Rh5CG286500

leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
N/A
Physical Location & Seq
Forward (+)
31421253 .. 31424508
3256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG286500.1

Sequence Viewer

Length: 1092 bp
ATGAAAGGCGAGCCAGTTGGACTGATCATTGGGGTTTCTATAGGGGTAGTGATTGGAGTGCTTTTGGCAATTTCTGGGTTCTTCTGCATTAGGTACCATAGGAAGCGTATGCAAATAGGGAACAGCAGTTCTCGAAGGGCAGCAAATCTTCCTATTCGTACTAATGGCGCCGACACTTGTACAATGTTGTCAGACTCGACACTTGGTCCAGACTCACCTGTCAAATCTGAACGGACCGGTGGTTCCTTCTGGCTTGTTGAAGGATTTAAGAGGAGTAATGTAGTCTCAATGTCTGGAATACCCGAGTATTCTTACAAGGATCTGCAGAAAGCAACCTATAATTTTACAACATTGATAGGGCAAGGTGCATTTGGTCCTGTGTACAAAGCTCAGATGTTAACGGGTGAGACTGTTGCCGTTAAAGTGCTTGCAACTGATTCTAAGCAAGGGGAGAAAGAGTTTCAGACAGAGGTTAAGTTACTGGGAAGATTGCATCACAGGAACCTTGTGAACTTAATTGGATACTGTGCAGAAAAGGGCCAACATATGCTTATTTATGTCTACATGAGTAAAGGGAGTTTGGCCTCTCATTTGTATAATGAAAAGAATGAGCCATTGAGCTGGGATTTGAGGGTTCATATAGCTTTAGATGTTGCAAGGGGCTTGGAGTATCTTCATGATGGAGCAGTTCCTCCTGTAATACACCGGGATATCAAATCCAACAATATCCTGTTGGATGAATCCATGAGAGCCAGGGCGGCAATGAATACGGAGGGGAAATTTGGGTGGGAGGAAATTGTGGATTCCCGTCTTAATGGGAACTTTGATGTGCAAGAGCTCAACGAAGTGGCAGCTCTTTCATATAAATGTGTCAGCCGCTCCCCAAAAAAGCGGTCTTCCATGAGAGACATTGTACAAGTGCTATCACGAATGCTCAAAGTGAGGCACAATAGAAAGCACCATGCGAAATCATTGTCATCCACAGCAGAGGAAGTTATGATTGACGTGAACCAACCAGAAACCAAAATTCAAATGGCTGAACATCAAAGAGATGAGTCTATGGACAGCACAGCCGATACATATGATTTGTAG

Protein Analysis

363

Amino Acids

40.52

Weight (kDa)

