Rh4BG391600

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
N/A
Physical Location & Seq
Reverse (-)
54416650 .. 54419403
2754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG391600.1

Sequence Viewer

Length: 867 bp
ATGGGTTTGGAAGCTGAGAATGGGAAAGTGAAAGTGGAGTCTTGGGATATAAGCAAATCCAAGGGGAGGAGGAAGAAGAAGATGGTAGTGGATGATGATGATGATGATGTGGAGGAAGAGGAGACTGGGTGCTGGCTAAGGCTGAGGTTTTTTGGGAGCTGCATTTCTTCAAGATCCAAAGTTGATAGCTCAATTAGTGGCACCAGTACTCACTGTGAAATCAAATCTACAAATGATACAAGCAGAGACCAACCGACGGCTACTGCAGTGTCTAACTCAACCACTAGTAACACAGAAAGTAATGCATCCACTTCAAAATTTGAAGAGGAACTTAAAGTTGCTTCAAGGTTGCGAAAATTTGCTTTTAACGATCTTAAATTGGCAACAAGAAATTTCAGACCTGAAAGTCTTCTTGGTGAAGGTGGTTTTGGTTGTGTGTTCAAGGGGTGGATAGAAGAAAATGGAACTGCTCCAGTCAAACCTGGCACAGGACTTACTGTTGCGGTTAAAACTCTTAATCATGATGGGCTTCAGGGTCATAAAGAATGGCTGGCTGAAGTAAATTTCCTTGGTGACCTTGTCCATCCAAACTTGGTAAAACTTATTGGATACTGCATTGAAGATGATCAAAGGCTGCTAGTGTATGAGTTCATGCCTCGAGGGAGCTTGGAGAACCACCTATTTAGGAGGTCCTTGCCTCTTCCATGGTCTATTAGAATGAAAATTGCATTGGGTGCGGCAAAAGGTCTTACTTTTCTTCATGAGGAGGCAGAAAGACCTGTGATTTATCGTGACTTTAAAACCTCCAACATCTTATTGGATGCAGACATCTTACATCGAGGAGTGATGTCTATAGTTTTGGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

288

Amino Acids

32.12

Weight (kDa)

