Rh6AG099100

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
N/A
Physical Location & Seq
Forward (+)
14780493 .. 14798298
17806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG099100.1

Sequence Viewer

Length: 582 bp
ATGGGGGGTTGGACAAGGCTAGCGCGTTGGCCTGGACGAGGTGAGGCTCTAGATGAGGCTAGCGGCTGGAAGGCAGAGCGAGGGCAATCAGGAGGGAGCCAGGCTAACTTGCTGTCAAGGTTGATTGTAGACGAGGCGAGGCTCTCAGCGAGGCTAGCTGTTGGAAGGCAGAGTGAGGCTAATCAGGAGGGAGTGAGGCTAGCAGAGTTCGATCCCCCGCAATTTGTCTTGCAATTTGAAAACGAAGGTACCCCATCAGATATTAATTGTTTGCCGAGTTTACAGACTTCGGAATCTGAAATATCACCCTGGAAAGAAGATTGGAAAACTGTGTTGGGTGTATTAGACAGCTATAAAAGAGTTCCTAGCATAAAGATTGAAGATCTCAGAGCAACTTATAATGCAGTAGAAGATCCAGTGTTTGAAGAACTCATGAAAAAGCCTCCTTTGAATATTGAAGAAACTCGTATTTGGATGTATGACATGACTGACGAAATGGGTGAAGACCCTCAAAACTATAGAATGGAAAGTGTGTGTGCACGCGCGCGTGTGTATTTTCTGCTGGATATGTTGAAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

193

Amino Acids

21.97

Weight (kDa)

4.69

Isoelectric Point (pI)

