Rh6BG022400
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
N/A
Physical Location & Seq
Forward (+)
3282009 .. 3286573
4565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG022400.1

Sequence Viewer

Length: 1059 bp
ATGGCATTAGCGGCGAAAGGCGAGGGTCCAGCTATCGGCATTGATCTCGGGACAACTTACTCATGCGTTGGCGTTTGGCAACACGATCGCGTTGAGATCATAGCCAATGATCAGGGCAACAGAACGACACCATCGAAATTTAGTGATGCATGTGTCAAGAGTGATATGAAACTATGGCCTTTCAAGGTTCGTGGTGTGGGTAACAAGCCTATGATTGTGATCAACTATAAGAACGAAAAGAAACAATTTTCCGCTGAGGAGATATCTTCAATGGTTCTCACCGCAGCTATTGCATATGGTCTTGACCAAAAGTCCACGATTATTGGTGGTAAAAATGTCCTCATTTTCGATCTTGGTGGTGGTACTTCTGATGTTTCCCTTCTTAAAATTGAAGATGAGATTTTTGAGGTAAAAGCTACTGCTGGAGATTCACATCTTGGGGGTGAAGATTTTGACAATAGAATGGTGAACCATTTTGTGCAAGAGTTCGAGAAGAAACACAGGAAGGACATCAGCAATGACCCCAAAGCACTCAGAAGATTGAGAACATCTTGTGAGAGAGCGAAAAGGACTCTTTCTTCAACTGCTGAAACCACCATTGAGGTTGATGCTTTATTCGAGGGTATAGACTTCCACTCTAAAATAACAAGAGCAAAATTTGAAGAGCTCAACATGGATCTTTTTAGAAAATGTATGGAGACTGTGGAGAATTGTGTGAGGGATGCTAAACTTGATAGGAAAATTGTTGATGATATTGTTCTTGTTGGTGGATCTACTAGAATTCCCAAGGTACAACATCTATTGCAAGATTTCTTTAATGGTAAGGAGCTTTGCAAGAGCATCAATCCTGATGAAGCAGTTGCATACGGTGCTGCCGTACAGGCTACAGTTTTGCGTGGTGGCATTGAGAAGGTTCAAGACTTACTCTTGTTGGATGTAACTCCACTTTCCCTTGGTTGTATGCGTCACACAGGAGCATTTGAGGTTTTTATCCCAAGAAACACACCCATTCCCGCCAAAAAAAGTAAAATGGCCACAACTGTCGTGCAAAGCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

352

Amino Acids

38.88

Weight (kDa)

7.04

Isoelectric Point (pI)

