Rh4CG236700

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
N/A
Physical Location & Seq
Forward (+)
48091992 .. 48096008
4017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG236700.1

Sequence Viewer

Length: 525 bp
ATGCCACCTGGGCTGGGAAAGTTGGTATCTCTTCAGACATTACCGTTATTCATCTTGGGCAGGAAAGCTCTCTTTTCCGGTGTGAGTGGTGGAATAGATGAGTTGAGTGGCTTGAACTGTCTTAAAGGAGTGCTTCATGTTGCCCATCTTGAACATGTTGACAATGTCACAATAGATTCAAGGAGTTCAAATTTGAGCAGCAAGCAATATCTTGAATCCTTAAAACTAATTTGGTCCAGAGATGACGACAGTGAAGTGCGGGGTTGCAGTTTGGCAGAGCAGGGACGCGGCTCAAGTGTGACATTAATGGGCAATGATGCAGTTTTTACGCCTATCACGATGGTTTTCTTCTCTTTCCAACATTGGGACTTCCAGTCGGCGGGGCAGCGACGCCCTACAGAGGTGGAGTGGCGGATCCGAGTGGTTGGATGCTGGAGGATGTGGCGGCGCGGAGTAATGGGTGGCGGCAAGTTGCAGCGTGTTTGGGTGCCCAGAATAGATTATGCTGGGCTTGGATCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

174

Amino Acids

19.33

Weight (kDa)

