Rh5CG282200

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
N/A
Physical Location & Seq
Forward (+)
30832332 .. 30833842
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG282200.1

Sequence Viewer

Length: 1377 bp
ATGCCTTTTCTTTTCTCAGCAATTATCTTCTCCCTTCTAAGTACCTACACTACCAGTATACAAGACATCATTACCGTTAATCCAAGAGTATCAATCAAAGATGGAGGGAGTACCCTAGTTTCAGCTGGAGGAAGCTTCGAATTGGGATTCTTTAGCCCAGGTAATTCGAAGAGTCGATACGTGGGAATATGGTACAATAACATATCTCCTCAGAAAGTTGTATGGGTGTGCAACAAAGAAACTCCCCTTACCGATCACTCGGGAGCTTTGAATCTCACTAGCCAAGGAGTTCTCGTTCTGTTCAATGGCACAAATAGTATTGTCTGGTCATCATCCAACAAGACTTCAAGCAACATAAGTAACCCAGTTGCTCAACTCTTGGAGTCGGGCAATTTCGTGGTGAAAGATGGATCAGAGGAGGACTCAAAAGTTCTGTGGCAGAGCTTTGACTATCCAAGTGACACCTTACTACCAGGAATGAAGATCGGATGGGACTTAAAAACCGGCCTAAACAGGTTTCTGTCATCTTGGAAAGAAGCAGAAGATCCTGCTCCAGGACAATTTTCATTTTCTTTAGATCGTAGTGGGTATCCCCAACTAGTTCTTAGGAATGGTTCCATAGCACATTACAGGTTGGGGTCATGGAATGGCCTTGGCTTCACAGGAAGTCCTCGACTTGGATCTCAGAACCAGTTGTTCAAACTTGACTTTGTGTCAAATGAGAATGAAGTATACTACAAATATGAGCTCCTGAGCACTTCAGTTGAATCAAGATTAGTGCTCAACAGGTCAGGCGATTTGCAACGCTTCATGTGGAGTGGCACAACAAATATCAATCGCGTCATTTACTCAGCTCCAGCAGATCAGTGTGACGCTTATAATGTGTGCGGTGCATTTGCTCTCTGCAGTGTTGATTCTCCTCAATTATGTGCATGCTTACAAGGTTTTGCACCAAATTCTTCAACGTCCAGCAGGTGTCTTCGTAGAACTCCATTGAGTTGCAATGATTACCAAGATGGCTTCAAGAAGGTCACAAATGTTAAATTGCCGGACACATCTTTCTCCTGGTTTAACTTGACAATGGGCCTTGAGGACTGTAAGAAAATTTGTTTGAAGAACTGCAATTGCACTGCTTATGCAAATTTGGATGTAAGGGAAGGAGGAAGTGGCTGTTTGCTCTGGTTTGATGAACTCAATGACATACACGAATTCGCTACTGGTGGGCAAGACCTATTTGTAAAGATGGCTACCTCAGAATTAGGTAACTTTCTTCTCAAGTTTTGTTCGTTTTTTAAGCTATGCATACACCAAGATCCACATTTACATCTTGTCTCAACTTTTTCCCACATCATTACACATAAAGATGATGTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

458

Amino Acids

50.7

Weight (kDa)

