Rh5DG195000

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
N/A
Physical Location & Seq
Reverse (-)
21621607 .. 21623188
1582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG195000.1

Sequence Viewer

Length: 1386 bp
ATGGAGGACGTTGCAGAATGGTATCTAAATGAACTGGTTGGTAGAAACTTGGTTAGCGTCAGCAGCAAGGAAACTGATGGACAAGTAAGGAGGTGTCGTGTTTCGAATCTTGTTCACCACTTCATCATTTCTAAACCTGAGAATTTTCTCTGTGTTTTGAAAGCGAACCACAGTACTACTGATTCAGGTACAAAAATTCGACGCCTTTCTGTTCAAGATGACAACATCACTATATCAGGGTCTGCATATGATTTGAATGGTATTCGTACCTTGCTAGTATTTGGCCAGGGGAGTTCTAATAATTTCCGCCAGTTTGGAAATGTACTTAAAAACTTCAAGTTTTTAAAGGTTTTAGATTTGCAAGGTGCGCCTTTGAAGAAGTTTCCAAAAGGTGTTGTCGGTCTCACCCTCTTAAGGTATTTGAGCCTAAGGGAAACTAAGATTAAAAGAGTTCCAAGGTCCATTATCAAGCTTGGATTCTTGGAAACCTTAGATCTTAAACACACCCAGATAACCCATTTGCCAAAAGAGATATGTAAACTCTCCAATCTGCGCCACCTCTTAGTCCATCACTGGGATGCTACATCTAAAAATATCCAAGCGGTAGTGGTTTCTTCAGGAAATATTAAAGCCCTACGGTCCATACAAAAGTTGTCACTAATTGAGGTGAAAAAGAGAAACAATAGAAAGCTCATAAAAGCCTTGGGAGAGTTGGTAGATCTAAGGAAGTTGGGGCTGCAAGTAGATCTTGCAAGTCAAGAAGATAGAAGACAATTATGTTCATCCATTCAGAAAATGAAACATCTTTCCATGCTTGATCTAAGATCAAAGAGCTTGGAAGTGTATCTAGATTTGGATCATATGGAATCCCCACCTAAGTATGTACAACGTCTACATTTGGAAGGGCTCCTAGACACGCTGCCGCGGTGGATCCCCAAGCTTGAAAGTCTGTCCAAGATAGCTCTAAAGTGCTCAAAACTGAATGATAGGGTGGAACCCCTTGAGGCCCTTGAGTCTTTGCCTAATCTGATGGAGCTCGACTTGGTTGATTATTCCGAAGGGGAAGTGTTGAAATTCAAAGCTGGAACGTTTAAAGAACTAATTCTAGTAAGTATAGAACAATTTGATCAGCTAAGAAAGATGGTCATCGAGAATGGGGCGATGCCTAAGCTTAAGAAGCTAACCATATCCAAATGTCGCAATCTGGAGTTAGTTCCAAAGGGCATCGACGGTCTCACATCCCTCGACCAACTGTGTGTAAATGACATGCACACGAAATTCATTGCTCACTTGAAAGGCATAGCGAAGAGAAGCAGCTGGGATATTTTTCGACGCAAGATTTCAGGAGGTTGCAAGATTGTTATTGATGGCACTGAGCAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

461

Amino Acids

52.35

Weight (kDa)