8.86

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 113 - 248 1.5e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 115 - 251 3e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
Acc65I GGTACC 1 cut(s) 93
AccB1I GGYRCC 2 cut(s) 93, 167
AccBSI CCGCTC 1 cut(s) 879
AccI GTMKAC 1 cut(s) 561
AciI CCGC 3 cut(s) 758, 877, 892
AclWI GGATC 1 cut(s) 327
AcsI RAATTY 2 cut(s) 779, 1026
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 5 cut(s) 95, 160, 181, 383, 915
AgeI ACCGGT 1 cut(s) 236
AgsI TTSAA 2 cut(s) 260, 1031
AhdI GACNNNNNGTC 1 cut(s) 204
AjiI CACGTC 1 cut(s) 1006
AjnI CCWGG 1 cut(s) 752
AloI GAACNNNNNNTCC 4 cut(s) 112, 144, 226, 258
AluBI AGCT 5 cut(s) 389, 621, 644, 838, 854
AluI AGCT 5 cut(s) 389, 621, 644, 838, 854
Alw21I GWGCWC 1 cut(s) 840
Alw26I GTCTC 3 cut(s) 289, 401, 900
AlwI GGATC 1 cut(s) 327
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 2 cut(s) 538, 582
ApeKI GCWGC 2 cut(s) 140, 851
ApoI RAATTY 2 cut(s) 779, 1026
ArsI GACNNNNNNTTYG 4 cut(s) 878, 910, 959, 991
AsiGI ACCGGT 1 cut(s) 236
Asp718I GGTACC 1 cut(s) 93
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 4 cut(s) 206, 234, 374, 538
AsuC2I CCSGG 1 cut(s) 707
AsuHPI GGTGA 2 cut(s) 207, 416
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 3 cut(s) 206, 234, 374
BanI GGYRCC 2 cut(s) 93, 167
BanII GRGCYC 1 cut(s) 840
BbsI GAAGAC 1 cut(s) 888
Bbv12I GWGCWC 1 cut(s) 840
BbvI GCAGC 2 cut(s) 152, 863
BccI CCATC 1 cut(s) 674
BceAI ACGGC 1 cut(s) 401
BciT130I CCWGG 1 cut(s) 754
BciVI GTATCC 1 cut(s) 515
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 1 cut(s) 707
BcoDI GTCTC 3 cut(s) 289, 401, 900
BfmI CTRYAG 2 cut(s) 39, 323
BfoI RGCGCY 1 cut(s) 171
BfuI GTATCC 1 cut(s) 515
BglI GCCNNNNNGGC 1 cut(s) 758
BisI GCNGC 4 cut(s) 141, 759, 852, 877
BlsI GCNGC 4 cut(s) 142, 760, 853, 878
Bme1390I CCNGG 2 cut(s) 707, 754
Bme18I GGWCC 3 cut(s) 206, 234, 374
BmeRI GACNNNNNGTC 1 cut(s) 204
BmeT110I CYCGRG 1 cut(s) 302
BmgBI CACGTC 1 cut(s) 1006
BmgT120I GGNCC 4 cut(s) 206, 234, 374, 538
BmiI GGNNCC 4 cut(s) 95, 169, 244, 503
BmrFI CCNGG 2 cut(s) 707, 754
BmrI ACTGGG 1 cut(s) 491
BmsI GCATC 1 cut(s) 502
BmuI ACTGGG 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 888
BpuMI CCSGG 1 cut(s) 707
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 753
BsaWI WCCGGW 1 cut(s) 236
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 2 cut(s) 14, 486
Bse3DI GCAATG 1 cut(s) 768
BseBI CCWGG 1 cut(s) 754
BseDI CCNNGG 1 cut(s) 753
BseGI GGATG 2 cut(s) 742, 977
BseMI GCAATG 1 cut(s) 768
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 2 cut(s) 14, 486
BseRI GAGGAG 1 cut(s) 286
BseXI GCAGC 2 cut(s) 152, 863
BseYI CCCAGC 1 cut(s) 621
BsgI GTGCAG 1 cut(s) 549
BshFI GGCC 2 cut(s) 540, 584
BshNI GGYRCC 2 cut(s) 93, 167
BshTI ACCGGT 1 cut(s) 236
BsiHKAI GWGCWC 1 cut(s) 840
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 2 cut(s) 237, 706
BsmAI GTCTC 3 cut(s) 289, 401, 900
BsmI GAATGC 1 cut(s) 936
BsnI GGCC 2 cut(s) 540, 584
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 840
Bsp1407I TGTACA 3 cut(s) 179, 381, 913
Bsp143I GATC 2 cut(s) 24, 319
BspACI CCGC 3 cut(s) 758, 877, 892
BspANI GGCC 2 cut(s) 540, 584
BspCNI CTCAG 1 cut(s) 403
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 4 cut(s) 95, 169, 244, 503
