Rh4CG333300

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
N/A
Physical Location & Seq
Forward (+)
59417110 .. 59417759
650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG333300.1

Sequence Viewer

Length: 537 bp
ATGATATATGCACGAATGTTGGTTGATTATGTTCTCTCTTGGATGCACTTAGTCAAACATGTATTTGCTCTTAACAGGTATTATGCTAGTCAGCAATTGACCGAAAAAAGTGATGTATATAGTTTTGGGGTTGTTCTGCTAGAACTGATTTCAGGAAAAAAGCCTGTGTCACCAGAAGACTTTGGTCCTGAATTGAACATTGTTCACTGGGCTAAATCCTTAATTCGTAAAGGGGATGTAGTAAGCATCATGGATCCATTTCTAGAAGAGGGTGTCAAGATCGAGTCCATTTGGAGAATTGCTGAAGTTGCAATACAATGTGTTGAGCAACATGGAGTTTCTCGGCCGAGAATGCAGGAGATCATTGTGGCCATACAAGAGGCTATGAAGATTGAAAAAGGCAGTGAAGCCAACCAGAGAATATCTTCAAGTAGTTCTTCCAGAGCACAATCTTCCCGGAAAACTTTACTCACAAGCTTCCTTGAAATCGAGAGCCCTGACATATCAAAAGATTGCCTAACTCCATCTGCAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_4 LRR_8 Malectin_like Pkinase_Tyr

Protein Analysis

178

Amino Acids

20.11

Weight (kDa)

6.43

Isoelectric Point (pI)

