Rh5AG540100
WRKY Family

WRKY DNA -binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
N/A
Physical Location & Seq
Reverse (-)
92797937 .. 92799263
1327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG540100.1

Sequence Viewer

Length: 288 bp
ATGAATGGTGATGGATCGATCAGGAGCTATTTCAAGTGCCAAAACAGCAATTGTAGAGCCAAGAAGCGAGCCGAGTGGTCGAGCTCAGAGCCTGATGAACTTCGAGTAGTGTATGAAGGGGTGCACAACCACACCGATAATAATGCCTCATCTGCAGGATCGGCTTCACATTCACAGCAGCCGGATTCCTCATCATCTTCAAATCCGAGTACTGATGCAAACCGGTATGACTTGTACACACAAGTTTTTGGTAATCAATCCTTCTCCAAGTGGGATCAAAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

95

Amino Acids

10.56

Weight (kDa)

6.25

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 4 - 44 1.7e-11 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 22, 166, 282
AfaI GTAC 2 cut(s) 211, 236
AgeI ACCGGT 1 cut(s) 222
AgsI TTSAA 2 cut(s) 34, 201
AluBI AGCT 2 cut(s) 27, 84
AluI AGCT 2 cut(s) 27, 84
Alw21I GWGCWC 2 cut(s) 86, 126
Alw44I GTGCAC 1 cut(s) 122
AlwI GGATC 3 cut(s) 22, 166, 282
AlwNI CAGNNNCTG 1 cut(s) 92
ApaLI GTGCAC 1 cut(s) 122
ApeKI GCWGC 1 cut(s) 178
AsiGI ACCGGT 1 cut(s) 222
AsuHPI GGTGA 1 cut(s) 20
BaeGI GKGCMC 1 cut(s) 126
BanII GRGCYC 1 cut(s) 86
Bbv12I GWGCWC 2 cut(s) 86, 126
BbvI GCAGC 1 cut(s) 190
BccI CCATC 1 cut(s) 5
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BfmI CTRYAG 1 cut(s) 153
BisI GCNGC 1 cut(s) 179
BlsI GCNGC 1 cut(s) 180
BmcAI AGTACT 1 cut(s) 211
BmsI GCATC 1 cut(s) 205
Bsa29I ATCGAT 1 cut(s) 17
BsaWI WCCGGW 1 cut(s) 222
Bse118I RCCGGY 1 cut(s) 222
BseCI ATCGAT 1 cut(s) 17
BseMII CTCAG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 126
BseXI GCAGC 1 cut(s) 190
BshTI ACCGGT 1 cut(s) 222
BshVI ATCGAT 1 cut(s) 17
BsiHKAI GWGCWC 2 cut(s) 86, 126
BsiSI CCGG 2 cut(s) 182, 223
Bsp1286I GDGCHC 2 cut(s) 86, 126
Bsp1407I TGTACA 1 cut(s) 234
Bsp143I GATC 4 cut(s) 14, 18, 158, 274
BspCNI CTCAG 1 cut(s) 98
BspDI ATCGAT 1 cut(s) 17
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 3 cut(s) 22, 166, 282
BsrFI RCCGGY 1 cut(s) 222
BsrGI TGTACA 1 cut(s) 234
BssAI RCCGGY 1 cut(s) 222
BssMI GATC 4 cut(s) 14, 18, 158, 274
BstAUI TGTACA 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 85
BstKTI GATC 4 cut(s) 17, 21, 161, 277
BstMBI GATC 4 cut(s) 14, 18, 158, 274
BstMWI GCNNNNNNNGC 3 cut(s) 45, 152, 161
BstSFI CTRYAG 1 cut(s) 153
BstSLI GKGCMC 1 cut(s) 126