59.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 399
Acc65I GGTACC 1 cut(s) 248
AccB1I GGYRCC 1 cut(s) 248
AccI GTMKAC 1 cut(s) 129
AccII CGCG 4 cut(s) 25, 543, 545, 547
AciI CCGC 2 cut(s) 63, 218
AclWI GGATC 2 cut(s) 206, 407
AfaI GTAC 1 cut(s) 250
AfiI CCNNNNNNNGG 1 cut(s) 38
AgsI TTSAA 6 cut(s) 239, 380, 425, 451, 458, 574
AjnI CCWGG 3 cut(s) 31, 99, 308
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 2 cut(s) 158, 351
AluI AGCT 2 cut(s) 158, 351
Alw21I GWGCWC 1 cut(s) 541
Alw44I GTGCAC 1 cut(s) 537
AlwI GGATC 2 cut(s) 206, 407
AoxI GGCC 1 cut(s) 29
ApaLI GTGCAC 1 cut(s) 537
AseI ATTAAT 1 cut(s) 264
Asp718I GGTACC 1 cut(s) 248
AspLEI GCGC 3 cut(s) 25, 545, 547
AsuHPI GGTGA 3 cut(s) 53, 297, 512
AsuNHI GCTAGC 4 cut(s) 19, 59, 154, 199
BaeGI GKGCMC 1 cut(s) 541
BaeI ACNNNNGTAYC 2 cut(s) 232, 265
BanI GGYRCC 1 cut(s) 248
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 541
BccI CCATC 1 cut(s) 262
BciT130I CCWGG 3 cut(s) 33, 101, 310
BfaI CTAG 6 cut(s) 20, 50, 60, 155, 200, 366
BfmI CTRYAG 1 cut(s) 517
BglII AGATCT 1 cut(s) 382
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme1390I CCNGG 3 cut(s) 33, 101, 310
BmiI GGNNCC 2 cut(s) 98, 250
BmrFI CCNGG 3 cut(s) 33, 101, 310
BmtI GCTAGC 4 cut(s) 23, 63, 158, 203
BpiI GAAGAC 1 cut(s) 510
BsaJI CCNNGG 1 cut(s) 308
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 416
BseBI CCWGG 3 cut(s) 33, 101, 310
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 38
BseMII CTCAG 2 cut(s) 159, 400
BseNI ACTGG 1 cut(s) 416
BsePI GCGCGC 1 cut(s) 543
BseSI GKGCMC 1 cut(s) 541
Bsh1236I CGCG 4 cut(s) 25, 543, 545, 547
BshFI GGCC 1 cut(s) 31
BshNI GGYRCC 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 541
BslI CCNNNNNNNGG 1 cut(s) 38
BsnI GGCC 1 cut(s) 31
Bsp1286I GDGCHC 1 cut(s) 541
Bsp143I GATC 3 cut(s) 211, 382, 412
BspACI CCGC 2 cut(s) 63, 218
BspANI GGCC 1 cut(s) 31
BspCNI CTCAG 2 cut(s) 158, 399
BspFNI CGCG 4 cut(s) 25, 543, 545, 547
BspHI TCATGA 1 cut(s) 432
BspLI GGNNCC 2 cut(s) 98, 250
BspOI GCTAGC 4 cut(s) 23, 63, 158, 203
BspPI GGATC 2 cut(s) 206, 407
BspT107I GGYRCC 1 cut(s) 248
BsrI ACTGG 1 cut(s) 416
BssECI CCNNGG 1 cut(s) 308
BssHII GCGCGC 1 cut(s) 543
BssMI GATC 3 cut(s) 211, 382, 412
Bst2UI CCWGG 3 cut(s) 33, 101, 310
Bst4CI ACNGT 1 cut(s) 331
BstC8I GCNNGC 6 cut(s) 21, 61, 156, 201, 541, 545
BstDEI CTNAG 2 cut(s) 145, 386
BstENI CCTNNNNNAGG 1 cut(s) 36
BstF5I GGATG 1 cut(s) 480
BstFNI CGCG 4 cut(s) 25, 543, 545, 547
BstHHI GCGC 3 cut(s) 25, 545, 547
BstKTI GATC 3 cut(s) 214, 385, 415
BstMBI GATC 3 cut(s) 211, 382, 412
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 3 cut(s) 33, 101, 310
BstSCI CCNGG 3 cut(s) 31, 99, 308
BstSFI CTRYAG 1 cut(s) 517
BstSLI GKGCMC 1 cut(s) 541
BstUI CGCG 4 cut(s) 25, 543, 545, 547
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 2 cut(s) 382, 412
BstYI RGATCY 2 cut(s) 382, 412
BsuRI GGCC 1 cut(s) 31
BtsCI GGATG 1 cut(s) 480
BtsIMutI CAGTG 1 cut(s) 423
Cac8I GCNNGC 6 cut(s) 21, 61, 156, 201, 541, 545
CciI TCATGA 1 cut(s) 432
CfoI GCGC 3 cut(s) 25, 545, 547
Csp6I GTAC 1 cut(s) 249
CviAII CATG 2 cut(s) 433, 484
