCAATGTGTTCATGACTCGCCTCAAAAGTCGTCGAACCACCGCGGGGGCTTCTCGGCCCCCATCTCGGTGCTGGACAAGACGGATGGAGAAAACATCCTTGACAAGTACAAGTTGGGCAAGGAGTTGGGGCGAGGGGAGTTTGGAATCACCCACCGATGCGTGGACCGGCAGACCGGCGAGACCTTCGCGTGCAAGGTGATATCAAAGAGCAAGTTGAAGACAGAGATTGACATTGAAGACGTGAGGAGGGAGGCCGAGATCATGAGGCTTTTGCCCAAGCACCCCAACATCGTCACCTTCAAGGAGGCTTATGAGGACAAGGATGCAGTTTATTTTGTCATGGAGCTCTGTGAGGGCGGCGAGCTTTTCGATAGGATCGTCACAAAAGGCCACTACACAGAGCGCGCCGCCGCCCACGTTACCAAGACAATTCTCGAGGTTTGCAAGAGTACTAATTATCCTCTCGCACATATCCAATATGAAGATCATCCCTGGATACGAAATGCAAATCAAGTTCCGAATGTTCCTCTGGCAGAAAATGTCAGATTAAGGATCAAGCAGTTCACATTGATGAATAAATTTAAGAAGAAAGTTCTCAGAGTGGTGGCAGACAACCTGCCAGATGAACAAATAGAAGGAATCAGACAGATATTTGATATGATGGACACCGATAACAATGGAGACTTGACTTTTGAAGAGCTTAGGGATGGTCTTCTCAAGATTGGACACCAAGTCACTGATCCTGATGTGCAAATGTTAATTGATGCAGCGGATGTCGATGGAAGTGGCACACTGAACTGTGAGGAGTTCGTTATTGTGTCTGTTCACTTGAAAAAAATAAGCAGCGACGAACTCCTCGCTCAAGCTTTTAGCTATTTTGACAAGAACAACAGCAACTATATTGAGTTTGATGAGTTAAGAGAAGCATTGCTGGATGATAAACTTGGTCCTACTAATGAGCAGGTTATCCAAGACATCATATTCGATGTGGACTTGGACAAGGATGGGCGAATCGGTTATGAGGAGTTTAAAGCAATGATGAAAACCGGTATGGACTGGAAAATGGCTTCTCGTCAATATTCAAGAGCAATGCTAAATGTACTTAGCCGAAAGTTGTTAAAAGACAAGTCGATCCAAGTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010769 GO:0010857 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043269 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0046777 GO:0047484 GO:0048519 GO:0048583 GO:0048585 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080092 GO:0080134 GO:0097305 GO:0097306 GO:0140096 GO:1901000 GO:1901001 GO:1901016 GO:1901379 GO:1901564 GO:1901700 GO:1901701 GO:1901979 GO:1904062 GO:2000241 GO:2001257
KEGG Pathways
Metabolic & Signaling
ko04626 ko05145 map04626 map05145
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

408

Amino Acids

46.48

Weight (kDa)

5.81

Isoelectric Point (pI)

29.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 63 - 173 3.4e-26 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 64 - 180 8.3e-13 Protein tyrosine and serine/threonine kinase
EF-hand_7 PF13499 239 - 298 1.4e-10 EF-hand domain pair
EF-hand_1 PF00036 241 - 265 6.6e-06 EF hand domain
EF-hand_6 PF13405 241 - 269 5.1e-06 EF-hand domain
EF-hand_8 PF13833 253 - 303 5e-08 EF-hand domain pair
EF-hand_7 PF13499 313 - 375 9.3e-14 EF-hand domain pair
EF-hand_1 PF00036 350 - 376 5e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 4 cut(s) 23, 31, 705, 1033
AccII CGCG 3 cut(s) 123, 269, 486
AciI CCGC 6 cut(s) 121, 123, 438, 489, 492, 851