9.62

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 101 - 420 4.1e-48 Leucine-rich repeat region
LRR_8 PF13855 138 - 191 4.1e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 892
AccII CGCG 1 cut(s) 925
AciI CCGC 4 cut(s) 307, 602, 923, 925
AclI AACGTT 1 cut(s) 1088
AclWI GGATC 3 cut(s) 864, 925, 938
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 4 cut(s) 142, 195, 1073, 1277
AcuI CTGAAG 1 cut(s) 600
AcyI GRCGYC 1 cut(s) 202
AfaI GTAC 5 cut(s) 175, 190, 268, 324, 885
AfiI CCNNNNNNNGG 2 cut(s) 414, 574
AflII CTTAAG 2 cut(s) 412, 1172
AgsI TTSAA 9 cut(s) 160, 215, 256, 337, 376, 944, 1072, 1078, 1294
AjnI CCWGG 1 cut(s) 285
Alw21I GWGCWC 2 cut(s) 974, 1038
Alw26I GTCTC 2 cut(s) 407, 1238
AlwI GGATC 3 cut(s) 864, 925, 938
AlwNI CAGNNNCTG 1 cut(s) 242
AoxI GGCC 2 cut(s) 283, 1005
ApeKI GCWGC 4 cut(s) 63, 736, 919, 1314
ApoI RAATTY 4 cut(s) 142, 195, 1073, 1277
Asp700I GAANNNNTTC 1 cut(s) 1101
AspLEI GCGC 2 cut(s) 370, 555
AspS9I GGNCC 3 cut(s) 459, 639, 1006
AsuHPI GGTGA 3 cut(s) 107, 397, 679
AsuII TTCGAA 1 cut(s) 104
AvaII GGWCC 2 cut(s) 459, 639
AxyI CCTNAGG 1 cut(s) 428
BalI TGGCCA 1 cut(s) 285
BamHI GGATCC 1 cut(s) 930
BanII GRGCYC 2 cut(s) 909, 1038
BbsI GAAGAC 1 cut(s) 775
Bbv12I GWGCWC 2 cut(s) 974, 1038
BbvI GCAGC 4 cut(s) 75, 723, 906, 1326
BccI CCATC 5 cut(s) 71, 576, 1024, 1135, 1361
BciT130I CCWGG 1 cut(s) 287
BclI TGATCA 1 cut(s) 1126
BcoDI GTCTC 2 cut(s) 407, 1238
BfaI CTAG 4 cut(s) 275, 848, 911, 1106
BfrI CTTAAG 2 cut(s) 412, 1172
BglII AGATCT 3 cut(s) 493, 718, 745
BisI GCNGC 5 cut(s) 64, 737, 920, 923, 1315
BlsI GCNGC 5 cut(s) 65, 738, 921, 924, 1316
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 2 cut(s) 459, 639
BmgT120I GGNCC 3 cut(s) 459, 639, 1006
BmiI GGNNCC 3 cut(s) 908, 932, 996
BmrFI CCNGG 1 cut(s) 287
BmrI ACTGGG 1 cut(s) 583
BmsI GCATC 3 cut(s) 568, 1152, 1233
BmuI ACTGGG 1 cut(s) 583
BpiI GAAGAC 1 cut(s) 775
BpmI CTGGAG 1 cut(s) 1226
Bpu10I CCTNAGC 1 cut(s) 1167
Bpu14I TTCGAA 1 cut(s) 104
BpuEI CTTGAG 2 cut(s) 1022, 1031
BsaBI GATNNNNATC 2 cut(s) 855, 1145
BsaHI GRCGYC 1 cut(s) 202
BsaI GGTCTC 2 cut(s) 407, 1238
BsaJI CCNNGG 4 cut(s) 286, 455, 702, 923
BsaXI ACNNNNNCTCC 4 cut(s) 82, 112, 699, 729
Bsc4I CCNNNNNNNGG 2 cut(s) 414, 574
Bse1I ACTGG 3 cut(s) 39, 310, 578
Bse21I CCTNAGG 1 cut(s) 428
Bse3DI GCAATG 1 cut(s) 1281
Bse8I GATNNNNATC 2 cut(s) 855, 1145
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 4 cut(s) 286, 455, 702, 923
BseGI GGATG 3 cut(s) 583, 782, 1238
BseJI GATNNNNATC 2 cut(s) 855, 1145
BseLI CCNNNNNNNGG 2 cut(s) 414, 574
BseMI GCAATG 1 cut(s) 1281
BseMII CTCAG 2 cut(s) 129, 1365
BseNI ACTGG 3 cut(s) 39, 310, 578
BseXI GCAGC 4 cut(s) 75, 723, 906, 1326
BseYI CCCAGC 1 cut(s) 1317
Bsh1236I CGCG 1 cut(s) 925
