Rw2G020650

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
N/A
Physical Location & Seq
Forward (+)
28184036 .. 28185306
1271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020650.1

Sequence Viewer

Length: 912 bp
ATGAAGCTCTTCAGCTTTTTTTACCGGAAGGCCTTTAAAAAAGATTACCCTGAACGAAGCTACCTGGATTTGTCTAATTATGTTGTGAATTATGCCAAAGGTCTTCCTCTGTCTCTTAAAGTCTTGGGTTCCTTTTTGCATGGAAGAGGTCGAAGTGCGTTGGCAAAATTGAGAAAAGTTTGTAATGCAGAAATTTTCGAGAAACTTAAAATAAGTTATGATGGTCAAGACGACAATGAGAAGAAAATCTTCCTAGACATTGCATGTTTCTACAGAGGGTACGAGAAAAAAAAAGCAATAGAAAGACTTGACTTGTGTGGCTTTTATGCAACTATTGCAATTGATGTACTTGTGGAGAGATCTCTCTTAACTATTTCAGATGCTGGCACTCTACAAATGCATGATTTGCTCCAAGAAATGGGCCTAGAAATTGTTCGCCGAGAATCTCCTGACGAGCCAGGCAAGCGCAGCAGGTTAATACTGATACTTTATGGTGTTTATATGCACCTCTCTGACGACTTTCTGCATCTTTCAAGTGAGTTACGGTTACTCGTATGGTGGAATTGTCCTCTACAATCATTGGCATCACTTATCAACCTGGAGAACTTACAAGAACTTGAAATGTGCCATAGCCAAATTGAATATCTGTGGGAGAGTAAACAGCCTTTATACAGTTTGAAACGCATCGACCTCAGCCACTCTTTGAATCTTCTCAAGACCCCCGACTTTAGCGGTAGTTGTATACAGCTGTACGAGGTTGACTCATCCGTTGGATTCCTTGAAAGACTTACTGAGATGTTCTTGAGTGGTTGCAAAAACCTTAGAGCAAGTGCACCCGTAGTCAAGAAAAGGCAAAGTCGAGAAGTCAAGAATTCGACCAGTCAAGTTACTATTCACTGCCACTGGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

303

Amino Acids

35.05

Weight (kDa)

8.5

Isoelectric Point (pI)