9.21

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_R13L1-DRL21 PF25019 32 - 84 1.4e-08 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 487
AccII CGCG 2 cut(s) 288, 450
AciI CCGC 7 cut(s) 259, 288, 380, 412, 445, 450, 465
AclWI GGATC 3 cut(s) 409, 422, 523
AcsI RAATTY 1 cut(s) 190
AcuI CTGAAG 1 cut(s) 17
AcyI GRCGYC 1 cut(s) 391
AfiI CCNNNNNNNGG 4 cut(s) 14, 364, 379, 400
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 5 cut(s) 115, 152, 180, 189, 215
AhdI GACNNNNNGTC 1 cut(s) 373
AjnI CCWGG 1 cut(s) 7
AluBI AGCT 1 cut(s) 68
AluI AGCT 1 cut(s) 68
AlwI GGATC 3 cut(s) 409, 422, 523
ApeKI GCWGC 3 cut(s) 198, 385, 475
ApoI RAATTY 1 cut(s) 190
AseI ATTAAT 1 cut(s) 305
AspLEI GCGC 1 cut(s) 450
AspS9I GGNCC 1 cut(s) 234
AvaII GGWCC 1 cut(s) 234
BaeGI GKGCMC 1 cut(s) 492
BamHI GGATCC 1 cut(s) 414
BanI GGYRCC 1 cut(s) 487
BbvI GCAGC 3 cut(s) 210, 397, 487
BccI CCATC 2 cut(s) 153, 334
BciT130I CCWGG 1 cut(s) 9
BfmI CTRYAG 1 cut(s) 396
BglI GCCNNNNNGGC 1 cut(s) 10
BisI GCNGC 6 cut(s) 199, 289, 386, 446, 466, 476
BlsI GCNGC 6 cut(s) 200, 290, 387, 447, 467, 477
Bme1390I CCNGG 1 cut(s) 9
Bme18I GGWCC 1 cut(s) 234
BmeRI GACNNNNNGTC 1 cut(s) 373
BmgT120I GGNCC 1 cut(s) 234
BmiI GGNNCC 2 cut(s) 416, 489
BmrFI CCNGG 1 cut(s) 9
BmsI GCATC 2 cut(s) 307, 419
BpmI CTGGAG 1 cut(s) 454
BpuEI CTTGAG 1 cut(s) 277
BsaHI GRCGYC 1 cut(s) 391
BsaJI CCNNGG 1 cut(s) 8
BsaWI WCCGGW 1 cut(s) 77
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 364, 379, 400
Bse1I ACTGG 1 cut(s) 373
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 1 cut(s) 9
BseDI CCNNGG 1 cut(s) 8
BseGI GGATG 2 cut(s) 434, 444
BseLI CCNNNNNNNGG 4 cut(s) 14, 364, 379, 400
BseMI GCAATG 1 cut(s) 319
BseNI ACTGG 1 cut(s) 373
BseSI GKGCMC 1 cut(s) 492
BseXI GCAGC 3 cut(s) 210, 397, 487
BseYI CCCAGC 2 cut(s) 13, 506
Bsh1236I CGCG 2 cut(s) 288, 450
BshNI GGYRCC 1 cut(s) 487
BsiSI CCGG 1 cut(s) 78
BslFI GGGAC 2 cut(s) 297, 380
BslI CCNNNNNNNGG 4 cut(s) 14, 364, 379, 400
BsmFI GGGAC 2 cut(s) 297, 380
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 2 cut(s) 414, 515
BspACI CCGC 7 cut(s) 259, 288, 380, 412, 445, 450, 465
BspFNI CGCG 2 cut(s) 288, 450
BspLI GGNNCC 2 cut(s) 416, 489
BspPI GGATC 3 cut(s) 409, 422, 523
BspT107I GGYRCC 1 cut(s) 487
BsrDI GCAATG 1 cut(s) 319
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 8
BssMI GATC 2 cut(s) 414, 515
BssNI GRCGYC 1 cut(s) 391
Bst2UI CCWGG 1 cut(s) 9
Bst4CI ACNGT 4 cut(s) 45, 119, 251, 522
Bst6I CTCTTC 1 cut(s) 36
BstACI GRCGYC 1 cut(s) 391
BstC8I GCNNGC 1 cut(s) 203
BstF5I GGATG 2 cut(s) 434, 444
BstFNI CGCG 2 cut(s) 288, 450
BstHHI GCGC 1 cut(s) 450
BstKTI GATC 2 cut(s) 417, 518
BstMBI GATC 2 cut(s) 414, 515
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 9
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 7
BstSFI CTRYAG 1 cut(s) 396
BstSLI GKGCMC 1 cut(s) 492
BstUI CGCG 2 cut(s) 288, 450
BstV1I GCAGC 3 cut(s) 210, 397, 487
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BtsCI GGATG 2 cut(s) 434, 444
BtsIMutI CAGTG 2 cut(s) 256, 518
Cac8I GCNNGC 1 cut(s) 203
CfoI GCGC 1 cut(s) 450
Cfr13I GGNCC 1 cut(s) 234
CseI GACGC 2 cut(s) 294, 399
CviAII CATG 2 cut(s) 137, 155
CviJI RGCY 5 cut(s) 13, 68, 111, 291, 511
CviKI_1 RGCY 5 cut(s) 13, 68, 111, 291, 511
DpnI GATC 2 cut(s) 416, 517
DpnII GATC 2 cut(s) 414, 515
DriI GACNNNNNGTC 1 cut(s) 373
Eam1104I CTCTTC 1 cut(s) 36
Eam1105I GACNNNNNGTC 1 cut(s) 373
EarI CTCTTC 1 cut(s) 36
EciI GGCGGA 1 cut(s) 427