BshFI GGCC 2 cut(s) 285, 1007
BsiHKAI GWGCWC 2 cut(s) 974, 1038
BslI CCNNNNNNNGG 2 cut(s) 414, 574
BsmAI GTCTC 2 cut(s) 407, 1238
BsnI GGCC 2 cut(s) 285, 1007
Bso31I GGTCTC 2 cut(s) 407, 1238
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 3 cut(s) 909, 974, 1038
Bsp1407I TGTACA 1 cut(s) 883
Bsp143I GATC 8 cut(s) 493, 718, 745, 817, 824, 856, 930, 1126
BspACI CCGC 4 cut(s) 307, 602, 923, 925
BspANI GGCC 2 cut(s) 285, 1007
BspCNI CTCAG 2 cut(s) 130, 1366
BspFNI CGCG 1 cut(s) 925
BspLI GGNNCC 3 cut(s) 908, 932, 996
BspPI GGATC 3 cut(s) 864, 925, 938
BspT104I TTCGAA 1 cut(s) 104
BspTI CTTAAG 2 cut(s) 412, 1172
BspTNI GGTCTC 2 cut(s) 407, 1238
BsrDI GCAATG 1 cut(s) 1281
BsrGI TGTACA 1 cut(s) 883
BsrI ACTGG 3 cut(s) 39, 310, 578
BssECI CCNNGG 4 cut(s) 286, 455, 702, 923
BssMI GATC 8 cut(s) 493, 718, 745, 817, 824, 856, 930, 1126
BssNI GRCGYC 1 cut(s) 202
BssT1I CCWWGG 2 cut(s) 455, 702
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 4 cut(s) 173, 639, 1232, 1254
Bst6I CTCTTC 1 cut(s) 1301
BstACI GRCGYC 1 cut(s) 202
BstAFI CTTAAG 2 cut(s) 412, 1172
BstAUI TGTACA 1 cut(s) 883
BstBI TTCGAA 1 cut(s) 104
BstDSI CCRYGG 1 cut(s) 923
BstF5I GGATG 3 cut(s) 583, 782, 1238
BstFNI CGCG 1 cut(s) 925
BstHHI GCGC 2 cut(s) 370, 555
BstKTI GATC 8 cut(s) 496, 721, 748, 820, 827, 859, 933, 1129
BstMAI GTCTC 2 cut(s) 407, 1238
BstMBI GATC 8 cut(s) 493, 718, 745, 817, 824, 856, 930, 1126
BstMWI GCNNNNNNNGC 3 cut(s) 63, 367, 1177
BstNI CCWGG 1 cut(s) 287
BstNSI RCATGY 1 cut(s) 1270
BstSCI CCNGG 1 cut(s) 285
BstUI CGCG 1 cut(s) 925
BstV1I GCAGC 4 cut(s) 75, 723, 906, 1326
BstV2I GAAGAC 1 cut(s) 775
BstX2I RGATCY 4 cut(s) 493, 718, 745, 930
BstYI RGATCY 4 cut(s) 493, 718, 745, 930
Bsu36I CCTNAGG 1 cut(s) 428
BsuRI GGCC 2 cut(s) 285, 1007
BtgI CCRYGG 1 cut(s) 923
BtgZI GCGATG 1 cut(s) 1175
BtsCI GGATG 3 cut(s) 583, 782, 1238
BtsIMutI CAGTG 2 cut(s) 571, 1371
CaiI CAGNNNCTG 1 cut(s) 242
CfoI GCGC 2 cut(s) 370, 555
Cfr13I GGNCC 3 cut(s) 459, 639, 1006
Cfr42I CCGCGG 1 cut(s) 926
CseI GACGC 3 cut(s) 46, 210, 1341
Csp6I GTAC 5 cut(s) 174, 189, 267, 323, 884
CviAII CATG 2 cut(s) 811, 1267
CviQI GTAC 5 cut(s) 174, 189, 267, 323, 884
DpnI GATC 8 cut(s) 495, 720, 747, 819, 826, 858, 932, 1128
DpnII GATC 8 cut(s) 493, 718, 745, 817, 824, 856, 930, 1126
DraI TTTAAA 2 cut(s) 345, 1093
EaeI YGGCCR 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 1301
EarI CTCTTC 1 cut(s) 1301
EciI GGCGGA 1 cut(s) 296
Ecl136II GAGCTC 1 cut(s) 1036
Eco130I CCWWGG 2 cut(s) 455, 702
Eco24I GRGCYC 2 cut(s) 909, 1038
Eco31I GGTCTC 2 cut(s) 407, 1238
Eco47I GGWCC 2 cut(s) 459, 639
Eco53kI GAGCTC 1 cut(s) 1036
Eco57I CTGAAG 1 cut(s) 600
Eco81I CCTNAGG 1 cut(s) 428
EcoICRI GAGCTC 1 cut(s) 1036
EcoO109I RGGNCCY 1 cut(s) 1006