5.93

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 178 1.6e-37 D-mannose binding lectin
S_locus_glycop PF00954 210 - 319 6.2e-28 S-locus glycoprotein domain
PAN_2 PF08276 334 - 401 6.1e-22 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 879
AarI CACCTGC 1 cut(s) 963
Acc36I ACCTGC 1 cut(s) 963
AccI GTMKAC 2 cut(s) 58, 732
AccII CGCG 1 cut(s) 840
AciI CCGC 1 cut(s) 888
AclWI GGATC 4 cut(s) 418, 539, 688, 1307
AcsI RAATTY 4 cut(s) 955, 1104, 1141, 1208
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 3 cut(s) 43, 112, 194
AfiI CCNNNNNNNGG 2 cut(s) 554, 677
AgsI TTSAA 8 cut(s) 271, 304, 348, 700, 767, 963, 1024, 1114
AhdI GACNNNNNGTC 1 cut(s) 712
AhlI ACTAGT 1 cut(s) 598
AjnI CCWGG 4 cut(s) 157, 472, 553, 1064
AloI GAACNNNNNNTCC 2 cut(s) 279, 311
AluBI AGCT 7 cut(s) 125, 135, 266, 444, 748, 854, 1297
AluI AGCT 7 cut(s) 125, 135, 266, 444, 748, 854, 1297
Alw21I GWGCWC 3 cut(s) 750, 758, 783
Alw26I GTCTC 1 cut(s) 1336
AlwI GGATC 4 cut(s) 418, 539, 688, 1307
Ama87I CYCGRG 1 cut(s) 259
AoxI GGCC 3 cut(s) 505, 649, 1084
ApoI RAATTY 4 cut(s) 955, 1104, 1141, 1208
AspS9I GGNCC 1 cut(s) 1084
AsuHPI GGTGA 1 cut(s) 412
AsuII TTCGAA 2 cut(s) 138, 167
AvaI CYCGRG 1 cut(s) 259
BanII GRGCYC 1 cut(s) 750
BarI GAAGNNNNNNTAC 2 cut(s) 161, 193
BbsI GAAGAC 1 cut(s) 971
Bbv12I GWGCWC 3 cut(s) 750, 758, 783
BccI CCATC 5 cut(s) 95, 401, 483, 1010, 1237
BciT130I CCWGG 4 cut(s) 159, 474, 555, 1066
BciVI GTATCC 1 cut(s) 600
BcoDI GTCTC 1 cut(s) 1336
BcuI ACTAGT 1 cut(s) 598
BfaI CTAG 3 cut(s) 116, 279, 599
BfmI CTRYAG 1 cut(s) 904
BfuAI ACCTGC 1 cut(s) 963
BfuI GTATCC 1 cut(s) 600
Bme1390I CCNGG 4 cut(s) 159, 474, 555, 1066
BmeRI GACNNNNNGTC 1 cut(s) 712
BmeT110I CYCGRG 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 1084
BmiI GGNNCC 1 cut(s) 616
BmrFI CCNGG 4 cut(s) 159, 474, 555, 1066
BmrI ACTGGG 1 cut(s) 359
BmuI ACTGGG 1 cut(s) 359
BpiI GAAGAC 1 cut(s) 971
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpmI CTGGAG 3 cut(s) 147, 537, 840
Bpu10I CCTNAGC 1 cut(s) 752
Bpu14I TTCGAA 2 cut(s) 138, 167
BpuEI CTTGAG 2 cut(s) 1109, 1259
BsaAI YACGTR 1 cut(s) 181
BsaJI CCNNGG 3 cut(s) 157, 283, 652
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 2 cut(s) 554, 677
Bse118I RCCGGY 1 cut(s) 503
Bse1I ACTGG 4 cut(s) 54, 365, 691, 1222
Bse3DI GCAATG 1 cut(s) 1009
BseBI CCWGG 4 cut(s) 159, 474, 555, 1066
BseDI CCNNGG 3 cut(s) 157, 283, 652