6.21

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 135 - 275 9e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 136 - 276 1.6e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 405
AbsI CCTCGAGG 1 cut(s) 657
AccB1I GGYRCC 1 cut(s) 200
AciI CCGC 2 cut(s) 503, 737
AclWI GGATC 1 cut(s) 168
AcsI RAATTY 4 cut(s) 317, 356, 391, 562
AcuI CTGAAG 2 cut(s) 515, 576
AfaI GTAC 1 cut(s) 208
AfiI CCNNNNNNNGG 3 cut(s) 66, 256, 488
AgsI TTSAA 6 cut(s) 171, 315, 323, 345, 442, 620
AhlI ACTAGT 1 cut(s) 284
AjnI CCWGG 1 cut(s) 481
AjuI GAANNNNNNNTTGG 6 cut(s) 396, 411, 428, 443, 713, 745
AluBI AGCT 4 cut(s) 14, 159, 189, 666
AluI AGCT 4 cut(s) 14, 159, 189, 666
Alw26I GTCTC 2 cut(s) 116, 240
AlwI GGATC 1 cut(s) 168
Ama87I CYCGRG 1 cut(s) 657
ApeKI GCWGC 2 cut(s) 159, 634
ApoI RAATTY 4 cut(s) 317, 356, 391, 562
Asp700I GAANNNNTTC 1 cut(s) 408
AspS9I GGNCC 1 cut(s) 690
AsuHPI GGTGA 2 cut(s) 428, 584
AvaI CYCGRG 1 cut(s) 657
AvaII GGWCC 1 cut(s) 690
BanI GGYRCC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 401
BbvCI CCTCAGC 1 cut(s) 143
BbvI GCAGC 2 cut(s) 146, 621
BccI CCATC 3 cut(s) 76, 518, 591
BceAI ACGGC 1 cut(s) 273
BciT130I CCWGG 1 cut(s) 483
BciVI GTATCC 1 cut(s) 602
BclI TGATCA 1 cut(s) 625
BcoDI GTCTC 2 cut(s) 116, 240
BcuI ACTAGT 1 cut(s) 284
BfaI CTAG 2 cut(s) 285, 638
BfmI CTRYAG 2 cut(s) 264, 852
BfuI GTATCC 1 cut(s) 602
BisI GCNGC 3 cut(s) 160, 635, 738
BlsI GCNGC 3 cut(s) 161, 636, 739
BmcAI AGTACT 1 cut(s) 208
Bme1390I CCNGG 1 cut(s) 483
Bme18I GGWCC 1 cut(s) 690
BmeT110I CYCGRG 1 cut(s) 657
BmgT120I GGNCC 1 cut(s) 690
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 1 cut(s) 483
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 2 cut(s) 314, 811
BmuI ACTGGG 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 401
BpmI CTGGAG 1 cut(s) 456
Bpu10I CCTNAGC 2 cut(s) 137, 143
BsaI GGTCTC 1 cut(s) 240
BsaJI CCNNGG 3 cut(s) 60, 568, 704
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 256, 488
Bse1I ACTGG 3 cut(s) 130, 204, 473
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 3 cut(s) 60, 568, 704
BseGI GGATG 4 cut(s) 97, 305, 583, 826
BseLI CCNNNNNNNGG 3 cut(s) 66, 256, 488
BseMII CTCAG 2 cut(s) 6, 134
BseNI ACTGG 3 cut(s) 130, 204, 473
BseRI GAGGAG 4 cut(s) 82, 134, 779, 855
BseXI GCAGC 2 cut(s) 146, 621
BshNI GGYRCC 1 cut(s) 200
BsiHKCI CYCGRG 1 cut(s) 657
BslI CCNNNNNNNGG 3 cut(s) 66, 256, 488
BsmAI GTCTC 2 cut(s) 116, 240
Bso31I GGTCTC 1 cut(s) 240
BsoBI CYCGRG 1 cut(s) 657
Bsp143I GATC 3 cut(s) 173, 370, 625
Bsp19I CCATGG 1 cut(s) 704
BspACI CCGC 2 cut(s) 503, 737
BspCNI CTCAG 2 cut(s) 7, 135
BspHI TCATGA 2 cut(s) 520, 760
BspLI GGNNCC 1 cut(s) 202
BspMAI CTGCAG 1 cut(s) 268
BspPI GGATC 1 cut(s) 168
BspT107I GGYRCC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 240
BsrI ACTGG 3 cut(s) 130, 204, 473
BssECI CCNNGG 3 cut(s) 60, 568, 704
BssMI GATC 3 cut(s) 173, 370, 625
BssT1I CCWWGG 3 cut(s) 60, 568, 704
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 2 cut(s) 215, 499
Bst6I CTCTTC 3 cut(s) 111, 318, 705
BstAPI GCANNNNNTGC 1 cut(s) 734
BstC8I GCNNGC 2 cut(s) 134, 552
BstDEI CTNAG 3 cut(s) 15, 137, 143
BstDSI CCRYGG 1 cut(s) 704
BstEII GGTNACC 1 cut(s) 572
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 4 cut(s) 97, 305, 583, 826
BstKTI GATC 3 cut(s) 176, 373, 628
BstMAI GTCTC 2 cut(s) 116, 240
BstMBI GATC 3 cut(s) 173, 370, 625
BstMWI GCNNNNNNNGC 1 cut(s) 734
BstNI CCWGG 1 cut(s) 483
BstPI GGTNACC 1 cut(s) 572
BstSCI CCNGG 1 cut(s) 481
BstSFI CTRYAG 2 cut(s) 264, 852
BstV1I GCAGC 2 cut(s) 146, 621
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
BsuI GTATCC 1 cut(s) 602
BtgI CCRYGG 1 cut(s) 704
BtsCI GGATG 4 cut(s) 97, 305, 583, 826
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 2 cut(s) 211, 273
Cac8I GCNNGC 2 cut(s) 134, 552
CciI TCATGA 2 cut(s) 520, 760
Cfr13I GGNCC 1 cut(s) 690
Csp6I GTAC 1 cut(s) 207
CviAII CATG 4 cut(s) 521, 652, 705, 761
CviQI GTAC 1 cut(s) 207
DdeI CTNAG 3 cut(s) 15, 137, 143
DpnI GATC 3 cut(s) 175, 372, 627
DpnII GATC 3 cut(s) 173, 370, 625
DraI TTTAAA 1 cut(s) 799
DrdI GACNNNNNNGTC 1 cut(s) 405
DseDI GACNNNNNNGTC 1 cut(s) 405
Eam1104I CTCTTC 3 cut(s) 111, 318, 705