28.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 11 - 45 2.2e-12 Hsp70 protein
MreB_Mbl PF06723 91 - 293 1.2e-07 MreB/Mbl protein
HSP70 PF00012 95 - 352 6.9e-99 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 90
AciI CCGC 4 cut(s) 11, 252, 282, 1014
AclWI GGATC 2 cut(s) 684, 778
AcoI YGGCCR 1 cut(s) 1032
AcsI RAATTY 3 cut(s) 137, 656, 780
AfaI GTAC 3 cut(s) 364, 792, 879
AfiI CCNNNNNNNGG 1 cut(s) 35
AgsI TTSAA 6 cut(s) 184, 270, 392, 582, 662, 917
AhdI GACNNNNNGTC 1 cut(s) 310
AluBI AGCT 5 cut(s) 32, 287, 416, 667, 829
AluI AGCT 5 cut(s) 32, 287, 416, 667, 829
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 692
AlwI GGATC 2 cut(s) 684, 778
Ama87I CYCGRG 1 cut(s) 47
AoxI GGCC 2 cut(s) 176, 1032
ApeKI GCWGC 2 cut(s) 284, 872
ApoI RAATTY 3 cut(s) 137, 656, 780
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 3 cut(s) 271, 455, 478
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 1 cut(s) 26
BalI TGGCCA 1 cut(s) 1034
BanII GRGCYC 1 cut(s) 669
Bbv12I GWGCWC 1 cut(s) 669
BbvCI CCTCAGC 1 cut(s) 255
BbvI GCAGC 2 cut(s) 296, 859
BccI CCATC 1 cut(s) 139
BceAI ACGGC 1 cut(s) 860
BcgI CGANNNNNNTGC 4 cut(s) 28, 62, 68, 102
BclI TGATCA 2 cut(s) 109, 219
BcoDI GTCTC 1 cut(s) 692
BfaI CTAG 1 cut(s) 777
BfmI CTRYAG 1 cut(s) 885
BglI GCCNNNNNGGC 1 cut(s) 881
BisI GCNGC 3 cut(s) 12, 285, 873
BlsI GCNGC 3 cut(s) 13, 286, 874
Bme18I GGWCC 1 cut(s) 26
BmeRI GACNNNNNGTC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 27
BmsI GCATC 4 cut(s) 136, 598, 712, 849
BpmI CTGGAG 1 cut(s) 444
Bpu10I CCTNAGC 1 cut(s) 255
BsaBI GATNNNNATC 2 cut(s) 218, 432
BsaJI CCNNGG 2 cut(s) 786, 952
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse3DI GCAATG 1 cut(s) 523
Bse8I GATNNNNATC 2 cut(s) 218, 432
BseDI CCNNGG 2 cut(s) 786, 952
BseGI GGATG 2 cut(s) 727, 940
BseJI GATNNNNATC 2 cut(s) 218, 432
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMI GCAATG 1 cut(s) 523
BseMII CTCAG 2 cut(s) 246, 547
BseRI GAGGAG 1 cut(s) 272
BseXI GCAGC 2 cut(s) 296, 859
Bsh1236I CGCG 1 cut(s) 90
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 2 cut(s) 178, 1034
BsiEI CGRYCG 1 cut(s) 88
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 1 cut(s) 47
BslFI GGGAC 1 cut(s) 64
BslI CCNNNNNNNGG 1 cut(s) 35
BsmAI GTCTC 1 cut(s) 692
BsmFI GGGAC 1 cut(s) 64
BsnI GGCC 2 cut(s) 178, 1034
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 8 cut(s) 43, 85, 96, 109, 219, 349, 676, 770
BspACI CCGC 4 cut(s) 11, 252, 282, 1014
BspANI GGCC 2 cut(s) 178, 1034
BspCNI CTCAG 2 cut(s) 247, 546
BspFNI CGCG 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 27
BspPI GGATC 2 cut(s) 684, 778
BspQI GCTCTTC 1 cut(s) 657
BsrDI GCAATG 1 cut(s) 523
BssECI CCNNGG 2 cut(s) 786, 952
BssMI GATC 8 cut(s) 43, 85, 96, 109, 219, 349, 676, 770
BssT1I CCWWGG 2 cut(s) 786, 952
Bst4CI ACNGT 4 cut(s) 703, 869, 889, 1042
Bst6I CTCTTC 1 cut(s) 657
BstAPI GCANNNNNTGC 2 cut(s) 290, 869
BstDEI CTNAG 2 cut(s) 255, 533
BstF5I GGATG 2 cut(s) 727, 940
BstFNI CGCG 1 cut(s) 90
BstKTI GATC 8 cut(s) 46, 88, 99, 112, 222, 352, 679, 773
BstMAI GTCTC 1 cut(s) 692
BstMBI GATC 8 cut(s) 43, 85, 96, 109, 219, 349, 676, 770
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 4 cut(s) 11, 290, 869, 881
BstNSI RCATGY 1 cut(s) 153
BstSFI CTRYAG 1 cut(s) 885
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 2 cut(s) 296, 859
BstX2I RGATCY 2 cut(s) 676, 770
BstYI RGATCY 2 cut(s) 676, 770
BsuRI GGCC 2 cut(s) 178, 1034
BtsCI GGATG 2 cut(s) 727, 940
Cfr13I GGNCC 1 cut(s) 26
CseI GACGC 1 cut(s) 953
Csp6I GTAC 3 cut(s) 363, 791, 878
CspCI CAANNNNNGTGG 4 cut(s) 172, 207, 304, 339
CviAII CATG 3 cut(s) 63, 150, 673
CviQI GTAC 3 cut(s) 363, 791, 878
DdeI CTNAG 2 cut(s) 255, 533
DpnI GATC 8 cut(s) 45, 87, 98, 111, 221, 351, 678, 772
DpnII GATC 8 cut(s) 43, 85, 96, 109, 219, 349, 676, 770
DriI GACNNNNNGTC 1 cut(s) 310
EaeI YGGCCR 1 cut(s) 1032
Eam1104I CTCTTC 1 cut(s) 657
Eam1105I GACNNNNNGTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 657
Ecl136II GAGCTC 1 cut(s) 667
Eco130I CCWWGG 2 cut(s) 786, 952
Eco24I GRGCYC 1 cut(s) 669