AclWI GGATC 4 cut(s) 464, 641, 815, 1207
AcsI RAATTY 1 cut(s) 659
AfaI GTAC 4 cut(s) 19, 188, 532, 1182
AfiI CCNNNNNNNGG 3 cut(s) 124, 145, 241
AgeI ACCGGT 1 cut(s) 1127
AgsI TTSAA 6 cut(s) 298, 317, 382, 776, 913, 1164
AhdI GACNNNNNGTC 1 cut(s) 812
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 1 cut(s) 572
AluBI AGCT 5 cut(s) 427, 445, 781, 947, 954
AluI AGCT 5 cut(s) 427, 445, 781, 947, 954
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 2 cut(s) 254, 756
AlwI GGATC 4 cut(s) 464, 641, 815, 1207
Ama87I CYCGRG 1 cut(s) 515
AoxI GGCC 3 cut(s) 135, 333, 469
ApeKI GCWGC 2 cut(s) 848, 924
ApoI RAATTY 1 cut(s) 659
ArsI GACNNNNNNTTYG 4 cut(s) 755, 787, 1046, 1078
AsiGI ACCGGT 1 cut(s) 1127
AspLEI GCGC 2 cut(s) 486, 488
AspS9I GGNCC 3 cut(s) 136, 244, 1028
AsuHPI GGTGA 4 cut(s) 76, 220, 289, 367
AvaI CYCGRG 1 cut(s) 515
AvaII GGWCC 2 cut(s) 244, 1028
BanII GRGCYC 1 cut(s) 429
BbsI GAAGAC 3 cut(s) 305, 324, 785
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 860, 936
BccI CCATC 6 cut(s) 149, 158, 736, 782, 854, 1079
BciT130I CCWGG 1 cut(s) 574
BciVI GTATCC 1 cut(s) 570
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 254, 756
BfuAI ACCTGC 4 cut(s) 23, 31, 705, 1033
BfuI GTATCC 1 cut(s) 570
BisI GCNGC 5 cut(s) 439, 489, 492, 849, 925
BlsI GCNGC 5 cut(s) 440, 490, 493, 850, 926
BmcAI AGTACT 1 cut(s) 532
Bme1390I CCNGG 1 cut(s) 574
Bme18I GGWCC 2 cut(s) 244, 1028
BmeRI GACNNNNNGTC 1 cut(s) 812
BmeT110I CYCGRG 1 cut(s) 515
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 3 cut(s) 136, 244, 1028
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 1 cut(s) 574
BmsI GCATC 3 cut(s) 227, 394, 835
BpiI GAAGAC 3 cut(s) 305, 324, 785
Bpu10I CCTNAGC 1 cut(s) 782
BpuEI CTTGAG 2 cut(s) 782, 927
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 2 cut(s) 121, 572
BsaWI WCCGGW 1 cut(s) 1127
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 145, 241
Bse118I RCCGGY 3 cut(s) 246, 254, 1127
Bse1I ACTGG 1 cut(s) 1142
Bse3DI GCAATG 3 cut(s) 1007, 1122, 1176
BseBI CCWGG 1 cut(s) 574
BseDI CCNNGG 2 cut(s) 121, 572
BseGI GGATG 8 cut(s) 169, 174, 409, 568, 793, 859, 1021, 1090
BseLI CCNNNNNNNGG 3 cut(s) 124, 145, 241
BseMI GCAATG 3 cut(s) 1007, 1122, 1176
BseMII CTCAG 1 cut(s) 691
BseNI ACTGG 1 cut(s) 1142
BsePI GCGCGC 1 cut(s) 484
BseRI GAGGAG 4 cut(s) 340, 899, 926, 1118
BseXI GCAGC 2 cut(s) 860, 936
Bsh1236I CGCG 3 cut(s) 123, 269, 486
BshFI GGCC 3 cut(s) 137, 335, 471
BshTI ACCGGT 1 cut(s) 1127