BseGI GGATG 3 cut(s) 332, 494, 1153
BseLI CCNNNNNNNGG 2 cut(s) 554, 677
BseMI GCAATG 1 cut(s) 1009
BseMII CTCAG 6 cut(s) 30, 224, 698, 743, 864, 1266
BseNI ACTGG 4 cut(s) 54, 365, 691, 1222
BseRI GAGGAG 3 cut(s) 198, 431, 909
Bsh1236I CGCG 1 cut(s) 840
BshFI GGCC 3 cut(s) 507, 651, 1086
BsiHKAI GWGCWC 3 cut(s) 750, 758, 783
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 2 cut(s) 504, 1049
BslFI GGGAC 1 cut(s) 506
BslI CCNNNNNNNGG 2 cut(s) 554, 677
BsmAI GTCTC 1 cut(s) 1336
BsmFI GGGAC 1 cut(s) 506
BsnI GGCC 3 cut(s) 507, 651, 1086
BsoBI CYCGRG 1 cut(s) 259
Bsp119I TTCGAA 2 cut(s) 138, 167
Bsp1286I GDGCHC 3 cut(s) 750, 758, 783
Bsp143I GATC 8 cut(s) 253, 410, 483, 544, 577, 680, 862, 1312
BspACI CCGC 1 cut(s) 888
BspANI GGCC 3 cut(s) 507, 651, 1086
BspCNI CTCAG 6 cut(s) 29, 223, 697, 744, 863, 1265
BspFNI CGCG 1 cut(s) 840
BspLI GGNNCC 1 cut(s) 616
BspMAI CTGCAG 1 cut(s) 908
BspMI ACCTGC 1 cut(s) 963
BspPI GGATC 4 cut(s) 418, 539, 688, 1307
BspT104I TTCGAA 2 cut(s) 138, 167
BsrDI GCAATG 1 cut(s) 1009
BsrFI RCCGGY 1 cut(s) 503
BsrI ACTGG 4 cut(s) 54, 365, 691, 1222
BssAI RCCGGY 1 cut(s) 503
BssECI CCNNGG 3 cut(s) 157, 283, 652
BssMI GATC 8 cut(s) 253, 410, 483, 544, 577, 680, 862, 1312
BssNAI GTATAC 2 cut(s) 59, 733
BssT1I CCWWGG 2 cut(s) 283, 652
Bst1107I GTATAC 2 cut(s) 59, 733
Bst2UI CCWGG 4 cut(s) 159, 474, 555, 1066
Bst4CI ACNGT 2 cut(s) 76, 1097
Bst6I CTCTTC 1 cut(s) 164
BstBAI YACGTR 1 cut(s) 181
BstBI TTCGAA 2 cut(s) 138, 167
BstC8I GCNNGC 1 cut(s) 934
BstDEI CTNAG 8 cut(s) 16, 38, 210, 605, 684, 752, 850, 1252
BstENI CCTNNNNNAGG 1 cut(s) 552
BstF5I GGATG 3 cut(s) 332, 494, 1153
BstFNI CGCG 1 cut(s) 840
BstKTI GATC 8 cut(s) 256, 413, 486, 547, 580, 683, 865, 1315
BstMAI GTCTC 1 cut(s) 1336
BstMBI GATC 8 cut(s) 253, 410, 483, 544, 577, 680, 862, 1312
BstNI CCWGG 4 cut(s) 159, 474, 555, 1066
BstNSI RCATGY 1 cut(s) 936
BstSCI CCNGG 4 cut(s) 157, 472, 553, 1064
BstSFI CTRYAG 1 cut(s) 904
BstUI CGCG 1 cut(s) 840
BstV2I GAAGAC 1 cut(s) 971
BstX2I RGATCY 3 cut(s) 544, 680, 1312
BstYI RGATCY 3 cut(s) 544, 680, 1312
BstZ17I GTATAC 2 cut(s) 59, 733
BsuI GTATCC 1 cut(s) 600
BsuRI GGCC 3 cut(s) 507, 651, 1086
BtsCI GGATG 3 cut(s) 332, 494, 1153
BtsI GCAGTG 2 cut(s) 913, 1128
BtsIMutI CAGTG 3 cut(s) 872, 913, 1128
BveI ACCTGC 1 cut(s) 963
Cac8I GCNNGC 1 cut(s) 934
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 1 cut(s) 1084