56.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 30 - 122 3.8e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 248, 261
AcoI YGGCCR 2 cut(s) 344, 369
AcuI CTGAAG 1 cut(s) 324
AflIII ACRYGT 1 cut(s) 58
AgsI TTSAA 4 cut(s) 196, 395, 429, 485
AluBI AGCT 1 cut(s) 477
AluI AGCT 1 cut(s) 477
Alw21I GWGCWC 1 cut(s) 448
AlwI GGATC 2 cut(s) 248, 261
AoxI GGCC 2 cut(s) 344, 369
Asp700I GAANNNNTTC 1 cut(s) 424
AspS9I GGNCC 1 cut(s) 185
AsuC2I CCSGG 1 cut(s) 457
AsuHPI GGTGA 1 cut(s) 162
AvaII GGWCC 1 cut(s) 185
BalI TGGCCA 1 cut(s) 371
BamHI GGATCC 1 cut(s) 253
BanII GRGCYC 1 cut(s) 497
BarI GAAGNNNNNNTAC 2 cut(s) 297, 329
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 448
BccI CCATC 1 cut(s) 532
BcnI CCSGG 1 cut(s) 457
BfaI CTAG 3 cut(s) 87, 140, 263
Bme1390I CCNGG 1 cut(s) 457
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 1 cut(s) 457
BmrI ACTGGG 1 cut(s) 217
BmsI GCATC 2 cut(s) 33, 255
BmuI ACTGGG 1 cut(s) 217
BoxI GACNNNNGTC 1 cut(s) 183
BpiI GAAGAC 1 cut(s) 183
BpuMI CCSGG 1 cut(s) 457
Bse1I ACTGG 1 cut(s) 212
BseGI GGATG 2 cut(s) 48, 241
BseNI ACTGG 1 cut(s) 212
BseX3I CGGCCG 1 cut(s) 344
Bsh1285I CGRYCG 1 cut(s) 347
BshFI GGCC 2 cut(s) 346, 371
BsiEI CGRYCG 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 448
BsiSI CCGG 1 cut(s) 457
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 2 cut(s) 346, 371
Bsp1286I GDGCHC 2 cut(s) 448, 497
Bsp143I GATC 3 cut(s) 253, 279, 360
BspANI GGCC 2 cut(s) 346, 371
BspLI GGNNCC 1 cut(s) 255
BspPI GGATC 2 cut(s) 248, 261
BsrI ACTGG 1 cut(s) 212
BssMI GATC 3 cut(s) 253, 279, 360
Bst6I CTCTTC 1 cut(s) 261
BstDEI CTNAG 1 cut(s) 49
BstF5I GGATG 2 cut(s) 48, 241
BstKTI GATC 3 cut(s) 256, 282, 363
BstMBI GATC 3 cut(s) 253, 279, 360
BstMCI CGRYCG 1 cut(s) 347
BstMWI GCNNNNNNNGC 2 cut(s) 308, 352
BstNSI RCATGY 1 cut(s) 62
BstPAI GACNNNNGTC 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 455
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BstZI CGGCCG 1 cut(s) 344
BsuRI GGCC 2 cut(s) 346, 371
BtsCI GGATG 2 cut(s) 48, 241
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 2 cut(s) 205, 409
Cfr13I GGNCC 1 cut(s) 185
CviAII CATG 3 cut(s) 59, 250, 332
CviJI RGCY 8 cut(s) 163, 212, 346, 371, 383, 410, 477, 495
CviKI_1 RGCY 8 cut(s) 163, 212, 346, 371, 383, 410, 477, 495
DdeI CTNAG 1 cut(s) 49
DpnI GATC 3 cut(s) 255, 281, 362
DpnII GATC 3 cut(s) 253, 279, 360
EaeI YGGCCR 2 cut(s) 344, 369
EagI CGGCCG 1 cut(s) 344
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
EclXI CGGCCG 1 cut(s) 344
Eco24I GRGCYC 1 cut(s) 497
Eco47I GGWCC 1 cut(s) 185
Eco52I CGGCCG 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 324
EcoT38I GRGCYC 1 cut(s) 497
FaeI CATG 3 cut(s) 62, 253, 335
FalI AAGNNNNNCTT 2 cut(s) 421, 453
FatI CATG 3 cut(s) 58, 249, 331
FokI GGATG 2 cut(s) 55, 248
FriOI GRGCYC 1 cut(s) 497
FspBI CTAG 3 cut(s) 87, 140, 263
HaeIII GGCC 2 cut(s) 346, 371
HapII CCGG 1 cut(s) 457
Hin1II CATG 3 cut(s) 62, 253, 335
HindIII AAGCTT 1 cut(s) 475
HinfI GANTC 1 cut(s) 284
HpaII CCGG 1 cut(s) 457
HphI GGTGA 1 cut(s) 162
Hpy166II GTNNAC 1 cut(s) 205
Hpy188III TCNNGA 6 cut(s) 153, 188, 263, 277, 441, 490
Hpy8I GTNNAC 1 cut(s) 205
HpyCH4V TGCA 5 cut(s) 11, 46, 311, 355, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 352
HpyF3I CTNAG 1 cut(s) 49
Hsp92II CATG 3 cut(s) 62, 253, 335
Kzo9I GATC 3 cut(s) 253, 279, 360
LweI GCATC 2 cut(s) 33, 255
MaeI CTAG 3 cut(s) 87, 140, 263
MaeIII GTNAC 1 cut(s) 168
MalI GATC 3 cut(s) 255, 281, 362
MboI GATC 3 cut(s) 253, 279, 360
MboII GAAGA 6 cut(s) 188, 278, 400, 417, 429, 444
MfeI CAATTG 1 cut(s) 95
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 2 cut(s) 448, 497
MlsI TGGCCA 1 cut(s) 371
MluCI AATT 4 cut(s) 95, 191, 222, 297
MluNI TGGCCA 1 cut(s) 371
MlyI GAGTC 1 cut(s) 293
MnlI CCTC 2 cut(s) 262, 373
Mox20I TGGCCA 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 424
MscI TGGCCA 1 cut(s) 371
MseI TTAA 2 cut(s) 72, 221
Msp20I TGGCCA 1 cut(s) 371
MspI CCGG 1 cut(s) 457
MspR9I CCNGG 1 cut(s) 457
MunI CAATTG 1 cut(s) 95
Mva1269I GAATGC 1 cut(s) 357
MwoI GCNNNNNNNGC 2 cut(s) 308, 352
NciI CCSGG 1 cut(s) 457
NdeII GATC 3 cut(s) 253, 279, 360
NlaIII CATG 3 cut(s) 62, 253, 335
NlaIV GGNNCC 1 cut(s) 255
NmeAIII GCCGAG 2 cut(s) 322, 372
NmuCI GTSAC 1 cut(s) 168
NspI RCATGY 1 cut(s) 62
PciI ACATGT 1 cut(s) 58
PctI GAATGC 1 cut(s) 357
PdmI GAANNNNTTC 1 cut(s) 424
PfoI TCCNGGA 1 cut(s) 455
PleI GAGTC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 292
PscI ACATGT 1 cut(s) 58
PshAI GACNNNNGTC 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 1 cut(s) 185
PsuI RGATCY 1 cut(s) 253
SaqAI TTAA 2 cut(s) 72, 221
Sau3AI GATC 3 cut(s) 253, 279, 360
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 1 cut(s) 457
SduI GDGCHC 2 cut(s) 448, 497
SetI ASST 2 cut(s) 80, 479
SfaNI GCATC 2 cut(s) 33, 255
SinI GGWCC 1 cut(s) 185
Sse9I AATT 4 cut(s) 95, 191, 222, 297
SspMI CTAG 3 cut(s) 87, 140, 263
StyD4I CCNGG 1 cut(s) 455
TaqI TCGA 2 cut(s) 282, 489
TaqII GACCGA 1 cut(s) 116
TasI AATT 4 cut(s) 95, 191, 222, 297
Tru1I TTAA 2 cut(s) 72, 221
Tru9I TTAA 2 cut(s) 72, 221
TscAI CASTG 2 cut(s) 212, 409
TseFI GTSAC 1 cut(s) 168
Tsp45I GTSAC 1 cut(s) 168
TspDTI ATGAA 1 cut(s) 401
TspRI CASTG 2 cut(s) 212, 409
VpaK11BI GGWCC 1 cut(s) 185
XbaI TCTAGA 1 cut(s) 262
XceI RCATGY 1 cut(s) 62
XmnI GAANNNNTTC 1 cut(s) 424
XspI CTAG 3 cut(s) 87, 140, 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.