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 515
BsiSI CCGG 3 cut(s) 247, 255, 1128
BslI CCNNNNNNNGG 3 cut(s) 124, 145, 241
BsmAI GTCTC 2 cut(s) 254, 756
BsnI GGCC 3 cut(s) 137, 335, 471
Bso31I GGTCTC 1 cut(s) 254
BsoBI CYCGRG 1 cut(s) 515
Bsp1286I GDGCHC 1 cut(s) 429
Bsp1407I TGTACA 1 cut(s) 17
Bsp143I GATC 8 cut(s) 58, 66, 339, 456, 565, 633, 820, 1212
BspACI CCGC 6 cut(s) 121, 123, 438, 489, 492, 851
BspANI GGCC 3 cut(s) 137, 335, 471
BspCNI CTCAG 1 cut(s) 690
BspFNI CGCG 3 cut(s) 123, 269, 486
BspHI TCATGA 2 cut(s) 91, 342
BspLI GGNNCC 1 cut(s) 138
BspMI ACCTGC 4 cut(s) 23, 31, 705, 1033
BspPI GGATC 4 cut(s) 464, 641, 815, 1207
BspQI GCTCTTC 1 cut(s) 771
BspTNI GGTCTC 1 cut(s) 254
BsrDI GCAATG 3 cut(s) 1007, 1122, 1176
BsrFI RCCGGY 3 cut(s) 246, 254, 1127
BsrGI TGTACA 1 cut(s) 17
BsrI ACTGG 1 cut(s) 1142
BssAI RCCGGY 3 cut(s) 246, 254, 1127
BssECI CCNNGG 2 cut(s) 121, 572
BssHII GCGCGC 1 cut(s) 484
BssMI GATC 8 cut(s) 58, 66, 339, 456, 565, 633, 820, 1212
Bst2UI CCWGG 1 cut(s) 574
Bst4CI ACNGT 1 cut(s) 881
Bst6I CTCTTC 1 cut(s) 771
BstAUI TGTACA 1 cut(s) 17
BstC8I GCNNGC 3 cut(s) 271, 443, 486
BstDEI CTNAG 3 cut(s) 677, 782, 1184
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 8 cut(s) 169, 174, 409, 568, 793, 859, 1021, 1090
BstFNI CGCG 3 cut(s) 123, 269, 486
BstHHI GCGC 2 cut(s) 486, 488
BstKTI GATC 8 cut(s) 61, 69, 342, 459, 568, 636, 823, 1215
BstMAI GTCTC 2 cut(s) 254, 756
BstMBI GATC 8 cut(s) 58, 66, 339, 456, 565, 633, 820, 1212
BstNI CCWGG 1 cut(s) 574
BstSCI CCNGG 1 cut(s) 572
BstUI CGCG 3 cut(s) 123, 269, 486
BstV1I GCAGC 2 cut(s) 860, 936
BstV2I GAAGAC 3 cut(s) 305, 324, 785
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 3 cut(s) 137, 335, 471
BtgI CCRYGG 1 cut(s) 121
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 8 cut(s) 169, 174, 409, 568, 793, 859, 1021, 1090
BtsIMutI CAGTG 2 cut(s) 816, 872
BveI ACCTGC 4 cut(s) 23, 31, 705, 1033
Cac8I GCNNGC 3 cut(s) 271, 443, 486
CciI TCATGA 2 cut(s) 91, 342
CfoI GCGC 2 cut(s) 486, 488
Cfr10I RCCGGY 3 cut(s) 246, 254, 1127
Cfr13I GGNCC 3 cut(s) 136, 244, 1028
Cfr42I CCGCGG 1 cut(s) 124
Csp6I GTAC 4 cut(s) 18, 187, 531, 1181
CspAI ACCGGT 1 cut(s) 1127
CviAII CATG 3 cut(s) 92, 343, 421
CviQI GTAC 4 cut(s) 18, 187, 531, 1181
DdeI CTNAG 3 cut(s) 677, 782, 1184
DpnI GATC 8 cut(s) 60, 68, 341, 458, 567, 635, 822, 1214
DpnII GATC 8 cut(s) 58, 66, 339, 456, 565, 633, 820, 1212
DraI TTTAAA 1 cut(s) 1111
DriI GACNNNNNGTC 1 cut(s) 812