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 1 cut(s) 327
BspT107I GGYRCC 2 cut(s) 93, 167
BsrBI CCGCTC 1 cut(s) 879
BsrDI GCAATG 1 cut(s) 768
BsrFI RCCGGY 1 cut(s) 236
BsrGI TGTACA 3 cut(s) 179, 381, 913
BsrI ACTGG 2 cut(s) 14, 486
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 753
BssMI GATC 2 cut(s) 24, 319
BssNI GRCGYC 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 754
Bst4CI ACNGT 2 cut(s) 412, 527
BstACI GRCGYC 1 cut(s) 168
BstAUI TGTACA 3 cut(s) 179, 381, 913
BstC8I GCNNGC 2 cut(s) 11, 429
BstDEI CTNAG 2 cut(s) 390, 441
BstF5I GGATG 2 cut(s) 742, 977
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 2 cut(s) 27, 322
BstMAI GTCTC 3 cut(s) 289, 401, 900
BstMBI GATC 2 cut(s) 24, 319
BstMWI GCNNNNNNNGC 1 cut(s) 758
BstNI CCWGG 1 cut(s) 754
BstSCI CCNGG 2 cut(s) 705, 752
BstSFI CTRYAG 2 cut(s) 39, 323
BstV1I GCAGC 2 cut(s) 152, 863
BstV2I GAAGAC 1 cut(s) 888
BstX2I RGATCY 1 cut(s) 319
BstXI CCANNNNNNTGG 1 cut(s) 621
BstYI RGATCY 1 cut(s) 319
BsuI GTATCC 1 cut(s) 515
BsuRI GGCC 2 cut(s) 540, 584
BtrI CACGTC 1 cut(s) 1006
BtsCI GGATG 2 cut(s) 742, 977
Cac8I GCNNGC 2 cut(s) 11, 429
CciI TCATGA 1 cut(s) 676
CfoI GCGC 1 cut(s) 170
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 4 cut(s) 206, 234, 374, 538
CpoI CGGWCCG 1 cut(s) 234
Csp6I GTAC 5 cut(s) 94, 159, 180, 382, 914
CspAI ACCGGT 1 cut(s) 236
CspI CGGWCCG 1 cut(s) 234
CviAII CATG 5 cut(s) 565, 677, 745, 901, 962
CviQI GTAC 5 cut(s) 94, 159, 180, 382, 914
DdeI CTNAG 2 cut(s) 390, 441
DinI GGCGCC 1 cut(s) 169
DpnI GATC 2 cut(s) 26, 321
DpnII GATC 2 cut(s) 24, 319
DrdI GACNNNNNNGTC 1 cut(s) 218
DriI GACNNNNNGTC 1 cut(s) 204
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1105I GACNNNNNGTC 1 cut(s) 204
Ecl136II GAGCTC 1 cut(s) 838
Eco24I GRGCYC 1 cut(s) 840
Eco32I GATATC 1 cut(s) 712
Eco47I GGWCC 3 cut(s) 206, 234, 374
Eco53kI GAGCTC 1 cut(s) 838
Eco88I CYCGRG 1 cut(s) 302
EcoICRI GAGCTC 1 cut(s) 838
EcoRII CCWGG 1 cut(s) 752
EcoRV GATATC 1 cut(s) 712
EcoT38I GRGCYC 1 cut(s) 840
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
FaeI CATG 5 cut(s) 568, 680, 748, 904, 965
FatI CATG 5 cut(s) 564, 676, 744, 900, 961
FauNDI CATATG 2 cut(s) 546, 1081
FbaI TGATCA 1 cut(s) 24
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 4 cut(s) 141, 759, 852, 877
FokI GGATG 2 cut(s) 749, 964
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 4 cut(s) 141, 759, 852, 877
GlaI GCGC 1 cut(s) 169
GluI GCNGC 4 cut(s) 141, 759, 852, 877
GsaI CCCAGC 1 cut(s) 625
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 2 cut(s) 540, 584
HapII CCGG 2 cut(s) 237, 706
HhaI GCGC 1 cut(s) 170
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 5 cut(s) 568, 680, 748, 904, 965
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HincII GTYRAC 1 cut(s) 399
HindII GTYRAC 1 cut(s) 399
HinfI GANTC 6 cut(s) 194, 212, 437, 740, 803, 1055
HpaI GTTAAC 1 cut(s) 399
HpaII CCGG 2 cut(s) 237, 706
HphI GGTGA 2 cut(s) 207, 416
Hpy166II GTNNAC 5 cut(s) 382, 399, 511, 562, 1009
Hpy188I TCNGA 4 cut(s) 193, 229, 393, 465
Hpy188III TCNNGA 5 cut(s) 132, 209, 294, 677, 927
Hpy8I GTNNAC 5 cut(s) 382, 399, 511, 562, 1009
HpyAV CCTTC 3 cut(s) 129, 254, 256
HpyCH4III ACNGT 2 cut(s) 412, 527