CviQI GTAC 1 cut(s) 249
DdeI CTNAG 2 cut(s) 145, 386
DpnI GATC 3 cut(s) 213, 384, 414
DpnII GATC 3 cut(s) 211, 382, 412
EcoNI CCTNNNNNAGG 1 cut(s) 36
EcoRII CCWGG 3 cut(s) 31, 99, 308
FaeI CATG 2 cut(s) 436, 487
FaiI YATR 8 cut(s) 354, 371, 399, 434, 480, 485, 519, 569
FatI CATG 2 cut(s) 432, 483
FauI CCCGC 1 cut(s) 225
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 1 cut(s) 487
Fsp4HI GCNGC 1 cut(s) 64
FspBI CTAG 6 cut(s) 20, 50, 60, 155, 200, 366
GlaI GCGC 3 cut(s) 24, 544, 546
GluI GCNGC 1 cut(s) 64
HaeIII GGCC 1 cut(s) 31
HhaI GCGC 3 cut(s) 25, 545, 547
Hin1II CATG 2 cut(s) 436, 487
Hin6I GCGC 3 cut(s) 23, 543, 545
HinP1I GCGC 3 cut(s) 23, 543, 545
HinfI GANTC 1 cut(s) 293
HphI GGTGA 3 cut(s) 53, 297, 512
Hpy166II GTNNAC 3 cut(s) 130, 281, 539
Hpy188I TCNGA 4 cut(s) 259, 292, 298, 389
Hpy188III TCNNGA 4 cut(s) 50, 90, 185, 433
Hpy8I GTNNAC 3 cut(s) 130, 281, 539
HpyAV CCTTC 3 cut(s) 64, 159, 239
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4V TGCA 3 cut(s) 232, 404, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 2 cut(s) 145, 386
Hsp92II CATG 2 cut(s) 436, 487
HspAI GCGC 3 cut(s) 23, 543, 545
KpnI GGTACC 1 cut(s) 252
Kzo9I GATC 3 cut(s) 211, 382, 412
LmnI GCTCC 1 cut(s) 96
MaeI CTAG 6 cut(s) 20, 50, 60, 155, 200, 366
MalI GATC 3 cut(s) 213, 384, 414
MauBI CGCGCGCG 1 cut(s) 543
MboI GATC 3 cut(s) 211, 382, 412
MboII GAAGA 6 cut(s) 329, 392, 422, 437, 470, 515
MflI RGATCY 2 cut(s) 382, 412
MhlI GDGCHC 1 cut(s) 541
MluCI AATT 3 cut(s) 221, 233, 265
MmeI TCCRAC 1 cut(s) 142
MseI TTAA 2 cut(s) 264, 580
MspR9I CCNGG 3 cut(s) 33, 101, 310
MvaI CCWGG 3 cut(s) 33, 101, 310
MvnI CGCG 4 cut(s) 25, 543, 545, 547
MwoI GCNNNNNNNGC 1 cut(s) 155
NdeII GATC 3 cut(s) 211, 382, 412
NheI GCTAGC 4 cut(s) 19, 59, 154, 199
NlaIII CATG 2 cut(s) 436, 487
NlaIV GGNNCC 2 cut(s) 98, 250
NmeAIII GCCGAG 1 cut(s) 300
PagI TCATGA 1 cut(s) 432
PauI GCGCGC 1 cut(s) 543
PfeI GAWTC 1 cut(s) 293
PkrI GCNGC 1 cut(s) 65
PshBI ATTAAT 1 cut(s) 264
PsiI TTATAA 1 cut(s) 399
Psp6I CCWGG 3 cut(s) 31, 99, 308
PspGI CCWGG 3 cut(s) 31, 99, 308
PspN4I GGNNCC 2 cut(s) 98, 250
PsuI RGATCY 2 cut(s) 382, 412
PteI GCGCGC 1 cut(s) 543
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
SaqAI TTAA 2 cut(s) 264, 580
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 3 cut(s) 211, 382, 412
ScrFI CCNGG 3 cut(s) 33, 101, 310
SduI GDGCHC 1 cut(s) 541
SetI ASST 5 cut(s) 43, 122, 160, 250, 353
SfcI CTRYAG 1 cut(s) 517
Sse9I AATT 3 cut(s) 221, 233, 265
SsiI CCGC 2 cut(s) 63, 218
SspI AATATT 1 cut(s) 454
SspMI CTAG 6 cut(s) 20, 50, 60, 155, 200, 366
StyD4I CCNGG 3 cut(s) 31, 99, 308
TaaI ACNGT 1 cut(s) 331
TaqI TCGA 1 cut(s) 210
TasI AATT 3 cut(s) 221, 233, 265
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 1 cut(s) 293
Tru1I TTAA 2 cut(s) 264, 580
Tru9I TTAA 2 cut(s) 264, 580
TscAI CASTG 1 cut(s) 423
TspDTI ATGAA 1 cut(s) 449
TspRI CASTG 1 cut(s) 423
VneI GTGCAC 1 cut(s) 537
VspI ATTAAT 1 cut(s) 264
XagI CCTNNNNNAGG 1 cut(s) 36
XbaI TCTAGA 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 6 cut(s) 20, 50, 60, 155, 200, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.