Eam1104I CTCTTC 1 cut(s) 771
Eam1105I GACNNNNNGTC 1 cut(s) 812
EarI CTCTTC 1 cut(s) 771
Ecl136II GAGCTC 1 cut(s) 427
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 1 cut(s) 254
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 2 cut(s) 244, 1028
Eco53kI GAGCTC 1 cut(s) 427
Eco88I CYCGRG 1 cut(s) 515
EcoICRI GAGCTC 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 572
EcoRV GATATC 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 429
FaeI CATG 3 cut(s) 95, 346, 424
FatI CATG 3 cut(s) 91, 342, 420
FauI CCCGC 1 cut(s) 116
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 5 cut(s) 439, 489, 492, 849, 925
FokI GGATG 8 cut(s) 161, 176, 416, 555, 800, 866, 1028, 1097
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 5 cut(s) 439, 489, 492, 849, 925
GlaI GCGC 2 cut(s) 485, 487
GluI GCNGC 5 cut(s) 439, 489, 492, 849, 925
HaeIII GGCC 3 cut(s) 137, 335, 471
HapII CCGG 3 cut(s) 247, 255, 1128
HhaI GCGC 2 cut(s) 486, 488
Hin1II CATG 3 cut(s) 95, 346, 424
Hin6I GCGC 2 cut(s) 484, 486
HinP1I GCGC 2 cut(s) 484, 486
HindIII AAGCTT 1 cut(s) 945
HinfI GANTC 4 cut(s) 95, 225, 720, 1092
HpaII CCGG 3 cut(s) 247, 255, 1128
HphI GGTGA 4 cut(s) 76, 220, 289, 367
Hpy166II GTNNAC 7 cut(s) 20, 38, 244, 645, 907, 1072, 1222
Hpy188I TCNGA 4 cut(s) 600, 626, 680, 725
Hpy188III TCNNGA 6 cut(s) 92, 343, 515, 799, 824, 1164
Hpy8I GTNNAC 7 cut(s) 20, 38, 244, 645, 907, 1072, 1222
Hpy99I CGWCG 2 cut(s) 115, 932
HpyAV CCTTC 3 cut(s) 274, 388, 710
HpyCH4III ACNGT 1 cut(s) 881
HpyCH4IV ACGT 2 cut(s) 321, 498
HpyCH4V TGCA 6 cut(s) 273, 407, 525, 587, 832, 848
HpyF3I CTNAG 3 cut(s) 677, 782, 1184
HpySE526I ACGT 2 cut(s) 321, 498
Hsp92II CATG 3 cut(s) 95, 346, 424
HspAI GCGC 2 cut(s) 484, 486
Ksp22I TGATCA 1 cut(s) 66
KspI CCGCGG 1 cut(s) 124
Kzo9I GATC 8 cut(s) 58, 66, 339, 456, 565, 633, 820, 1212
LguI GCTCTTC 1 cut(s) 771
LmnI GCTCC 1 cut(s) 424
Lsp1109I GCAGC 2 cut(s) 860, 936
LweI GCATC 3 cut(s) 227, 394, 835
MaeII ACGT 2 cut(s) 321, 498
MaeIII GTNAC 4 cut(s) 373, 460, 499, 814
MalI GATC 8 cut(s) 60, 68, 341, 458, 567, 635, 822, 1214
MboI GATC 8 cut(s) 58, 66, 339, 456, 565, 633, 820, 1212
MboII GAAGA 6 cut(s) 310, 329, 575, 679, 785, 788
MhlI GDGCHC 1 cut(s) 429
MluCI AATT 4 cut(s) 510, 535, 659, 840
MlyI GAGTC 1 cut(s) 89
MseI TTAA 6 cut(s) 629, 663, 839, 998, 1110, 1199
MslI CAYNNNNRTG 2 cut(s) 146, 650
MspA1I CMGCKG 2 cut(s) 123, 851
MspI CCGG 3 cut(s) 247, 255, 1128
MspR9I CCNGG 1 cut(s) 574
MvaI CCWGG 1 cut(s) 574