HpyCH4IV ACGT 1 cut(s) 1005
HpyCH4V TGCA 9 cut(s) 87, 112, 325, 368, 431, 493, 530, 656, 832
HpyF10VI GCNNNNNNNGC 1 cut(s) 758
HpyF3I CTNAG 2 cut(s) 390, 441
HpySE526I ACGT 1 cut(s) 1005
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 5 cut(s) 568, 680, 748, 904, 965
HspAI GCGC 1 cut(s) 168
KasI GGCGCC 1 cut(s) 167
KpnI GGTACC 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 24
KspAI GTTAAC 1 cut(s) 399
Kzo9I GATC 2 cut(s) 24, 319
LmnI GCTCC 2 cut(s) 683, 884
Lsp1109I GCAGC 2 cut(s) 152, 863
LweI GCATC 1 cut(s) 502
MaeII ACGT 1 cut(s) 1005
MaeIII GTNAC 1 cut(s) 477
MalI GATC 2 cut(s) 26, 321
MbiI CCGCTC 1 cut(s) 879
MboI GATC 2 cut(s) 24, 319
MboII GAAGA 5 cut(s) 73, 140, 498, 665, 888
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 1 cut(s) 840
MluCI AATT 6 cut(s) 69, 340, 516, 779, 795, 1026
Mly113I GGCGCC 1 cut(s) 168
MlyI GAGTC 3 cut(s) 188, 206, 1064
MmeI TCCRAC 2 cut(s) 714, 744
MnlI CCTC 9 cut(s) 264, 463, 595, 624, 702, 766, 784, 936, 982
MseI TTAA 6 cut(s) 267, 398, 420, 474, 515, 813
MslI CAYNNNNRTG 1 cut(s) 865
MspI CCGG 2 cut(s) 237, 706
MspR9I CCNGG 2 cut(s) 707, 754
Mva1269I GAATGC 1 cut(s) 936
MvaI CCWGG 1 cut(s) 754
MwoI GCNNNNNNNGC 1 cut(s) 758
NarI GGCGCC 1 cut(s) 168
NciI CCSGG 1 cut(s) 707
NdeI CATATG 2 cut(s) 546, 1081
NdeII GATC 2 cut(s) 24, 319
NlaIII CATG 5 cut(s) 568, 680, 748, 904, 965
NlaIV GGNNCC 4 cut(s) 95, 169, 244, 503
PagI TCATGA 1 cut(s) 676
PctI GAATGC 1 cut(s) 936
PfeI GAWTC 3 cut(s) 437, 740, 803
PinAI ACCGGT 1 cut(s) 236
PkrI GCNGC 4 cut(s) 142, 760, 853, 878
PleI GAGTC 3 cut(s) 188, 206, 1063
PluTI GGCGCC 1 cut(s) 171
PpsI GAGTC 3 cut(s) 188, 206, 1063
Psp124BI GAGCTC 1 cut(s) 840
Psp6I CCWGG 1 cut(s) 752
PspFI CCCAGC 1 cut(s) 621
PspGI CCWGG 1 cut(s) 752
PspN4I GGNNCC 4 cut(s) 95, 169, 244, 503
PspPI GGNCC 4 cut(s) 206, 234, 374, 538
PstI CTGCAG 1 cut(s) 327
PsuI RGATCY 1 cut(s) 319
RsaI GTAC 5 cut(s) 95, 160, 181, 383, 915
RsaNI GTAC 5 cut(s) 94, 159, 180, 382, 914
RseI CAYNNNNRTG 1 cut(s) 865
Rsr2I CGGWCCG 1 cut(s) 234
RsrII CGGWCCG 1 cut(s) 234
SacI GAGCTC 1 cut(s) 840
SaqAI TTAA 6 cut(s) 267, 398, 420, 474, 515, 813
SatI GCNGC 4 cut(s) 141, 759, 852, 877
Sau3AI GATC 2 cut(s) 24, 319
Sau96I GGNCC 4 cut(s) 206, 234, 374, 538
SchI GAGTC 3 cut(s) 188, 206, 1064
ScrFI CCNGG 2 cut(s) 707, 754
SduI GDGCHC 1 cut(s) 840
SfaNI GCATC 1 cut(s) 502
SfcI CTRYAG 2 cut(s) 39, 323
SfoI GGCGCC 1 cut(s) 169
SinI GGWCC 3 cut(s) 206, 234, 374
SmiMI CAYNNNNRTG 1 cut(s) 865
Sse9I AATT 6 cut(s) 69, 340, 516, 779, 795, 1026
SsiI CCGC 3 cut(s) 758, 877, 892
SspDI GGCGCC 1 cut(s) 167
SstI GAGCTC 1 cut(s) 840
StyD4I CCNGG 2 cut(s) 705, 752
TaaI ACNGT 2 cut(s) 412, 527
TaiI ACGT 1 cut(s) 1008
TaqI TCGA 2 cut(s) 133, 197
TasI AATT 6 cut(s) 69, 340, 516, 779, 795, 1026
TatI WGTACW 3 cut(s) 179, 381, 913
TauI GCSGC 2 cut(s) 761, 879
TfiI GAWTC 3 cut(s) 437, 740, 803
Tru1I TTAA 6 cut(s) 267, 398, 420, 474, 515, 813
Tru9I TTAA 6 cut(s) 267, 398, 420, 474, 515, 813
TseI GCWGC 2 cut(s) 140, 851
TspDTI ATGAA 7 cut(s) 17, 615, 626, 665, 753, 779, 849
TspGWI ACGGA 2 cut(s) 247, 785
VpaK11BI GGWCC 3 cut(s) 206, 234, 374
XapI RAATTY 2 cut(s) 779, 1026
XcmI CCANNNNNNNNNTGG 1 cut(s) 1030
XmiI GTMKAC 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.