CseI GACGC 2 cut(s) 829, 881
Csp6I GTAC 3 cut(s) 42, 111, 193
CviAII CATG 3 cut(s) 642, 811, 933
CviQI GTAC 3 cut(s) 42, 111, 193
DdeI CTNAG 8 cut(s) 16, 38, 210, 605, 684, 752, 850, 1252
DpnI GATC 8 cut(s) 255, 412, 485, 546, 579, 682, 864, 1314
DpnII GATC 8 cut(s) 253, 410, 483, 544, 577, 680, 862, 1312
DriI GACNNNNNGTC 1 cut(s) 712
Eam1104I CTCTTC 1 cut(s) 164
Eam1105I GACNNNNNGTC 1 cut(s) 712
EarI CTCTTC 1 cut(s) 164
Ecl136II GAGCTC 1 cut(s) 748
Eco130I CCWWGG 2 cut(s) 283, 652
Eco24I GRGCYC 1 cut(s) 750
Eco53kI GAGCTC 1 cut(s) 748
Eco57I CTGAAG 1 cut(s) 744
Eco88I CYCGRG 1 cut(s) 259
EcoICRI GAGCTC 1 cut(s) 748
EcoNI CCTNNNNNAGG 1 cut(s) 552
EcoRI GAATTC 1 cut(s) 1208
EcoRII CCWGG 4 cut(s) 157, 472, 553, 1064
EcoT14I CCWWGG 2 cut(s) 283, 652
EcoT22I ATGCAT 1 cut(s) 1304
EcoT38I GRGCYC 1 cut(s) 750
ErhI CCWWGG 2 cut(s) 283, 652
FaeI CATG 3 cut(s) 645, 814, 936
FaqI GGGAC 1 cut(s) 506
FatI CATG 3 cut(s) 641, 810, 932
FblI GTMKAC 2 cut(s) 58, 732
FokI GGATG 3 cut(s) 319, 501, 1160
FriOI GRGCYC 1 cut(s) 750
FspBI CTAG 3 cut(s) 116, 279, 599
GsuI CTGGAG 3 cut(s) 147, 537, 840
HaeIII GGCC 3 cut(s) 507, 651, 1086
HapII CCGG 2 cut(s) 504, 1049
HgaI GACGC 2 cut(s) 829, 881
Hin1II CATG 3 cut(s) 645, 814, 936
HindIII AAGCTT 1 cut(s) 133
HinfI GANTC 7 cut(s) 147, 172, 271, 383, 422, 767, 914
HpaII CCGG 2 cut(s) 504, 1049
HphI GGTGA 1 cut(s) 412
Hpy166II GTNNAC 2 cut(s) 59, 733
Hpy188I TCNGA 5 cut(s) 213, 415, 488, 687, 1255
Hpy188III TCNNGA 4 cut(s) 261, 751, 771, 1024
Hpy8I GTNNAC 2 cut(s) 59, 733
HpyAV CCTTC 3 cut(s) 44, 1021, 1151
HpyCH4III ACNGT 2 cut(s) 76, 1097
HpyCH4IV ACGT 2 cut(s) 180, 965
HpyF3I CTNAG 8 cut(s) 16, 38, 210, 605, 684, 752, 850, 1252
HpySE526I ACGT 2 cut(s) 180, 965
Hsp92II CATG 3 cut(s) 645, 814, 936
Kzo9I GATC 8 cut(s) 253, 410, 483, 544, 577, 680, 862, 1312
LmnI GCTCC 4 cut(s) 263, 556, 753, 859
MaeI CTAG 3 cut(s) 116, 279, 599
MaeII ACGT 2 cut(s) 180, 965
MaeIII GTNAC 5 cut(s) 359, 458, 869, 1030, 1262
MalI GATC 8 cut(s) 255, 412, 485, 546, 579, 682, 864, 1314
MboI GATC 8 cut(s) 253, 410, 483, 544, 577, 680, 862, 1312
MboII GAAGA 8 cut(s) 19, 181, 493, 554, 951, 971, 1126, 1262
MfeI CAATTG 1 cut(s) 1123
MflI RGATCY 3 cut(s) 544, 680, 1312
MhlI GDGCHC 3 cut(s) 750, 758, 783
MlyI GAGTC 3 cut(s) 181, 392, 416
MmeI TCCRAC 1 cut(s) 360
Mph1103I ATGCAT 1 cut(s) 1304
MseI TTAA 5 cut(s) 78, 497, 1041, 1071, 1293