MvnI CGCG 3 cut(s) 123, 269, 486
NdeII GATC 8 cut(s) 58, 66, 339, 456, 565, 633, 820, 1212
NlaIII CATG 3 cut(s) 95, 346, 424
NlaIV GGNNCC 1 cut(s) 138
NmeAIII GCCGAG 2 cut(s) 113, 361
NmuCI GTSAC 3 cut(s) 373, 460, 814
PaeR7I CTCGAG 1 cut(s) 515
PagI TCATGA 2 cut(s) 91, 342
PaqCI CACCTGC 1 cut(s) 31
PauI GCGCGC 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 771
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PfeI GAWTC 3 cut(s) 225, 720, 1092
PinAI ACCGGT 1 cut(s) 1127
PkrI GCNGC 5 cut(s) 440, 490, 493, 850, 926
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 572
PspGI CCWGG 1 cut(s) 572
PspN4I GGNNCC 1 cut(s) 138
PspPI GGNCC 3 cut(s) 136, 244, 1028
PteI GCGCGC 1 cut(s) 484
RsaI GTAC 4 cut(s) 19, 188, 532, 1182
RsaNI GTAC 4 cut(s) 18, 187, 531, 1181
RseI CAYNNNNRTG 2 cut(s) 146, 650
SacI GAGCTC 1 cut(s) 429
SacII CCGCGG 1 cut(s) 124
SapI GCTCTTC 1 cut(s) 771
SaqAI TTAA 6 cut(s) 629, 663, 839, 998, 1110, 1199
SatI GCNGC 5 cut(s) 439, 489, 492, 849, 925
Sau3AI GATC 8 cut(s) 58, 66, 339, 456, 565, 633, 820, 1212
Sau96I GGNCC 3 cut(s) 136, 244, 1028
ScaI AGTACT 1 cut(s) 532
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 574
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 3 cut(s) 227, 394, 835
Sfr274I CTCGAG 1 cut(s) 515
Sfr303I CCGCGG 1 cut(s) 124
SgrBI CCGCGG 1 cut(s) 124
SinI GGWCC 2 cut(s) 244, 1028
SlaI CTCGAG 1 cut(s) 515
SmiMI CAYNNNNRTG 2 cut(s) 146, 650
SmlI CTYRAG 3 cut(s) 515, 797, 942
SmoI CTYRAG 3 cut(s) 515, 797, 942
Sse9I AATT 4 cut(s) 510, 535, 659, 840
SsiI CCGC 6 cut(s) 121, 123, 438, 489, 492, 851
SspI AATATT 1 cut(s) 1160
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 1 cut(s) 572
TaaI ACNGT 1 cut(s) 881
TaiI ACGT 2 cut(s) 324, 501
TaqI TCGA 6 cut(s) 113, 450, 516, 858, 1065, 1211
TasI AATT 4 cut(s) 510, 535, 659, 840
TatI WGTACW 4 cut(s) 17, 186, 530, 1180
TauI GCSGC 3 cut(s) 441, 491, 494
TfiI GAWTC 3 cut(s) 225, 720, 1092
Tru1I TTAA 6 cut(s) 629, 663, 839, 998, 1110, 1199
Tru9I TTAA 6 cut(s) 629, 663, 839, 998, 1110, 1199
TscAI CASTG 2 cut(s) 823, 879
TseFI GTSAC 3 cut(s) 373, 460, 814
TseI GCWGC 2 cut(s) 848, 924
Tsp45I GTSAC 3 cut(s) 373, 460, 814
TspDTI ATGAA 5 cut(s) 80, 576, 668, 720, 1136
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 2 cut(s) 823, 879
VpaK11BI GGWCC 2 cut(s) 244, 1028
XapI RAATTY 1 cut(s) 659
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XhoI CTCGAG 1 cut(s) 515
ZrmI AGTACT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.