57.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 78 - 149 1e-20 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 462
AccB7I CCANNNNNTGG 1 cut(s) 418
AccI GTMKAC 1 cut(s) 742
AciI CCGC 1 cut(s) 732
AcsI RAATTY 2 cut(s) 192, 871
AfaI GTAC 3 cut(s) 281, 348, 752
AfiI CCNNNNNNNGG 1 cut(s) 418
AgsI TTSAA 6 cut(s) 534, 620, 641, 679, 706, 782
AjnI CCWGG 3 cut(s) 63, 457, 597
AluBI AGCT 4 cut(s) 7, 15, 60, 748
AluI AGCT 4 cut(s) 7, 15, 60, 748
Alw21I GWGCWC 1 cut(s) 835
Alw26I GTCTC 1 cut(s) 117
Alw44I GTGCAC 1 cut(s) 831
AlwNI CAGNNNCTG 1 cut(s) 383
AoxI GGCC 2 cut(s) 30, 421
ApaLI GTGCAC 1 cut(s) 831
ApeKI GCWGC 1 cut(s) 468
ApoI RAATTY 2 cut(s) 192, 871
Asp700I GAANNNNTTC 3 cut(s) 8, 248, 432
AspLEI GCGC 1 cut(s) 468
AspS9I GGNCC 1 cut(s) 421
BaeGI GKGCMC 1 cut(s) 835
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 835
BbvCI CCTCAGC 1 cut(s) 692
BbvI GCAGC 1 cut(s) 480
BccI CCATC 1 cut(s) 215
BciT130I CCWGG 3 cut(s) 65, 459, 599
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 2 cut(s) 254, 425
BfmI CTRYAG 1 cut(s) 271
BfuAI ACCTGC 1 cut(s) 462
BglII AGATCT 1 cut(s) 359
BisI GCNGC 1 cut(s) 469
BlsI GCNGC 1 cut(s) 470
Bme1390I CCNGG 3 cut(s) 65, 459, 599
BmgT120I GGNCC 1 cut(s) 421
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 3 cut(s) 65, 459, 599
BmsI GCATC 4 cut(s) 370, 535, 593, 693
BpiI GAAGAC 1 cut(s) 95
BplI GAGNNNNNCTC 2 cut(s) 746, 778
BpmI CTGGAG 1 cut(s) 620
Bpu10I CCTNAGC 1 cut(s) 692
BpuEI CTTGAG 2 cut(s) 698, 823
BsaWI WCCGGW 1 cut(s) 24
Bsc4I CCNNNNNNNGG 1 cut(s) 418
Bse1I ACTGG 2 cut(s) 879, 908
Bse3DI GCAATG 1 cut(s) 258
BseBI CCWGG 3 cut(s) 65, 459, 599
BseGI GGATG 1 cut(s) 764
BseLI CCNNNNNNNGG 1 cut(s) 418
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 706, 783
BseNI ACTGG 2 cut(s) 879, 908
BseSI GKGCMC 1 cut(s) 835
BseXI GCAGC 1 cut(s) 480
BshFI GGCC 2 cut(s) 32, 423
BsiHKAI GWGCWC 1 cut(s) 835
BsiSI CCGG 1 cut(s) 25
BslI CCNNNNNNNGG 1 cut(s) 418
BsmAI GTCTC 1 cut(s) 117
BsnI GGCC 2 cut(s) 32, 423
Bsp1286I GDGCHC 1 cut(s) 835
Bsp143I GATC 1 cut(s) 359
BspACI CCGC 1 cut(s) 732
BspANI GGCC 2 cut(s) 32, 423
BspCNI CTCAG 2 cut(s) 705, 784
BspLI GGNNCC 1 cut(s) 130
BspMI ACCTGC 1 cut(s) 462
BspQI GCTCTTC 1 cut(s) 14
BsrDI GCAATG 1 cut(s) 258
BsrI ACTGG 2 cut(s) 879, 908
BssMI GATC 1 cut(s) 359
BssNAI GTATAC 1 cut(s) 743
Bst1107I GTATAC 1 cut(s) 743
Bst2UI CCWGG 3 cut(s) 65, 459, 599
Bst4CI ACNGT 2 cut(s) 546, 674
Bst6I CTCTTC 2 cut(s) 14, 139
BstAPI GCANNNNNTGC 2 cut(s) 335, 406
BstC8I GCNNGC 2 cut(s) 385, 464
BstDEI CTNAG 3 cut(s) 692, 792, 821
BstF5I GGATG 1 cut(s) 764
BstHHI GCGC 1 cut(s) 468
BstKTI GATC 1 cut(s) 362
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 1 cut(s) 359
BstMWI GCNNNNNNNGC 4 cut(s) 335, 406, 463, 468
BstNI CCWGG 3 cut(s) 65, 459, 599
BstNSI RCATGY 1 cut(s) 267
BstSCI CCNGG 3 cut(s) 63, 457, 597
BstSFI CTRYAG 1 cut(s) 271
BstSLI GKGCMC 1 cut(s) 835
BstV1I GCAGC 1 cut(s) 480
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 359
BstYI RGATCY 1 cut(s) 359
BstZ17I GTATAC 1 cut(s) 743
BsuRI GGCC 2 cut(s) 32, 423
BtsCI GGATG 1 cut(s) 764
BtsI GCAGTG 1 cut(s) 895
BtsIMutI CAGTG 2 cut(s) 895, 901
BveI ACCTGC 1 cut(s) 462
Cac8I GCNNGC 2 cut(s) 385, 464
CaiI CAGNNNCTG 1 cut(s) 383
CfoI GCGC 1 cut(s) 468
Cfr13I GGNCC 1 cut(s) 421
Csp6I GTAC 3 cut(s) 280, 347, 751
CviAII CATG 3 cut(s) 140, 264, 401
CviQI GTAC 3 cut(s) 280, 347, 751
DdeI CTNAG 3 cut(s) 692, 792, 821
DpnI GATC 1 cut(s) 361
DpnII GATC 1 cut(s) 359
DraI TTTAAA 1 cut(s) 37
Eam1104I CTCTTC 2 cut(s) 14, 139
EarI CTCTTC 2 cut(s) 14, 139
Eco147I AGGCCT 1 cut(s) 32
EcoRI GAATTC 1 cut(s) 871
EcoRII CCWGG 3 cut(s) 63, 457, 597
EcoT22I ATGCAT 1 cut(s) 402
FaeI CATG 3 cut(s) 143, 267, 404