EarI CTCTTC 3 cut(s) 111, 318, 705
Eco130I CCWWGG 3 cut(s) 60, 568, 704
Eco31I GGTCTC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 690
Eco57I CTGAAG 2 cut(s) 515, 576
Eco88I CYCGRG 1 cut(s) 657
Eco91I GGTNACC 1 cut(s) 572
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoO109I RGGNCCY 1 cut(s) 690
EcoO65I GGTNACC 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 3 cut(s) 60, 568, 704
EcoT22I ATGCAT 1 cut(s) 307
ErhI CCWWGG 3 cut(s) 60, 568, 704
FaeI CATG 4 cut(s) 524, 655, 708, 764
FaiI YATR 8 cut(s) 50, 522, 540, 645, 653, 706, 762, 854
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FatI CATG 4 cut(s) 520, 651, 704, 760
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 3 cut(s) 160, 635, 738
FokI GGATG 4 cut(s) 104, 292, 570, 833
Fsp4HI GCNGC 3 cut(s) 160, 635, 738
FspBI CTAG 2 cut(s) 285, 638
GluI GCNGC 3 cut(s) 160, 635, 738
GsuI CTGGAG 1 cut(s) 456
Hin1II CATG 4 cut(s) 524, 655, 708, 764
HinfI GANTC 1 cut(s) 38
HphI GGTGA 2 cut(s) 428, 584
Hpy188I TCNGA 1 cut(s) 398
Hpy188III TCNNGA 4 cut(s) 171, 521, 761, 791
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 2 cut(s) 215, 499
HpyCH4V TGCA 6 cut(s) 162, 266, 305, 615, 728, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 734
HpyF3I CTNAG 3 cut(s) 15, 137, 143
Hsp92II CATG 4 cut(s) 524, 655, 708, 764
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 3 cut(s) 173, 370, 625
LmnI GCTCC 3 cut(s) 156, 475, 663
Lsp1109I GCAGC 2 cut(s) 146, 621
LweI GCATC 2 cut(s) 314, 811
MaeI CTAG 2 cut(s) 285, 638
MaeIII GTNAC 3 cut(s) 287, 572, 791
MalI GATC 3 cut(s) 175, 372, 627
MboI GATC 3 cut(s) 173, 370, 625
MflI RGATCY 1 cut(s) 173
MluCI AATT 7 cut(s) 192, 317, 356, 377, 391, 562, 723
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 831
Mph1103I ATGCAT 1 cut(s) 307
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 6 cut(s) 333, 366, 375, 507, 516, 798
MspR9I CCNGG 1 cut(s) 483
MvaI CCWGG 1 cut(s) 483
MwoI GCNNNNNNNGC 1 cut(s) 734
NcoI CCATGG 1 cut(s) 704
NdeII GATC 3 cut(s) 173, 370, 625
NlaIII CATG 4 cut(s) 524, 655, 708, 764
NlaIV GGNNCC 1 cut(s) 202
NmuCI GTSAC 2 cut(s) 572, 791
NsiI ATGCAT 1 cut(s) 307
PaeR7I CTCGAG 1 cut(s) 657
PagI TCATGA 2 cut(s) 520, 760
PdmI GAANNNNTTC 1 cut(s) 408
PflFI GACNNNGTC 1 cut(s) 578
PkrI GCNGC 3 cut(s) 161, 636, 739
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 690
Psp5II RGGWCCY 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 481
PspEI GGTNACC 1 cut(s) 572
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 1 cut(s) 202
PspPI GGNCC 1 cut(s) 690
PspPPI RGGWCCY 1 cut(s) 690
PspXI VCTCGAGB 1 cut(s) 657
PstI CTGCAG 1 cut(s) 268
PsuI RGATCY 1 cut(s) 173
PsyI GACNNNGTC 1 cut(s) 578
RsaI GTAC 1 cut(s) 208
RsaNI GTAC 1 cut(s) 207
SaqAI TTAA 6 cut(s) 333, 366, 375, 507, 516, 798
SatI GCNGC 3 cut(s) 160, 635, 738
Sau3AI GATC 3 cut(s) 173, 370, 625
Sau96I GGNCC 1 cut(s) 690
ScaI AGTACT 1 cut(s) 208
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 1 cut(s) 483
SfaNI GCATC 2 cut(s) 314, 811
SfcI CTRYAG 2 cut(s) 264, 852
Sfr274I CTCGAG 1 cut(s) 657
SinI GGWCC 1 cut(s) 690
SlaI CTCGAG 1 cut(s) 657
SmlI CTYRAG 1 cut(s) 657
SmoI CTYRAG 1 cut(s) 657
SpeI ACTAGT 1 cut(s) 284
Sse9I AATT 7 cut(s) 192, 317, 356, 377, 391, 562, 723
SsiI CCGC 2 cut(s) 503, 737
SspMI CTAG 2 cut(s) 285, 638
StyD4I CCNGG 1 cut(s) 481
StyI CCWWGG 3 cut(s) 60, 568, 704
TaaI ACNGT 2 cut(s) 215, 499
TaqI TCGA 2 cut(s) 658, 838
TasI AATT 7 cut(s) 192, 317, 356, 377, 391, 562, 723
TatI WGTACW 1 cut(s) 206
TauI GCSGC 1 cut(s) 740
Tru1I TTAA 6 cut(s) 333, 366, 375, 507, 516, 798
Tru9I TTAA 6 cut(s) 333, 366, 375, 507, 516, 798
TscAI CASTG 2 cut(s) 218, 273
TseFI GTSAC 2 cut(s) 572, 791
TseI GCWGC 2 cut(s) 159, 634
Tsp45I GTSAC 2 cut(s) 572, 791
TspDTI ATGAA 3 cut(s) 640, 734, 749
TspRI CASTG 2 cut(s) 218, 273
Tth111I GACNNNGTC 1 cut(s) 578
VpaK11BI GGWCC 1 cut(s) 690
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 4 cut(s) 317, 356, 391, 562
XcmI CCANNNNNNNNNTGG 1 cut(s) 814
XhoI CTCGAG 1 cut(s) 657
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 2 cut(s) 285, 638
ZrmI AGTACT 1 cut(s) 208
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.