BstV1I GCAGC 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 17
BsuTUI ATCGAT 1 cut(s) 17
Cac8I GCNNGC 1 cut(s) 69
CaiI CAGNNNCTG 1 cut(s) 92
Cfr10I RCCGGY 1 cut(s) 222
ClaI ATCGAT 1 cut(s) 17
Csp6I GTAC 2 cut(s) 210, 235
CspAI ACCGGT 1 cut(s) 222
CviJI RGCY 7 cut(s) 27, 59, 71, 84, 91, 164, 181
CviKI_1 RGCY 7 cut(s) 27, 59, 71, 84, 91, 164, 181
CviQI GTAC 2 cut(s) 210, 235
DdeI CTNAG 1 cut(s) 85
DpnI GATC 4 cut(s) 16, 20, 160, 276
DpnII GATC 4 cut(s) 14, 18, 158, 274
Ecl136II GAGCTC 1 cut(s) 84
Eco24I GRGCYC 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 84
EcoT38I GRGCYC 1 cut(s) 86
FaiI YATR 2 cut(s) 114, 228
Fnu4HI GCNGC 1 cut(s) 179
FriOI GRGCYC 1 cut(s) 86
Fsp4HI GCNGC 1 cut(s) 179
GluI GCNGC 1 cut(s) 179
HapII CCGG 2 cut(s) 182, 223
HinfI GANTC 1 cut(s) 185
HpaII CCGG 2 cut(s) 182, 223
HphI GGTGA 1 cut(s) 20
Hpy166II GTNNAC 2 cut(s) 124, 237
Hpy188I TCNGA 2 cut(s) 88, 207
Hpy188III TCNNGA 1 cut(s) 22
Hpy8I GTNNAC 2 cut(s) 124, 237
HpyAV CCTTC 2 cut(s) 110, 271
HpyCH4V TGCA 3 cut(s) 124, 155, 218
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 152, 161
HpyF3I CTNAG 1 cut(s) 85
Kzo9I GATC 4 cut(s) 14, 18, 158, 274
LmnI GCTCC 1 cut(s) 24
LpnPI CCDG 5 cut(s) 7, 105, 141, 195, 236
Lsp1109I GCAGC 1 cut(s) 190
LweI GCATC 1 cut(s) 205
MalI GATC 4 cut(s) 16, 20, 160, 276
MboI GATC 4 cut(s) 14, 18, 158, 274
MboII GAAGA 1 cut(s) 189
MfeI CAATTG 1 cut(s) 49
MhlI GDGCHC 2 cut(s) 86, 126
MluCI AATT 1 cut(s) 49
MnlI CCTC 2 cut(s) 157, 199
MspI CCGG 2 cut(s) 182, 223
MunI CAATTG 1 cut(s) 49
MwoI GCNNNNNNNGC 3 cut(s) 45, 152, 161
NdeII GATC 4 cut(s) 14, 18, 158, 274
NmeAIII GCCGAG 1 cut(s) 97
PfeI GAWTC 1 cut(s) 185
PinAI ACCGGT 1 cut(s) 222
PkrI GCNGC 1 cut(s) 180
Psp124BI GAGCTC 1 cut(s) 86
PstI CTGCAG 1 cut(s) 157
PstNI CAGNNNCTG 1 cut(s) 92
RsaI GTAC 2 cut(s) 211, 236
RsaNI GTAC 2 cut(s) 210, 235
SacI GAGCTC 1 cut(s) 86
SatI GCNGC 1 cut(s) 179
Sau3AI GATC 4 cut(s) 14, 18, 158, 274
ScaI AGTACT 1 cut(s) 211
SduI GDGCHC 2 cut(s) 86, 126
SetI ASST 2 cut(s) 29, 86
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 1 cut(s) 153
Sse9I AATT 1 cut(s) 49
SstI GAGCTC 1 cut(s) 86
TaqI TCGA 3 cut(s) 17, 80, 103
TasI AATT 1 cut(s) 49
TatI WGTACW 2 cut(s) 209, 234
TfiI GAWTC 1 cut(s) 185
TseI GCWGC 1 cut(s) 178
TspDTI ATGAA 3 cut(s) 17, 111, 129
VneI GTGCAC 1 cut(s) 122
ZrmI AGTACT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.