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 2 cut(s) 455, 702
EcoT38I GRGCYC 2 cut(s) 909, 1038
ErhI CCWWGG 2 cut(s) 455, 702
FaeI CATG 2 cut(s) 814, 1270
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FatI CATG 2 cut(s) 810, 1266
FauNDI CATATG 2 cut(s) 247, 861
FbaI TGATCA 1 cut(s) 1126
FblI GTMKAC 1 cut(s) 892
Fnu4HI GCNGC 5 cut(s) 64, 737, 920, 923, 1315
FokI GGATG 3 cut(s) 590, 769, 1225
FriOI GRGCYC 2 cut(s) 909, 1038
Fsp4HI GCNGC 5 cut(s) 64, 737, 920, 923, 1315
FspBI CTAG 4 cut(s) 275, 848, 911, 1106
GlaI GCGC 2 cut(s) 369, 554
GluI GCNGC 5 cut(s) 64, 737, 920, 923, 1315
GsaI CCCAGC 1 cut(s) 1321
GsuI CTGGAG 1 cut(s) 1226
HaeIII GGCC 2 cut(s) 285, 1007
HgaI GACGC 3 cut(s) 46, 210, 1341
HhaI GCGC 2 cut(s) 370, 555
Hin1I GRCGYC 1 cut(s) 202
Hin1II CATG 2 cut(s) 814, 1270
Hin6I GCGC 2 cut(s) 368, 553
HinP1I GCGC 2 cut(s) 368, 553
HindIII AAGCTT 3 cut(s) 470, 938, 1169
HinfI GANTC 5 cut(s) 106, 182, 477, 866, 1013
HphI GGTGA 3 cut(s) 107, 397, 679
Hpy166II GTNNAC 3 cut(s) 115, 539, 893
Hpy188I TCNGA 3 cut(s) 792, 1029, 1057
Hpy188III TCNNGA 7 cut(s) 215, 618, 758, 848, 1150, 1205, 1344
Hpy8I GTNNAC 3 cut(s) 115, 539, 893
Hpy99I CGWCG 3 cut(s) 204, 1232, 1335
HpyAV CCTTC 2 cut(s) 896, 1052
HpyCH4III ACNGT 4 cut(s) 173, 639, 1232, 1254
HpyCH4IV ACGT 3 cut(s) 9, 889, 1088
HpyCH4V TGCA 7 cut(s) 14, 245, 361, 739, 752, 1270, 1353
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 367, 1177
HpySE526I ACGT 3 cut(s) 9, 889, 1088
Hsp92I GRCGYC 1 cut(s) 202
Hsp92II CATG 2 cut(s) 814, 1270
HspAI GCGC 2 cut(s) 368, 553
Ksp22I TGATCA 1 cut(s) 1126
KspI CCGCGG 1 cut(s) 926
Kzo9I GATC 8 cut(s) 493, 718, 745, 817, 824, 856, 930, 1126
LmnI GCTCC 2 cut(s) 912, 1033
Lsp1109I GCAGC 4 cut(s) 75, 723, 906, 1326
LweI GCATC 3 cut(s) 568, 1152, 1233
MaeI CTAG 4 cut(s) 275, 848, 911, 1106
MaeII ACGT 3 cut(s) 9, 889, 1088
MaeIII GTNAC 1 cut(s) 654
MalI GATC 8 cut(s) 495, 720, 747, 819, 826, 858, 932, 1128
MboI GATC 8 cut(s) 493, 718, 745, 817, 824, 856, 930, 1126
MboII GAAGA 5 cut(s) 388, 606, 773, 780, 1318
MflI RGATCY 4 cut(s) 493, 718, 745, 930
MhlI GDGCHC 3 cut(s) 909, 974, 1038
MlsI TGGCCA 1 cut(s) 285
MluCI AATT 9 cut(s) 142, 195, 301, 660, 773, 1073, 1101, 1121, 1277
MluNI TGGCCA 1 cut(s) 285
MlyI GAGTC 1 cut(s) 1022
MnlI CCTC 7 cut(s) 84, 419, 569, 658, 997, 1253, 1340
Mox20I TGGCCA 1 cut(s) 285
MroXI GAANNNNTTC 1 cut(s) 1101
MscI TGGCCA 1 cut(s) 285
MseI TTAA 8 cut(s) 327, 344, 413, 444, 498, 627, 1092, 1173
MslI CAYNNNNRTG 1 cut(s) 576
Msp20I TGGCCA 1 cut(s) 285
MspA1I CMGCKG 2 cut(s) 925, 1317
MspCI CTTAAG 2 cut(s) 412, 1172
MspR9I CCNGG 1 cut(s) 287
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 1 cut(s) 925
MwoI GCNNNNNNNGC 3 cut(s) 63, 367, 1177