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FatI CATG 3 cut(s) 139, 263, 400
FblI GTMKAC 1 cut(s) 742
Fnu4HI GCNGC 1 cut(s) 469
FokI GGATG 1 cut(s) 751
Fsp4HI GCNGC 1 cut(s) 469
FspBI CTAG 2 cut(s) 254, 425
GlaI GCGC 1 cut(s) 467
GluI GCNGC 1 cut(s) 469
GsuI CTGGAG 1 cut(s) 620
HaeIII GGCC 2 cut(s) 32, 423
HapII CCGG 1 cut(s) 25
HhaI GCGC 1 cut(s) 468
Hin1II CATG 3 cut(s) 143, 267, 404
Hin6I GCGC 1 cut(s) 466
HinP1I GCGC 1 cut(s) 466
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HinfI GANTC 4 cut(s) 443, 706, 761, 774
HpaII CCGG 1 cut(s) 25
Hpy166II GTNNAC 4 cut(s) 659, 743, 760, 833
Hpy188I TCNGA 2 cut(s) 379, 514
Hpy188III TCNNGA 8 cut(s) 199, 227, 449, 715, 802, 844, 860, 868
Hpy8I GTNNAC 4 cut(s) 659, 743, 760, 833
HpyAV CCTTC 1 cut(s) 22
HpyCH4III ACNGT 2 cut(s) 546, 674
HpyF10VI GCNNNNNNNGC 4 cut(s) 335, 406, 463, 468
HpyF3I CTNAG 3 cut(s) 692, 792, 821
Hsp92II CATG 3 cut(s) 143, 267, 404
HspAI GCGC 1 cut(s) 466
Kzo9I GATC 1 cut(s) 359
LguI GCTCTTC 1 cut(s) 14
LmnI GCTCC 1 cut(s) 414
Lsp1109I GCAGC 1 cut(s) 480
LweI GCATC 4 cut(s) 370, 535, 593, 693
MaeI CTAG 2 cut(s) 254, 425
MaeIII GTNAC 3 cut(s) 540, 546, 886
MalI GATC 1 cut(s) 361
MboI GATC 1 cut(s) 359
MboII GAAGA 5 cut(s) 95, 156, 241, 253, 701
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 1 cut(s) 359
MhlI GDGCHC 1 cut(s) 835
MluCI AATT 9 cut(s) 76, 88, 167, 192, 339, 429, 562, 636, 871
MlyI GAGTC 1 cut(s) 755
MmeI TCCRAC 1 cut(s) 751
MnlI CCTC 7 cut(s) 117, 140, 269, 518, 579, 701, 748
Mph1103I ATGCAT 1 cut(s) 402
MroXI GAANNNNTTC 3 cut(s) 8, 248, 432
MseI TTAA 5 cut(s) 36, 117, 207, 368, 476
MspA1I CMGCKG 1 cut(s) 748
MspI CCGG 1 cut(s) 25
MspR9I CCNGG 3 cut(s) 65, 459, 599
MunI CAATTG 1 cut(s) 339
MvaI CCWGG 3 cut(s) 65, 459, 599
MwoI GCNNNNNNNGC 4 cut(s) 335, 406, 463, 468
NdeII GATC 1 cut(s) 359
NlaIII CATG 3 cut(s) 143, 267, 404
NlaIV GGNNCC 1 cut(s) 130
NmeAIII GCCGAG 1 cut(s) 464
NsiI ATGCAT 1 cut(s) 402
NspI RCATGY 1 cut(s) 267
PceI AGGCCT 1 cut(s) 32
PciSI GCTCTTC 1 cut(s) 14
PdmI GAANNNNTTC 3 cut(s) 8, 248, 432
PfeI GAWTC 3 cut(s) 443, 706, 774
PflMI CCANNNNNTGG 1 cut(s) 418
PkrI GCNGC 1 cut(s) 470
PleI GAGTC 1 cut(s) 755
PpsI GAGTC 1 cut(s) 755
Psp6I CCWGG 3 cut(s) 63, 457, 597
PspGI CCWGG 3 cut(s) 63, 457, 597
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 1 cut(s) 359
PvuII CAGCTG 1 cut(s) 748
RsaI GTAC 3 cut(s) 281, 348, 752
RsaNI GTAC 3 cut(s) 280, 347, 751
SapI GCTCTTC 1 cut(s) 14
SaqAI TTAA 5 cut(s) 36, 117, 207, 368, 476
SatI GCNGC 1 cut(s) 469
Sau3AI GATC 1 cut(s) 359
Sau96I GGNCC 1 cut(s) 421
SchI GAGTC 1 cut(s) 755
ScrFI CCNGG 3 cut(s) 65, 459, 599
SduI GDGCHC 1 cut(s) 835
SfaNI GCATC 4 cut(s) 370, 535, 593, 693
SfcI CTRYAG 1 cut(s) 271
SmlI CTYRAG 2 cut(s) 713, 802
SmoI CTYRAG 2 cut(s) 713, 802
Sse9I AATT 9 cut(s) 76, 88, 167, 192, 339, 429, 562, 636, 871
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 1 cut(s) 732
SspMI CTAG 2 cut(s) 254, 425
StuI AGGCCT 1 cut(s) 32
StyD4I CCNGG 3 cut(s) 63, 457, 597
TaaI ACNGT 2 cut(s) 546, 674
TaqI TCGA 5 cut(s) 151, 198, 687, 859, 875
TasI AATT 9 cut(s) 76, 88, 167, 192, 339, 429, 562, 636, 871
TatI WGTACW 1 cut(s) 346
TfiI GAWTC 3 cut(s) 443, 706, 774
Tru1I TTAA 5 cut(s) 36, 117, 207, 368, 476
Tru9I TTAA 5 cut(s) 36, 117, 207, 368, 476
TscAI CASTG 2 cut(s) 902, 908
TseI GCWGC 1 cut(s) 468
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 757
TspRI CASTG 2 cut(s) 902, 908
Van91I CCANNNNNTGG 1 cut(s) 418
VneI GTGCAC 1 cut(s) 831
XapI RAATTY 2 cut(s) 192, 871
XceI RCATGY 1 cut(s) 267
XmiI GTMKAC 1 cut(s) 742
XmnI GAANNNNTTC 3 cut(s) 8, 248, 432
XspI CTAG 2 cut(s) 254, 425
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.