Eco47I GGWCC 1 cut(s) 234
Eco57I CTGAAG 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 7
FaeI CATG 2 cut(s) 140, 158
FaiI YATR 3 cut(s) 138, 156, 504
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FaqI GGGAC 2 cut(s) 297, 380
FatI CATG 2 cut(s) 136, 154
FauI CCCGC 2 cut(s) 252, 373
Fnu4HI GCNGC 6 cut(s) 199, 289, 386, 446, 466, 476
FokI GGATG 2 cut(s) 441, 451
Fsp4HI GCNGC 6 cut(s) 199, 289, 386, 446, 466, 476
GlaI GCGC 1 cut(s) 449
GluI GCNGC 6 cut(s) 199, 289, 386, 446, 466, 476
GsaI CCCAGC 2 cut(s) 17, 510
GsuI CTGGAG 1 cut(s) 454
HapII CCGG 1 cut(s) 78
HgaI GACGC 2 cut(s) 294, 399
HhaI GCGC 1 cut(s) 450
Hin1I GRCGYC 1 cut(s) 391
Hin1II CATG 2 cut(s) 140, 158
Hin6I GCGC 1 cut(s) 448
HinP1I GCGC 1 cut(s) 448
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 2 cut(s) 176, 215
HpaII CCGG 1 cut(s) 78
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 2 cut(s) 36, 419
Hpy188III TCNNGA 4 cut(s) 149, 212, 237, 337
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 393
HpyCH4III ACNGT 4 cut(s) 45, 119, 251, 522
HpyCH4V TGCA 3 cut(s) 267, 320, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
Hsp92I GRCGYC 1 cut(s) 391
Hsp92II CATG 2 cut(s) 140, 158
HspAI GCGC 1 cut(s) 448
Kzo9I GATC 2 cut(s) 414, 515
LpnPI CCDG 9 cut(s) 21, 46, 91, 250, 266, 386, 418, 492, 505
Lsp1109I GCAGC 3 cut(s) 210, 397, 487
LweI GCATC 2 cut(s) 307, 419
MaeIII GTNAC 2 cut(s) 166, 298
MalI GATC 2 cut(s) 416, 517
MboI GATC 2 cut(s) 414, 515
MboII GAAGA 2 cut(s) 23, 340
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 1 cut(s) 492
MluCI AATT 2 cut(s) 190, 228
MmeI TCCRAC 2 cut(s) 382, 406
MnlI CCTC 2 cut(s) 394, 429
MseI TTAA 3 cut(s) 123, 221, 305
MspI CCGG 1 cut(s) 78
MspR9I CCNGG 1 cut(s) 9
MvaI CCWGG 1 cut(s) 9
MvnI CGCG 2 cut(s) 288, 450
MwoI GCNNNNNNNGC 1 cut(s) 10
NdeII GATC 2 cut(s) 414, 515
NlaIII CATG 2 cut(s) 140, 158
NlaIV GGNNCC 2 cut(s) 416, 489
NmuCI GTSAC 2 cut(s) 166, 298
NspI RCATGY 1 cut(s) 158
PciI ACATGT 1 cut(s) 154
PcsI WCGNNNNNNNCGW 1 cut(s) 335
PfeI GAWTC 2 cut(s) 176, 215
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 6 cut(s) 200, 290, 387, 447, 467, 477
PscI ACATGT 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 305
Psp6I CCWGG 1 cut(s) 7
PspFI CCCAGC 2 cut(s) 13, 506
PspGI CCWGG 1 cut(s) 7
PspN4I GGNNCC 2 cut(s) 416, 489
PspPI GGNCC 1 cut(s) 234
PsuI RGATCY 1 cut(s) 414
PsyI GACNNNGTC 1 cut(s) 164
SaqAI TTAA 3 cut(s) 123, 221, 305
SatI GCNGC 6 cut(s) 199, 289, 386, 446, 466, 476
Sau3AI GATC 2 cut(s) 414, 515
Sau96I GGNCC 1 cut(s) 234
ScrFI CCNGG 1 cut(s) 9
SduI GDGCHC 1 cut(s) 492
SetI ASST 3 cut(s) 10, 70, 405
SfaNI GCATC 2 cut(s) 307, 419
SfcI CTRYAG 1 cut(s) 396
SinI GGWCC 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 292
SmoI CTYRAG 1 cut(s) 292
Sse9I AATT 2 cut(s) 190, 228
SsiI CCGC 7 cut(s) 259, 288, 380, 412, 445, 450, 465
StyD4I CCNGG 1 cut(s) 7
TaaI ACNGT 4 cut(s) 45, 119, 251, 522
TasI AATT 2 cut(s) 190, 228
TauI GCSGC 3 cut(s) 291, 448, 468
TfiI GAWTC 2 cut(s) 176, 215
Tru1I TTAA 3 cut(s) 123, 221, 305
Tru9I TTAA 3 cut(s) 123, 221, 305
TscAI CASTG 2 cut(s) 256, 525
TseFI GTSAC 2 cut(s) 166, 298
TseI GCWGC 3 cut(s) 198, 385, 475
Tsp45I GTSAC 2 cut(s) 166, 298
TspDTI ATGAA 2 cut(s) 40, 125
TspRI CASTG 2 cut(s) 256, 525
Tth111I GACNNNGTC 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 234
VspI ATTAAT 1 cut(s) 305
XapI RAATTY 1 cut(s) 190
XceI RCATGY 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.