Eco32I GATATC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 667
Eco88I CYCGRG 1 cut(s) 47
EcoICRI GAGCTC 1 cut(s) 667
EcoRI GAATTC 1 cut(s) 780
EcoRV GATATC 1 cut(s) 264
EcoT14I CCWWGG 2 cut(s) 786, 952
EcoT22I ATGCAT 1 cut(s) 151
EcoT38I GRGCYC 1 cut(s) 669
ErhI CCWWGG 2 cut(s) 786, 952
FaeI CATG 3 cut(s) 66, 153, 676
FaqI GGGAC 1 cut(s) 64
FatI CATG 3 cut(s) 62, 149, 672
FauI CCCGC 1 cut(s) 1021
FauNDI CATATG 1 cut(s) 295
FbaI TGATCA 2 cut(s) 109, 219
Fnu4HI GCNGC 3 cut(s) 12, 285, 873
FokI GGATG 2 cut(s) 734, 947
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 3 cut(s) 12, 285, 873
FspBI CTAG 1 cut(s) 777
GluI GCNGC 3 cut(s) 12, 285, 873
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 2 cut(s) 178, 1034
HgaI GACGC 1 cut(s) 953
Hin1II CATG 3 cut(s) 66, 153, 676
HinfI GANTC 2 cut(s) 428, 571
HphI GGTGA 3 cut(s) 271, 455, 478
Hpy166II GTNNAC 2 cut(s) 315, 469
Hpy188I TCNGA 2 cut(s) 370, 536
Hpy188III TCNNGA 6 cut(s) 49, 157, 302, 490, 848, 917
Hpy8I GTNNAC 2 cut(s) 315, 469
HpyAV CCTTC 3 cut(s) 389, 499, 904
HpyCH4III ACNGT 4 cut(s) 703, 869, 889, 1042
HpyCH4V TGCA 7 cut(s) 149, 293, 481, 805, 834, 863, 1048
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 290, 869, 881
HpyF3I CTNAG 2 cut(s) 255, 533
Hsp92II CATG 3 cut(s) 66, 153, 676
Ksp22I TGATCA 2 cut(s) 109, 219
Kzo9I GATC 8 cut(s) 43, 85, 96, 109, 219, 349, 676, 770
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 2 cut(s) 826, 974
LpnPI CCDG 7 cut(s) 42, 98, 408, 487, 861, 866, 957
Lsp1109I GCAGC 2 cut(s) 296, 859
LweI GCATC 4 cut(s) 136, 598, 712, 849
MaeI CTAG 1 cut(s) 777
MaeIII GTNAC 3 cut(s) 200, 937, 965
MalI GATC 8 cut(s) 45, 87, 98, 111, 221, 351, 678, 772
MboI GATC 8 cut(s) 43, 85, 96, 109, 219, 349, 676, 770
MboII GAAGA 7 cut(s) 258, 404, 458, 505, 549, 570, 674
MflI RGATCY 2 cut(s) 676, 770
MhlI GDGCHC 1 cut(s) 669
MlsI TGGCCA 1 cut(s) 1034
MluCI AATT 7 cut(s) 137, 245, 387, 656, 709, 741, 780
MluNI TGGCCA 1 cut(s) 1034
MlyI GAGTC 1 cut(s) 565
MmeI TCCRAC 1 cut(s) 912
MnlI CCTC 8 cut(s) 16, 250, 350, 400, 595, 613, 711, 976
Mox20I TGGCCA 1 cut(s) 1034
Mph1103I ATGCAT 1 cut(s) 151
MscI TGGCCA 1 cut(s) 1034
MseI TTAA 2 cut(s) 384, 816
Msp20I TGGCCA 1 cut(s) 1034
MspA1I CMGCKG 1 cut(s) 254
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 4 cut(s) 11, 290, 869, 881
NdeI CATATG 1 cut(s) 295
NdeII GATC 8 cut(s) 43, 85, 96, 109, 219, 349, 676, 770
NlaIII CATG 3 cut(s) 66, 153, 676
NlaIV GGNNCC 1 cut(s) 27
NmuCI GTSAC 1 cut(s) 965
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 1 cut(s) 153
PciSI GCTCTTC 1 cut(s) 657
PcsI WCGNNNNNNNCGW 2 cut(s) 131, 873
PfeI GAWTC 1 cut(s) 428
PkrI GCNGC 3 cut(s) 13, 286, 874
Ple19I CGATCG 1 cut(s) 88
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Psp124BI GAGCTC 1 cut(s) 669
PspN4I GGNNCC 1 cut(s) 27
PspPI GGNCC 1 cut(s) 26
PsuI RGATCY 2 cut(s) 676, 770
PvuI CGATCG 1 cut(s) 88
RsaI GTAC 3 cut(s) 364, 792, 879
RsaNI GTAC 3 cut(s) 363, 791, 878
SacI GAGCTC 1 cut(s) 669
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 2 cut(s) 384, 816
SatI GCNGC 3 cut(s) 12, 285, 873
Sau3AI GATC 8 cut(s) 43, 85, 96, 109, 219, 349, 676, 770
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 1 cut(s) 565
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 4 cut(s) 136, 598, 712, 849
SfcI CTRYAG 1 cut(s) 885
SinI GGWCC 1 cut(s) 26
Sse9I AATT 7 cut(s) 137, 245, 387, 656, 709, 741, 780
SsiI CCGC 4 cut(s) 11, 252, 282, 1014
SspMI CTAG 1 cut(s) 777
SstI GAGCTC 1 cut(s) 669
StyI CCWWGG 2 cut(s) 786, 952
TaaI ACNGT 4 cut(s) 703, 869, 889, 1042
TaqI TCGA 4 cut(s) 134, 348, 489, 618
TasI AATT 7 cut(s) 137, 245, 387, 656, 709, 741, 780
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 2 cut(s) 384, 816
Tru9I TTAA 2 cut(s) 384, 816
TseFI GTSAC 1 cut(s) 965
TseI GCWGC 2 cut(s) 284, 872
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 2 cut(s) 182, 867
VpaK11BI GGWCC 1 cut(s) 26
XapI RAATTY 3 cut(s) 137, 656, 780
XceI RCATGY 1 cut(s) 153
XspI CTAG 1 cut(s) 777
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.