MslI CAYNNNNRTG 1 cut(s) 1362
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 2 cut(s) 504, 1049
MspR9I CCNGG 4 cut(s) 159, 474, 555, 1066
MunI CAATTG 1 cut(s) 1123
MvaI CCWGG 4 cut(s) 159, 474, 555, 1066
MvnI CGCG 1 cut(s) 840
NdeII GATC 8 cut(s) 253, 410, 483, 544, 577, 680, 862, 1312
NlaIII CATG 3 cut(s) 645, 814, 936
NlaIV GGNNCC 1 cut(s) 616
NmuCI GTSAC 3 cut(s) 458, 869, 1030
NsiI ATGCAT 1 cut(s) 1304
NspI RCATGY 1 cut(s) 936
NspV TTCGAA 2 cut(s) 138, 167
PaeI GCATGC 1 cut(s) 936
PaqCI CACCTGC 1 cut(s) 963
PfeI GAWTC 4 cut(s) 147, 271, 767, 914
PfoI TCCNGGA 1 cut(s) 553
PleI GAGTC 3 cut(s) 180, 391, 416
PpsI GAGTC 3 cut(s) 180, 391, 416
Ppu21I YACGTR 1 cut(s) 181
PsiI TTATAA 1 cut(s) 879
Psp124BI GAGCTC 1 cut(s) 750
Psp6I CCWGG 4 cut(s) 157, 472, 553, 1064
PspGI CCWGG 4 cut(s) 157, 472, 553, 1064
PspN4I GGNNCC 1 cut(s) 616
PspPI GGNCC 1 cut(s) 1084
PstI CTGCAG 1 cut(s) 908
PsuI RGATCY 3 cut(s) 544, 680, 1312
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 3 cut(s) 43, 112, 194
RsaNI GTAC 3 cut(s) 42, 111, 193
RseI CAYNNNNRTG 1 cut(s) 1362
SacI GAGCTC 1 cut(s) 750
SaqAI TTAA 5 cut(s) 78, 497, 1041, 1071, 1293
Sau3AI GATC 8 cut(s) 253, 410, 483, 544, 577, 680, 862, 1312
Sau96I GGNCC 1 cut(s) 1084
SchI GAGTC 3 cut(s) 181, 392, 416
ScrFI CCNGG 4 cut(s) 159, 474, 555, 1066
SduI GDGCHC 3 cut(s) 750, 758, 783
SfcI CTRYAG 1 cut(s) 904
SfuI TTCGAA 2 cut(s) 138, 167
SmiMI CAYNNNNRTG 1 cut(s) 1362
SmlI CTYRAG 2 cut(s) 1088, 1274
SmoI CTYRAG 2 cut(s) 1088, 1274
SpeI ACTAGT 1 cut(s) 598
SphI GCATGC 1 cut(s) 936
SsiI CCGC 1 cut(s) 888
SspMI CTAG 3 cut(s) 116, 279, 599
SstI GAGCTC 1 cut(s) 750
StyD4I CCNGG 4 cut(s) 157, 472, 553, 1064
StyI CCWWGG 2 cut(s) 283, 652
TaaI ACNGT 2 cut(s) 76, 1097
TaiI ACGT 2 cut(s) 183, 968
TaqI TCGA 4 cut(s) 138, 167, 175, 673
TfiI GAWTC 4 cut(s) 147, 271, 767, 914
Tru1I TTAA 5 cut(s) 78, 497, 1041, 1071, 1293
Tru9I TTAA 5 cut(s) 78, 497, 1041, 1071, 1293
TscAI CASTG 3 cut(s) 872, 913, 1135
TseFI GTSAC 3 cut(s) 458, 869, 1030
Tsp45I GTSAC 3 cut(s) 458, 869, 1030
TspDTI ATGAA 5 cut(s) 494, 555, 741, 799, 1203
TspRI CASTG 3 cut(s) 872, 913, 1135
XagI CCTNNNNNAGG 1 cut(s) 552
XapI RAATTY 4 cut(s) 955, 1104, 1141, 1208
XceI RCATGY 1 cut(s) 936
XmiI GTMKAC 2 cut(s) 58, 732
XspI CTAG 3 cut(s) 116, 279, 599
Zsp2I ATGCAT 1 cut(s) 1304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.