NdeI CATATG 2 cut(s) 247, 861
NdeII GATC 8 cut(s) 493, 718, 745, 817, 824, 856, 930, 1126
NlaIII CATG 2 cut(s) 814, 1270
NlaIV GGNNCC 3 cut(s) 908, 932, 996
NmuCI GTSAC 1 cut(s) 654
NspI RCATGY 1 cut(s) 1270
NspV TTCGAA 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 1101
PfeI GAWTC 4 cut(s) 106, 182, 477, 866
PkrI GCNGC 5 cut(s) 65, 738, 921, 924, 1316
PleI GAGTC 1 cut(s) 1021
PpsI GAGTC 1 cut(s) 1021
Psp124BI GAGCTC 1 cut(s) 1038
Psp1406I AACGTT 1 cut(s) 1088
Psp6I CCWGG 1 cut(s) 285
PspFI CCCAGC 1 cut(s) 1317
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 3 cut(s) 908, 932, 996
PspPI GGNCC 3 cut(s) 459, 639, 1006
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 4 cut(s) 493, 718, 745, 930
PvuII CAGCTG 1 cut(s) 1317
RsaI GTAC 5 cut(s) 175, 190, 268, 324, 885
RsaNI GTAC 5 cut(s) 174, 189, 267, 323, 884
RseI CAYNNNNRTG 1 cut(s) 576
SacI GAGCTC 1 cut(s) 1038
SacII CCGCGG 1 cut(s) 926
SaqAI TTAA 8 cut(s) 327, 344, 413, 444, 498, 627, 1092, 1173
SatI GCNGC 5 cut(s) 64, 737, 920, 923, 1315
Sau3AI GATC 8 cut(s) 493, 718, 745, 817, 824, 856, 930, 1126
Sau96I GGNCC 3 cut(s) 459, 639, 1006
ScaI AGTACT 1 cut(s) 175
SchI GAGTC 1 cut(s) 1022
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 3 cut(s) 909, 974, 1038
SfaNI GCATC 3 cut(s) 568, 1152, 1233
Sfr303I CCGCGG 1 cut(s) 926
SfuI TTCGAA 1 cut(s) 104
SgrBI CCGCGG 1 cut(s) 926
SinI GGWCC 2 cut(s) 459, 639
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 4 cut(s) 412, 1001, 1010, 1172
SmoI CTYRAG 4 cut(s) 412, 1001, 1010, 1172
Sse9I AATT 9 cut(s) 142, 195, 301, 660, 773, 1073, 1101, 1121, 1277
SsiI CCGC 4 cut(s) 307, 602, 923, 925
SspI AATATT 1 cut(s) 625
SspMI CTAG 4 cut(s) 275, 848, 911, 1106
SstI GAGCTC 1 cut(s) 1038
StyD4I CCNGG 1 cut(s) 285
StyI CCWWGG 2 cut(s) 455, 702
TaaI ACNGT 4 cut(s) 173, 639, 1232, 1254
TaiI ACGT 3 cut(s) 12, 892, 1091
TaqI TCGA 7 cut(s) 104, 199, 1038, 1149, 1227, 1245, 1330
TaqII GACCGA 1 cut(s) 389
TasI AATT 9 cut(s) 142, 195, 301, 660, 773, 1073, 1101, 1121, 1277
TatI WGTACW 3 cut(s) 173, 322, 883
TauI GCSGC 1 cut(s) 925
TfiI GAWTC 4 cut(s) 106, 182, 477, 866
Tru1I TTAA 8 cut(s) 327, 344, 413, 444, 498, 627, 1092, 1173
Tru9I TTAA 8 cut(s) 327, 344, 413, 444, 498, 627, 1092, 1173
TscAI CASTG 2 cut(s) 578, 1378
TseFI GTSAC 1 cut(s) 654
TseI GCWGC 4 cut(s) 63, 736, 919, 1314
Tsp45I GTSAC 1 cut(s) 654
TspDTI ATGAA 5 cut(s) 45, 112, 771, 812, 1270
TspRI CASTG 2 cut(s) 578, 1378
Vha464I CTTAAG 2 cut(s) 412, 1172
VpaK11BI GGWCC 2 cut(s) 459, 639
XapI RAATTY 4 cut(s) 142, 195, 1073, 1277
XbaI TCTAGA 1 cut(s) 847
XceI RCATGY 1 cut(s) 1270
XmiI GTMKAC 1 cut(s) 892
XmnI GAANNNNTTC 1 cut(s) 1101
XspI CTAG 4 cut(s) 275, 848, 911, 1106
ZrmI AGTACT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.